ViewGene
Locus Name:
GeneID:
Location:

GeneID:3514
TrivialName: PA14_43340
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_43340
PAO1 Ortholog Locus:PA1637
Sequence Length:693
Protein Length:230
Start: 3860205
Stop: 3859513
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05026.1|AE004591_6 (AE004591) two-component response regulator KdpE [Pseudomonas aeruginosa PAO1]
Identities = 229/230 (99%), Positives = 230/230 (100%)
Sequence:
ATGACCCAATTGCAGAACAGCATCCTGCTGATCGACGACGAACCGCAGATCCGCAAGTTCCTCCGTATCAGC
CTCAATGCCCAGGGCTACCGGGTACTCGAGGCCGGCACCGGCGAAGAGGGGCTGGCCCAGGCCGCGCTGAAC
CGGCCGGACCTGGTGGTGCTCGACCTCGGCCTGCCGGACCGCGACGGCCAGGACATCCTCCGCGACCTGCGC
GAATGGTCGCAGGTGCCGGTGCTGGTGCTGTCGGTGCGCGCCAGCGAGGGCGAGAAGGTGCTGGCCCTGGAC
GGTGGCGCCAACGACTACGTGACCAAGCCGTTCGGCATCCAGGAGTTCCTCGCCCGCGTGCGCGTCCTGCTG
CGCCAGGCGGCGCAGGGCGAAAGCCCGGAGGCGGCGGTGGCGGTCGGCCCGTTGCAGGTGGACTTCGCCTAT
CGCCGGGTCACCCTGGAGGGTGCGGAAGTGGCGCTGACGCGTAAGGAGTACGCGGTGCTCGCCGCGCTGGCA
CGCCATCTCGGACGGGTAGTGACCCAGCAGCAACTGCTCAAGGATATCTGGGGCCCGACCCACGTCGAAGAC
ACCCATTACCTGCGGGTCGTGGTCGGCCACCTGCGGCAGAAACTCGGCGACGATCCGGCCAGCCCGCGCTTC
CTGGTGACCGAGGCGGGCGTCGGCTATCGCCTGCGCGACAGCTGA
Translation:
MTQLQNSILLIDDEPQIRKFLRISLNAQGYRVLEAGTGEEGLAQAALNRPDLVVLDLGLPDRDGQDILRDLR
EWSQVPVLVLSVRASEGEKVLALDGGANDYVTKPFGIQEFLARVRVLLRQAAQGESPEAAVAVGPLQVDFAY
RRVTLEGAEVALTRKEYAVLAALARHLGRVVTQQQLLKDIWGPTHVEDTHYLRVVVGHLRQKLGDDPASPRF
LVTEAGVGYRLRDS*
AnnotationID:3522GeneID:3514
AnnotatorUID: diggins
Modification Date/Time: 2005-02-27 15:03:40
Gene Name:kdpE
Confidence Code:2
GeneProduct:two-component response regulator KdpE
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:KDP operon transcriptional regulatory protein KdpE
Functional Category Confidence Code:5
COGs:COG0745,cd00156,cd00383,pfam00486
Secondary Functional Category(ies):transcriptional regulators
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69739.1|AE016610_5 (AE016789) transcriptional regulatory protein KdpE [Pseudomonas
           putida KT2440]
          Length = 231

 Score =  355 bits (911), Expect = 1e-97
 Identities = 176/221 (79%), Positives = 195/221 (88%)

>gb|AAO55762.1| (AE016863) KDP operon transcriptional regulatory protein kdpE
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 230

 Score =  347 bits (889), Expect = 4e-95
 Identities = 170/221 (76%), Positives = 193/221 (87%)

>gb|AAM35649.1| (AE011706) two-component system regulatory protein [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 238

 Score =  287 bits (734), Expect = 4e-77
 Identities = 142/220 (64%), Positives = 174/220 (79%), Gaps = 1/220 (0%)






 
Structural Features:
COG0745, OmpR, Response regulators consisting of a CheY-like receiver domain 
and a winged-helix DNA-binding domain 
[Signal transduction mechanisms / Transcription]
CD-Length = 229 residues,  98.3% aligned
Score =  208 bits (531), Expect = 4e-55
Identities = 102/226 (45%), Positives = 145/226 (64%), Gaps = 5/226 (2%)

cd00156, REC, Signal receiver domain
CD-Length = 113 residues, 100.0% aligned
Score =  109 bits (273), Expect = 4e-25
Identities = 51/113 (45%), Positives = 76/113 (67%), Gaps = 1/113 (0%)

cd00383, trans_reg_C, Effector domain of response regulator.
CD-Length = 95 residues, 100.0% aligned
Score =  103 bits (259), Expect = 2e-23

pfam00486, Trans_reg_C, Transcriptional regulatory protein, C terminal.
CD-Length = 75 residues, 100.0% aligned
Score = 77.5 bits (191), Expect = 1e-15
Identities = 32/77 (41%), Positives = 47/77 (61%), Gaps = 2/77 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:7031




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24173




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37258




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3514_cdd.html


    		




 
URL:

















    ReferenceID:37883




  
Author/Investigator(s):
        Treuner-Lange A, Kuhn A, Durre P.





  
Title:
        The kdp system of Clostridium acetobutylicum: cloning, sequencing, and transcriptional regulation in response to potassium concentration.




  
PubMed:
        9226259


MedLine:
        




    
Source:
J Bacteriol. 1997 Jul;179(14):4501-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9226259

















    ReferenceID:37891




  
Author/Investigator(s):
        Walderhaug MO, Polarek JW, Voelkner P, Daniel JM, Hesse JE, Altendorf K, Epstein W.





  
Title:
        KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators.




  
PubMed:
        1532388


MedLine:
        




    
Source:
J Bacteriol. 1992 Apr;174(7):2152-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1532388

















    ReferenceID:37894




  
Author/Investigator(s):
        Polarek JW, Williams G, Epstein W.





  
Title:
        The products of the kdpDE operon are required for expression of the Kdp ATPase of Escherichia coli.




  
PubMed:
        1532387


MedLine:
        




    
Source:
J Bacteriol. 1992 Apr;174(7):2145-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1532387









           
Homologs By Global Alignment 

           

       



     
Gene ID:3514
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5601
                           PA1637_tr
                           translation of PA1637
                           230
                           99.56
                           99.99
                           0
                           1150.0
     

     
     
156115
                           gb|AAG05026.1|AE004591_6
                           (AE004591) two-component response regulator KdpE [Pseudomonas aeruginosa PAO1]
                           230
                           99.56
                           99.99
                           0
                           1150.0
     

     
     
156116
                           gb|AAN69739.1|AE016610_5
                           (AE016789) transcriptional regulatory protein KdpE [Pseudomonas putida KT2440]
                           232
                           76.72
                           85.34
                           3
                           904.0
     

     
     
156117
                           gb|AAO55762.1|
                           (AE016863) KDP operon transcriptional regulatory protein kdpE [Pseudomonas syringae pv. tomato str. DC3000]
                           231
                           75.32
                           85.71
                           2
                           887.0
     

     
     
156119
                           gb|AAM40022.1|
                           (AE012169) two-component system regulatory protein [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           238
                           60.08
                           74.36
                           8
                           720.0
     

     






      

          
GC ORFID: 46516How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1637Glimmer Score: 

          
Start: 1093704Stop: 1094396

          
Length: 693


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1637 translation of PA1637Homolog Bit Score 447.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1038 (ORF: BLASTX 350.0), t_PA1637 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCCAATTGCAGAACAGCATCCTGCTGATCGACGACGAACCGCAGATCCGCAAGTTCCTCCGTATCAGC 
CTCAATGCCCAGGGCTACCGGGTACTCGAGGCCGGCACCGGCGAAGAGGGGCTGGCCCAGGCCGCGCTGAAC 
CGGCCGGACCTGGTGGTGCTCGACCTCGGCCTGCCGGACCGCGACGGCCAGGACATCCTCCGCGACCTGCGC 
GAATGGTCGCAGGTGCCGGTGCTGGTGCTGTCGGTGCGCGCCAGCGAGGGCGAGAAGGTGCTGGCCCTGGAC 
GGTGGCGCCAACGACTACGTGACCAAGCCGTTCGGCATCCAGGAGTTCCTCGCCCGCGTGCGCGTCCTGCTG 
CGCCAGGCGGCGCAGGGCGAAAGCCCGGAGGCGGCGGTGGCGGTCGGCCCGTTGCAGGTGGACTTCGCCTAT 
CGCCGGGTCACCCTGGAGGGTGCGGAAGTGGCGCTGACGCGTAAGGAGTACGCGGTGCTCGCCGCGCTGGCA 
CGCCATCTCGGACGGGTAGTGACCCAGCAGCAACTGCTCAAGGATATCTGGGGCCCGACCCACGTCGAAGAC 
ACCCATTACCTGCGGGTCGTGGTCGGCCACCTGCGGCAGAAACTCGGCGACGATCCGGCCAGCCCGCGCTTC 
CTGGTGACCGAGGCGGGCGTCGGCTATCGCCTGCGCGACAGCTGA