ViewGene
Locus Name:
GeneID:
Location:

GeneID:3560
TrivialName: PA14_67920
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67920
PAO1 Ortholog Locus:PA5142
Sequence Length:642
Protein Length:213
Start: 6063253
Stop: 6062612
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08527.1|AE004927_5 (AE004927) glutamine amidotransferase [Pseudomonas aeruginosa PAO1]
Identities = 213/213 (100%), Positives = 213/213 (100%)
Sequence:
GTGATGCAGACGGTAGCCGTGATCGACTACGGCATGGGCAACCTGCACTCGGTGGCCAAGGCCCTCGAGCAC
GTCGGTGCCGGCCGGGTGCTGGTGAGCAGCGACGCGGCAGTGATCCGCGAGGCCGACCGGGTGGTGTTCCCC
GGGGTCGGCGCGATCCGCGACTGCATGGCCGAGATCCGCCGCCTGGGCTTCGACGCCCTGGTCCGCGAGGTC
AGCCAGGACCGCCCGTTCCTCGGCATCTGCGTCGGCATGCAGGCATTGCTGGAGCGCAGCGAGGAGAACGAC
GGGGTCGACTGCATCGGTCTGTTCCCCGGCCAGGTGCGCTTCTTCGGCAAGGACCTGCACGAAGCCGGCGAG
CACCTGAAGGTGCCGCACATGGGCTGGAACCAGGTGAGCCAGGCGGTGGAGCACCCGCTGTGGCACGAGATT
CCCGACCAGGCGCGCTTCTACTTCGTGCACAGCTACTACATCGAGGCCGGCAATCCGCGCCAGGTGGTGGGG
CACGGGCACTACGGCGTCGATTTCGCCGCCGCGCTGGCCGAAGGCTCGCGTTTCGCCGTGCAGTTCCACCCG
GAAAAGAGCCATACCCACGGCCTGCAACTGCTGCAGAACTTCGTGGCCTGGGACGGGCGCTGGTAG
Translation:
MMQTVAVIDYGMGNLHSVAKALEHVGAGRVLVSSDAAVIREADRVVFPGVGAIRDCMAEIRRLGFDALVREV
SQDRPFLGICVGMQALLERSEENDGVDCIGLFPGQVRFFGKDLHEAGEHLKVPHMGWNQVSQAVEHPLWHEI
PDQARFYFVHSYYIEAGNPRQVVGHGHYGVDFAAALAEGSRFAVQFHPEKSHTHGLQLLQNFVAWDGRW*
AnnotationID:3568GeneID:3560
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 13:01:31
Gene Name:hisH1
Confidence Code:2
GeneProduct:lutamine amidotransferase class-I
Cell Localization:()
Synonyms:hisH
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:glutamine amidotransferase
Functional Category Confidence Code:5
COGs:COG0118,pfam00117
Secondary Functional Category(ies):amino acid transport and metabolism
EC Number:2.4.2.-
Status:ACTIVE
Pathway:






 
Homology:
COG0118, HisH, Glutamine amidotransferase 
[Amino acid transport and metabolism]
CD-Length = 204 residues, 100.0% aligned
Score =  282 bits (722), Expect = 3e-77
Identities = 110/211 (52%), Positives = 134/211 (63%), Gaps = 8/211 (3%)

pfam00117, GATase, Glutamine amidotransferase class-I.
CD-Length = 188 residues,  99.5% aligned
Score =  101 bits (252), Expect = 8e-23
Identities = 53/214 (24%), Positives = 86/214 (40%), Gaps = 37/214 (17%)






 
Structural Features:






   
Genomic Context:






   
Comment:


















    ReferenceID:14879




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24265




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34946




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3560_cdd.html


    		




 
URL:

















    ReferenceID:35784




  
Author/Investigator(s):
        Burrows LL, Charter DF, Lam JS.





  
Title:
        Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster.




  
PubMed:
        8939432


MedLine:
        




    
Source:
Mol Microbiol. 1996 Nov;22(3):481-95.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8939432

















    ReferenceID:35785




  
Author/Investigator(s):
        Erbeznik M, Strobel HJ, Dawson KA.





  
Title:
        Organization and sequence of histidine biosynthesis genes hisH, -A, -F, and -IE in Thermoanaerobacter ethanolicus.




  
PubMed:
        10594232


MedLine:
        




    
Source:
Curr Microbiol. 2000 Feb;40(2):140-2.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10594232









           
Homologs By Global Alignment 

           

       



     
Gene ID:3560
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5679
                           PA5142_tr
                           translation of PA5142
                           213
                           99.99
                           99.99
                           0
                           1138.0
     

     
     
158147
                           gb|AAG08527.1|AE004927_5
                           (AE004927) glutamine amidotransferase [Pseudomonas aeruginosa PAO1]
                           213
                           99.53
                           99.99
                           0
                           1135.0
     

     
     
158148
                           gb|AAN65921.1|AE016220_3
                           (AE016775) glutamine amidotransferase HisH [Pseudomonas putida KT2440]
                           213
                           90.14
                           95.30
                           1
                           1048.0
     

     
     
158149
                           gb|AAO58763.1|
                           (AE016875) amidotransferase HisH [Pseudomonas syringae pv. tomato str. DC3000]
                           213
                           89.20
                           95.77
                           1
                           1039.0
     

     
     
158150
                           gb|AAQ58292.1|
                           (AE016912) amidophosphoribosyltransferase [Chromobacterium violaceum ATCC 12472]
                           216
                           51.85
                           61.57
                           7
                           537.0
     

     






      

          
GC ORFID: 46562How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5142Glimmer Score: 

          
Start: 5326938Stop: 5326297

          
Length: 642


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA5142 translation of PA5142Homolog Bit Score 442.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6484 (ORF: BLASTX 406.0), t_PA3152 (ORF: BLASTX 154.0), t_PA5142 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGATGCAGACGGTAGCCGTGATCGACTACGGCATGGGCAACCTGCACTCGGTGGCCAAGGCCCTCGAGCAC 
GTCGGTGCCGGCCGGGTGCTGGTGAGCAGCGACGCGGCAGTGATCCGCGAGGCCGACCGGGTGGTGTTCCCC 
GGGGTCGGCGCGATCCGCGACTGCATGGCCGAGATCCGCCGCCTGGGCTTCGACGCCCTGGTCCGCGAGGTC 
AGCCAGGACCGCCCGTTCCTCGGCATCTGCGTCGGCATGCAGGCATTGCTGGAGCGCAGCGAGGAGAACGAC 
GGGGTCGACTGCATCGGTCTGTTCCCCGGCCAGGTGCGCTTCTTCGGCAAGGACCTGCACGAAGCCGGCGAG 
CACCTGAAGGTGCCGCACATGGGCTGGAACCAGGTGAGCCAGGCGGTGGAGCACCCGCTGTGGCACGAGATT 
CCCGACCAGGCGCGCTTCTACTTCGTGCACAGCTACTACATCGAGGCCGGCAATCCGCGCCAGGTGGTGGGG 
CACGGGCACTACGGCGTCGATTTCGCCGCCGCGCTGGCCGAAGGCTCGCGTTTCGCCGTGCAGTTCCACCCG 
GAAAAGAGCCATACCCACGGCCTGCAACTGCTGCAGAACTTCGTGGCCTGGGACGGGCGCTGGTAG