ViewGene
Locus Name:
GeneID:
Location:

GeneID:3551
TrivialName: PA14_64660
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_64660
PAO1 Ortholog Locus:PA4892
Sequence Length:672
Protein Length:223
Start: 5759121
Stop: 5759792
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08277.1|AE004902_5 (AE004902) urease accessory protein UreF [Pseudomonas aeruginosa PAO1]
Identities = 223/223 (100%), Positives = 223/223 (100%)
Sequence:
GTGAACAGCATCTGGGCGCGCCTGCGCCTGGCCAGCTCGCAGTTGCCGATCGGCGGATACAGCTATTCCCAG
GGCCTGGAGGCGGCCCTCGACAATGGCTGGGTGCGCGACGCCGAGAGCGCGCGGACCTGGCTGGTCGACCAG
TTGCAACTGAACCTGGCGCGCTTCGAGGCGCCGCTGCTGGCCGGCCTGCTGCGCGCCGCGCTGATCGGCGAC
TGGGCCGCCTGCGATGCGGCCAGCGAACGCCATAGGGCCAGCCGCGAGACCCGCGAGCTGGCCCAGGAGAGC
CGGCAGATGGGCTTTTCCCTGCACCAATTGCTGGAAGCCCTGCCCGAACTCGACGCTCCCGGCCGCGCCTGG
CTGGCGCGCCAGGACCCGCCCAATCTCGCCGCCGCCTGGGCGATGGCCGCGCGCGCCTGGCGCCTGGACGCC
GAGGAGGCGCTCAGTGCCTGGTTCTGGAGCTGGCTGGAAAACCAGTTGGCGGTGCTGATGAAAACCCTGCCG
CTCGGCCAACTGGCCGCGCAGAAACTTGCTTCCAGCCTGCTGCCGGAACTCGACCGCGCCTGCGCCGAGGCG
CTGCGGCGGCCCGCCGTCGAGGGCAGCGCCGCGTTCGGTCTGGCGCTGGCCAGCATGACCCACGAAACCCAG
TACAGCCGTCTGTTCCGTTCCTAG
Translation:
MNSIWARLRLASSQLPIGGYSYSQGLEAALDNGWVRDAESARTWLVDQLQLNLARFEAPLLAGLLRAALIGD
WAACDAASERHRASRETRELAQESRQMGFSLHQLLEALPELDAPGRAWLARQDPPNLAAAWAMAARAWRLDA
EEALSAWFWSWLENQLAVLMKTLPLGQLAAQKLASSLLPELDRACAEALRRPAVEGSAAFGLALASMTHETQ
YSRLFRS*
AnnotationID:3559GeneID:3551
AnnotatorUID: diggins
Modification Date/Time: 2005-03-11 12:12:00
Gene Name:ureF
Confidence Code:2
GeneProduct:urease accessory protein UreF
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0830,pfam01730
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58338.1| (AE016873) urease accessory protein UreF [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 224

 Score =  275 bits (703), Expect = 1e-73
 Identities = 145/224 (64%), Positives = 164/224 (73%), Gaps = 1/224 (0%)






 
Structural Features:
COG0830, UreF, Urease accessory protein UreF 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 229 residues,  98.7% aligned
Score =  183 bits (465), Expect = 2e-47
Identities = 96/228 (42%), Positives = 128/228 (56%), Gaps = 7/228 (3%)

pfam01730, UreF, UreF. This family consists of the Urease accessory protein UreF. 
CD-Length = 154 residues,  99.4% aligned
Score = 86.0 bits (213), Expect = 3e-18
Identities = 59/153 (38%), Positives = 76/153 (49%), Gaps = 3/153 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14877




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24247




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35164




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3551_cdd.html


    		




 
URL:

















    ReferenceID:35938




  
Author/Investigator(s):
        de Koning-Ward TF, Ward AC, Robins-Browne RM.





  
Title:
        Characterisation of the urease-encoding gene complex of Yersinia enterocolitica.




  
PubMed:
        8045421


MedLine:
        




    
Source:
Gene. 1994 Jul 22;145(1):25-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8045421

















    ReferenceID:35942




  
Author/Investigator(s):
        Mulrooney SB, Hausinger RP.





  
Title:
        Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation.




  
PubMed:
        2211515


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5837-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2211515

















    ReferenceID:35944




  
Author/Investigator(s):
        Jones BD, Mobley HL.





  
Title:
        Proteus mirabilis urease: nucleotide sequence determination and comparison with jack bean urease.




  
PubMed:
        2687233


MedLine:
        




    
Source:
J Bacteriol. 1989 Dec;171(12):6414-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2687233

















    ReferenceID:35952




  
Author/Investigator(s):
        Maeda M, Hidaka M, Nakamura A, Masaki H, Uozumi T.





  
Title:
        Cloning, sequencing, and expression of thermophilic Bacillus sp. strain TB-90 urease gene complex in Escherichia coli.




  
PubMed:
        8288539


MedLine:
        




    
Source:
J Bacteriol. 1994 Jan;176(2):432-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8288539

















    ReferenceID:35966




  
Author/Investigator(s):
        Zhu J, Teng CH, Chang CF, Chang CD, Simpson KW, Wei C, McDonough P, McDonough S, Chang YF.





  
Title:
        Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster.




  
PubMed:
        12652903


MedLine:
        




    
Source:
DNA Seq. 2002 Dec;13(6):321-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12652903









           
Homologs By Global Alignment 

           

       



     
Gene ID:3551
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5664
                           PA4892_tr
                           translation of PA4892
                           223
                           99.99
                           99.99
                           0
                           1139.0
     

     
     
157716
                           gb|AAG08277.1|AE004902_5
                           (AE004902) urease accessory protein UreF [Pseudomonas aeruginosa PAO1]
                           223
                           99.55
                           99.99
                           0
                           1136.0
     

     
     
157717
                           gb|AAO58338.1|
                           (AE016873) urease accessory protein UreF [Pseudomonas syringae pv. tomato str. DC3000]
                           226
                           64.60
                           73.45
                           5
                           704.0
     

     
     
157718
                           gb|AAL86693.1|AF417006_5
                           (AF417006) urease accessory protein [Nitrosococcus oceani]
                           224
                           59.37
                           68.30
                           1
                           629.0
     

     
     
157720
                           emb|CAE39136.1|
                           (BX640435) urease accessory protein [Bordetella parapertussis]
                           227
                           44.05
                           54.62
                           11
                           399.0
     

     






      

          
GC ORFID: 46553How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4892Glimmer Score: 

          
Start: 5022802Stop: 5023473

          
Length: 672


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4892 translation of PA4892Homolog Bit Score 443.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5823 (ORF: BLASTX 360.0), t_PA4892 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAACAGCATCTGGGCGCGCCTGCGCCTGGCCAGCTCGCAGTTGCCGATCGGCGGATACAGCTATTCCCAG 
GGCCTGGAGGCGGCCCTCGACAATGGCTGGGTGCGCGACGCCGAGAGCGCGCGGACCTGGCTGGTCGACCAG 
TTGCAACTGAACCTGGCGCGCTTCGAGGCGCCGCTGCTGGCCGGCCTGCTGCGCGCCGCGCTGATCGGCGAC 
TGGGCCGCCTGCGATGCGGCCAGCGAACGCCATAGGGCCAGCCGCGAGACCCGCGAGCTGGCCCAGGAGAGC 
CGGCAGATGGGCTTTTCCCTGCACCAATTGCTGGAAGCCCTGCCCGAACTCGACGCTCCCGGCCGCGCCTGG 
CTGGCGCGCCAGGACCCGCCCAATCTCGCCGCCGCCTGGGCGATGGCCGCGCGCGCCTGGCGCCTGGACGCC 
GAGGAGGCGCTCAGTGCCTGGTTCTGGAGCTGGCTGGAAAACCAGTTGGCGGTGCTGATGAAAACCCTGCCG 
CTCGGCCAACTGGCCGCGCAGAAACTTGCTTCCAGCCTGCTGCCGGAACTCGACCGCGCCTGCGCCGAGGCG 
CTGCGGCGGCCCGCCGTCGAGGGCAGCGCCGCGTTCGGTCTGGCGCTGGCCAGCATGACCCACGAAACCCAG 
TACAGCCGTCTGTTCCGTTCCTAG