ViewGene
Locus Name:
GeneID:
Location:

GeneID:352
TrivialName: PA14_50360
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_50360
PAO1 Ortholog Locus:PA1086
Sequence Length:2052
Protein Length:683
Start: 4477765
Stop: 4475714
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04475.1|AE004539_17 (AE004539) flagellar hook-associated protein 1 FlgK [Pseudomonas aeruginosa PAO1]
Identities = 676/683 (98%), Positives = 681/683 (99%)
Sequence:
ATGTCCGACCTACTCTCGATAGGCCTGTCCGGCCTCGGCACCAGCCAGACCTGGCTGACCATCACCGGGCAC
AACATCACCAACGTCAAGACGCCGGGCTACAGCCGCCAGGATGCCATCCAGCAGACCCAGGTCCCGCAGTTC
AGCGGTGCCGGCTACATGGGCTCGGGCAGCCAGATCGTCGACGTGCGCCGCCTGGCCAGTGACTTCCTCACC
GGCCAGTTGCGCAACGCCACCAGCCAGAACAGCGAGTTGAGCGCTTTCCTGGGCCAGATCGACCAGCTCAAC
TCGCTGCTGGCGGATAACACCACCGGGGTCAGCCCGGCGATGCAGCGTTTCTTTTCCGCCTTGCAGACCGCT
GCGCAGAACCCGTCCTCGACCGAGGCGCGCGAGGCGGTGCTGGCCCAGGCGCAGGGCCTGAGCAAGACCTTC
AATACCCTCTACGACCAACTGGACAAGCAGAACAGCCTGATCAACCAGCAGTTGGGTGCCCTGACCTCGCAG
GTGAACAATCTCAGCCAGAGCGTGGCCGAGTACAACGATGCCATCGCCAAGGCGAAATCCGCCGGGGCCGTG
CCCAACGATCTGCTGGACGCGCGCGACGAAGCGGTGCGCAAGCTCTCCGAGATGGTCGGCGTGACCGCGGTC
ACCCAGGACGACAACAGCGTCAGCCTGTTCATCGGCAGCGGCCAGCCGCTGGTGGTGGGCAATACCGTTTCG
ACGCTGAGCGTGGTGCCGGGGCTGGACGACCCGACCCGCTACCAGGTGCAGCTCACGCTTGGCGACTCCACG
CAAAACGTGACCCGGTTGGTCTCCGGCGGGCAGATGGGCGGCCTGCTGGCGTATCGCGATACCGTCCTGGAT
AGCTCCTACAACAAGCTCGGCCAACTTGCGCTGACCTTCGCCGATACCGTCAACAAGCAACTGGGGCAGGGC
CTGGACCTGGCGGGCAAGGCCGGCGCCAACCTGTTCGGCGACATCAACGATCCGGACATCACGGCGCTGCGG
GTCCTGGCGAAGAACGGCAACACCGGCAATGTCCACGCCAACCTGAACATCACCGATACCAGCAAGCTGAAT
TCCAGCGATTTCCGCCTGGACTTCGACGGCACCAATTTCACCGCGCGGCGCCTGGGCGACGACGCAAGCATG
CAGGTCACGGTCAGCGGTACCGGTCCCTACACCCTCTCGTTCAAGGATGCCAACGGCGTCGACCAGGGCTTC
AGCGTGACCCTCGACCAACTGCCGGCCGCGGGCGACCGCTTCACCCTGCAGCCGACCCGGCGCGGCGCCAGC
GATATCGAGACAACCCTGAAGAACGCTTCGCAACTGGCTTTCGCCGGGTCCGCTCGCGCCGAGGCAACCACC
AACAACCGCGGCAGCGGCGCCATCGGCCAGCCGAACCTGGTCGGCGGACCGTCGCCGATCGATCCGGCGGTT
CTGCAGAACGCCTTCGGCGCCAATGGTCTGCCGCTGAGCGCGACGGTCAGCGCGGATGGCAAGACCTACACC
ATGACCTCGCCGCTGCCGGCCGGTTGGAGTTATGTCGACAAGGACGGCAACGCGCTGCCCGGCAGTCCGACG
CTGAACAGCGGTACCAGCAACAGCGTGCGCATGGCCTATACCGATCCGGGCAGCGGCCAGACCTATACCTAC
GAGTTCAACCTGTCCAACGTGCCGCAGACTGGCGACAGTTTCACCCTGAGCTTCAACAAGGACGGCATTTCG
GACAACCGCAACGCCCTCAACCTGAATGCCTTGCAGACCAAGCCGACGGTGGGCGGTACCGGCAGCACGGGG
TCTACCTACAACGACGCCTACGGTGGACTGGTGGAGCGGGTCGGCACCCTGACCGCCCAGGCGCGCGCCAGC
GCCGACGCCTCGCAGACCGTGCTCAAGCAGGCGCAGGACAGCCGCGACTCGCTGTCCGGGGTGAGCCTCGAC
GAAGAGGCGGCCAACCTGATCCAGTTCCAGCAGTACTACAGCGCGTCGGCCCAGGTCATTCAAGTTGCCCGC
TCGCTGTTCGATACCCTGATAGGCGCGTTCCGCTGA
Translation:
MSDLLSIGLSGLGTSQTWLTITGHNITNVKTPGYSRQDAIQQTQVPQFSGAGYMGSGSQIVDVRRLASDFLT
GQLRNATSQNSELSAFLGQIDQLNSLLADNTTGVSPAMQRFFSALQTAAQNPSSTEAREAVLAQAQGLSKTF
NTLYDQLDKQNSLINQQLGALTSQVNNLSQSVAEYNDAIAKAKSAGAVPNDLLDARDEAVRKLSEMVGVTAV
TQDDNSVSLFIGSGQPLVVGNTVSTLSVVPGLDDPTRYQVQLTLGDSTQNVTRLVSGGQMGGLLAYRDTVLD
SSYNKLGQLALTFADTVNKQLGQGLDLAGKAGANLFGDINDPDITALRVLAKNGNTGNVHANLNITDTSKLN
SSDFRLDFDGTNFTARRLGDDASMQVTVSGTGPYTLSFKDANGVDQGFSVTLDQLPAAGDRFTLQPTRRGAS
DIETTLKNASQLAFAGSARAEATTNNRGSGAIGQPNLVGGPSPIDPAVLQNAFGANGLPLSATVSADGKTYT
MTSPLPAGWSYVDKDGNALPGSPTLNSGTSNSVRMAYTDPGSGQTYTYEFNLSNVPQTGDSFTLSFNKDGIS
DNRNALNLNALQTKPTVGGTGSTGSTYNDAYGGLVERVGTLTAQARASADASQTVLKQAQDSRDSLSGVSLD
EEAANLIQFQQYYSASAQVIQVARSLFDTLIGAFR*
AnnotationID:361GeneID:352
AnnotatorUID: danlee
Modification Date/Time: 2004-05-20 16:04:03
Gene Name:flgK
Confidence Code:2
GeneProduct:flagellar hook-associated protein 1 FlgK
Cell Localization:()
Synonyms:PA1086
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1256
Secondary Functional Category(ies):Cell wall / LPS / capsule
EC Number:
Status:ACTIVE
Pathway:
Flagella assembly 
Homology:






 
Structural Features:
COG1256, FlgK, Flagellar hook-associated protein [Cell motility and secretion]
             CD-Length = 552 residues,  86.2% aligned
             Score =  309 bits (793), Expect = 6e-85






   
Genomic Context:
Cluster of flagellar biosynthetic genes from flgB through flgL 






   
Comment:


















    ReferenceID:707




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17849




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:352
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
587
                           PA1086_tr
                           translation of PA1086
                           683
                           98.97
                           99.70
                           0
                           3401.0
     

     
     
28019
                           gb|AAG04475.1|AE004539_17
                           (AE004539) flagellar hook-associated protein 1 FlgK [Pseudomonas aeruginosa PAO1]
                           683
                           98.97
                           99.70
                           0
                           3401.0
     

     
     
28020
                           gb|AAK15324.1|AF332547_1
                           (AF332547) flagellar hook-associated protein 1 FlgK [Pseudomonas aeruginosa]
                           683
                           88.14
                           93.55
                           1
                           3049.0
     

     
     
28021
                           gb|AAN69959.1|AE016634_8
                           (AE016790) flagellar hook-associated protein FlgK [Pseudomonas putida KT2440]
                           702
                           42.02
                           58.54
                           41
                           1281.0
     

     






      

          
GC ORFID: 43354How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1086Glimmer Score: 

          
Start: 476143Stop: 478194

          
Length: 2052


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1086 translation of PA1086Homolog Bit Score 1314.0

          
Other Homologs:  t_PA1086 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCGACCTACTCTCGATAGGCCTGTCCGGCCTCGGCACCAGCCAGACCTGGCTGACCATCACCGGGCAC 
AACATCACCAACGTCAAGACGCCGGGCTACAGCCGCCAGGATGCCATCCAGCAGACCCAGGTCCCGCAGTTC 
AGCGGTGCCGGCTACATGGGCTCGGGCAGCCAGATCGTCGACGTGCGCCGCCTGGCCAGTGACTTCCTCACC 
GGCCAGTTGCGCAACGCCACCAGCCAGAACAGCGAGTTGAGCGCTTTCCTGGGCCAGATCGACCAGCTCAAC 
TCGCTGCTGGCGGATAACACCACCGGGGTCAGCCCGGCGATGCAGCGTTTCTTTTCCGCCTTGCAGACCGCT 
GCGCAGAACCCGTCCTCGACCGAGGCGCGCGAGGCGGTGCTGGCCCAGGCGCAGGGCCTGAGCAAGACCTTC 
AATACCCTCTACGACCAACTGGACAAGCAGAACAGCCTGATCAACCAGCAGTTGGGTGCCCTGACCTCGCAG 
GTGAACAATCTCAGCCAGAGCGTGGCCGAGTACAACGATGCCATCGCCAAGGCGAAATCCGCCGGGGCCGTG 
CCCAACGATCTGCTGGACGCGCGCGACGAAGCGGTGCGCAAGCTCTCCGAGATGGTCGGCGTGACCGCGGTC 
ACCCAGGACGACAACAGCGTCAGCCTGTTCATCGGCAGCGGCCAGCCGCTGGTGGTGGGCAATACCGTTTCG 
ACGCTGAGCGTGGTGCCGGGGCTGGACGACCCGACCCGCTACCAGGTGCAGCTCACGCTTGGCGACTCCACG 
CAAAACGTGACCCGGTTGGTCTCCGGCGGGCAGATGGGCGGCCTGCTGGCGTATCGCGATACCGTCCTGGAT 
AGCTCCTACAACAAGCTCGGCCAACTTGCGCTGACCTTCGCCGATACCGTCAACAAGCAACTGGGGCAGGGC 
CTGGACCTGGCGGGCAAGGCCGGCGCCAACCTGTTCGGCGACATCAACGATCCGGACATCACGGCGCTGCGG 
GTCCTGGCGAAGAACGGCAACACCGGCAATGTCCACGCCAACCTGAACATCACCGATACCAGCAAGCTGAAT 
TCCAGCGATTTCCGCCTGGACTTCGACGGCACCAATTTCACCGCGCGGCGCCTGGGCGACGACGCAAGCATG 
CAGGTCACGGTCAGCGGTACCGGTCCCTACACCCTCTCGTTCAAGGATGCCAACGGCGTCGACCAGGGCTTC 
AGCGTGACCCTCGACCAACTGCCGGCCGCGGGCGACCGCTTCACCCTGCAGCCGACCCGGCGCGGCGCCAGC 
GATATCGAGACAACCCTGAAGAACGCTTCGCAACTGGCTTTCGCCGGGTCCGCTCGCGCCGAGGCAACCACC 
AACAACCGCGGCAGCGGCGCCATCGGCCAGCCGAACCTGGTCGGCGGACCGTCGCCGATCGATCCGGCGGTT 
CTGCAGAACGCCTTCGGCGCCAATGGTCTGCCGCTGAGCGCGACGGTCAGCGCGGATGGCAAGACCTACACC 
ATGACCTCGCCGCTGCCGGCCGGTTGGAGTTATGTCGACAAGGACGGCAACGCGCTGCCCGGCAGTCCGACG 
CTGAACAGCGGTACCAGCAACAGCGTGCGCATGGCCTATACCGATCCGGGCAGCGGCCAGACCTATACCTAC 
GAGTTCAACCTGTCCAACGTGCCGCAGACTGGCGACAGTTTCACCCTGAGCTTCAACAAGGACGGCATTTCG 
GACAACCGCAACGCCCTCAACCTGAATGCCTTGCAGACCAAGCCGACGGTGGGCGGTACCGGCAGCACGGGG 
TCTACCTACAACGACGCCTACGGTGGACTGGTGGAGCGGGTCGGCACCCTGACCGCCCAGGCGCGCGCCAGC 
GCCGACGCCTCGCAGACCGTGCTCAAGCAGGCGCAGGACAGCCGCGACTCGCTGTCCGGGGTGAGCCTCGAC 
GAAGAGGCGGCCAACCTGATCCAGTTCCAGCAGTACTACAGCGCGTCGGCCCAGGTCATTCAAGTTGCCCGC 
TCGCTGTTCGATACCCTGATAGGCGCGTTCCGCTGA