ViewGene
Locus Name:
GeneID:
Location:

GeneID:3522
TrivialName: PA14_04150
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_04150
PAO1 Ortholog Locus:PA0318
Sequence Length:666
Protein Length:221
Start: 372315
Stop: 372980
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03707.1|AE004470_1 (AE004470) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 221/221 (100%), Positives = 221/221 (100%)
Sequence:
ATGAGTTACCAGCATCAATATATCGACGGCACCCCGATCCACTTCCCCCTGGGCAAGCTGGTCTGCGTCGGG
CGCAACTACGCCGAACACGCCAAGGAACTGAACAACCCGGTTCCCACCGAGCCGCTGCTGTTCATCAAGCCC
GGCTCCTGCGTGGTGCCGGTGAGCGGCGGGCTGAGCCTGCCGCAGGAGCGCGGCTCGGTACACTACGAGGCG
GAAATCGCCGTGCTGATCGGCAAGCCGCTGTCGCGCAAGCCGAACCGCGAGGAGGTCCTCGACGCGATCTCC
GGCTATGCGCCGGCGCTGGACCTGACCTTGCGCGAGGTCCAGGCGAAACTGAAGGAAAAGGGCCTGCCCTGG
GAGCTGGCAAAGAGCTTCGATGGCGCCGGCGTGCTGTCGCCGTTCGTGAACGCCGACCAGTTCCCCGACCCG
ACCGACATCGGCATCCGCCTGACCATCAACGGCGAGGTGCGCCAGGACGGCAACAGCCGCGACATGCTCAAT
CCGATCGTGCCGCTGATCCAGCACATCGCCGGGCATTTCTCGCTACAGGCCGGCGATGTGGTGCTGACCGGC
ACGCCGTCCGGGGTCGGGCCGCTGAACAGCGGCGACGAGTTGCTGCTCGAATTGCCGGGCGCCAGCCGTTTC
GAGACCCGCGTGCTCTGA
Translation:
MSYQHQYIDGTPIHFPLGKLVCVGRNYAEHAKELNNPVPTEPLLFIKPGSCVVPVSGGLSLPQERGSVHYEA
EIAVLIGKPLSRKPNREEVLDAISGYAPALDLTLREVQAKLKEKGLPWELAKSFDGAGVLSPFVNADQFPDP
TDIGIRLTINGEVRQDGNSRDMLNPIVPLIQHIAGHFSLQAGDVVLTGTPSGVGPLNSGDELLLELPGASRF
ETRVL*
AnnotationID:3530GeneID:3522
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 12:12:23
Gene Name:
Confidence Code:3
GeneProduct:putative fumarylacetoacetate hydrolase family protein
Cell Localization:()
Synonyms:ycgM
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0179,pfam01557
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70718.1|AE016715_7 (AE016793) fumarylacetoacetate hydrolase family protein
           [Pseudomonas putida KT2440]
          Length = 221

 Score =  372 bits (954), Expect = e-102
 Identities = 178/221 (80%), Positives = 197/221 (89%)

>gb|AAO58718.1| (AE016875) fumarylacetoacetate hydrolase family protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 221

 Score =  339 bits (870), Expect = 6e-93
 Identities = 161/220 (73%), Positives = 191/220 (86%)








 
Structural Features:
COG0179, MhpD, 2-keto-4-pentenoate hydratase/
2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 266 residues,  83.1% aligned
Score =  228 bits (582), Expect = 5e-61
Identities = 88/224 (39%), Positives = 132/224 (58%), Gaps = 6/224 (2%)

pfam01557, FAA_hydrolase, Fumarylacetoacetate (FAA) hydrolase family. 
CD-Length = 168 residues, 100.0% aligned
Score =  154 bits (392), Expect = 6e-39
Identities = 67/170 (39%), Positives = 97/170 (57%), Gaps = 3/170 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7047




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24189




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33663




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3522_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3522
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5616
                           PA0318_tr
                           translation of PA0318
                           221
                           99.99
                           99.99
                           0
                           1150.0
     

     
     
156443
                           gb|AAG03707.1|AE004470_1
                           (AE004470) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           221
                           99.99
                           99.99
                           0
                           1150.0
     

     
     
156444
                           gb|AAN70718.1|AE016715_7
                           (AE016793) fumarylacetoacetate hydrolase family protein [Pseudomonas putida KT2440]
                           221
                           80.54
                           89.14
                           0
                           954.0
     

     
     
156445
                           gb|AAO58718.1|
                           (AE016875) fumarylacetoacetate hydrolase family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           221
                           72.85
                           86.42
                           0
                           868.0
     

     
     
156454
                           gb|AAG56031.1|AE005335_4
                           (AE005335) putative isomerase [Escherichia coli O157:H7 EDL933]
                           222
                           51.35
                           68.46
                           4
                           554.0
     

     






      

          
GC ORFID: 46524How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0318Glimmer Score: 

          
Start: 6173657Stop: 6174322

          
Length: 666


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0318 translation of PA0318Homolog Bit Score 447.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6525 (ORF: BLASTX 339.0), t_PA0318 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGTTACCAGCATCAATATATCGACGGCACCCCGATCCACTTCCCCCTGGGCAAGCTGGTCTGCGTCGGG 
CGCAACTACGCCGAACACGCCAAGGAACTGAACAACCCGGTTCCCACCGAGCCGCTGCTGTTCATCAAGCCC 
GGCTCCTGCGTGGTGCCGGTGAGCGGCGGGCTGAGCCTGCCGCAGGAGCGCGGCTCGGTACACTACGAGGCG 
GAAATCGCCGTGCTGATCGGCAAGCCGCTGTCGCGCAAGCCGAACCGCGAGGAGGTCCTCGACGCGATCTCC 
GGCTATGCGCCGGCGCTGGACCTGACCTTGCGCGAGGTCCAGGCGAAACTGAAGGAAAAGGGCCTGCCCTGG 
GAGCTGGCAAAGAGCTTCGATGGCGCCGGCGTGCTGTCGCCGTTCGTGAACGCCGACCAGTTCCCCGACCCG 
ACCGACATCGGCATCCGCCTGACCATCAACGGCGAGGTGCGCCAGGACGGCAACAGCCGCGACATGCTCAAT 
CCGATCGTGCCGCTGATCCAGCACATCGCCGGGCATTTCTCGCTACAGGCCGGCGATGTGGTGCTGACCGGC 
ACGCCGTCCGGGGTCGGGCCGCTGAACAGCGGCGACGAGTTGCTGCTCGAATTGCCGGGCGCCAGCCGTTTC 
GAGACCCGCGTGCTCTGA