ViewGene
Locus Name:
GeneID:
Location:

GeneID:3564
TrivialName: PA14_41950
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41950
PAO1 Ortholog Locus:PA1748
Sequence Length:690
Protein Length:229
Start: 3744661
Stop: 3745350
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05137.1|AE004601_2 (AE004601) probable enoyl-CoA hydratase/isomerase [Pseudomonas aeruginosa PAO1]
Identities = 229/229 (100%), Positives = 229/229 (100%)
Sequence:
ATGAGCGAGTTGATCAGCTACCAGTTCGAAGACGGCATCGCCACCCTGACCCTCAACAACGGCAAGGTGAAC
GCCATCTCCCCGGCGGTGATCGACGCCTTCAACCAGGCTCTCGACCAGGCCTTGCAGGACAAGGCCGTGGTC
ATCGTCACCGGCCAGCCGGGCATCCTCTCCGGTGGCTACGACCTGAAGGTCATGACCTCCGGCCCGGAGAAT
GCCGTCAACCTGGTCGCTGCCGGCTCGACCCTGGCTCGCCGCCTGCTCTCCCATCCCTACCCGGTGATCGTC
GCCTGCCCCGGGCACGCGGTAGCCAAGGGCGCCTTCCTGCTGCTCTCGGCCGACTACCGGATCGGCGTCGAC
GGGCCGTTCCAGATCGGCCTGAACGAAGTCGCCATCGGCATGACCATGCACCATGTCGGCATCGAACTGGCC
CGCGATCGCCTGCGCAAGTCGGCGTTCACCCGCTCGGTCATCAATGCCGAGATGTTCAGTCCGGCCGCTGCG
GTGGATGCCGGCTTCCTCGACACCCTGGTCAGCGCCGACCAGTTGCAGGAAAGCGCGCGTGCAGCGGCCCAG
CAGTTGAAGAAACTCAACATGACCGCGCACCGCAATACCAAGCTCAAGGTGCGCAAGGCACTGCTGGAAACC
CTCGACCAGGCGATCGAGCTGGACAAGAAGCACCTGGGCTAA
Translation:
MSELISYQFEDGIATLTLNNGKVNAISPAVIDAFNQALDQALQDKAVVIVTGQPGILSGGYDLKVMTSGPEN
AVNLVAAGSTLARRLLSHPYPVIVACPGHAVAKGAFLLLSADYRIGVDGPFQIGLNEVAIGMTMHHVGIELA
RDRLRKSAFTRSVINAEMFSPAAAVDAGFLDTLVSADQLQESARAAAQQLKKLNMTAHRNTKLKVRKALLET
LDQAIELDKKHLG*
AnnotationID:3572GeneID:3564
AnnotatorUID: diggins
Modification Date/Time: 2005-06-02 07:07:37
Gene Name:
Confidence Code:3
GeneProduct:putative enoyl-CoA hydratase/isomerase
Cell Localization:()
Synonyms:PA1748
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1024,pfam00378
Secondary Functional Category(ies):central intermediary metabolism
EC Number:4.2.1.17
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67464.1|AE016373_6 (AE016780) enoyl-CoA hydratase/isomerase family protein
           [Pseudomonas putida KT2440]
          Length = 229

 Score =  357 bits (916), Expect = 3e-98
 Identities = 178/229 (77%), Positives = 206/229 (89%)

>gb|AAO55583.1| (AE016863) enoyl-CoA hydratase/isomerase family protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 229

 Score =  349 bits (896), Expect = 6e-96
 Identities = 176/228 (77%), Positives = 198/228 (86%)








 
Structural Features:
COG1024, CaiD, Enoyl-CoA hydratase/carnithine racemase [Lipid metabolism]
CD-Length = 257 residues,  90.7% aligned
Score =  117 bits (294), Expect = 1e-27
Identities = 79/235 (33%), Positives = 114/235 (48%), Gaps = 8/235 (3%)

pfam00378, ECH, Enoyl-CoA hydratase/isomerase family.
CD-Length = 168 residues, 100.0% aligned
Score = 82.2 bits (203), Expect = 5e-17
Identities = 51/169 (30%), Positives = 77/169 (45%), Gaps = 4/169 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:7131




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24273




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37140




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3564_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3564
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5684
                           PA1748_tr
                           translation of PA1748
                           229
                           99.99
                           99.99
                           0
                           1134.0
     

     
     
158322
                           gb|AAG05137.1|AE004601_2
                           (AE004601) probable enoyl-CoA hydratase/isomerase [Pseudomonas aeruginosa PAO1]
                           229
                           99.99
                           99.99
                           0
                           1134.0
     

     
     
158323
                           gb|AAN67464.1|AE016373_6
                           (AE016780) enoyl-CoA hydratase/isomerase family protein [Pseudomonas putida KT2440]
                           229
                           77.72
                           89.95
                           0
                           916.0
     

     
     
158324
                           gb|AAO55583.1|
                           (AE016863) enoyl-CoA hydratase/isomerase family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           229
                           76.85
                           86.46
                           0
                           892.0
     

     






      

          
GC ORFID: 46566How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1748Glimmer Score: 

          
Start: 1209248Stop: 1208559

          
Length: 690


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA1748 translation of PA1748Homolog Bit Score 441.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene540 (ORF: BLASTX 346.0), t_PA1748 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGAGTTGATCAGCTACCAGTTCGAAGACGGCATCGCCACCCTGACCCTCAACAACGGCAAGGTGAAC 
GCCATCTCCCCGGCGGTGATCGACGCCTTCAACCAGGCTCTCGACCAGGCCTTGCAGGACAAGGCCGTGGTC 
ATCGTCACCGGCCAGCCGGGCATCCTCTCCGGTGGCTACGACCTGAAGGTCATGACCTCCGGCCCGGAGAAT 
GCCGTCAACCTGGTCGCTGCCGGCTCGACCCTGGCTCGCCGCCTGCTCTCCCATCCCTACCCGGTGATCGTC 
GCCTGCCCCGGGCACGCGGTAGCCAAGGGCGCCTTCCTGCTGCTCTCGGCCGACTACCGGATCGGCGTCGAC 
GGGCCGTTCCAGATCGGCCTGAACGAAGTCGCCATCGGCATGACCATGCACCATGTCGGCATCGAACTGGCC 
CGCGATCGCCTGCGCAAGTCGGCGTTCACCCGCTCGGTCATCAATGCCGAGATGTTCAGTCCGGCCGCTGCG 
GTGGATGCCGGCTTCCTCGACACCCTGGTCAGCGCCGACCAGTTGCAGGAAAGCGCGCGTGCAGCGGCCCAG 
CAGTTGAAGAAACTCAACATGACCGCGCACCGCAATACCAAGCTCAAGGTGCGCAAGGCACTGCTGGAAACC 
CTCGACCAGGCGATCGAGCTGGACAAGAAGCACCTGGGCTAA