ViewGene
Locus Name:
GeneID:
Location:

GeneID:3589
TrivialName: PA14_23760
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23760
PAO1 Ortholog Locus:PA3120
Sequence Length:639
Protein Length:212
Start: 2065008
Stop: 2065646
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06508.1|AE004736_5 (AE004736) 3-isopropylmalate dehydratase small subunit [Pseudomonas aeruginosa PAO1]
Identities = 211/212 (99%), Positives = 212/212 (100%)
Sequence:
ATGAAACCCTATACCCAGACGACCGGTCTCGTCGCTCCGCTGGATCGCGCCAACGTCGATACCGATCAGATC
ATTCCCAAGCAATTCCTCAAGTCGATCAAGCGCACCGGCTTCGGCCCCAATCTGTTCGACGAATGGCGCTAC
CTGGATGTCGGGCAGCCGGGACAGAACAACTCGAAGCGTCCGCTGAACCCGGACTTCGTCCTCAACCAGCCG
CGCTACCAGGGCGCCAGCGTACTGCTGGCGCGGGAGAACTTCGGCTGCGGCTCGTCCCGCGAGCACGCGCCC
TGGGCGCTGGACGAGTACGGTTTCCGCACGGTGATCGCACCGAGCTATGCCGACATCTTCTTCAACAACAGT
TTCAAGAACGGCTTGCTGCCGATCATCCTGCCCGAGGCCGAGGTGGACGAACTGTTCCGCCAGGTCGAGGCG
AACGAAGGCTACCAGTTGTCCATCGACCTGGCGGCGCAGACCGTTACCCGTCCTGATGGCAAGGTGCTGGGT
TTCGAGGTCGACCCGTTCCGCAAGCATTGCCTGCTCAACGGCCTCGACGACATCGGCCTGACGCTGCAGGAC
GCCGATGCGATCCGCGCCTTCGAGGACGGCTACCGCCAGCAGCAGCCCTGGCTGTTCCGCTGA
Translation:
MKPYTQTTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPGQNNSKRPLNPDFVLNQP
RYQGASVLLARENFGCGSSREHAPWALDEYGFRTVIAPSYADIFFNNSFKNGLLPIILPEAEVDELFRQVEA
NEGYQLSIDLAAQTVTRPDGKVLGFEVDPFRKHCLLNGLDDIGLTLQDADAIRAFEDGYRQQQPWLFR*
AnnotationID:3597GeneID:3589
AnnotatorUID: diggins
Modification Date/Time: 2005-04-13 07:07:26
Gene Name:leuD
Confidence Code:2
GeneProduct:3-isopropylmalate dehydratase, small subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:isopropylmalate isomerase subunit
Functional Category Confidence Code:5
COGs:COG0066
Secondary Functional Category(ies):
EC Number:4.2.1.33
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67601.1|AE016389_9 (AE016781) 3-isopropylmalate dehydratase, small subunit
           [Pseudomonas putida KT2440]
          Length = 214

 Score =  376 bits (966), Expect = e-104
 Identities = 181/212 (85%), Positives = 195/212 (91%)

>gb|AAM38300.1| (AE011992) 3-isopropylmalate dehydratase small subunit [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 215

 Score =  366 bits (939), Expect = e-101
 Identities = 173/211 (81%), Positives = 190/211 (90%)

>gb|AAC73182.1| (AE000117) isopropylmalate isomerase subunit [Escherichia coli K12]
          Length = 201

 Score =  247 bits (631), Expect = 3e-65
 Identities = 124/197 (62%), Positives = 149/197 (75%), Gaps = 8/197 (4%)






 
Structural Features:
COG0066, LeuD, 3-isopropylmalate dehydratase small subunit 
[Amino acid transport and metabolism]
CD-Length = 191 residues, 100.0% aligned
Score =  288 bits (738), Expect = 4e-79
Identities = 119/212 (56%), Positives = 144/212 (67%), Gaps = 21/212 (9%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7181




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24323




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32405




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3589_cdd.html


    		




 
URL:

















    ReferenceID:33913




  
Author/Investigator(s):
        Oultram JD, Loughlin M, Walmsley R, Gunnery SM, Minton NP.





  
Title:
        The nucleotide sequence of genes involved in the leucine biosynthetic pathway of Clostridium pasteurianum.




  
PubMed:
        8173074


MedLine:
        




    
Source:
DNA Seq. 1993;4(2):105-11.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8173074

















    ReferenceID:33914




  
Author/Investigator(s):
        Tamakoshi M, Yamagishi A, Oshima T.





  
Title:
        The organization of the leuC, leuD and leuB genes of the extreme thermophile Thermus thermophilus.




  
PubMed:
        9813279


MedLine:
        




    
Source:
Gene. 1998 Nov 5;222(1):125-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9813279









           
Homologs By Global Alignment 

           

       



     
Gene ID:3589
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5746
                           PA3120_tr
                           translation of PA3120
                           212
                           99.52
                           99.99
                           0
                           1123.0
     

     
     
160365
                           gb|AAG06508.1|AE004736_5
                           (AE004736) 3-isopropylmalate dehydratase small subunit [Pseudomonas aeruginosa PAO1]
                           212
                           99.52
                           99.99
                           0
                           1123.0
     

     
     
160366
                           emb|CAA72150.1|
                           (Y11280) LeuD protein [Azotobacter vinelandii]
                           215
                           86.04
                           91.16
                           3
                           987.0
     

     
     
160367
                           gb|AAN67601.1|AE016389_9
                           (AE016781) 3-isopropylmalate dehydratase, small subunit [Pseudomonas putida KT2440]
                           214
                           84.57
                           91.12
                           2
                           966.0
     

     
     
160368
                           gb|AAO55691.1|
                           (AE016863) 3-isopropylmalate dehydratase, small subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           213
                           83.56
                           89.20
                           1
                           942.0
     

     






      

          
GC ORFID: 46591How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3120Glimmer Score: 

          
Start: 2888923Stop: 2888285

          
Length: 639


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3120 translation of PA3120Homolog Bit Score 437.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene969 (ORF: BLASTX 372.0), t_PA3120 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACCCTATACCCAGACGACCGGTCTCGTCGCTCCGCTGGATCGCGCCAACGTCGATACCGATCAGATC 
ATTCCCAAGCAATTCCTCAAGTCGATCAAGCGCACCGGCTTCGGCCCCAATCTGTTCGACGAATGGCGCTAC 
CTGGATGTCGGGCAGCCGGGACAGAACAACTCGAAGCGTCCGCTGAACCCGGACTTCGTCCTCAACCAGCCG 
CGCTACCAGGGCGCCAGCGTACTGCTGGCGCGGGAGAACTTCGGCTGCGGCTCGTCCCGCGAGCACGCGCCC 
TGGGCGCTGGACGAGTACGGTTTCCGCACGGTGATCGCACCGAGCTATGCCGACATCTTCTTCAACAACAGT 
TTCAAGAACGGCTTGCTGCCGATCATCCTGCCCGAGGCCGAGGTGGACGAACTGTTCCGCCAGGTCGAGGCG 
AACGAAGGCTACCAGTTGTCCATCGACCTGGCGGCGCAGACCGTTACCCGTCCTGATGGCAAGGTGCTGGGT 
TTCGAGGTCGACCCGTTCCGCAAGCATTGCCTGCTCAACGGCCTCGACGACATCGGCCTGACGCTGCAGGAC 
GCCGATGCGATCCGCGCCTTCGAGGACGGCTACCGCCAGCAGCAGCCCTGGCTGTTCCGCTGA