ViewGene
Locus Name:
GeneID:
Location:

GeneID:358
TrivialName: PA14_66620
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66620
PAO1 Ortholog Locus:PA5040
Sequence Length:2133
Protein Length:710
Start: 5949248
Stop: 5947116
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08425.1|AE004917_4 (AE004917) Type 4 fimbrial biogenesis outer membrane protein PilQ precursor [Pseudomonas aeruginosa PAO1]
Identities = 677/714 (94%), Positives = 693/714 (97%), Gaps = 4/714 (0%)
Sequence:
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCG
GACCTGGAGAAACTCGACGTGGCGGCACTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCC
GTCGCCGCGCCGCGTGGCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAAC
AAACTCGGCACCAAGAATCGCGAGCTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGAT
CGCACACGGTTGATCATCAACCTGACCACGCTGTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTC
GTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTTCCGCCGCTCCGGTCAAGGCAAGCCCGGCTCCG
GCTAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGTCGTGCCATCCGCAACATCGATTTC
CAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTTGAGCCCGGATATTCAA
GAACAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCGGATGCGCTGCGGGTTCGCCTGGAC
GTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAACCGAGCGGGCTCTACGACTATCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATG
ACCACGGAAGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGTGAGAAACTGTCGCTGAACTTC
CAGGACATCGACGTGCGTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGAT
ACCGTGCAGGGCAATATCACCCTGCGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAG
ACCAAGGGCCTGGACAAGCGCAAGCTCGGCAACGTGCTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGT
GAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGTTGGCGCCACTGCGTCGGGAACTGATCCAGGTG
AACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTTACTAGTAGTGGCGAAGAGAAAGAAAAT
TCTCGTGGTTCGATTACCGTAGATGACCGTACCAACAGCATCATTGCCTATCAGCCGCAGGAGCGGCTGGAC
GAGCTACGCCGAATCGTTTCCCAGTTGGACATCCCGGTTCGTCAGGTGATGATCGAGGCCCGTATCGTCGAG
GCCAACGTGGGGTACGACAAGAGTCTTGGTGTGCGTTGGGGTGGTGCCTACCACAATGGCAACTGGAACAGC
TACGGCAAGAACGGCAACATGGGCATCAAGGATAAGGAAGGTTACAACTGCGGGCCGTTCCAGGGGCAGTGT
ACGTTCCCGACCAGAGATAATTCTCCGACCCCATTTGTGGATATGGGGGCCAAGGATGCTAGTTCCGGTATC
GGTATTGGCTTTATCACCGATAACATCATTCTTGACTTGCAATTATCGGCTATGGAGAAAACCGGGAACGGC
GAAGTGGTTTCGCAACCCAAGGTCGTAACTTCTGACAAGGAAACCGCGAAGATCCTAAAGGGATCGGAGATC
CCATATCAGGAGGCCAGTTCCAGTGGTGCGACCAGCACCTCGTTCAAGGAGGCCGCTCTTTCTCTGGAGGTG
ACGCCGCAGATCACTCCAGACAATCGGATCATCGTTGAGGTGAAGGTGACCAAGGATGCTCCCGACTTCCAG
AATGCTTTGAATGGAGTTCCTCCGATCAACAAGAACGAGGTTAACGCCAAGATTCTGGTCAACGATGGTGAA
ACCATCGTTATCGGCGGTGTATTCAGCAACACCCAGACAAAGGCTGTCGACAAGGTACCCTTCCTCGGTGAC
CTGCCTTACTTGGGCCGTTTGTTCCGCCGCGATACGGTCTCCGATACGAAAAACGAACTTCTGGTTTTCCTG
ACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA
Translation:
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQN
KLGTKNRELSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASAAPVKASPAP
ASYAQPIKPKPYVPAGRAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLD
VKDFATPVQFVNASAQSDRTSITIEPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNF
QDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAAR
ERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVTSSGEEKENSRGSITVDDRTNSIIAYQPQERLD
ELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHNGNWNSYGKNGNMGIKDKEGYNCGPFQGQC
TFPTRDNSPTPFVDMGAKDASSGIGIGFITDNIILDLQLSAMEKTGNGEVVSQPKVVTSDKETAKILKGSEI
PYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFQNALNGVPPINKNEVNAKILVNDGE
TIVIGGVFSNTQTKAVDKVPFLGDLPYLGRLFRRDTVSDTKNELLVFLTPRIMNNQAIAIGR*
AnnotationID:367GeneID:358
AnnotatorUID: diggins
Modification Date/Time: 2005-03-14 17:05:58
Gene Name:pilQ
Confidence Code:1
GeneProduct:type 4 fimbrial biogenesis outer membrane protein PilQ precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:fimbrial assembly protein
Functional Category Confidence Code:5
COGs:COG4796,pfam00263
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58555.1| (AE016874) type IV pilus biogenesis protein PilQ [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 718

 Score =  998 bits (2581), Expect = 0.0
 Identities = 523/715 (73%), Positives = 603/715 (84%), Gaps = 27/715 (3%)

>gb|AAN70645.1|AE016708_6 (AE016793) type IV pili biogenesis protein PilQ [Pseudomonas putida
           KT2440]
          Length = 420

 Score =  493 bits (1269), Expect = e-138
 Identities = 256/433 (59%), Positives = 329/433 (75%), Gaps = 36/433 (8%)








 
Structural Features:
COG4796, HofQ, Type II secretory pathway, component HofQ 
[Intracellular trafficking and secretion]
CD-Length = 709 residues, 100.0% aligned
Score =  538 bits (1387), Expect = 9e-154
Identities = 248/719 (34%), Positives = 345/719 (47%), Gaps = 26/719 (3%)

pfam00263, Secretin_C, Bacterial type II and III secretion system protein.
CD-Length = 232 residues, 100.0% aligned
Score =  203 bits (517), Expect = 6e-53
Identities = 86/260 (33%), Positives = 130/260 (50%), Gaps = 34/260 (13%)






   
Genomic Context:






   
Comment:


















    ReferenceID:719




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17861




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35014




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:358_cdd.html


    		




 
URL:

















    ReferenceID:35844




  
Author/Investigator(s):
        Martin PR, Hobbs M, Free PD, Jeske Y, Mattick JS.





  
Title:
        Characterization of pilQ, a new gene required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa.




  
PubMed:
        7901733


MedLine:
        




    
Source:
Mol Microbiol. 1993 Aug;9(4):857-68.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7901733









           
Homologs By Global Alignment 

           

       



     
Gene ID:358
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
602
                           PA5040_tr
                           translation of PA5040
                           714
                           94.81
                           97.05
                           4
                           3399.0
     

     
     
28251
                           gb|AAG08425.1|AE004917_4
                           (AE004917) Type 4 fimbrial biogenesis outer membrane protein PilQ precursor [Pseudomonas aeruginosa PAO1]
                           714
                           94.81
                           97.05
                           4
                           3399.0
     

     
     
28252
                           gb|AAA16704.1|
                           (L13865) fimbrial assembly protein [Pseudomonas aeruginosa]
                           727
                           91.74
                           94.08
                           17
                           3332.0
     

     
     
28253
                           gb|AAO58555.1|
                           (AE016874) type IV pilus biogenesis protein PilQ [Pseudomonas syringae pv. tomato str. DC3000]
                           738
                           71.27
                           82.11
                           48
                           2607.0
     

     
     
28254
                           gb|AAK00351.1|AF329876_7
                           (AF329876) putative outer membrane protein ComQ [Acinetobacter sp. BD413]
                           765
                           39.47
                           56.60
                           97
                           1378.0
     

     






      

          
GC ORFID: 43360How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5040Glimmer Score: 

          
Start: 5212931Stop: 5210799

          
Length: 2133


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5040 translation of PA5040Homolog Bit Score 1311.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3294 (ORF: BLASTX 1011.0), t_PA5040 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCG 
GACCTGGAGAAACTCGACGTGGCGGCACTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCC 
GTCGCCGCGCCGCGTGGCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAAC 
AAACTCGGCACCAAGAATCGCGAGCTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGAT 
CGCACACGGTTGATCATCAACCTGACCACGCTGTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTC 
GTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTTCCGCCGCTCCGGTCAAGGCAAGCCCGGCTCCG 
GCTAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGTCGTGCCATCCGCAACATCGATTTC 
CAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTTGAGCCCGGATATTCAA 
GAACAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCGGATGCGCTGCGGGTTCGCCTGGAC 
GTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC 
GAACCGAGCGGGCTCTACGACTATCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATG 
ACCACGGAAGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGTGAGAAACTGTCGCTGAACTTC 
CAGGACATCGACGTGCGTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGAT 
ACCGTGCAGGGCAATATCACCCTGCGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAG 
ACCAAGGGCCTGGACAAGCGCAAGCTCGGCAACGTGCTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGT 
GAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGTTGGCGCCACTGCGTCGGGAACTGATCCAGGTG 
AACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTTACTAGTAGTGGCGAAGAGAAAGAAAAT 
TCTCGTGGTTCGATTACCGTAGATGACCGTACCAACAGCATCATTGCCTATCAGCCGCAGGAGCGGCTGGAC 
GAGCTACGCCGAATCGTTTCCCAGTTGGACATCCCGGTTCGTCAGGTGATGATCGAGGCCCGTATCGTCGAG 
GCCAACGTGGGGTACGACAAGAGTCTTGGTGTGCGTTGGGGTGGTGCCTACCACAATGGCAACTGGAACAGC 
TACGGCAAGAACGGCAACATGGGCATCAAGGATAAGGAAGGTTACAACTGCGGGCCGTTCCAGGGGCAGTGT 
ACGTTCCCGACCAGAGATAATTCTCCGACCCCATTTGTGGATATGGGGGCCAAGGATGCTAGTTCCGGTATC 
GGTATTGGCTTTATCACCGATAACATCATTCTTGACTTGCAATTATCGGCTATGGAGAAAACCGGGAACGGC 
GAAGTGGTTTCGCAACCCAAGGTCGTAACTTCTGACAAGGAAACCGCGAAGATCCTAAAGGGATCGGAGATC 
CCATATCAGGAGGCCAGTTCCAGTGGTGCGACCAGCACCTCGTTCAAGGAGGCCGCTCTTTCTCTGGAGGTG 
ACGCCGCAGATCACTCCAGACAATCGGATCATCGTTGAGGTGAAGGTGACCAAGGATGCTCCCGACTTCCAG 
AATGCTTTGAATGGAGTTCCTCCGATCAACAAGAACGAGGTTAACGCCAAGATTCTGGTCAACGATGGTGAA 
ACCATCGTTATCGGCGGTGTATTCAGCAACACCCAGACAAAGGCTGTCGACAAGGTACCCTTCCTCGGTGAC 
CTGCCTTACTTGGGCCGTTTGTTCCGCCGCGATACGGTCTCCGATACGAAAAACGAACTTCTGGTTTTCCTG 
ACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA