ViewGene
Locus Name:
GeneID:
Location:

GeneID:354
TrivialName: PA14_67510
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67510
PAO1 Ortholog Locus:PA5112
Sequence Length:1941
Protein Length:646
Start: 6028491
Stop: 6026551
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08497.1|AE004924_2 (AE004924) esterase EstA [Pseudomonas aeruginosa PAO1]
Identities = 646/646 (100%), Positives = 646/646 (100%)
Sequence:
ATGATCAGAATGGCGCTCAAGCCACTGGTAGCGGCCTGCCTGCTGGCTTCGCTGTCCACCGCCCCGCAGGCT
GCTCCTTCGCCCTATTCGACGCTGGTGGTGTTCGGCGACAGCCTCAGCGATGCCGGGCAGTTCCCCGATCCT
GCCGGCCCCGCCGGAAGCACCTCGCGTTTCACCAACCGGGTCGGCCCGACCTACCAGAACGGCAGCGGCGAG
ATCTTCGGACCGACCGCGCCCATGCTGCTCGGCAATCAGCTCGGCATCGCCCCGGGCGACCTGGCTGCCTCG
ACCTCGCCGGTCAACGCCCAGCAGGGCATCGCCGACGGCAACAACTGGGCGGTGGGCGGCTACCGGACCGAT
CAGATCTACGACTCGATCACCGCGGCCAACGGCTCGCTGATCGAGCGCGACAATACCCTCCTGCGCAGCCGC
GATGGCTACCTGGTGGACCGTGCCCGCCAGGGCCTGGGTGCCGACCCGAACGCGTTGTACTACATCACCGGC
GGCGGCAACGACTTCCTCCAGGGGCGCATCCTCAACGACGTCCAGGCACAACAGGCCGCCGGTCGCCTGGTG
GATAGCGTGCAGGCCCTGCAGCAGGCCGGCGCGCGCTACATCGTGGTCTGGCTGTTGCCCGACCTGGGCCTG
ACCCCGGCTACCTTCGGTGGTCCCTTGCAGCCTTTCGCCAGCCAACTCAGCGGCACGTTCAACGCCGAGCTG
ACCGCCCAGTTGAGCCAGGCCGGCGCCAACGTCATTCCGTTGAACATCCCGCTGCTGCTCAAGGAAGGCATG
GCCAACCCGGCTTCCTTCGGCCTGGCCGCCGACCAGAACCTGATCGGCACCTGTTTCAGCGGCAACGGCTGC
ACCATGAACCCGACCTACGGGATCAACGGCAGCACGCCCGACCCGAGCAAATTGCTGTTCAACGACAGCGTG
CACCCGACCATCACCGGCCAGCGCCTGATCGCCGACTACACCTACTCGCTGCTGTCGGCGCCCTGGGAGCTG
ACCCTGCTGCCGGAAATGGCCCACGGCACCCTGCGTGCCTACCAGGACGAACTGCGCAGCCAGTGGCAGGCG
GACTGGGAGAACTGGCAGAACGTCGGCCAGTGGCGCGGCTTCGTCGGCGGCGGTGGCCAGCGCCTGGACTTC
GACTCCCAGGACAGCGCCGCCAGCGGCGACGGCAATGGCTACAACCTGACCCTTGGTGGCAGCTACCGCATC
GACGAGGCCTGGCGCGCCGGGGTCGCCGCCGGTTTCTACCGGCAGAAGTTGGAAGCCGGCGCCAAGGATTCC
GACTACCGGATGAACAGCTACATGGCCAGCGCCTTCGTGCAGTACCAGGAAAACCGCTGGTGGGCTGACGCG
GCGCTGACCGGCGGCTACCTCGACTACGACGACCTGAAGCGCAAGTTCGCCCTGGGCGGCGGCGAGCGCAGC
GAGAAAGGCGACACCAACGGCCATCTGTGGGCGTTCAGCGCGCGCCTGGGCTACGACATCGCCCAGCAGGCC
GACAGTCCCTGGCACCTGTCGCCGTTCGTCAGCGCCGACTACGCGCGGGTCGAGGTCGACGGCTATTCCGAG
AAGGGCGCCAGCGCCACCGCGCTCGACTACGATGACCAGAAGCGCAGCTCGAAGCGCCTGGGCGCCGGCCTG
CAAGGCAAGTACGCGTTCGGCAGCGATACCCAGCTGTTCGCCGAGTACGCCCACGAACGCGAGTACGAGGAC
GACACCCAGGACCTGACCATGTCCCTCAACAGCCTGCCGGGCAATCGCTTCACCCTCGAAGGCTACACCCCG
CAGGACCACCTCAACCGCGTCTCCCTCGGCTTCAGCCAGAAGCTGGCGCCGGAGCTGTCGCTGCGCGGCGGC
TACAACTGGCGCAAGGGCGAGGACGATACCCAGCAGAGCGTCAGCCTGGCGCTGAGCCTGGACTTCTGA
Translation:
MIRMALKPLVAACLLASLSTAPQAAPSPYSTLVVFGDSLSDAGQFPDPAGPAGSTSRFTNRVGPTYQNGSGE
IFGPTAPMLLGNQLGIAPGDLAASTSPVNAQQGIADGNNWAVGGYRTDQIYDSITAANGSLIERDNTLLRSR
DGYLVDRARQGLGADPNALYYITGGGNDFLQGRILNDVQAQQAAGRLVDSVQALQQAGARYIVVWLLPDLGL
TPATFGGPLQPFASQLSGTFNAELTAQLSQAGANVIPLNIPLLLKEGMANPASFGLAADQNLIGTCFSGNGC
TMNPTYGINGSTPDPSKLLFNDSVHPTITGQRLIADYTYSLLSAPWELTLLPEMAHGTLRAYQDELRSQWQA
DWENWQNVGQWRGFVGGGGQRLDFDSQDSAASGDGNGYNLTLGGSYRIDEAWRAGVAAGFYRQKLEAGAKDS
DYRMNSYMASAFVQYQENRWWADAALTGGYLDYDDLKRKFALGGGERSEKGDTNGHLWAFSARLGYDIAQQA
DSPWHLSPFVSADYARVEVDGYSEKGASATALDYDDQKRSSKRLGAGLQGKYAFGSDTQLFAEYAHEREYED
DTQDLTMSLNSLPGNRFTLEGYTPQDHLNRVSLGFSQKLAPELSLRGGYNWRKGEDDTQQSVSLALSLDF*
AnnotationID:363GeneID:354
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 12:12:25
Gene Name:estA
Confidence Code:1
GeneProduct:esterase EstA
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:autotransporting lipolytic enzyme
Functional Category Confidence Code:5
COGs:COG3240,pfam03797
Secondary Functional Category(ies):Secreted Factors (toxins, enzymes, alginate)
EC Number:3.1.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL07272.1| (AY055203) autotransporting lipolytic enzyme [Pseudomonas sp.
           HSM0414]
          Length = 636

 Score =  875 bits (2262), Expect = 0.0
 Identities = 425/646 (65%), Positives = 492/646 (76%), Gaps = 10/646 (1%)

>dbj|BAC21259.1| (AB093583) esterase [Pseudomonas sp. B11-1]
          Length = 637

 Score =  848 bits (2191), Expect = 0.0
 Identities = 415/647 (64%), Positives = 488/647 (75%), Gaps = 11/647 (1%)

>gb|AAO54111.1| (AE016857) autotransporting lipase, GDSL family [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 640

 Score =  810 bits (2091), Expect = 0.0
 Identities = 398/636 (62%), Positives = 478/636 (75%), Gaps = 6/636 (0%)






 
Structural Features:
COG3240, COG3240, Phospholipase/lecithinase/hemolysin [Lipid metabolism]
CD-Length = 370 residues, 100.0% aligned
Score =  348 bits (894), Expect = 1e-96
Identities = 148/380 (38%), Positives = 198/380 (52%), Gaps = 17/380 (4%)

pfam03797, Autotransporter, Autotransporter beta-domain.
CD-Length = 287 residues,  97.2% aligned
Score =  111 bits (277), Expect = 3e-25
Identities = 66/285 (23%), Positives = 101/285 (35%), Gaps = 7/285 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:711




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17853




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34956




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:354_cdd.html


    		




 
URL:

















    ReferenceID:35804




  
Author/Investigator(s):
        Wilhelm S, Tommassen J, Jaeger KE.





  
Title:
        A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa.




  
PubMed:
        10559163


MedLine:
        




    
Source:
J Bacteriol. 1999 Nov;181(22):6977-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10559163









           
Homologs By Global Alignment 

           

       



     
Gene ID:354
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
598
                           PA5112_tr
                           translation of PA5112
                           646
                           99.99
                           99.99
                           0
                           3397.0
     

     
     
28142
                           gb|AAG08497.1|AE004924_2
                           (AE004924) esterase EstA [Pseudomonas aeruginosa PAO1]
                           646
                           99.99
                           99.99
                           0
                           3397.0
     

     
     
28144
                           gb|AAB61674.1|
                           (AF005091) lipase/esterase; EstA [Pseudomonas aeruginosa]
                           646
                           99.99
                           99.99
                           0
                           3397.0
     

     
     
28143
                           emb|CAC14200.1|
                           (AJ277638) PapA protein [Pseudomonas aeruginosa]
                           663
                           97.43
                           97.43
                           17
                           3397.0
     

     
     
28145
                           gb|AAL07272.1|
                           (AY055203) autotransporting lipolytic enzyme [Pseudomonas sp. HSM0414]
                           648
                           65.74
                           76.23
                           14
                           2272.0
     

     






      

          
GC ORFID: 43356How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5112Glimmer Score: 

          
Start: 5292176Stop: 5290236

          
Length: 1941


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5112 translation of PA5112Homolog Bit Score 1313.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1438 (ORF: BLASTX 812.0), t_PA5112 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCAGAATGGCGCTCAAGCCACTGGTAGCGGCCTGCCTGCTGGCTTCGCTGTCCACCGCCCCGCAGGCT 
GCTCCTTCGCCCTATTCGACGCTGGTGGTGTTCGGCGACAGCCTCAGCGATGCCGGGCAGTTCCCCGATCCT 
GCCGGCCCCGCCGGAAGCACCTCGCGTTTCACCAACCGGGTCGGCCCGACCTACCAGAACGGCAGCGGCGAG 
ATCTTCGGACCGACCGCGCCCATGCTGCTCGGCAATCAGCTCGGCATCGCCCCGGGCGACCTGGCTGCCTCG 
ACCTCGCCGGTCAACGCCCAGCAGGGCATCGCCGACGGCAACAACTGGGCGGTGGGCGGCTACCGGACCGAT 
CAGATCTACGACTCGATCACCGCGGCCAACGGCTCGCTGATCGAGCGCGACAATACCCTCCTGCGCAGCCGC 
GATGGCTACCTGGTGGACCGTGCCCGCCAGGGCCTGGGTGCCGACCCGAACGCGTTGTACTACATCACCGGC 
GGCGGCAACGACTTCCTCCAGGGGCGCATCCTCAACGACGTCCAGGCACAACAGGCCGCCGGTCGCCTGGTG 
GATAGCGTGCAGGCCCTGCAGCAGGCCGGCGCGCGCTACATCGTGGTCTGGCTGTTGCCCGACCTGGGCCTG 
ACCCCGGCTACCTTCGGTGGTCCCTTGCAGCCTTTCGCCAGCCAACTCAGCGGCACGTTCAACGCCGAGCTG 
ACCGCCCAGTTGAGCCAGGCCGGCGCCAACGTCATTCCGTTGAACATCCCGCTGCTGCTCAAGGAAGGCATG 
GCCAACCCGGCTTCCTTCGGCCTGGCCGCCGACCAGAACCTGATCGGCACCTGTTTCAGCGGCAACGGCTGC 
ACCATGAACCCGACCTACGGGATCAACGGCAGCACGCCCGACCCGAGCAAATTGCTGTTCAACGACAGCGTG 
CACCCGACCATCACCGGCCAGCGCCTGATCGCCGACTACACCTACTCGCTGCTGTCGGCGCCCTGGGAGCTG 
ACCCTGCTGCCGGAAATGGCCCACGGCACCCTGCGTGCCTACCAGGACGAACTGCGCAGCCAGTGGCAGGCG 
GACTGGGAGAACTGGCAGAACGTCGGCCAGTGGCGCGGCTTCGTCGGCGGCGGTGGCCAGCGCCTGGACTTC 
GACTCCCAGGACAGCGCCGCCAGCGGCGACGGCAATGGCTACAACCTGACCCTTGGTGGCAGCTACCGCATC 
GACGAGGCCTGGCGCGCCGGGGTCGCCGCCGGTTTCTACCGGCAGAAGTTGGAAGCCGGCGCCAAGGATTCC 
GACTACCGGATGAACAGCTACATGGCCAGCGCCTTCGTGCAGTACCAGGAAAACCGCTGGTGGGCTGACGCG 
GCGCTGACCGGCGGCTACCTCGACTACGACGACCTGAAGCGCAAGTTCGCCCTGGGCGGCGGCGAGCGCAGC 
GAGAAAGGCGACACCAACGGCCATCTGTGGGCGTTCAGCGCGCGCCTGGGCTACGACATCGCCCAGCAGGCC 
GACAGTCCCTGGCACCTGTCGCCGTTCGTCAGCGCCGACTACGCGCGGGTCGAGGTCGACGGCTATTCCGAG 
AAGGGCGCCAGCGCCACCGCGCTCGACTACGATGACCAGAAGCGCAGCTCGAAGCGCCTGGGCGCCGGCCTG 
CAAGGCAAGTACGCGTTCGGCAGCGATACCCAGCTGTTCGCCGAGTACGCCCACGAACGCGAGTACGAGGAC 
GACACCCAGGACCTGACCATGTCCCTCAACAGCCTGCCGGGCAATCGCTTCACCCTCGAAGGCTACACCCCG 
CAGGACCACCTCAACCGCGTCTCCCTCGGCTTCAGCCAGAAGCTGGCGCCGGAGCTGTCGCTGCGCGGCGGC 
TACAACTGGCGCAAGGGCGAGGACGATACCCAGCAGAGCGTCAGCCTGGCGCTGAGCCTGGACTTCTGA