ViewGene
Locus Name:
GeneID:
Location:

GeneID:356
TrivialName: PA14_00090
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_00090
PAO1 Ortholog Locus:PA0008
Sequence Length:2055
Protein Length:684
Start: 12488
Stop: 10434
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03398.1|AE004440_8 (AE004440) glycyl-tRNA synthetase beta chain [Pseudomonas aeruginosa PAO1]
Identities = 682/684 (99%), Positives = 683/684 (99%)
Sequence:
ATGAGCGCCAAGGATTTCCTGGTCGAACTGGGCACCGAAGAGCTGCCGCCAAAAGCCCTCAACAGCCTCGGC
GAAGCCTTCCTCAGCGGTATCGAGAAGGGCCTCAAGGCTGCCGGTCTGAGTTACGCCGCTGCGCGTTTCTAT
GCGGCGCCGCGCCGTCTCGCCGTGCTGGTCGAGCAACTCGCCGTGCAGCAGCCCGACCGTACCGTCAACCTA
GACGGTCCGCCGCTACAGGCCGCCTTCGACGCCAGCGGTAACCCGACCCAGGCCGCCCTGGGCTTCGCCAAG
AAGTGCGGCGTGGATCTACAGCAGATCGACAAGAGCGGTCCGAAGCTGCGCTTCAGCCAGACCATCGCCGGC
CAACCGGCCGCCGGCCTGCTGCCGGGCATCGTCGAGGCCTCGTTGAACGAACTGCCGATTCCCAAGCGGATG
CGCTGGGCAGCTCGCCGCGAGGAGTTCGTGCGTCCGACCCAATGGCTGGTGATGCTGTTCGGCGACGACGTG
GTCGAGTGCGAGATCCTCGCCCAGAAAGCCGGGCGCGAATCCCGTGGCCACCGCTTCCACAATCCGGACAAT
GTGCGTATTTCCAGCCCGGCAGCGTACCTGGAAGACCTGCGCGGCGCTCATGTGCTGGCCGATTTCGCCGAG
CGCCGCGAGCTGATCGCCAAGCGCGTGGCAGAACTGGCCGCCGAGCAACAGGGCAGCGCCATCGTGCCGCCG
AGCCTGCTCGACGAAGTGACTGCACTGGTCGAGTGGCCGGTGCCGCTGGTCTGCTCCTTCGAGGAGCGCTTC
CTCGAGGTCCCGCAGGAAGCCCTGATCACCACCATGCAGGACAACCAGAAATACTTCTGCCTGCTGGACGCC
AACGGCAAGCTGCTGCCGCGCTTCATCACCGTCGCCAACGTCGAGAGCAAGGCGCCGGAGAACATCGTCAGC
GGTAACGAGAAGGTGGTTCGTCCGCGCCTCACCGACGCCGAGTTCTTCTTCAAGCAGGACAAGAAGCAGCCG
CTCGAAAGCTTCAACGAGCGCCTGCGCAACGTAGTGTTCCAGGCCCAGCTCGGCACCGTGTTCGAGAAGGCC
CAGCGCGTCTCCGGCCTGGCCGCCTACATCGCCGAACGTATAGGCGGCAATGCGCAGAACGCCGCGCGCGCC
GGCATCCTGTCCAAGTGCGACCTGGCGACCGAGATGGTCGGCGAGTTCCCCGAGATGCAGGGCATCGCCGGC
TACTACTACGCCACCCACGGCGGCGAAGCGGAAGACGTCGCCCTGGCCCTCAACGAGCAGTACATGCCGCGC
GGCGCCGGCGCCGAGCTACCCTCGACCCTGACCGGCGCCGCCGTGGCGGTGGCCGACAAGCTCGATACCCTG
GTCGGTATCTTCGGCATCGGCATGCTTCCCACCGGCAGCAAGGACCCCTACGCGCTGCGCCGCGCTGCGCTG
GGCGTGCTGCGCATCCTCATCGAGAAGCAGCTCGACCTGGACCTGGTAGCCGCGGTCAACGCCGCCGTCGAG
CAATATGGCGACAAGGTCAAGGCCGCCGGCCTGGCCGAGCAGGTGCTGGACTTCGTGTTCGACCGCCTGCGC
GCGCGCTACGAGGACGAAGGCGTGGACGTGGCCGTGTACCAGTCGGTGCGCGCGCTCAAGCCAAGCTCGCCG
CTGGACTTCGACCAGCGCGTACAGGCCGTCCAGGCCTTCCGCCAGTTGCCTGAAGCCGAGGCCCTGGCCGCG
GCGAACAAACGGGTGTCGAATATTCTCGCCAAGTCCGAGGACGAGGTTCCGCCGAACGTGGATGCCAGCCTG
CTGGTGGAAGCTGCCGAGAAGGCCCTGGGCAGCGCCGTGGCGAACGCCGAGAGCGAAGTCGCGCCGCTGGCA
GCGGCACGCGACTATCGCGCCGCGCTGGCCCGCCTGGCGGCCTTGCGCGAGCCGGTGGATACGTTCTTCGCC
GATGTGATGGTCAATGTCGACGACGCGGCGGTGCGCGCCAACCGCTATGCGCTGCTGGCCAAGCTGCGCGGG
TCGTTCCTCGGGGTGGCGGACATCTCGCTGCTCGGCTGA
Translation:
MSAKDFLVELGTEELPPKALNSLGEAFLSGIEKGLKAAGLSYAAARFYAAPRRLAVLVEQLAVQQPDRTVNL
DGPPLQAAFDASGNPTQAALGFAKKCGVDLQQIDKSGPKLRFSQTIAGQPAAGLLPGIVEASLNELPIPKRM
RWAARREEFVRPTQWLVMLFGDDVVECEILAQKAGRESRGHRFHNPDNVRISSPAAYLEDLRGAHVLADFAE
RRELIAKRVAELAAEQQGSAIVPPSLLDEVTALVEWPVPLVCSFEERFLEVPQEALITTMQDNQKYFCLLDA
NGKLLPRFITVANVESKAPENIVSGNEKVVRPRLTDAEFFFKQDKKQPLESFNERLRNVVFQAQLGTVFEKA
QRVSGLAAYIAERIGGNAQNAARAGILSKCDLATEMVGEFPEMQGIAGYYYATHGGEAEDVALALNEQYMPR
GAGAELPSTLTGAAVAVADKLDTLVGIFGIGMLPTGSKDPYALRRAALGVLRILIEKQLDLDLVAAVNAAVE
QYGDKVKAAGLAEQVLDFVFDRLRARYEDEGVDVAVYQSVRALKPSSPLDFDQRVQAVQAFRQLPEAEALAA
ANKRVSNILAKSEDEVPPNVDASLLVEAAEKALGSAVANAESEVAPLAAARDYRAALARLAALREPVDTFFA
DVMVNVDDAAVRANRYALLAKLRGSFLGVADISLLG*
AnnotationID:365GeneID:356
AnnotatorUID: diggins
Modification Date/Time: 2005-03-22 11:11:10
Gene Name:glyS
Confidence Code:2
GeneProduct:glycyl-tRNA synthetase beta chain
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0751,pfam02092
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:6.1.1.14
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65694.1|AE016196_5 (AE016774) glycyl-tRNA synthetase, tetrameric type, beta subunit
           [Pseudomonas putida KT2440]
          Length = 684

 Score = 1065 bits (2755), Expect = 0.0
 Identities = 541/684 (79%), Positives = 606/684 (88%)

>gb|AAO53739.1| (AE016856) glycyl-tRNA synthetase, beta subunit [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 684

 Score = 1051 bits (2717), Expect = 0.0
 Identities = 535/684 (78%), Positives = 601/684 (87%)

>gb|AAO71343.1| (AE016847) glycine-tRNA synthetase, beta subunit [Salmonella
           enterica subsp. enterica serovar Typhi Ty2]
          Length = 689

 Score =  651 bits (1679), Expect = 0.0
 Identities = 357/688 (51%), Positives = 452/688 (65%), Gaps = 5/688 (0%)






 
Structural Features:
COG0751, GlyS, Glycyl-tRNA synthetase, beta subunit 
[Translation, ribosomal structure and biogenesis]
CD-Length = 691 residues,  99.9% aligned
Score =  888 bits (2297), Expect = 0.0
Identities = 369/690 (53%), Positives = 473/690 (68%), Gaps = 6/690 (0%)

pfam02092, tRNA_synt_2f, Glycyl-tRNA synthetase beta subunit.
CD-Length = 551 residues,  98.7% aligned
Score =  593 bits (1529), Expect = 3e-170
Identities = 264/544 (48%), Positives = 356/544 (65%), Gaps = 5/544 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:715




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17857




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33374




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:356_cdd.html


    		




 
URL:

















    ReferenceID:35528




  
Author/Investigator(s):
        Wagar EA, Giese MJ, Yasin B, Pang M.





  
Title:
        The glycyl-tRNA synthetase of Chlamydia trachomatis.




  
PubMed:
        7665503


MedLine:
        




    
Source:
J Bacteriol. 1995 Sep;177(17):5179-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7665503

















    ReferenceID:35531




  
Author/Investigator(s):
        Keng T, Webster TA, Sauer RT, Schimmel P.





  
Title:
        Gene for Escherichia coli glycyl-tRNA synthetase has tandem subunit coding regions in the same reading frame.




  
PubMed:
        6290471


MedLine:
        




    
Source:
J Biol Chem. 1982 Nov 10;257(21):12503-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6290471









           
Homologs By Global Alignment 

           

       



     
Gene ID:356
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
600
                           PA0008_tr
                           translation of PA0008
                           684
                           99.70
                           99.85
                           0
                           3399.0
     

     
     
28153
                           gb|AAG03398.1|AE004440_8
                           (AE004440) glycyl-tRNA synthetase beta chain [Pseudomonas aeruginosa PAO1]
                           684
                           99.70
                           99.85
                           0
                           3399.0
     

     
     
28154
                           gb|AAN65694.1|AE016196_5
                           (AE016774) glycyl-tRNA synthetase, tetrameric type, beta subunit [Pseudomonas putida KT2440]
                           684
                           79.09
                           88.59
                           0
                           2755.0
     

     
     
28155
                           gb|AAO53739.1|
                           (AE016856) glycyl-tRNA synthetase, beta subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           684
                           78.21
                           87.86
                           0
                           2717.0
     

     
     
28156
                           gb|AAP70370.1|
                           (AY236224) GlyS [uncultured bacterium]
                           693
                           53.96
                           67.96
                           10
                           1756.0
     

     






      

          
GC ORFID: 43358How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0008Glimmer Score: 

          
Start: 5813825Stop: 5811771

          
Length: 2055


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0008 translation of PA0008Homolog Bit Score 1313.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene4104 (ORF: BLASTX 1051.0), t_PA0008 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGCCAAGGATTTCCTGGTCGAACTGGGCACCGAAGAGCTGCCGCCAAAAGCCCTCAACAGCCTCGGC 
GAAGCCTTCCTCAGCGGTATCGAGAAGGGCCTCAAGGCTGCCGGTCTGAGTTACGCCGCTGCGCGTTTCTAT 
GCGGCGCCGCGCCGTCTCGCCGTGCTGGTCGAGCAACTCGCCGTGCAGCAGCCCGACCGTACCGTCAACCTA 
GACGGTCCGCCGCTACAGGCCGCCTTCGACGCCAGCGGTAACCCGACCCAGGCCGCCCTGGGCTTCGCCAAG 
AAGTGCGGCGTGGATCTACAGCAGATCGACAAGAGCGGTCCGAAGCTGCGCTTCAGCCAGACCATCGCCGGC 
CAACCGGCCGCCGGCCTGCTGCCGGGCATCGTCGAGGCCTCGTTGAACGAACTGCCGATTCCCAAGCGGATG 
CGCTGGGCAGCTCGCCGCGAGGAGTTCGTGCGTCCGACCCAATGGCTGGTGATGCTGTTCGGCGACGACGTG 
GTCGAGTGCGAGATCCTCGCCCAGAAAGCCGGGCGCGAATCCCGTGGCCACCGCTTCCACAATCCGGACAAT 
GTGCGTATTTCCAGCCCGGCAGCGTACCTGGAAGACCTGCGCGGCGCTCATGTGCTGGCCGATTTCGCCGAG 
CGCCGCGAGCTGATCGCCAAGCGCGTGGCAGAACTGGCCGCCGAGCAACAGGGCAGCGCCATCGTGCCGCCG 
AGCCTGCTCGACGAAGTGACTGCACTGGTCGAGTGGCCGGTGCCGCTGGTCTGCTCCTTCGAGGAGCGCTTC 
CTCGAGGTCCCGCAGGAAGCCCTGATCACCACCATGCAGGACAACCAGAAATACTTCTGCCTGCTGGACGCC 
AACGGCAAGCTGCTGCCGCGCTTCATCACCGTCGCCAACGTCGAGAGCAAGGCGCCGGAGAACATCGTCAGC 
GGTAACGAGAAGGTGGTTCGTCCGCGCCTCACCGACGCCGAGTTCTTCTTCAAGCAGGACAAGAAGCAGCCG 
CTCGAAAGCTTCAACGAGCGCCTGCGCAACGTAGTGTTCCAGGCCCAGCTCGGCACCGTGTTCGAGAAGGCC 
CAGCGCGTCTCCGGCCTGGCCGCCTACATCGCCGAACGTATAGGCGGCAATGCGCAGAACGCCGCGCGCGCC 
GGCATCCTGTCCAAGTGCGACCTGGCGACCGAGATGGTCGGCGAGTTCCCCGAGATGCAGGGCATCGCCGGC 
TACTACTACGCCACCCACGGCGGCGAAGCGGAAGACGTCGCCCTGGCCCTCAACGAGCAGTACATGCCGCGC 
GGCGCCGGCGCCGAGCTACCCTCGACCCTGACCGGCGCCGCCGTGGCGGTGGCCGACAAGCTCGATACCCTG 
GTCGGTATCTTCGGCATCGGCATGCTTCCCACCGGCAGCAAGGACCCCTACGCGCTGCGCCGCGCTGCGCTG 
GGCGTGCTGCGCATCCTCATCGAGAAGCAGCTCGACCTGGACCTGGTAGCCGCGGTCAACGCCGCCGTCGAG 
CAATATGGCGACAAGGTCAAGGCCGCCGGCCTGGCCGAGCAGGTGCTGGACTTCGTGTTCGACCGCCTGCGC 
GCGCGCTACGAGGACGAAGGCGTGGACGTGGCCGTGTACCAGTCGGTGCGCGCGCTCAAGCCAAGCTCGCCG 
CTGGACTTCGACCAGCGCGTACAGGCCGTCCAGGCCTTCCGCCAGTTGCCTGAAGCCGAGGCCCTGGCCGCG 
GCGAACAAACGGGTGTCGAATATTCTCGCCAAGTCCGAGGACGAGGTTCCGCCGAACGTGGATGCCAGCCTG 
CTGGTGGAAGCTGCCGAGAAGGCCCTGGGCAGCGCCGTGGCGAACGCCGAGAGCGAAGTCGCGCCGCTGGCA 
GCGGCACGCGACTATCGCGCCGCGCTGGCCCGCCTGGCGGCCTTGCGCGAGCCGGTGGATACGTTCTTCGCC 
GATGTGATGGTCAATGTCGACGACGCGGCGGTGCGCGCCAACCGCTATGCGCTGCTGGCCAAGCTGCGCGGG 
TCGTTCCTCGGGGTGGCGGACATCTCGCTGCTCGGCTGA