ViewGene
Locus Name:
GeneID:
Location:

GeneID:3501
TrivialName: PA14_68390
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_68390
PAO1 Ortholog Locus:PA5177
Sequence Length:666
Protein Length:221
Start: 6101809
Stop: 6102474
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08562.1|AE004930_8 (AE004930) probable hydrolase [Pseudomonas aeruginosa PAO1]
Identities = 219/221 (99%), Positives = 220/221 (99%)
Sequence:
ATGCCCACCCTGCCCTGGTCCGCCATCGACACCGTGCTGCTCGACATGGACGGCACCCTGCTCGACCTGCAC
TTCGACAACCACTTCTGGCTGGAACACCTGCCCCAGCGCTATGCCGAACGCAACGGCCTGAGTCGCGCCGAA
GCCGATGCCGTGCTCGAGCCGCTGTTCCGCGAACACGCTGGCCAGCTCAACTGGTACTGCCTGGATTTCTGG
AGCCGGGAATTGGACCTGTCGATCCGCGAACTCAAGCATGAGGTGGCGCACCTGATCTCGCTGCGTCCGCAC
GCCGATACCTTCCTCGCCGCCCTGCGCCAGGCCGGCAAGCGCGTGGCGCTGATCACCAACGCGCACCGCGAC
TCGCTGTCGCTGAAGCTGGAACGGATCGAACTGGCGCCCTATTTCGACCGACTGATCAGCTCCCACGACTAC
GGCTACCCGAAGGAGGACGCGCGCTTCTGGAGCGCCCTGCAAGCCGATTTCGGCTTCGACCCGCAGCGCAGC
CTGTTCATCGACGACAGCCTGCCGATCCTGCGCAGCGCCCGCGCCTATGGTGTCGCCCACCTGTTGGCGGTA
CGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTG
CTCGAAGGACTGCCGTAG
Translation:
MPTLPWSAIDTVLLDMDGTLLDLHFDNHFWLEHLPQRYAERNGLSRAEADAVLEPLFREHAGQLNWYCLDFW
SRELDLSIRELKHEVAHLISLRPHADTFLAALRQAGKRVALITNAHRDSLSLKLERIELAPYFDRLISSHDY
GYPKEDARFWSALQADFGFDPQRSLFIDDSLPILRSARAYGVAHLLAVRHPDSRGQVRDTEEFAAQEDYRRL
LEGLP*
AnnotationID:3509GeneID:3501
AnnotatorUID: diggins
Modification Date/Time: 2005-03-15 16:04:55
Gene Name:
Confidence Code:3
GeneProduct:putative hydrolase
Cell Localization:()
Synonyms:PA5177
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:putative phosphatase
Functional Category Confidence Code:5
COGs:COG1011,pfam00702,COG0546,COG0637
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65890.1|AE016217_2 (AE016775) hydrolase, haloacid dehalogenase-like family
           [Pseudomonas putida KT2440]
          Length = 220

 Score =  353 bits (907), Expect = 3e-97
 Identities = 171/220 (77%), Positives = 190/220 (86%)

>gb|AAO58825.1| (AE016875) HAD-superfamily hydrolase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 220

 Score =  330 bits (847), Expect = 3e-90
 Identities = 157/220 (71%), Positives = 187/220 (85%)








 
Structural Features:
COG1011, COG1011, Predicted hydrolase (HAD superfamily)
CD-Length = 229 residues,  98.7% aligned
Score = 90.4 bits (223), Expect = 2e-19
Identities = 53/227 (23%), Positives = 77/227 (33%), Gaps = 13/227 (5%)

pfam00702, Hydrolase, haloacid dehalogenase-like hydrolase
CD-Length = 197 residues, 100.0% aligned
Score = 78.9 bits (193), Expect = 5e-16
Identities = 41/197 (20%), Positives = 65/197 (32%), Gaps = 12/197 (6%)

COG0637, COG0637, Predicted phosphatase/phosphohexomutase
CD-Length = 221 residues,  88.2% aligned
Score = 68.6 bits (167), Expect = 5e-13
Identities = 48/207 (23%), Positives = 82/207 (39%), Gaps = 24/207 (11%)

COG0546, Gph, Predicted phosphatases
CD-Length = 220 residues,  84.1% aligned
Score = 65.5 bits (159), Expect = 5e-12
Identities = 51/190 (26%), Positives = 77/190 (40%), Gaps = 12/190 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7005




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24147




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34892




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3501_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3501
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5581
                           PA5177_tr
                           translation of PA5177
                           221
                           99.09
                           99.54
                           0
                           1157.0
     

     
     
155749
                           gb|AAG08562.1|AE004930_8
                           (AE004930) probable hydrolase [Pseudomonas aeruginosa PAO1]
                           221
                           99.09
                           99.54
                           0
                           1157.0
     

     
     
155750
                           gb|AAN65890.1|AE016217_2
                           (AE016775) hydrolase, haloacid dehalogenase-like family [Pseudomonas putida KT2440]
                           221
                           77.37
                           85.97
                           1
                           907.0
     

     
     
155751
                           gb|AAO58825.1|
                           (AE016875) HAD-superfamily hydrolase [Pseudomonas syringae pv. tomato str. DC3000]
                           221
                           71.04
                           84.61
                           1
                           847.0
     

     
     
155752
                           gb|AAN53264.1|AE015467_9
                           (AE015467) HAD-superfamily hydrolase, subfamily IA, variant 3 protein family [Shewanella oneidensis MR-1]
                           222
                           48.64
                           60.81
                           3
                           542.0
     

     






      

          
GC ORFID: 46503How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5177Glimmer Score: 

          
Start: 5365495Stop: 5366160

          
Length: 666


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA5177 translation of PA5177Homolog Bit Score 450.0

          
Other Homologs:  psyr_15may02_Scaffold14_revised_gene2290 (ORF: BLASTX 330.0), t_PA5177 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCACCCTGCCCTGGTCCGCCATCGACACCGTGCTGCTCGACATGGACGGCACCCTGCTCGACCTGCAC 
TTCGACAACCACTTCTGGCTGGAACACCTGCCCCAGCGCTATGCCGAACGCAACGGCCTGAGTCGCGCCGAA 
GCCGATGCCGTGCTCGAGCCGCTGTTCCGCGAACACGCTGGCCAGCTCAACTGGTACTGCCTGGATTTCTGG 
AGCCGGGAATTGGACCTGTCGATCCGCGAACTCAAGCATGAGGTGGCGCACCTGATCTCGCTGCGTCCGCAC 
GCCGATACCTTCCTCGCCGCCCTGCGCCAGGCCGGCAAGCGCGTGGCGCTGATCACCAACGCGCACCGCGAC 
TCGCTGTCGCTGAAGCTGGAACGGATCGAACTGGCGCCCTATTTCGACCGACTGATCAGCTCCCACGACTAC 
GGCTACCCGAAGGAGGACGCGCGCTTCTGGAGCGCCCTGCAAGCCGATTTCGGCTTCGACCCGCAGCGCAGC 
CTGTTCATCGACGACAGCCTGCCGATCCTGCGCAGCGCCCGCGCCTATGGTGTCGCCCACCTGTTGGCGGTA 
CGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTG 
CTCGAAGGACTGCCGTAG