ViewGene
Locus Name:
GeneID:
Location:

GeneID:3579
TrivialName: PA14_22000
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_22000
PAO1 Ortholog Locus:PA3246
Sequence Length:636
Protein Length:211
Start: 1914441
Stop: 1913806
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06634.1|AE004747_7 (AE004747) pseudouridine synthase RluA [Pseudomonas aeruginosa PAO1]
Identities = 210/211 (99%), Positives = 211/211 (100%)
Sequence:
ATGCCGCTGTCCCAGATTCGAATCGTTCACCAGGATGACGCCCTGCTGGTGGTCGACAAGCCCACCCTGCTG
CTGTCGGTGCCCGGCCGCGCCGATGACAACAAGGACTGCCTGGTCACCCGCCTGCAGGAAAACGGCTATCCC
GAGGCCCGCATCGTCCACCGCCTGGACTGGGAAACCTCCGGCCTGATGGTGCTCGCCCGCGACGCCGACAGC
CACCGCGAGCTGTCCCGCCAGTTCCACGACCGCGAGACCGAGAAAGCCTATACCGCCCTTTGCTGGGGCCTG
CCGGAACTGGACAGCGGGCGCATCGAACTGCCGCTGCGCTACGACCCGCCGACCAAGCCGCGTCACGTGGTG
GACTTCGAGCACGGCCGCCACGCCCTGACCTTCTGGCGTGTGGTCGAGCGTCATGAGCGCTGGAGCAGGGTC
GAGCTGACGCCGATCACCGGCCGTTCGCACCAGTTGCGCGTGCACATGCTCTCCATCGGCCATCCGCTGCTC
GGCGACCGCCTCTACGCCAACCCCGAAGCCCTCGCCGCCCACGAACGCCTGTGCCTGCACGCCAGCCTGCTG
TGCCTGACCCATCCGCAGAGCGGCGAACGCCTGCGCTTCGAGTCGCCGGCGCCGTTCTGA
Translation:
MPLSQIRIVHQDDALLVVDKPTLLLSVPGRADDNKDCLVTRLQENGYPEARIVHRLDWETSGLMVLARDADS
HRELSRQFHDRETEKAYTALCWGLPELDSGRIELPLRYDPPTKPRHVVDFEHGRHALTFWRVVERHERWSRV
ELTPITGRSHQLRVHMLSIGHPLLGDRLYANPEALAAHERLCLHASLLCLTHPQSGERLRFESPAPF*
AnnotationID:3587GeneID:3579
AnnotatorUID: diggins
Modification Date/Time: 2005-04-14 14:02:58
Gene Name:rluA
Confidence Code:2
GeneProduct:ribosomal large subunit pseudouridine synthase A
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(24) Transcription, RNA processing and degradation
Alternate Gene Product Name:pseudouridylate synthase
Functional Category Confidence Code:5
COGs:pfam00849
Secondary Functional Category(ies):translation, ribosomal structure and biogenesis
EC Number:4.2.1.70
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN78565.1|AE016755_65 (AE016755) Ribosomal large subunit pseudouridine synthase A
           [Escherichia coli CFT073]
          Length = 219

 Score =  206 bits (523), Expect = 1e-52
 Identities = 105/205 (51%), Positives = 144/205 (70%), Gaps = 2/205 (0%)

>emb|CAC89354.1| (AJ414143) ribosomal large subunit pseudouridine synthase A
           [Yersinia pestis CO92]
          Length = 206

 Score =  203 bits (516), Expect = 6e-52
 Identities = 106/205 (51%), Positives = 143/205 (69%), Gaps = 2/205 (0%)






 
Structural Features:
pfam00849, PseudoU_synth_2, RNA pseudouridylate synthase. 
CD-Length = 151 residues,  98.0% aligned
Score =  128 bits (322), Expect = 7e-31
Identities = 64/148 (43%), Positives = 91/148 (61%), Gaps = 2/148 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7161




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24303




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32296




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3579_cdd.html


    		




 
URL:

















    ReferenceID:33987




  
Author/Investigator(s):
        del Campo M, Ofengand J, Malhotra A.





  
Title:
        Crystal structure of the catalytic domain of RluD, the only rRNA pseudouridine synthase required for normal growth of Escherichia coli.




  
PubMed:
        14730022


MedLine:
        




    
Source:
RNA. 2004 Feb;10(2):231-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14730022









           
Homologs By Global Alignment 

           

       



     
Gene ID:3579
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5726
                           PA3246_tr
                           translation of PA3246
                           211
                           99.52
                           99.99
                           0
                           1130.0
     

     
     
159739
                           gb|AAG06634.1|AE004747_7
                           (AE004747) pseudouridine synthase RluA [Pseudomonas aeruginosa PAO1]
                           211
                           99.52
                           99.99
                           0
                           1130.0
     

     
     
159745
                           emb|CAC89354.1|
                           (AJ414143) ribosomal large subunit pseudouridine synthase A [Yersinia pestis CO92]
                           212
                           50.00
                           67.92
                           7
                           518.0
     

     
     
159744
                           gb|AAM87226.1|AE013971_2
                           (AE013971) hypothetical protein [Yersinia pestis KIM]
                           212
                           50.00
                           67.92
                           7
                           518.0
     

     
     
159740
                           gb|AAQ61413.1|
                           (AE016923) pseudouridine synthase A [Chromobacterium violaceum ATCC 12472]
                           226
                           49.55
                           66.37
                           16
                           544.0
     

     






      

          
GC ORFID: 46581How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3246Glimmer Score: 

          
Start: 3039490Stop: 3040125

          
Length: 636


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3246 translation of PA3246Homolog Bit Score 439.0

          
Other Homologs:  psyr_15may02_Scaffold16_revised_gene2672 (ORF: BLASTX 375.0), t_PA3246 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCGCTGTCCCAGATTCGAATCGTTCACCAGGATGACGCCCTGCTGGTGGTCGACAAGCCCACCCTGCTG 
CTGTCGGTGCCCGGCCGCGCCGATGACAACAAGGACTGCCTGGTCACCCGCCTGCAGGAAAACGGCTATCCC 
GAGGCCCGCATCGTCCACCGCCTGGACTGGGAAACCTCCGGCCTGATGGTGCTCGCCCGCGACGCCGACAGC 
CACCGCGAGCTGTCCCGCCAGTTCCACGACCGCGAGACCGAGAAAGCCTATACCGCCCTTTGCTGGGGCCTG 
CCGGAACTGGACAGCGGGCGCATCGAACTGCCGCTGCGCTACGACCCGCCGACCAAGCCGCGTCACGTGGTG 
GACTTCGAGCACGGCCGCCACGCCCTGACCTTCTGGCGTGTGGTCGAGCGTCATGAGCGCTGGAGCAGGGTC 
GAGCTGACGCCGATCACCGGCCGTTCGCACCAGTTGCGCGTGCACATGCTCTCCATCGGCCATCCGCTGCTC 
GGCGACCGCCTCTACGCCAACCCCGAAGCCCTCGCCGCCCACGAACGCCTGTGCCTGCACGCCAGCCTGCTG 
TGCCTGACCCATCCGCAGAGCGGCGAACGCCTGCGCTTCGAGTCGCCGGCGCCGTTCTGA