ViewGene
Locus Name:
GeneID:
Location:

GeneID:3565
TrivialName: PA14_37950
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_37950
PAO1 Ortholog Locus:PA2053
Sequence Length:663
Protein Length:220
Start: 3387041
Stop: 3387703
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05441.1|AE004631_10 (AE004631) carbonate dehydratase [Pseudomonas aeruginosa PAO1]
Identities = 219/220 (99%), Positives = 220/220 (100%)
Sequence:
ATGCGTGACATCATCGACGGCTTTCTCCGCTTCCAGCGCGACGCCTACCCGGCGCGCTCGCAACTGTTCAAG
TCCCTGGCCACCCGGCAAGCCCCCAAGGCGCTGTTCATCGCCTGCTCGGACAGCCGGGTGGTCCCCGAGCTG
CTGACCCAGCGCGAGCCCGGCGAACTCTTCGTGATCCGTAATGCCGGCAACATCGTCCCCGGCTACGGGCCG
CAGCCCGGCGGGGTCAGCGCCTCGGTGGAATACGCGGTGGCGGTGCTCGGGGTCGGCGACATCGTGGTCTGC
GGCCACTCGGACTGCGGCGCGATGGGCGCCATCGCGTCCTGCGCCTGTCTCGACCAGTTGCCGGCGGTGGCC
GGCTGGTTGCACCACGCCGAGGCCGCACGGGCGATGAACAGCGCCCACGAACACGCCTCCGAGGCGGCGCGG
CTGGACGCCCTGGTGCGGCACAACGTGATCGCCCAACTGGCCAACCTGCGCACCCACCCCTGCGTCGCCCGC
GCCCTGGAACAGGGTCGGCTGAACCTGCACGGCTGGGTCTACGACATCGAAAGCGGCCGCATCGACGCCCTC
GACGGCGCCAGCCGGCGCTTCGTCTCCCTCGCCGAGCATCCCGAAGTGCGCGCGGTAGGCGGCGACCCCGGG
CAGGCGGTCGCCTGA
Translation:
MRDIIDGFLRFQRDAYPARSQLFKSLATRQAPKALFIACSDSRVVPELLTQREPGELFVIRNAGNIVPGYGP
QPGGVSASVEYAVAVLGVGDIVVCGHSDCGAMGAIASCACLDQLPAVAGWLHHAEAARAMNSAHEHASEAAR
LDALVRHNVIAQLANLRTHPCVARALEQGRLNLHGWVYDIESGRIDALDGASRRFVSLAEHPEVRAVGGDPG
QAVA*
AnnotationID:3573GeneID:3565
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 17:05:00
Gene Name:cynT
Confidence Code:2
GeneProduct:carbonate dehydratase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:carbonic anhydrase
Functional Category Confidence Code:5
COGs:COG0288,pfam00484
Secondary Functional Category(ies):transport of small molecules
EC Number:4.2.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAG54688.1|AE005213_4 (AE005213) carbonic anhydrase [Escherichia coli O157:H7 EDL933]
          Length = 219

 Score =  301 bits (771), Expect = 2e-81
 Identities = 143/211 (67%), Positives = 176/211 (83%)

>gb|AAN65734.1|AE016199_6 (AE016774) carbonic anhydrase [Pseudomonas putida KT2440]
          Length = 239

 Score =  226 bits (575), Expect = 1e-58
 Identities = 109/204 (53%), Positives = 141/204 (69%), Gaps = 1/204 (0%)

>gb|AAM40825.1| (AE012254) carbonic anhydrase [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 226

 Score =  199 bits (506), Expect = 1e-50
 Identities = 99/201 (49%), Positives = 129/201 (64%), Gaps = 3/201 (1%)






 
Structural Features:
COG0288, CynT, Carbonic anhydrase [Inorganic ion transport and metabolism]
CD-Length = 207 residues,  98.6% aligned
Score =  202 bits (515), Expect = 3e-53
Identities = 101/208 (48%), Positives = 128/208 (61%), Gaps = 8/208 (3%)

pfam00484, Pro_CA, Carbonic anhydrase
CD-Length = 164 residues, 100.0% aligned
Score =  157 bits (398), Expect = 1e-39
Identities = 66/172 (38%), Positives = 90/172 (52%), Gaps = 13/172 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7133




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24275




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32651




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3565_cdd.html


    		




 
URL:

















    ReferenceID:34402




  
Author/Investigator(s):
        Guilloton MB, Korte JJ, Lamblin AF, Fuchs JA, Anderson PM.





  
Title:
        Carbonic anhydrase in Escherichia coli. A product of the cyn operon.




  
PubMed:
        1740425


MedLine:
        




    
Source:
J Biol Chem. 1992 Feb 25;267(6):3731-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1740425

















    ReferenceID:34403




  
Author/Investigator(s):
        Anderson PM, Sung YC, Fuchs JA.





  
Title:
        The cyanase operon and cyanate metabolism.




  
PubMed:
        2094285


MedLine:
        




    
Source:
FEMS Microbiol Rev. 1990 Dec;7(3-4):247-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2094285

















    ReferenceID:34404




  
Author/Investigator(s):
        Sung YC, Fuchs JA.





  
Title:
        Characterization of the cyn operon in Escherichia coli K12.




  
PubMed:
        3049588


MedLine:
        




    
Source:
J Biol Chem. 1988 Oct 15;263(29):14769-75.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3049588









           
Homologs By Global Alignment 

           

       



     
Gene ID:3565
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5685
                           PA2053_tr
                           translation of PA2053
                           220
                           99.54
                           99.99
                           0
                           1135.0
     

     
     
158325
                           gb|AAG05441.1|AE004631_10
                           (AE004631) carbonate dehydratase [Pseudomonas aeruginosa PAO1]
                           220
                           99.54
                           99.99
                           0
                           1135.0
     

     
     
158332
                           gb|AAQ59555.1|
                           (AE016916) carbonate dehydratase [Chromobacterium violaceum ATCC 12472]
                           221
                           66.51
                           78.28
                           3
                           747.0
     

     
     
158326
                           gb|AAG54688.1|AE005213_4
                           (AE005213) carbonic anhydrase [Escherichia coli O157:H7 EDL933]
                           221
                           65.61
                           80.54
                           3
                           765.0
     

     
     
158327
                           gb|AAC73442.1|
                           (AE000141) carbonic anhydrase [Escherichia coli K12]
                           221
                           65.61
                           80.54
                           3
                           765.0
     

     






      

          
GC ORFID: 46567How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2053Glimmer Score: 

          
Start: 1566870Stop: 1566208

          
Length: 663


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2053 translation of PA2053Homolog Bit Score 441.0

          
Other Homologs:  t_PA0102 (ORF: BLASTX 221.0), psyr_15may02_Scaffold2_revised_gene3880 (ORF: BLASTX 220.0), t_PA2053 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGTGACATCATCGACGGCTTTCTCCGCTTCCAGCGCGACGCCTACCCGGCGCGCTCGCAACTGTTCAAG 
TCCCTGGCCACCCGGCAAGCCCCCAAGGCGCTGTTCATCGCCTGCTCGGACAGCCGGGTGGTCCCCGAGCTG 
CTGACCCAGCGCGAGCCCGGCGAACTCTTCGTGATCCGTAATGCCGGCAACATCGTCCCCGGCTACGGGCCG 
CAGCCCGGCGGGGTCAGCGCCTCGGTGGAATACGCGGTGGCGGTGCTCGGGGTCGGCGACATCGTGGTCTGC 
GGCCACTCGGACTGCGGCGCGATGGGCGCCATCGCGTCCTGCGCCTGTCTCGACCAGTTGCCGGCGGTGGCC 
GGCTGGTTGCACCACGCCGAGGCCGCACGGGCGATGAACAGCGCCCACGAACACGCCTCCGAGGCGGCGCGG 
CTGGACGCCCTGGTGCGGCACAACGTGATCGCCCAACTGGCCAACCTGCGCACCCACCCCTGCGTCGCCCGC 
GCCCTGGAACAGGGTCGGCTGAACCTGCACGGCTGGGTCTACGACATCGAAAGCGGCCGCATCGACGCCCTC 
GACGGCGCCAGCCGGCGCTTCGTCTCCCTCGCCGAGCATCCCGAAGTGCGCGCGGTAGGCGGCGACCCCGGG 
CAGGCGGTCGCCTGA