ViewGene

Locus Name:
GeneID:
Location:

GeneID: 3575

TrivialName: PA14_35860

AnnotatorUID: ericdeziel

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_35860

PAO1 Ortholog Locus:

Sequence Length: 1305

Protein Length: 434

Start: 3190988

Stop: 3192292

Strand: +

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAK01507.1| (AF241171) putative amino acid/amine transport protein [Pseudomonas aeruginosa] Identities = 429/434 (98%), Positives = 430/434 (99%) to gene #17 of PAGI-1 island.

Sequence:

TTGAAGCGCACGCTGGGTCCGTTTTCCGTTCTGCTGTTCGGTCTGGCGTTCCTCGCTCCGTTGATCGTGTTC
GGCACCTATGGCGTGATCACGCAGGCCAGCGGCAACACCACGGCGATGGCCTACCTGATCGCGGCAATCGGC
GTGGTGTTCACGGCCTTGAGCTATGGCCGGCTGGTCAGGGTGTTCCCGGTCGCCGGTTCGGCCTACACCTAT
ACCCGCAAGATGCTGAGCGCGAACCTCGGCTTCATGGTGGGCTGGGCCGCCCTGCTCGATTACTTCTTCATC
CCGATGCTGATCTGGCTGCTCGGCGCCAGCTACCTCAACATGGCCTTTCCAGAGGTGCCGCAATGGGTGTGG
ATCATCGGCTTCATCGTCTCCACCTCGCTGCTTAATATCCTGGGCATCCAGGTCGCCAACCGCTTCAACGTG
CTGTTGATGGTCGCGCAACTGGCCATCATCGTCGTGTTCATCAGCCTGTGCGTGCACTACATCGTCGCGGCC
AACGGCCCTGGCGGGCTGCTGTCGGCCAAGCCGTTCTTCAACCCGGACGTGTCCTTCGCCACCAGCATGGCC
GGCGCCGCCATTGCCGCGTACTCGTTCCTGGGCTTCGACGCGCTGTCCACGCTCAGCGAGGAGACCCGCGAC
CCGGGCCGGACACTGCCGCGCGCGATCCTGCTGGTCGCATTGATCGGCGGCTCGGTCTACGTCGGCTCGTCG
TACTTCATGTACCTGGCGCATCCCTCCCCCGCCTTCGAGCATGTGGACGGCGCCGCCTTCGAGATCGCCCGC
ATGATCGGCGGCGACCTGTTCTTCGCCGTGGTCCTGGCGGGGATCATCATCGCCCACTTCGCCGCAGGCATG
TCGTTCCAGGCCAGCGTGGGCCGGCTGCTGTACGCCCTGGGGCGGGACAACCAACTGCCGCGCAAGCTGTTC
GGTGCCCTGCATCCTCGCTACAAGACCCCGGCCTTCAACATTCTGCTCTGCGGCATTTTCGGCTGCGCGGGT
TTTGCCCTGACGATCGCCACCGCGACGTCCCTGGTCAATTTCGGCGCGTTTCTCGCCTTCAGCGCAGTCAAC
CTTTGCGCGTTGCGGCTCTCCTTCGATACCCGCCTCGGAGACGGGGCGGGCTTGCTGCACGGGGTGCTCTGC
CCGCTCGTTGGCCTGGTCACGGCCGGGTGGATGCTGGTCAGCCTGGATAACGACGCGCTGATCATGGGCTGT
AGCTGGCTGGGCCTGGGGGCGGTCTATCTCGCTTGCCGGACCAGCTTCTTCCGCAACCCGGTGCCCGACGCG
CTGGCGTAG

Translation:

MKRTLGPFSVLLFGLAFLAPLIVFGTYGVITQASGNTTAMAYLIAAIGVVFTALSYGRLVRVFPVAGSAYTY
TRKMLSANLGFMVGWAALLDYFFIPMLIWLLGASYLNMAFPEVPQWVWIIGFIVSTSLLNILGIQVANRFNV
LLMVAQLAIIVVFISLCVHYIVAANGPGGLLSAKPFFNPDVSFATSMAGAAIAAYSFLGFDALSTLSEETRD
PGRTLPRAILLVALIGGSVYVGSSYFMYLAHPSPAFEHVDGAAFEIARMIGGDLFFAVVLAGIIIAHFAAGM
SFQASVGRLLYALGRDNQLPRKLFGALHPRYKTPAFNILLCGIFGCAGFALTIATATSLVNFGAFLAFSAVN
LCALRLSFDTRLGDGAGLLHGVLCPLVGLVTAGWMLVSLDNDALIMGCSWLGLGAVYLACRTSFFRNPVPDA
LA*

AnnotationID:3583 GeneID:3575

AnnotatorUID: danlee

Modification Date/Time: 2005-10-11 17:05:00

Gene Name: PAGI-1(17)

Confidence Code: 3

GeneProduct: Probable amino acid permease

Cell Localization: ()

Synonyms:

Cell Localization Confidence Code: 5

MolecularFunction:

Functional Category: (27) Transport of small molecules

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs:

Secondary Functional Category(ies):

EC Number:

Status: ACTIVE

Pathway:

Homology:

gi|12698393|gb|AAK01507.1|  putative amino acid/amine transport protein [Pseudomonas aeruginosa]
     Length=443
     Score =  847 bits (2188),  Expect = 0.0
     Identities = 429/434 (98%), Positives = 430/434 (99%), Gaps = 0/434 (0%)

gi|24984394|gb|AAN68410.1|  amino acid permease family protein [Pseudomonas putida KT2440]
     Length=443
     Score =  790 bits (2041),  Expect = 0.0
     Identities = 398/433 (91%), Positives = 411/433 (94%), Gaps = 0/433 (0%)

Structural Features:

pfam00324, AA_permease, Amino acid permease.
             CD-Length = 473 residues,  81.0% aligned
             Score =  108 bits (271), Expect = 1e-24

COG0531, PotE, Amino acid transporters [Amino acid transport and metabolism].
             CD-Length = 466 residues,  80.9% aligned
             Score =  103 bits (257), Expect = 5e-23

Genomic Context:

Predicted gene #16 of the PAGI-1 island absent in PAO1 but present in a majority of pathogenic P. aeruginosa isolates.   
The PA14 island at this locus, from PA14_35860 to PA14_36030, corresponds to PAGI-1 genes 17 through 2, respectively.

Comment:

The first half of the PA14 island (from PA14_35700 through PA14_35850) diverges from PAGI-1 such that 
genes 18 through 51 are absent.  PA14 has 31 ORFs in 35368 bp (from PA14_35700 to PA14_36030), 
whereas PAGI-1 contains 51 ORFs in 51300 bp.

ReferenceID:14885

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:24295

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:38207

Author/Investigator(s): Liang X, Pham XQ, Olson MV, Lory S.

Title: Identification of a genomic island present in the majority of pathogenic isolates of Pseudomonas aeruginosa.

PubMed: 11208781

MedLine:

Source:

J Bacteriol. 2001 Feb;183(3):843-53.

Reference Type: Journal

Data:

URL:

Homologs By Global Alignment

Gene ID:3575

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

159229 gb|AAK01507.1| (AF241171) putative amino acid/amine transport protein [Pseudomonas aeruginosa] 443 97.06 97.06 9 2190.0

159230 gb|AAN68410.1|AE016473_8 (AE016784) amino acid permease family protein [Pseudomonas putida KT2440] 443 90.06 92.77 9 2043.0

5715 PA3597_tr translation of PA3597 444 50.00 67.79 14 1129.0

159232 gb|AAG06985.1|AE004780_4 (AE004780) probable amino acid permease [Pseudomonas aeruginosa PAO1] 444 50.00 67.79 14 1129.0

159231 dbj|BAC69064.1| (AP005026) putative amino acid permease [Streptomyces avermitilis MA-4680] 463 49.24 63.93 29 1154.0

GC ORFID: `46577`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA3597`	Glimmer Score:
Start: `1762928`	Stop: `1761624`
Length: `1305`
Start Codon: `TTG`	Truncated Start:
Stop Codon: `TAG`	Truncated Stop:
Homolog: `t_PA3597 translation of PA3597`	Homolog Bit Score `439.0`
Other Homologs: `t_PA2041 (ORF: BLASTX 375.0), t_PA3597 (ORF: GLIMMER)`
GC ORF Sequence
TTGAAGCGCACGCTGGGTCCGTTTTCCGTTCTGCTGTTCGGTCTGGCGTTCCTCGCTCCGTTGATCGTGTTC GGCACCTATGGCGTGATCACGCAGGCCAGCGGCAACACCACGGCGATGGCCTACCTGATCGCGGCAATCGGC GTGGTGTTCACGGCCTTGAGCTATGGCCGGCTGGTCAGGGTGTTCCCGGTCGCCGGTTCGGCCTACACCTAT ACCCGCAAGATGCTGAGCGCGAACCTCGGCTTCATGGTGGGCTGGGCCGCCCTGCTCGATTACTTCTTCATC CCGATGCTGATCTGGCTGCTCGGCGCCAGCTACCTCAACATGGCCTTTCCAGAGGTGCCGCAATGGGTGTGG ATCATCGGCTTCATCGTCTCCACCTCGCTGCTTAATATCCTGGGCATCCAGGTCGCCAACCGCTTCAACGTG CTGTTGATGGTCGCGCAACTGGCCATCATCGTCGTGTTCATCAGCCTGTGCGTGCACTACATCGTCGCGGCC AACGGCCCTGGCGGGCTGCTGTCGGCCAAGCCGTTCTTCAACCCGGACGTGTCCTTCGCCACCAGCATGGCC GGCGCCGCCATTGCCGCGTACTCGTTCCTGGGCTTCGACGCGCTGTCCACGCTCAGCGAGGAGACCCGCGAC CCGGGCCGGACACTGCCGCGCGCGATCCTGCTGGTCGCATTGATCGGCGGCTCGGTCTACGTCGGCTCGTCG TACTTCATGTACCTGGCGCATCCCTCCCCCGCCTTCGAGCATGTGGACGGCGCCGCCTTCGAGATCGCCCGC ATGATCGGCGGCGACCTGTTCTTCGCCGTGGTCCTGGCGGGGATCATCATCGCCCACTTCGCCGCAGGCATG TCGTTCCAGGCCAGCGTGGGCCGGCTGCTGTACGCCCTGGGGCGGGACAACCAACTGCCGCGCAAGCTGTTC GGTGCCCTGCATCCTCGCTACAAGACCCCGGCCTTCAACATTCTGCTCTGCGGCATTTTCGGCTGCGCGGGT TTTGCCCTGACGATCGCCACCGCGACGTCCCTGGTCAATTTCGGCGCGTTTCTCGCCTTCAGCGCAGTCAAC CTTTGCGCGTTGCGGCTCTCCTTCGATACCCGCCTCGGAGACGGGGCGGGCTTGCTGCACGGGGTGCTCTGC CCGCTCGTTGGCCTGGTCACGGCCGGGTGGATGCTGGTCAGCCTGGATAACGACGCGCTGATCATGGGCTGT AGCTGGCTGGGCCTGGGGGCGGTCTATCTCGCTTGCCGGACCAGCTTCTTCCGCAACCCGGTGCCCGACGCG CTGGCGTAG