ViewGene
Locus Name:
GeneID:
Location:

GeneID:3578
TrivialName: PA14_23090
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23090
PAO1 Ortholog Locus:PA3181
Sequence Length:663
Protein Length:220
Start: 2002349
Stop: 2003011
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06569.1|AE004742_5 (AE004742) 2-keto-3-deoxy-6-phosphogluconate aldolase [Pseudomonas aeruginosa PAO1]
Identities = 219/220 (99%), Positives = 220/220 (100%)
Sequence:
ATGCACAACCTTGAACAGAAGACCGCCCGCATCGACACGCTGTGCCGGGAGGCGCGCATCCTCCCGGTGATC
ACCATCGACCGCGAGGCGGACATCCTGCCGATGGCCGATGCCCTCGCCGCCGGCGGCCTGACCGCCCTGGAG
ATCACCCTGCGCACGGCACACGGGCTGACCGCCATCCGTCGCCTCAGCGAGGAGCGCCCGCACCTGCGCATC
GGCGCCGGCACCGTGCTCGACCCGCAGACCTTCGCCGCCGCGGAAAAGGCCGGGGCGAGCTTCGTGGTCACC
CCGGGATGCACTGACGAGTTGCTGCGCTTCGCCCTGGACAGCGAAGTCCCGCTGTTGCCCGGCGTGGCCAGC
GCTTCCGAGATCATGCTCGCCTACCGCCATGGCTACCGCCGCTTCAAGCTGTTTCCCGCCGAGGTCAGCGGC
GGCCCGGCGGCGCTGAAGGCGTTCTCGGGACCATTCCCCGATATCCGCTTCTGCCCCACCGGAGGCGTCAGC
CTGAACAATCTCGCCGACTACCTGGCGGTACCCAACGTGATGTGCGTCGGCGGCACCTGGATGCTGCCCAAG
GCCGTGGTCGACCGCGGCGACTGGGCCCAGGTCGAGCGCCTCAGCCGCGAAGCCCTGGAGCGCTTCGCCGAG
CACCGCAGACACTGA
Translation:
MHNLEQKTARIDTLCREARILPVITIDREADILPMADALAAGGLTALEITLRTAHGLTAIRRLSEERPHLRI
GAGTVLDPQTFAAAEKAGASFVVTPGCTDELLRFALDSEVPLLPGVASASEIMLAYRHGYRRFKLFPAEVSG
GPAALKAFSGPFPDIRFCPTGGVSLNNLADYLAVPNVMCVGGTWMLPKAVVDRGDWAQVERLSREALERFAE
HRRH*
AnnotationID:3586GeneID:3578
AnnotatorUID: danlee
Modification Date/Time: 2006-01-31 16:04:25
Gene Name:
Confidence Code:2
GeneProduct:2-keto-3-deoxy-6-phosphogluconate aldolase
Cell Localization:()
Synonyms:PA3181, edaA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:2-dehydro-3-deoxyphosphogluconate
Functional Category Confidence Code:5
COGs:pfam01081,COG0800
Secondary Functional Category(ies):central intermediary metabolism
EC Number:4.1.2.14
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66649.1|AE016292_9 (AE016777) 2-dehydro-3-deoxyphosphogluconate
           aldolase/4-hydroxy-2-oxoglutarate aldolase [Pseudomonas
           putida KT2440]
          Length = 235

 Score =  281 bits (718), Expect = 3e-75
 Identities = 134/209 (64%), Positives = 166/209 (79%)

>gb|AAO54826.1| (AE016860) 4-hydroxy-2-oxoglutarate
           aldolase/2-dehydro-3-deoxyphosphogluconate [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 224

 Score =  277 bits (708), Expect = 4e-74
 Identities = 135/212 (63%), Positives = 168/212 (79%)

>emb|CAC90879.1| (AJ414151) KHG/KDPG aldolase [Yersinia pestis CO92]
          Length = 213

 Score =  207 bits (527), Expect = 4e-53
 Identities = 93/193 (48%), Positives = 139/193 (72%)








 
Structural Features:
pfam01081, Aldolase, KDPG and KHG aldolase. 
CD-Length = 196 residues, 100.0% aligned
Score =  255 bits (652), Expect = 4e-69
Identities = 106/196 (54%), Positives = 146/196 (74%)

COG0800, Eda, 2-keto-3-deoxy-6-phosphogluconate aldolase 
[Carbohydrate transport and metabolism]
CD-Length = 211 residues, 100.0% aligned
Score =  246 bits (630), Expect = 1e-66
Identities = 101/212 (47%), Positives = 136/212 (64%), Gaps = 2/212 (0%)







   
Genomic Context:






   
Comment:


















    ReferenceID:7159




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24301




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32364




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3578_cdd.html


    		




 
URL:

















    ReferenceID:33960




  
Author/Investigator(s):
        Petruschka L, Adolf K, Burchhardt G, Dernedde J, Jurgensen J, Herrmann H.





  
Title:
        Analysis of the zwf-pgl-eda-operon in Pseudomonas putida strains H and KT2440.




  
PubMed:
        12393206


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2002 Sep 24;215(1):89-95.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12393206

















    ReferenceID:33961




  
Author/Investigator(s):
        Temple L, Cuskey SM, Perkins RE, Bass RC, Morales NM, Christie GE, Olsen RH, Phibbs PV Jr.





  
Title:
        Analysis of cloned structural and regulatory genes for carbohydrate utilization in Pseudomonas aeruginosa PAO.




  
PubMed:
        2121713


MedLine:
        




    
Source:
J Bacteriol. 1990 Nov;172(11):6396-402.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2121713

















    ReferenceID:33962




  
Author/Investigator(s):
        Cuskey SM, Wolff JA, Phibbs PV Jr, Olsen RH.





  
Title:
        Cloning of genes specifying carbohydrate catabolism in Pseudomonas aeruginosa and Pseudomonas putida.




  
PubMed:
        3922954


MedLine:
        




    
Source:
J Bacteriol. 1985 Jun;162(3):865-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3922954









           
Homologs By Global Alignment 

           

       



     
Gene ID:3578
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5724
                           PA3181_tr
                           translation of PA3181
                           220
                           99.54
                           99.99
                           0
                           1128.0
     

     
     
159682
                           gb|AAG06569.1|AE004742_5
                           (AE004742) 2-keto-3-deoxy-6-phosphogluconate aldolase [Pseudomonas aeruginosa PAO1]
                           220
                           99.54
                           99.99
                           0
                           1128.0
     

     
     
159683
                           gb|AAC38312.2|
                           (AF029673) 2-keto-3-deoxy-6-phosphogluconate aldolase [Pseudomonas aeruginosa]
                           220
                           99.54
                           99.99
                           0
                           1128.0
     

     
     
159685
                           emb|CAC14910.1|
                           (AJ279003) 2-keto-3-deoxy-6-phosphogluconate aldolase [Pseudomonas putida]
                           229
                           59.82
                           73.36
                           12
                           708.0
     

     
     
159686
                           gb|AAO54826.1|
                           (AE016860) 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxyphosphogluconate [Pseudomonas syringae pv. tomato str. DC3000]
                           229
                           58.95
                           73.79
                           14
                           706.0
     

     






      

          
GC ORFID: 46580How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3181Glimmer Score: 

          
Start: 2951582Stop: 2950920

          
Length: 663


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3181 translation of PA3181Homolog Bit Score 439.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4426 (ORF: BLASTX 275.0), t_PA3131 (ORF: BLASTX 190.0), t_PA3181 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCACAACCTTGAACAGAAGACCGCCCGCATCGACACGCTGTGCCGGGAGGCGCGCATCCTCCCGGTGATC 
ACCATCGACCGCGAGGCGGACATCCTGCCGATGGCCGATGCCCTCGCCGCCGGCGGCCTGACCGCCCTGGAG 
ATCACCCTGCGCACGGCACACGGGCTGACCGCCATCCGTCGCCTCAGCGAGGAGCGCCCGCACCTGCGCATC 
GGCGCCGGCACCGTGCTCGACCCGCAGACCTTCGCCGCCGCGGAAAAGGCCGGGGCGAGCTTCGTGGTCACC 
CCGGGATGCACTGACGAGTTGCTGCGCTTCGCCCTGGACAGCGAAGTCCCGCTGTTGCCCGGCGTGGCCAGC 
GCTTCCGAGATCATGCTCGCCTACCGCCATGGCTACCGCCGCTTCAAGCTGTTTCCCGCCGAGGTCAGCGGC 
GGCCCGGCGGCGCTGAAGGCGTTCTCGGGACCATTCCCCGATATCCGCTTCTGCCCCACCGGAGGCGTCAGC 
CTGAACAATCTCGCCGACTACCTGGCGGTACCCAACGTGATGTGCGTCGGCGGCACCTGGATGCTGCCCAAG 
GCCGTGGTCGACCGCGGCGACTGGGCCCAGGTCGAGCGCCTCAGCCGCGAAGCCCTGGAGCGCTTCGCCGAG 
CACCGCAGACACTGA