ViewGene
Locus Name:
GeneID:
Location:

GeneID:3455
TrivialName: PA14_05000
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05000
PAO1 Ortholog Locus:PA0382
Sequence Length:672
Protein Length:223
Start: 439406
Stop: 440077
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03771.1|AE004476_1 (AE004476) DNA mismatch repair protein MicA [Pseudomonas aeruginosa PAO1]
Identities = 221/223 (99%), Positives = 222/223 (99%)
Sequence:
ATGCGCACCGTGAAGAGCTTCGTGATGCGCGCCGGGCGCATGACCGAAGGCCAGCAGCGTGGCCTCGACCTG
GGCTGGCCGAAGTTCGGCCTGGAACTGAGCGATGAGGTTCAGGACTTCGACGCGATCTTCGGTCGCCAGGCG
CCGCGGACTTTCGAGATCGGTTTCGGCATGGGCCATTCCACCCTGGAAATGGCCGCCGCCGCGCCTGACATC
GACTTCATCGGCGTCGAGGTGCACAAGCCGGGCGTCGGCGCGCTGCTCAACGGCCTGCTGACCCAGGGCCTG
GGCAACGTGCGGGTGTATAGCTGCGACGCGCTGGAAGTGCTGCGCCACTGCGTGGCCGACGCCAGCCTCGAT
CGGCTGCTGCTGTTCTTCCCCGATCCCTGGCACAAGAAGCGCCACCACAAGCGGCGGATCGTCCAGCCGGAA
TTCGCCGAACTGGTGCGGCGCAAGCTGAAGGTCGGCGGCGTGCTGCACATGGCCACCGACTGGGAGCCCTAT
GCCGAGCACATGCTGGACGTCATGAGCGCGGCGCCGGGCTACCGCAACCAGGCCGAAGACGGCCGCTTCGTA
GCGCGCCCGCAAGAGCGTCCGGTGACCAAGTTCGAACGGCGCGGGGAGCGCCTGGGACATGGCGTCTGGGAC
CTCAAGTTCGAGCGCATCGACTGA
Translation:
MRTVKSFVMRAGRMTEGQQRGLDLGWPKFGLELSDEVQDFDAIFGRQAPRTFEIGFGMGHSTLEMAAAAPDI
DFIGVEVHKPGVGALLNGLLTQGLGNVRVYSCDALEVLRHCVADASLDRLLLFFPDPWHKKRHHKRRIVQPE
FAELVRRKLKVGGVLHMATDWEPYAEHMLDVMSAAPGYRNQAEDGRFVARPQERPVTKFERRGERLGHGVWD
LKFERID*
AnnotationID:3463GeneID:3455
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 06:06:31
Gene Name:micA
Confidence Code:2
GeneProduct:DNA mismatch repair protein MicA
Cell Localization:()
Synonyms:yggH
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:putative methyltransferase
Functional Category Confidence Code:5
COGs:COG0220,pfam02390
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53979.1| (AE016857) methyltransferase, putative [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 244

 Score =  374 bits (960), Expect = e-103
 Identities = 176/222 (79%), Positives = 194/222 (87%)

>gb|AAN81995.1|AE016766_83 (AE016766) Hypothetical methyltransferase yggH [Escherichia coli
           CFT073]
          Length = 239

 Score =  282 bits (722), Expect = 9e-76
 Identities = 132/222 (59%), Positives = 167/222 (75%)






 
Structural Features:
COG0220, Predicted S-adenosylmethionine-dependent methyltransferase 
CD-Length = 227 residues,  98.7% aligned
Score =  253 bits (647), Expect = 1e-68
Identities = 102/224 (45%), Positives = 145/224 (64%), Gaps = 3/224 (1%)

pfam02390, Methyltransf_4, Putative methyltransferase. 
CD-Length = 198 residues,  97.5% aligned
Score =  189 bits (481), Expect = 2e-49
Identities = 76/193 (39%), Positives = 112/193 (58%), Gaps = 4/193 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6913




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24055




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33717




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3455_cdd.html


    		




 
URL:

















    ReferenceID:35388




  
Author/Investigator(s):
        Tsai-Wu JJ, Radicella JP, Lu AL.





  
Title:
        Nucleotide sequence of the Escherichia coli micA gene required for A/G-specific mismatch repair: identity of micA and mutY.




  
PubMed:
        2001994


MedLine:
        




    
Source:
J Bacteriol. 1991 Mar;173(6):1902-10.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2001994









           
Homologs By Global Alignment 

           

       



     
Gene ID:3455
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5513
                           PA0382_tr
                           translation of PA0382
                           223
                           99.10
                           99.55
                           0
                           1179.0
     

     
     
153748
                           gb|AAG03771.1|AE004476_1
                           (AE004476) DNA mismatch repair protein MicA [Pseudomonas aeruginosa PAO1]
                           223
                           99.10
                           99.55
                           0
                           1179.0
     

     
     
153750
                           gb|AAN70668.1|AE016710_13
                           (AE016793) conserved hypothetical protein TIGR00091 [Pseudomonas putida KT2440]
                           240
                           73.75
                           81.66
                           17
                           956.0
     

     
     
153749
                           gb|AAO53979.1|
                           (AE016857) methyltransferase, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           244
                           72.13
                           79.50
                           21
                           958.0
     

     
     
153766
                           gb|AAA72956.1|
                           (M59471) [micA] gene products [Escherichia coli]
                           230
                           57.82
                           71.73
                           7
                           690.0
     

     






      

          
GC ORFID: 46457How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0382Glimmer Score: 

          
Start: 6240748Stop: 6241419

          
Length: 672


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0382 translation of PA0382Homolog Bit Score 458.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6619 (ORF: BLASTX 387.0), t_PA0382 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCACCGTGAAGAGCTTCGTGATGCGCGCCGGGCGCATGACCGAAGGCCAGCAGCGTGGCCTCGACCTG 
GGCTGGCCGAAGTTCGGCCTGGAACTGAGCGATGAGGTTCAGGACTTCGACGCGATCTTCGGTCGCCAGGCG 
CCGCGGACTTTCGAGATCGGTTTCGGCATGGGCCATTCCACCCTGGAAATGGCCGCCGCCGCGCCTGACATC 
GACTTCATCGGCGTCGAGGTGCACAAGCCGGGCGTCGGCGCGCTGCTCAACGGCCTGCTGACCCAGGGCCTG 
GGCAACGTGCGGGTGTATAGCTGCGACGCGCTGGAAGTGCTGCGCCACTGCGTGGCCGACGCCAGCCTCGAT 
CGGCTGCTGCTGTTCTTCCCCGATCCCTGGCACAAGAAGCGCCACCACAAGCGGCGGATCGTCCAGCCGGAA 
TTCGCCGAACTGGTGCGGCGCAAGCTGAAGGTCGGCGGCGTGCTGCACATGGCCACCGACTGGGAGCCCTAT 
GCCGAGCACATGCTGGACGTCATGAGCGCGGCGCCGGGCTACCGCAACCAGGCCGAAGACGGCCGCTTCGTA 
GCGCGCCCGCAAGAGCGTCCGGTGACCAAGTTCGAACGGCGCGGGGAGCGCCTGGGACATGGCGTCTGGGAC 
CTCAAGTTCGAGCGCATCGACTGA