ViewGene
Locus Name:
GeneID:
Location:

GeneID:3596
TrivialName: PA14_10650
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10650
PAO1 Ortholog Locus:PA4121
Sequence Length:660
Protein Length:219
Start: 918289
Stop: 917630
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07508.1|AE004828_9 (AE004828) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 216/219 (98%), Positives = 216/219 (98%)
Sequence:
ATGAGCCGCGCACTGAATGACGTCGCCGCCGGCACCCTGTTCGGCGTCGCGCTGAACTACAAGGGCCTGCTC
GACAGCCGCCTGCCGGAGTTCCAGCAGCCGCCCTACCAGAAGCCGCCGGTGAAGCCGGTGTTGTTCATCAAG
ACACCGAATACCCGCAACGAGCACGACGCCCCGGTGCTCTTCCCGCGCGGCGTCGAGCGCCTGCAACCGGGC
CCGGCGCTGGGCGTGGTGATCGCTCGCGACGCCAGCCGGGTGAAGGAGGAAGAGGCCATGGCGCATGTCGCC
GGCTACGTGATCGTCAACGAGTTCAGCCTGCCGGAAGACAGCTACTACCGCCCGGCGGTGAAAGCCAAGTGC
CGCGACGGCTTCTGCCCGTTCGGTCCGCAACTGGTGCCGACGCAGGAGATCGCCGATCCCCATGCGCTGGAG
CTGAAGCTGTACGTCAACGGCGAACTGCGCCAGCGCAACAACACCGCCAACCTGGTGCGCGACATCCCGCGG
CTGATCGCCGAGATCAGCGAGTTCATGACCCTGCACGCCGGCGACGTGCTGATCACCGGCACCCCCGAAGGG
CGCGTCGACGTGCATCCCGGCGACCGCGTCGAGGTCGAGATCGACGGCCTCGGCCGCCTCGCCAACAGCATC
GTCGCGGAATGA
Translation:
MSRALNDVAAGTLFGVALNYKGLLDSRLPEFQQPPYQKPPVKPVLFIKTPNTRNEHDAPVLFPRGVERLQPG
PALGVVIARDASRVKEEEAMAHVAGYVIVNEFSLPEDSYYRPAVKAKCRDGFCPFGPQLVPTQEIADPHALE
LKLYVNGELRQRNNTANLVRDIPRLIAEISEFMTLHAGDVLITGTPEGRVDVHPGDRVEVEIDGLGRLANSI
VAE*
AnnotationID:3604GeneID:3596
AnnotatorUID: diggins
Modification Date/Time: 2005-06-07 09:09:09
Gene Name:
Confidence Code:3
GeneProduct:putative 4-hydroxyphenylacetate degradation bifunctional isomerase
Cell Localization:()
Synonyms:hpaG1
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0179,pfam01557
Secondary Functional Category(ies):carbon compound metabolism
EC Number:
Status:ACTIVE
Pathway:
4-hydroxyphenylacetate catabolism
Homology:
>dbj|BAC14822.1| (AP004602) 4-hydroxyphenylacetate degradation bifunctional
           isomerase : decarboxylase (divided with OB2865 and
           OB2866) [Oceanobacillus iheyensis HTE831]
          Length = 263

 Score =  201 bits (512), Expect = 2e-51
 Identities = 93/209 (44%), Positives = 135/209 (64%)

>gb|AAL20033.1| (AE008747) 4-hydroxyphenylacetate catabolism protein [Salmonella
           typhimurium LT2]
          Length = 429

 Score =  185 bits (470), Expect = 1e-46
 Identities = 97/213 (45%), Positives = 134/213 (62%), Gaps = 10/213 (4%)
Structural Features:
COG0179, MhpD, 2-keto-4-pentenoate hydratase/
2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 266 residues,  80.1% aligned
Score =  218 bits (557), Expect = 4e-58
Identities = 84/219 (38%), Positives = 116/219 (52%), Gaps = 9/219 (4%)

pfam01557, FAA_hydrolase, Fumarylacetoacetate (FAA) hydrolase family.
CD-Length = 168 residues,  95.2% aligned
Score =  145 bits (367), Expect = 5e-36
Identities = 66/167 (39%), Positives = 95/167 (56%), Gaps = 6/167 (3%)
Genomic Context:






   
Comment:


















    ReferenceID:7195




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24337




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36854




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3596_cdd.html


    		




 
URL:

















    ReferenceID:36959




  
Author/Investigator(s):
        Prieto MA, Diaz E, Garcia JL.





  
Title:
        Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster.




  
PubMed:
        8550403


MedLine:
        




    
Source:
J Bacteriol. 1996 Jan;178(1):111-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8550403









           
Homologs By Global Alignment 

           

       



     
Gene ID:3596
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5765
                           PA4121_tr
                           translation of PA4121
                           219
                           98.63
                           98.63
                           0
                           1120.0
     

     
     
161048
                           gb|AAG07508.1|AE004828_9
                           (AE004828) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           219
                           98.63
                           98.63
                           0
                           1120.0
     

     
     
161061
                           emb|CAE13285.1|
                           (BX571862) hpaG1 [Photorhabdus luminescens subsp. laumondii TTO1]
                           223
                           40.80
                           54.70
                           17
                           414.0
     

     
     
161062
                           gb|AAO17177.1|
                           (AF346500) HpaG1 [Photorhabdus luminescens]
                           226
                           39.38
                           53.98
                           23
                           414.0
     

     
     
161060
                           gb|AAF12407.1|AE001863_32
                           (AE001863) 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase/5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase [Deinococcus radiodurans] [Deinococcus radiodurans R1]
                           264
                           38.25
                           51.51
                           47
                           427.0
     

     






      

          
GC ORFID: 46598How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4121Glimmer Score: 

          
Start: 4035648Stop: 4036307

          
Length: 660


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4121 translation of PA4121Homolog Bit Score 436.0

          
Other Homologs:  t_PA4121 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCGCGCACTGAATGACGTCGCCGCCGGCACCCTGTTCGGCGTCGCGCTGAACTACAAGGGCCTGCTC 
GACAGCCGCCTGCCGGAGTTCCAGCAGCCGCCCTACCAGAAGCCGCCGGTGAAGCCGGTGTTGTTCATCAAG 
ACACCGAATACCCGCAACGAGCACGACGCCCCGGTGCTCTTCCCGCGCGGCGTCGAGCGCCTGCAACCGGGC 
CCGGCGCTGGGCGTGGTGATCGCTCGCGACGCCAGCCGGGTGAAGGAGGAAGAGGCCATGGCGCATGTCGCC 
GGCTACGTGATCGTCAACGAGTTCAGCCTGCCGGAAGACAGCTACTACCGCCCGGCGGTGAAAGCCAAGTGC 
CGCGACGGCTTCTGCCCGTTCGGTCCGCAACTGGTGCCGACGCAGGAGATCGCCGATCCCCATGCGCTGGAG 
CTGAAGCTGTACGTCAACGGCGAACTGCGCCAGCGCAACAACACCGCCAACCTGGTGCGCGACATCCCGCGG 
CTGATCGCCGAGATCAGCGAGTTCATGACCCTGCACGCCGGCGACGTGCTGATCACCGGCACCCCCGAAGGG 
CGCGTCGACGTGCATCCCGGCGACCGCGTCGAGGTCGAGATCGACGGCCTCGGCCGCCTCGCCAACAGCATC 
GTCGCGGAATGA