ViewGene
Locus Name:
GeneID:
Location:

GeneID:3483
TrivialName: PA14_26890
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_26890
PAO1 Ortholog Locus:PA2876
Sequence Length:699
Protein Length:232
Start: 2339177
Stop: 2339875
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06264.1|AE004714_1 (AE004714) orotidine 5'-phosphate decarboxylase [Pseudomonas aeruginosa PAO1]
Identities = 232/232 (100%), Positives = 232/232 (100%)
Sequence:
ATGTCCGCCTGCCAGTCCCCCATCATCGTCGCCCTGGATTTCCCCACCCGTGAGGCCGCCCTGGCGCTGGCC
GATCAGCTGGACCCGAAACTGTGCCGGGTGAAGGTCGGCAAGGAACTCTTCACCAGTTGTGCCGCGGGTATA
GTCGAGACCTTGCGCGGCAAGGGCTTCGAGGTGTTCCTCGACCTGAAATTCCATGACATCCCGAACACCACG
GCGATGGCGGTGAAGGCCGCCGCCGAGATGGGCGTGTGGATGGTCAACGTGCACTGCTCCGGCGGCCTGCGC
ATGATGGCCGCCTGCCGCGAGACGCTGGAGGCCTTCAGCGGACCCAGGCCGTTGCTCATCGGCGTGACCGTG
CTGACCAGCATGGAGCGCGAGGACCTGGCGGGCATCGGCCTCGACATCGAGCCCCAGGAGCAGGTACTGCGC
CTCGCCGCCCTGGCGCAGAAGGCCGGCATGGACGGCCTGGTCTGCTCGGCCCAGGAAGCGCCGGCGCTGAAG
GCTGCCCATCCGGGCCTGCAACTGGTCACGCCGGGCATCCGCCCGGCCGGCAGCGCCCAGGACGACCAGCGG
CGTATCCTCACCCCGCGCCAGGCCCTGGACGCCGGCTCCGACTACCTGGTGATCGGCCGGCCGATCAGCCAG
GCCGCCGACCCGGCCAAGGCGCTGGCGGCGATCGTCGCCGAACTGGGCTGA
Translation:
MSACQSPIIVALDFPTREAALALADQLDPKLCRVKVGKELFTSCAAGIVETLRGKGFEVFLDLKFHDIPNTT
AMAVKAAAEMGVWMVNVHCSGGLRMMAACRETLEAFSGPRPLLIGVTVLTSMEREDLAGIGLDIEPQEQVLR
LAALAQKAGMDGLVCSAQEAPALKAAHPGLQLVTPGIRPAGSAQDDQRRILTPRQALDAGSDYLVIGRPISQ
AADPAKALAAIVAELG*
AnnotationID:3491GeneID:3483
AnnotatorUID: diggins
Modification Date/Time: 2005-04-07 07:07:28
Gene Name:pyrF
Confidence Code:1
GeneProduct:orotidine-5'-phosphate decarboxylase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0284,pfam00215
Secondary Functional Category(ies):
EC Number:4.1.1.23
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67434.1|AE016370_9 (AE016780) orotidine 5-phosphate decarboxylase [Pseudomonas putida
           KT2440]
          Length = 233

 Score =  412 bits (1059), Expect = e-115
 Identities = 210/231 (90%), Positives = 220/231 (95%)

>gb|AAO55546.1| (AE016863) orotidine 5-phosphate decarboxylase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 212

 Score =  379 bits (974), Expect = e-105
 Identities = 193/211 (91%), Positives = 201/211 (95%)

>dbj|BAC60289.1| (AP005080) orotidine 5'-phosphate decarboxylase [Vibrio
           parahaemolyticus]
          Length = 233

 Score =  278 bits (710), Expect = 2e-74
 Identities = 142/221 (64%), Positives = 169/221 (76%), Gaps = 1/221 (0%)








 
Structural Features:
COG0284, PyrF, Orotidine-5'-phosphate decarboxylase 
[Nucleotide transport and metabolism]
CD-Length = 240 residues,  95.4% aligned
Score =  238 bits (609), Expect = 3e-64
Identities = 115/233 (49%), Positives = 150/233 (64%), Gaps = 5/233 (2%)

pfam00215, OMPdecase, Orotidine 5'-phosphate decarboxylase / HUMPS family.
CD-Length = 215 residues, 100.0% aligned
Score =  195 bits (496), Expect = 6e-51
Identities = 91/222 (40%), Positives = 118/222 (53%), Gaps = 7/222 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6969




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24111




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36196




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3483_cdd.html


    		




 
URL:

















    ReferenceID:36410




  
Author/Investigator(s):
        Strych U, Wohlfarth S, Winkler UK.





  
Title:
        Orotidine-5'-monophosphate decarboxylase from Pseudomonas aeruginosa PAO1: cloning, overexpression, and enzyme characterization.




  
PubMed:
        7765522


MedLine:
        




    
Source:
Curr Microbiol. 1994 Dec;29(6):353-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7765522









           
Homologs By Global Alignment 

           

       



     
Gene ID:3483
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5550
                           PA2876_tr
                           translation of PA2876
                           232
                           99.99
                           99.99
                           0
                           1166.0
     

     
     
154496
                           gb|AAG06264.1|AE004714_1
                           (AE004714) orotidine 5'-phosphate decarboxylase [Pseudomonas aeruginosa PAO1]
                           232
                           99.99
                           99.99
                           0
                           1166.0
     

     
     
154497
                           emb|CAA46564.1|
                           (X65613) orotidine-5'-phosphate decarboxylase [Pseudomonas aeruginosa]
                           232
                           99.56
                           99.56
                           0
                           1158.0
     

     
     
154498
                           gb|AAN67434.1|AE016370_9
                           (AE016780) orotidine 5-phosphate decarboxylase [Pseudomonas putida KT2440]
                           233
                           90.12
                           94.42
                           1
                           1057.0
     

     
     
154499
                           gb|AAO55546.1|
                           (AE016863) orotidine 5-phosphate decarboxylase [Pseudomonas syringae pv. tomato str. DC3000]
                           232
                           83.18
                           86.63
                           20
                           974.0
     

     






      

          
GC ORFID: 46485How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2876Glimmer Score: 

          
Start: 2614749Stop: 2614051

          
Length: 699


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2876 translation of PA2876Homolog Bit Score 453.0

          
Other Homologs:  t_PA2876 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCGCCTGCCAGTCCCCCATCATCGTCGCCCTGGATTTCCCCACCCGTGAGGCCGCCCTGGCGCTGGCC 
GATCAGCTGGACCCGAAACTGTGCCGGGTGAAGGTCGGCAAGGAACTCTTCACCAGTTGTGCCGCGGGTATA 
GTCGAGACCTTGCGCGGCAAGGGCTTCGAGGTGTTCCTCGACCTGAAATTCCATGACATCCCGAACACCACG 
GCGATGGCGGTGAAGGCCGCCGCCGAGATGGGCGTGTGGATGGTCAACGTGCACTGCTCCGGCGGCCTGCGC 
ATGATGGCCGCCTGCCGCGAGACGCTGGAGGCCTTCAGCGGACCCAGGCCGTTGCTCATCGGCGTGACCGTG 
CTGACCAGCATGGAGCGCGAGGACCTGGCGGGCATCGGCCTCGACATCGAGCCCCAGGAGCAGGTACTGCGC 
CTCGCCGCCCTGGCGCAGAAGGCCGGCATGGACGGCCTGGTCTGCTCGGCCCAGGAAGCGCCGGCGCTGAAG 
GCTGCCCATCCGGGCCTGCAACTGGTCACGCCGGGCATCCGCCCGGCCGGCAGCGCCCAGGACGACCAGCGG 
CGTATCCTCACCCCGCGCCAGGCCCTGGACGCCGGCTCCGACTACCTGGTGATCGGCCGGCCGATCAGCCAG 
GCCGCCGACCCGGCCAAGGCGCTGGCGGCGATCGTCGCCGAACTGGGCTGA