ViewGene
Locus Name:
GeneID:
Location:

GeneID:3604
TrivialName: PA14_14080
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14080
PAO1 Ortholog Locus:PA3859
Sequence Length:648
Protein Length:215
Start: 1207378
Stop: 1206731
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07246.1|AE004803_2 (AE004803) probable carboxylesterase [Pseudomonas aeruginosa PAO1]
Identities = 214/215 (99%), Positives = 215/215 (100%)
Sequence:
ATGAGCGAACCCCTGATCCTCGATGCCCCGAATGCCGACGCCTGCATCATCTGGCTGCACGGCCTGGGCGCC
GACCGTACCGACTTCAAGCCGGTGGCCGAAGCCCTGCAGGTGGTCCTGCCGAGCACCCGCTTCATCCTCCCC
CAGGCGCCGAGCCAGGCGGTGACGGTCAATGGCGGTTGGGTCATGCCGAGCTGGTACGACATCCTTGCCTTC
AGTCCGGCGCGGGCCATCGACGAAGACCAGTTGAACGCCTCCGCCGACCAGGTCATCGCCCTCATCGATGAA
CAGCGCGCCAAGGGCATCGCCGCCGAGCGGATCATCCTCGCCGGTTTCTCCCAGGGCGGCGCGGTGGTCCTG
CATACCGCCTTCCGCCGCTACGCCCAGCCGCTCGGCGGGGTGCTGGCGCTATCCACCTACGCGCCGACCTTC
GACGACCTGGCGCTGGACGAGCGCCACAAGCGGATTCCGGTCCTGCACCTGCATGGCAGCCAGGACGACGTG
GTCGACCCGGCGCTCGGCCGCGCGGCCCATGACGCCTTGCAGGCGCAGGGCGTGGAGGTGGGCTGGCACGAC
TACCCGATGGGCCACGAGGTGTCCCTGGAGGAAATCCACGACATCGGCGCCTGGCTGCGCAAGCGCCTCTGA
Translation:
MSEPLILDAPNADACIIWLHGLGADRTDFKPVAEALQVVLPSTRFILPQAPSQAVTVNGGWVMPSWYDILAF
SPARAIDEDQLNASADQVIALIDEQRAKGIAAERIILAGFSQGGAVVLHTAFRRYAQPLGGVLALSTYAPTF
DDLALDERHKRIPVLHLHGSQDDVVDPALGRAAHDALQAQGVEVGWHDYPMGHEVSLEEIHDIGAWLRKRL*
AnnotationID:3612GeneID:3604
AnnotatorUID: danlee
Modification Date/Time: 2006-01-27 13:01:39
Gene Name:
Confidence Code:1
GeneProduct:carboxylesterase
Cell Localization:()
Synonyms:estB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam02230,COG0400,KOG2112
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA00727.1| (D00852) esterase A [Pseudomonas fluorescens]
          Length = 218

 Score =  273 bits (699), Expect = 4e-73
 Identities = 139/217 (64%), Positives = 166/217 (76%), Gaps = 2/217 (0%)

>gb|AAO54779.1| (AE016860) carboxylesterase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 219

 Score =  271 bits (693), Expect = 2e-72
 Identities = 139/218 (63%), Positives = 166/218 (76%), Gaps = 3/218 (1%)

>gb|AAN66920.1|AE016320_8 (AE016778) carboxylesterase [Pseudomonas putida KT2440]
          Length = 218

 Score =  257 bits (657), Expect = 3e-68
 Identities = 132/217 (60%), Positives = 162/217 (74%), Gaps = 2/217 (0%)








 
Structural Features:
pfam02230, Abhydrolase_2, Phospholipase/Carboxylesterase. 
CD-Length = 216 residues, 100.0% aligned
Score =  188 bits (479), Expect = 9e-49
Identities = 87/218 (39%), Positives = 124/218 (56%), Gaps = 5/218 (2%)

COG0400, Predicted esterase 
CD-Length = 207 residues,  97.6% aligned
Score =  160 bits (406), Expect = 3e-40
Identities = 64/216 (29%), Positives = 88/216 (40%), Gaps = 15/216 (6%)

KOG2112, Lysophospholipase [Lipid transport and metabolism]
CD-Length = 206 residues,  97.1% aligned
Score =  142 bits (358), Expect = 9e-35
Identities = 75/203 (36%), Positives = 115/203 (56%), Gaps = 6/203 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7211




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24353




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31705




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3604_cdd.html


    		




 
URL:

















    ReferenceID:38273




  
Author/Investigator(s):
        Pesaresi A, Devescovi G, Lamba D, Venturi V, Degrassi G.





  
Title:
        Isolation, characterization, and heterologous expression of a carboxylesterase of Pseudomonas aeruginosa PAO1.




  
PubMed:
        15717224


MedLine:
        




    
Source:
Curr Microbiol. 2005 Feb;50(2):102-9. Epub 2005 Feb 8.






    
Reference Type: 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3604
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5774
                           PA3859_tr
                           translation of PA3859
                           215
                           99.53
                           99.99
                           0
                           1116.0
     

     
     
161189
                           gb|AAG07246.1|AE004803_2
                           (AE004803) probable carboxylesterase [Pseudomonas aeruginosa PAO1]
                           215
                           99.53
                           99.99
                           0
                           1116.0
     

     
     
161190
                           dbj|BAA00727.1|
                           (D00852) esterase A [Pseudomonas fluorescens]
                           218
                           63.76
                           76.14
                           3
                           703.0
     

     
     
161192
                           gb|AAC60403.1|
                           (S79600) esterase II [Pseudomonas fluorescens]
                           218
                           63.76
                           75.22
                           3
                           694.0
     

     
     
161191
                           gb|AAO54779.1|
                           (AE016860) carboxylesterase [Pseudomonas syringae pv. tomato str. DC3000]
                           219
                           63.47
                           75.79
                           4
                           699.0
     

     






      

          
GC ORFID: 46606How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3859Glimmer Score: 

          
Start: 3746560Stop: 3747207

          
Length: 648


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3859 translation of PA3859Homolog Bit Score 434.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4986 (ORF: BLASTX 259.0), t_PA3859 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGAACCCCTGATCCTCGATGCCCCGAATGCCGACGCCTGCATCATCTGGCTGCACGGCCTGGGCGCC 
GACCGTACCGACTTCAAGCCGGTGGCCGAAGCCCTGCAGGTGGTCCTGCCGAGCACCCGCTTCATCCTCCCC 
CAGGCGCCGAGCCAGGCGGTGACGGTCAATGGCGGTTGGGTCATGCCGAGCTGGTACGACATCCTTGCCTTC 
AGTCCGGCGCGGGCCATCGACGAAGACCAGTTGAACGCCTCCGCCGACCAGGTCATCGCCCTCATCGATGAA 
CAGCGCGCCAAGGGCATCGCCGCCGAGCGGATCATCCTCGCCGGTTTCTCCCAGGGCGGCGCGGTGGTCCTG 
CATACCGCCTTCCGCCGCTACGCCCAGCCGCTCGGCGGGGTGCTGGCGCTATCCACCTACGCGCCGACCTTC 
GACGACCTGGCGCTGGACGAGCGCCACAAGCGGATTCCGGTCCTGCACCTGCATGGCAGCCAGGACGACGTG 
GTCGACCCGGCGCTCGGCCGCGCGGCCCATGACGCCTTGCAGGCGCAGGGCGTGGAGGTGGGCTGGCACGAC 
TACCCGATGGGCCACGAGGTGTCCCTGGAGGAAATCCACGACATCGGCGCCTGGCTGCGCAAGCGCCTCTGA