ViewGene
Locus Name:
GeneID:
Location:

GeneID:3532
TrivialName: PA14_13830
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_13830
PAO1 Ortholog Locus:PA3872
Sequence Length:684
Protein Length:227
Start: 1189694
Stop: 1190377
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07259.1|AE004804_7 (AE004804) respiratory nitrate reductase gamma chain [Pseudomonas aeruginosa PAO1]
Identities = 226/227 (99%), Positives = 227/227 (100%)
Sequence:
ATGTCGACCAATCTTCTGTTCTTCGGGATCTATCCCTATGTCGCGCTGCTGATCTGCCTGGTCGGCAGCTGG
GCGCGCTTCGACCTCTCGCAGTACACCTGGAAGGCCGGTTCCAGCCAGATGCTCAGCAAGAAGGGCATGCGG
GTATACAGCAACCTGTTCCACGTCGGCGTGCTGTTCATCCTCGCCGGCCACTTCGTCGGCCTGCTGACCCCG
GCCTCGGTCTACCACCACCTGATCAGCACCGAGAACAAGCAACTGCTGGCGATGGTCTCCGGCGGCTTCTTC
GGCGTGCTCTGCTTCATCGGCCTGAGCGGACTGATCCTGCGCCGCCTGACCGACGCGCGGGTGCGCGCCACC
GGCAACGCCTCTGACCTGATGATCCTGCTGGTGCTCTACGCCCAGCTGATCCTCGGCCTCTCCACCATCGTC
GCCTCGACCCATCACATGGATGGCTCGGTGATGGTGATGCTCGCCGACTGGGCCCAGGCCATCGTCACCCTG
CGTCCGCTGGCGGCGGCCGAAGCCATCGCGCCGGTGGGCCTGGTCTACAAGCTGCACGTCGGCCTGGGCCTG
ACCCTGTTCGTGCTGTTCCCCTTCACCCGCCTGGTGCACATCGTCAGCGCGCCGGTGTGGTACCTGGGCCGG
CGCTACCAGATCGTGCGTCAGAAACGTCCTGCCTGA
Translation:
MSTNLLFFGIYPYVALLICLVGSWARFDLSQYTWKAGSSQMLSKKGMRVYSNLFHVGVLFILAGHFVGLLTP
ASVYHHLISTENKQLLAMVSGGFFGVLCFIGLSGLILRRLTDARVRATGNASDLMILLVLYAQLILGLSTIV
ASTHHMDGSVMVMLADWAQAIVTLRPLAAAEAIAPVGLVYKLHVGLGLTLFVLFPFTRLVHIVSAPVWYLGR
RYQIVRQKRPA*
AnnotationID:3540GeneID:3532
AnnotatorUID: diggins
Modification Date/Time: 2005-04-25 09:09:00
Gene Name:narI
Confidence Code:2
GeneProduct:respiratory nitrate reductase gamma chain
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2181,pfam02665
Secondary Functional Category(ies):
EC Number:1.7.99.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAG34376.1|AF197465_8 (AF197465) respiratory nitrate reductase gamma subunit; NarI
           [Pseudomonas fluorescens]
          Length = 226

 Score =  365 bits (936), Expect = e-100
 Identities = 181/221 (81%), Positives = 199/221 (90%)

>gb|AAC74547.1| (AE000243) cryptic nitrate reductase 2, gamma subunit [Escherichia
           coli K12]
          Length = 226

 Score =  233 bits (595), Expect = 5e-61
 Identities = 116/222 (52%), Positives = 154/222 (69%), Gaps = 1/222 (0%)








 
Structural Features:
COG2181, NarI, Nitrate reductase gamma subunit 
[Energy production and conversion]
CD-Length = 228 residues,  98.7% aligned
Score =  229 bits (586), Expect = 4e-61
Identities = 126/226 (55%), Positives = 157/226 (69%), Gaps = 3/226 (1%)

pfam02665, Nitrate_red_gam, Nitrate reductase gamma subunit.
CD-Length = 223 residues,  99.1% aligned
Score =  176 bits (447), Expect = 5e-45
Identities = 93/223 (41%), Positives = 129/223 (57%), Gaps = 3/223 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7067




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24209




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31692




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3532_cdd.html


    		




 
URL:

















    ReferenceID:34324




  
Author/Investigator(s):
        Sodergren EJ, DeMoss JA.





  
Title:
        narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.




  
PubMed:
        2832376


MedLine:
        




    
Source:
J Bacteriol. 1988 Apr;170(4):1721-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2832376

















    ReferenceID:34325




  
Author/Investigator(s):
        Blasco F, Iobbi C, Giordano G, Chippaux M, Bonnefoy V.





  
Title:
        Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer.




  
PubMed:
        2674654


MedLine:
        




    
Source:
Mol Gen Genet. 1989 Aug;218(2):249-56.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2674654

















    ReferenceID:34326




  
Author/Investigator(s):
        Berks BC, Page MD, Richardson DJ, Reilly A, Cavill A, Outen F, Ferguson SJ.





  
Title:
        Sequence analysis of subunits of the membrane-bound nitrate reductase from a denitrifying bacterium: the integral membrane subunit provides a prototype for the dihaem electron-carrying arm of a redox loop.




  
PubMed:
        7746153


MedLine:
        




    
Source:
Mol Microbiol. 1995 Jan;15(2):319-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7746153

















    ReferenceID:34327




  
Author/Investigator(s):
        Sodergren EJ, Hsu PY, DeMoss JA.





  
Title:
        Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli.




  
PubMed:
        3053688


MedLine:
        




    
Source:
J Biol Chem. 1988 Nov 5;263(31):16156-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3053688









           
Homologs By Global Alignment 

           

       



     
Gene ID:3532
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5635
                           PA3872_tr
                           translation of PA3872
                           227
                           99.55
                           99.99
                           0
                           1148.0
     

     
     
157091
                           gb|AAG07259.1|AE004804_7
                           (AE004804) respiratory nitrate reductase gamma chain [Pseudomonas aeruginosa PAO1]
                           227
                           99.55
                           99.99
                           0
                           1148.0
     

     
     
157092
                           emb|CAA75543.1|
                           (Y15252) respiratory nitrate reductase gamma subunit [Pseudomonas aeruginosa]
                           227
                           99.55
                           99.99
                           0
                           1148.0
     

     
     
157093
                           gb|AAG34376.1|AF197465_8
                           (AF197465) respiratory nitrate reductase gamma subunit; NarI [Pseudomonas fluorescens]
                           228
                           80.26
                           88.15
                           3
                           931.0
     

     
     
157103
                           dbj|BAB35155.1|
                           (AP002556) nitrate reductase 1 gamma subunit [Escherichia coli O157:H7]
                           228
                           50.87
                           66.66
                           4
                           583.0
     

     






      

          
GC ORFID: 46534How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3872Glimmer Score: 

          
Start: 3764244Stop: 3763561

          
Length: 684


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3872 translation of PA3872Homolog Bit Score 446.0

          
Other Homologs:  t_PA3872 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGACCAATCTTCTGTTCTTCGGGATCTATCCCTATGTCGCGCTGCTGATCTGCCTGGTCGGCAGCTGG 
GCGCGCTTCGACCTCTCGCAGTACACCTGGAAGGCCGGTTCCAGCCAGATGCTCAGCAAGAAGGGCATGCGG 
GTATACAGCAACCTGTTCCACGTCGGCGTGCTGTTCATCCTCGCCGGCCACTTCGTCGGCCTGCTGACCCCG 
GCCTCGGTCTACCACCACCTGATCAGCACCGAGAACAAGCAACTGCTGGCGATGGTCTCCGGCGGCTTCTTC 
GGCGTGCTCTGCTTCATCGGCCTGAGCGGACTGATCCTGCGCCGCCTGACCGACGCGCGGGTGCGCGCCACC 
GGCAACGCCTCTGACCTGATGATCCTGCTGGTGCTCTACGCCCAGCTGATCCTCGGCCTCTCCACCATCGTC 
GCCTCGACCCATCACATGGATGGCTCGGTGATGGTGATGCTCGCCGACTGGGCCCAGGCCATCGTCACCCTG 
CGTCCGCTGGCGGCGGCCGAAGCCATCGCGCCGGTGGGCCTGGTCTACAAGCTGCACGTCGGCCTGGGCCTG 
ACCCTGTTCGTGCTGTTCCCCTTCACCCGCCTGGTGCACATCGTCAGCGCGCCGGTGTGGTACCTGGGCCGG 
CGCTACCAGATCGTGCGTCAGAAACGTCCTGCCTGA