ViewGene
Locus Name:
GeneID:
Location:

GeneID:3571
TrivialName: PA14_27460
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_27460
PAO1 Ortholog Locus:PA2832
Sequence Length:657
Protein Length:218
Start: 2383567
Stop: 2384223
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06220.1|AE004710_2 (AE004710) thiopurine methyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 214/218 (98%), Positives = 216/218 (99%)
Sequence:
ATGCAGGCGGATTTTTGGCACGCCCGCTGGGCGAACAACCAGATCGGCTTCCACCTGGACGAGATCAATCCC
TACCTGATGCGCCACCTGTCGCGGCTGCGATTGCAAGCGGGCGAACGGATCCTGGTGCCGTTGTGCGGCAAG
ACCCTGGACCTGGCCTGGCTGGCCGCCCAGGGGCTGGAGGTGCTGGGGGTGGAGCTTTCGGAAAAGGCCGTG
AGCGACTTCTTCGAGGAGCACGACCTGCGCCCCGAGATCGATCAACTGGGTGGTTTCCGCCGCTACCGGGTC
GCCGGCATCACCCTGCTGCAGGGTGATTTCTTCGCCTTGCAGGCAGAGCACCTGGCGCAGTGCAGGGCGTTC
TACGACCGCGCCGCGCTGATCGCCCTGCCGCCGGAGATGCGCGAGCGCTATGCCGGGCATCTCCAGGCGGTC
CTGCCGACGCGCAGCCTCGGCTTGCTGGTCACCATCGACTACCCGCAGGCGGAGATGGCCGGTCCGCCGTTC
GCCGTGCCCGACGAGGAGGTGCGTGGCTACTACGCTGGCGGTTGGCGGATCGAGGAACTGGAGCGCGGCGAC
GTGCTCGGCGTCAACTGGAAATTCCTCGAGCGCGGGGTGTCCTGGCTGAACGAGGCCGTCTACCTGCTGGAG
AGAGGCTGA
Translation:
MQADFWHARWANNQIGFHLDEINPYLMRHLSRLRLQAGERILVPLCGKTLDLAWLAAQGLEVLGVELSEKAV
SDFFEEHDLRPEIDQLGGFRRYRVAGITLLQGDFFALQAEHLAQCRAFYDRAALIALPPEMRERYAGHLQAV
LPTRSLGLLVTIDYPQAEMAGPPFAVPDEEVRGYYAGGWRIEELERGDVLGVNWKFLERGVSWLNEAVYLLE
RG*
AnnotationID:3579GeneID:3571
AnnotatorUID: diggins
Modification Date/Time: 2005-04-06 15:03:31
Gene Name:tpm
Confidence Code:2
GeneProduct:thiopurine methyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam05724
Secondary Functional Category(ies):
EC Number:2.1.1.67
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAP12368.1| (AY259120) thiopurine methyltransferase [Pseudomonas sp. Hsa.28]
          Length = 218

 Score =  273 bits (697), Expect = 7e-73
 Identities = 130/217 (59%), Positives = 158/217 (72%)

>gb|AAN67489.1|AE016375_7 (AE016780) thiopurine s-methyltransferase [Pseudomonas putida
           KT2440]
          Length = 216

 Score =  230 bits (586), Expect = 5e-60
 Identities = 116/217 (53%), Positives = 150/217 (69%), Gaps = 1/217 (0%)








 
Structural Features:
pfam05724, TPMT, Thiopurine S-methyltransferase (TPMT). 
CD-Length = 218 residues, 100.0% aligned
Score =  284 bits (729), Expect = 5e-78
Identities = 113/218 (51%), Positives = 138/218 (63%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7145




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24287




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36246




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3571_cdd.html


    		




 
URL:

















    ReferenceID:36400




  
Author/Investigator(s):
        Ranjard L, Prigent-Combaret C, Nazaret S, Cournoyer B.





  
Title:
        Methylation of inorganic and organic selenium by the bacterial thiopurine methyltransferase.




  
PubMed:
        12003960


MedLine:
        




    
Source:
J Bacteriol. 2002 Jun;184(11):3146-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12003960

















    ReferenceID:36401




  
Author/Investigator(s):
        Cournoyer B, Watanabe S, Vivian A.





  
Title:
        A tellurite-resistance genetic determinant from phytopathogenic pseudomonads encodes a thiopurine methyltransferase: evidence of a widely-conserved family of methyltransferases.




  
PubMed:
        9565678


MedLine:
        




    
Source:
Biochim Biophys Acta. 1998 Apr 29;1397(2):161-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=98234304&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:3571
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5711
                           PA2832_tr
                           translation of PA2832
                           218
                           98.16
                           99.08
                           0
                           1131.0
     

     
     
159210
                           gb|AAG06220.1|AE004710_2
                           (AE004710) thiopurine methyltransferase [Pseudomonas aeruginosa PAO1]
                           218
                           98.16
                           99.08
                           0
                           1131.0
     

     
     
159211
                           gb|AAP12368.1|
                           (AY259120) thiopurine methyltransferase [Pseudomonas sp. Hsa.28]
                           218
                           59.63
                           72.47
                           0
                           696.0
     

     
     
159212
                           gb|AAN67489.1|AE016375_7
                           (AE016780) thiopurine s-methyltransferase [Pseudomonas putida KT2440]
                           218
                           53.21
                           68.80
                           2
                           588.0
     

     
     
159215
                           emb|CAE41176.1|
                           (BX640413) thiopurine S-methyltransferase [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           219
                           48.85
                           62.55
                           1
                           517.0
     

     






      

          
GC ORFID: 46573How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2832Glimmer Score: 

          
Start: 2570360Stop: 2569704

          
Length: 657


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2832 translation of PA2832Homolog Bit Score 440.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7758 (ORF: BLASTX 202.0), t_PA2832 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAGGCGGATTTTTGGCACGCCCGCTGGGCGAACAACCAGATCGGCTTCCACCTGGACGAGATCAATCCC 
TACCTGATGCGCCACCTGTCGCGGCTGCGATTGCAAGCGGGCGAACGGATCCTGGTGCCGTTGTGCGGCAAG 
ACCCTGGACCTGGCCTGGCTGGCCGCCCAGGGGCTGGAGGTGCTGGGGGTGGAGCTTTCGGAAAAGGCCGTG 
AGCGACTTCTTCGAGGAGCACGACCTGCGCCCCGAGATCGATCAACTGGGTGGTTTCCGCCGCTACCGGGTC 
GCCGGCATCACCCTGCTGCAGGGTGATTTCTTCGCCTTGCAGGCAGAGCACCTGGCGCAGTGCAGGGCGTTC 
TACGACCGCGCCGCGCTGATCGCCCTGCCGCCGGAGATGCGCGAGCGCTATGCCGGGCATCTCCAGGCGGTC 
CTGCCGACGCGCAGCCTCGGCTTGCTGGTCACCATCGACTACCCGCAGGCGGAGATGGCCGGTCCGCCGTTC 
GCCGTGCCCGACGAGGAGGTGCGTGGCTACTACGCTGGCGGTTGGCGGATCGAGGAACTGGAGCGCGGCGAC 
GTGCTCGGCGTCAACTGGAAATTCCTCGAGCGCGGGGTGTCCTGGCTGAACGAGGCCGTCTACCTGCTGGAG 
AGAGGCTGA