ViewGene
Locus Name:
GeneID:
Location:

GeneID:3473
TrivialName: PA14_70750
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_70750
PAO1 Ortholog Locus:PA5360
Sequence Length:690
Protein Length:229
Start: 6299698
Stop: 6300387
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08745.1|AE004948_1 (AE004948) two-component response regulator PhoB [Pseudomonas aeruginosa PAO1]
Identities = 229/229 (100%), Positives = 229/229 (100%)
Sequence:
ATGGTTGGCAAGACAATCCTCATCGTTGATGACGAAGCACCGATTCGCGAGATGATCGCCGTGGCCCTGGAG
ATGGCCGGCTACGAGTGCCTGGAGGCGGAAAATACCCAGCAGGCGCACGCGGTGATCGTCGACCGCAAGCCG
GACCTGATCCTGCTCGACTGGATGCTCCCCGGCACTTCCGGCATCGAACTGGCGCGACGCCTGAAGCGTGAC
GAACTGACCGTCGACATCCCGATCATCATGCTCACCGCCAAGGGCGAGGAGGACAACAAGATCCAGGGCCTG
GAGGTCGGTGCCGACGACTACATCACCAAGCCGTTCTCGCCCCGCGAGCTGGTCGCCCGCCTGAAGGCCGTG
CTGCGCCGCACCGGGCCTGGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTGGACCCGATCAGC
CACCGCGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCCACCGAGTACCGCCTGCTGCAGTTCTTCATG
ACTCACCAGGAACGCGCCTATACCCGCGGCCAACTGCTCGACCAGGTCTGGGGCGGCAACGTCTATGTCGAG
GAGCGCACCGTCGACGTGCATATCCGCCGCCTGCGCAAGGCGCTCGGCGAGGTCTACGAAAATCTGGTTCAG
ACTGTCCGCGGGACCGGCTATCGTTTCTCCACCAAGAGCTGA
Translation:
MVGKTILIVDDEAPIREMIAVALEMAGYECLEAENTQQAHAVIVDRKPDLILLDWMLPGTSGIELARRLKRD
ELTVDIPIIMLTAKGEEDNKIQGLEVGADDYITKPFSPRELVARLKAVLRRTGPGDSEAPIEVGGLLLDPIS
HRVTIDGKPAEMGPTEYRLLQFFMTHQERAYTRGQLLDQVWGGNVYVEERTVDVHIRRLRKALGEVYENLVQ
TVRGTGYRFSTKS*
AnnotationID:3481GeneID:3473
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 19:07:39
Gene Name:phoB
Confidence Code:1
GeneProduct:two-component response regulator PhoB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:Phosphate regulon transcriptional regulatory protein phoB
Functional Category Confidence Code:5
COGs:COG0745,cd00156,pfam00486
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70885.1|AE016732_10 (AE016794) DNA-binding response regulator PhoB [Pseudomonas putida
           KT2440]
          Length = 242

 Score =  442 bits (1136), Expect = e-124
 Identities = 221/229 (96%), Positives = 225/229 (98%)

>gb|AAO58896.1| (AE016875) phosphate regulon transcriptional regulatory protein
           PhoB [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 229

 Score =  433 bits (1114), Expect = e-121
 Identities = 215/229 (93%), Positives = 223/229 (97%)

>gb|AAF93884.1| (AE004158) DNA-binding response regulator PhoB [Vibrio cholerae]
          Length = 229

 Score =  300 bits (767), Expect = 6e-81
 Identities = 144/229 (62%), Positives = 186/229 (81%), Gaps = 1/229 (0%)








 
Structural Features:
COG0745, OmpR, Response regulators consisting of a CheY-like receiver domain and 
a winged-helix DNA-binding domain 
[Signal transduction mechanisms / Transcription]
CD-Length = 229 residues,  98.7% aligned
Score =  231 bits (589), Expect = 7e-62
Identities = 100/228 (43%), Positives = 140/228 (61%), Gaps = 7/228 (3%)

cd00156, REC, Signal receiver domain
CD-Length = 113 residues, 100.0% aligned
Score =  125 bits (315), Expect = 4e-30
Identities = 61/115 (53%), Positives = 88/115 (76%), Gaps = 2/115 (1%)

pfam00486, Trans_reg_C, Transcriptional regulatory protein, C terminal.
CD-Length = 75 residues, 100.0% aligned
Score = 92.5 bits (230), Expect = 4e-20
Identities = 34/75 (45%), Positives = 50/75 (66%)







   
Genomic Context:






   
Comment:


















    ReferenceID:6949




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24091




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34754




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3473_cdd.html


    		




 
URL:

















    ReferenceID:35674




  
Author/Investigator(s):
        Anba J, Bidaud M, Vasil ML, Lazdunski A.





  
Title:
        Nucleotide sequence of the Pseudomonas aeruginosa phoB gene, the regulatory gene for the phosphate regulon.




  
PubMed:
        2115874


MedLine:
        




    
Source:
J Bacteriol. 1990 Aug;172(8):4685-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2115874

















    ReferenceID:35677




  
Author/Investigator(s):
        Watson GM, Scanlan DJ, Mann NH.





  
Title:
        Characterization of the genes encoding a phosphate-regulated two component sensory system in the marine cyanobacterium Synechococcus sp. WH7803.




  
PubMed:
        8759795


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1996 Aug 15;142(1):105-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8759795









           
Homologs By Global Alignment 

           

       



     
Gene ID:3473
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5534
                           PA5360_tr
                           translation of PA5360
                           229
                           99.99
                           99.99
                           0
                           1169.0
     

     
     
154104
                           gb|AAG08745.1|AE004948_1
                           (AE004948) two-component response regulator PhoB [Pseudomonas aeruginosa PAO1]
                           229
                           99.99
                           99.99
                           0
                           1169.0
     

     
     
154106
                           gb|AAO58896.1|
                           (AE016875) phosphate regulon transcriptional regulatory protein PhoB [Pseudomonas syringae pv. tomato str. DC3000]
                           229
                           93.88
                           97.37
                           0
                           1114.0
     

     
     
154105
                           gb|AAN70885.1|AE016732_10
                           (AE016794) DNA-binding response regulator PhoB [Pseudomonas putida KT2440]
                           242
                           91.32
                           92.97
                           13
                           1136.0
     

     
     
154107
                           gb|AAM75361.1|AF521648_1
                           (AF521648) PhoB [Pseudomonas syringae]
                           230
                           90.86
                           95.65
                           1
                           1083.0
     

     






      

          
GC ORFID: 46475How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5360Glimmer Score: 

          
Start: 5563387Stop: 5564076

          
Length: 690


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5360 translation of PA5360Homolog Bit Score 454.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene2897 (ORF: BLASTX 434.0), t_PA4032 (ORF: BLASTX 114.0), t_PA5360 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGTTGGCAAGACAATCCTCATCGTTGATGACGAAGCACCGATTCGCGAGATGATCGCCGTGGCCCTGGAG 
ATGGCCGGCTACGAGTGCCTGGAGGCGGAAAATACCCAGCAGGCGCACGCGGTGATCGTCGACCGCAAGCCG 
GACCTGATCCTGCTCGACTGGATGCTCCCCGGCACTTCCGGCATCGAACTGGCGCGACGCCTGAAGCGTGAC 
GAACTGACCGTCGACATCCCGATCATCATGCTCACCGCCAAGGGCGAGGAGGACAACAAGATCCAGGGCCTG 
GAGGTCGGTGCCGACGACTACATCACCAAGCCGTTCTCGCCCCGCGAGCTGGTCGCCCGCCTGAAGGCCGTG 
CTGCGCCGCACCGGGCCTGGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTGGACCCGATCAGC 
CACCGCGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCCACCGAGTACCGCCTGCTGCAGTTCTTCATG 
ACTCACCAGGAACGCGCCTATACCCGCGGCCAACTGCTCGACCAGGTCTGGGGCGGCAACGTCTATGTCGAG 
GAGCGCACCGTCGACGTGCATATCCGCCGCCTGCGCAAGGCGCTCGGCGAGGTCTACGAAAATCTGGTTCAG 
ACTGTCCGCGGGACCGGCTATCGTTTCTCCACCAAGAGCTGA