ViewGene
Locus Name:
GeneID:
Location:

GeneID:345
TrivialName: PA14_38690
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_38690
PAO1 Ortholog Locus:PA1997
Sequence Length:1956
Protein Length:651
Start: 3449781
Stop: 3451736
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05385.1|AE004626_4 (AE004626) probable AMP-binding enzyme [Pseudomonas aeruginosa PAO1]
Identities = 645/651 (99%), Positives = 648/651 (99%)
Sequence:
ATGCCCGCACCGCTCTGGAGCCCTTCCGCCGAACGCATCGCCGCCAGTCGCATGGAGGCCTTCCGGCGCTTC
GTCAACCAGCGCCATGCCCTCGGTCTGGCCGACTACCCGGCGCTGCATGCCTGGAGCGTGCAGCGGCGCGAG
GCGTTCTGGCAGGCCATCGTCGACTTCTTCGAGGTGCGCTTCCAGCAACCGCCGCGTGCGGTCCTCGAGGAA
GGCACGCAGATGCCCTCGGCGCGCTGGTTCCCCGGCGCCACGCTGAATTTCGCCGAACACCTGCTGCGCCGC
CGCGATGATGCCCCGGCGCTGATCGCGGTGAGCGAAGACGGTCGCCGCGAGGTGCTCAGCCACGCCGAACTG
GCCCGTCACGTGGCGGGCCTGCAGAAGCGCCTCGCCGCCCTGGGCATAGGCCCCGGCGACCGGGTCGCCGCG
CTGATGCCGAACACCTGGCAGACCGTGGTCGGCATGCTCGCCACCGCCAGCCTCGGCGCCACCTGGTCGAGC
TGCTCGCCGGACTTCGGCACCCAGGGCGTGATCGACCGCTTCGGCCAGATCGAACCGCGCGTGCTGATCGCC
TGCGCCGGCTACCGCTACGCCGGCAAGTCGCTGGACCTGACCGCCAAGCTCAACGAGGTGCTCGCCGGCCTG
CCCGGCCTGGAGCAACTGCTGGTGGTGCCCTACGACCGTCCGCAGGCGCAGCCGCAGGACTACCGCTGCCAG
GCGCGTGTCGCGCTGTGGGACGGGTTCTACCAGGCCGAGGGCGAGCCGGTCTTCACCCCGGTGCCCTTCGAG
CAGCCGCTGTACATCCTCTATTCGTCGGGCACCACCGGGGTACCCAAGTGCATCGTCCATGCCACCGGCGGC
GTGCTCCTGCAACATCTCAAGGAGCACGGCCTGCACACCGACCTGGGCGACGGCGACCGGCTGTTCTACTAC
ACCACCTGCGGCTGGATGATGTGGAACTGGCTGGCCTCCGGCCTGGCGGTGGGCGCCAGCCTGGTGCTCTAC
GACGGCTCGCCGTTCCACCCCGGCCCCGAGCGCCTGCTGGACCTCGTCGACGCCGAGGACATCGCCGTGTTC
GGCGCCAGCGCCAAGTACCTGGCGGCGCTCGAGAAGGCCGGCGTGCGCCCGCGCCACAGCCACCGCCTGGAC
AGCCTGAGGACACTGCTCTCCACCGGTTCGCCGCTGGCCCACGAAAGCTTCGACTACGTCTATCGCGAGCTG
AAGAGCGACCTCTGCCTGTCGTCGATCTCCGGCGGCACCGACATCGTTTCCTGCTTCGCCATCGGCAACCCG
GTGCTGCCGGTGTGGCGCGGCGAACTGCAATGCAAGGCGCTGGGCATGGCCGTGGAAATCTGGGACGACGAC
GGCCGCCGCCTCGCCAGCGGCAAGGGCGAGCTGGTCTGCACCCGGCATTTCCCGTCGATCCCGCTGGGCTTC
TGGAACGACCCGGATGGCACCAGGTTCCACGACGCCTACTTCGCCAGTTTTCCCGGCGTCTGGGCCCATGGC
GACTACGCCGAGGAAACCGCCCACGGCGGGCTGGTCATCCACGGCCGCTCCGACGCGGTGCTCAATCCCGGC
GGCGTGCGCATCGGCACCGCGGAAATCTACCGCCAGGTGGAAAAGGTCGAGGAAGTCCTCGAGTCCATCGCC
ATCGGCCAGGACTGGCAGGGCGATGTGCGTGTGCTGCTGTTCGTCCGCCTGCGCGACGGCGTGCGACTCGAC
GAGCCGCTGCGTGAACGCATCCGCCAGACCATCCGCGCCAACGCCACGCCGCGCCACGTACCGGCGAAGATC
ATCCAGGTCGCCGATATCCCGCGCACCCTGAGCGGCAAGATCGTCGAGCTTGCGGTGCGCAACGTGATCCAC
GGCCGCCCGGTGAAGAACACCGACGCCCTGGCCAACCCGCAGGCGCTGGAGCTTTATCGCGACCTCGCCGAA
CTGCGCGACTGA
Translation:
MPAPLWSPSAERIAASRMEAFRRFVNQRHALGLADYPALHAWSVQRREAFWQAIVDFFEVRFQQPPRAVLEE
GTQMPSARWFPGATLNFAEHLLRRRDDAPALIAVSEDGRREVLSHAELARHVAGLQKRLAALGIGPGDRVAA
LMPNTWQTVVGMLATASLGATWSSCSPDFGTQGVIDRFGQIEPRVLIACAGYRYAGKSLDLTAKLNEVLAGL
PGLEQLLVVPYDRPQAQPQDYRCQARVALWDGFYQAEGEPVFTPVPFEQPLYILYSSGTTGVPKCIVHATGG
VLLQHLKEHGLHTDLGDGDRLFYYTTCGWMMWNWLASGLAVGASLVLYDGSPFHPGPERLLDLVDAEDIAVF
GASAKYLAALEKAGVRPRHSHRLDSLRTLLSTGSPLAHESFDYVYRELKSDLCLSSISGGTDIVSCFAIGNP
VLPVWRGELQCKALGMAVEIWDDDGRRLASGKGELVCTRHFPSIPLGFWNDPDGTRFHDAYFASFPGVWAHG
DYAEETAHGGLVIHGRSDAVLNPGGVRIGTAEIYRQVEKVEEVLESIAIGQDWQGDVRVLLFVRLRDGVRLD
EPLRERIRQTIRANATPRHVPAKIIQVADIPRTLSGKIVELAVRNVIHGRPVKNTDALANPQALELYRDLAE
LRD*
AnnotationID:354GeneID:345
AnnotatorUID: diggins
Modification Date/Time: 2005-03-02 18:06:02
Gene Name:
Confidence Code:3
GeneProduct:putative AMP-(fatty) acid ligase
Cell Localization:()
Synonyms:acsA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:putative acetoacetyl-CoA synthetase
Functional Category Confidence Code:5
COGs:COG0365
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68679.1|AE016497_4 (AE016785) acetoacetyl-CoA synthetase, putative [Pseudomonas putida
           KT2440]
          Length = 650

 Score =  959 bits (2480), Expect = 0.0
 Identities = 456/646 (70%), Positives = 538/646 (83%), Gaps = 1/646 (0%)

>gb|AAM00515.1|AF447493_1 (AF447493) acetoacetyl-CoA synthetase [Azospirillum brasilense]
          Length = 659

 Score =  738 bits (1905), Expect = 0.0
 Identities = 367/648 (56%), Positives = 460/648 (70%), Gaps = 6/648 (0%)

>gb|AAN48372.1|AE011300_5 (AE011300) acetyl-CoA synthetase [Leptospira interrogans serovar
           lai str. 56601]
          Length = 660

 Score =  646 bits (1666), Expect = 0.0
 Identities = 317/662 (47%), Positives = 444/662 (67%), Gaps = 14/662 (2%)






 
Structural Features:
COG0365, Acs, Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases 
[Lipid metabolism]
CD-Length = 528 residues,  98.9% aligned
Score =  452 bits (1164), Expect = 6e-128
Identities = 203/554 (36%), Positives = 277/554 (50%), Gaps = 40/554 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:693




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17835




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32729




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:345_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:345
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
576
                           PA1997_tr
                           translation of PA1997
                           651
                           99.07
                           99.53
                           0
                           3414.0
     

     
     
27776
                           gb|AAG05385.1|AE004626_4
                           (AE004626) probable AMP-binding enzyme [Pseudomonas aeruginosa PAO1]
                           651
                           99.07
                           99.53
                           0
                           3414.0
     

     
     
27777
                           gb|AAN68679.1|AE016497_4
                           (AE016785) acetoacetyl-CoA synthetase, putative [Pseudomonas putida KT2440]
                           651
                           70.19
                           82.79
                           1
                           2481.0
     

     
     
27780
                           emb|CAC45291.1|
                           (AL591784) ACETOACETYL-COA SYNTHETASE PROTEIN [Sinorhizobium meliloti]
                           658
                           57.75
                           69.45
                           15
                           1875.0
     

     
     
27782
                           gb|AAC64548.1|
                           (AF080217) acetoacetyl-CoA synthetase; acetoacetyl-CoA ligase; acyl-activating enzyme [Sinorhizobium meliloti]
                           658
                           57.29
                           69.30
                           15
                           1861.0
     

     






      

          
GC ORFID: 43347How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1997Glimmer Score: 

          
Start: 1504130Stop: 1502175

          
Length: 1956


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1997 translation of PA1997Homolog Bit Score 1319.0

          
Other Homologs:  t_PA1997 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCGCACCGCTCTGGAGCCCTTCCGCCGAACGCATCGCCGCCAGTCGCATGGAGGCCTTCCGGCGCTTC 
GTCAACCAGCGCCATGCCCTCGGTCTGGCCGACTACCCGGCGCTGCATGCCTGGAGCGTGCAGCGGCGCGAG 
GCGTTCTGGCAGGCCATCGTCGACTTCTTCGAGGTGCGCTTCCAGCAACCGCCGCGTGCGGTCCTCGAGGAA 
GGCACGCAGATGCCCTCGGCGCGCTGGTTCCCCGGCGCCACGCTGAATTTCGCCGAACACCTGCTGCGCCGC 
CGCGATGATGCCCCGGCGCTGATCGCGGTGAGCGAAGACGGTCGCCGCGAGGTGCTCAGCCACGCCGAACTG 
GCCCGTCACGTGGCGGGCCTGCAGAAGCGCCTCGCCGCCCTGGGCATAGGCCCCGGCGACCGGGTCGCCGCG 
CTGATGCCGAACACCTGGCAGACCGTGGTCGGCATGCTCGCCACCGCCAGCCTCGGCGCCACCTGGTCGAGC 
TGCTCGCCGGACTTCGGCACCCAGGGCGTGATCGACCGCTTCGGCCAGATCGAACCGCGCGTGCTGATCGCC 
TGCGCCGGCTACCGCTACGCCGGCAAGTCGCTGGACCTGACCGCCAAGCTCAACGAGGTGCTCGCCGGCCTG 
CCCGGCCTGGAGCAACTGCTGGTGGTGCCCTACGACCGTCCGCAGGCGCAGCCGCAGGACTACCGCTGCCAG 
GCGCGTGTCGCGCTGTGGGACGGGTTCTACCAGGCCGAGGGCGAGCCGGTCTTCACCCCGGTGCCCTTCGAG 
CAGCCGCTGTACATCCTCTATTCGTCGGGCACCACCGGGGTACCCAAGTGCATCGTCCATGCCACCGGCGGC 
GTGCTCCTGCAACATCTCAAGGAGCACGGCCTGCACACCGACCTGGGCGACGGCGACCGGCTGTTCTACTAC 
ACCACCTGCGGCTGGATGATGTGGAACTGGCTGGCCTCCGGCCTGGCGGTGGGCGCCAGCCTGGTGCTCTAC 
GACGGCTCGCCGTTCCACCCCGGCCCCGAGCGCCTGCTGGACCTCGTCGACGCCGAGGACATCGCCGTGTTC 
GGCGCCAGCGCCAAGTACCTGGCGGCGCTCGAGAAGGCCGGCGTGCGCCCGCGCCACAGCCACCGCCTGGAC 
AGCCTGAGGACACTGCTCTCCACCGGTTCGCCGCTGGCCCACGAAAGCTTCGACTACGTCTATCGCGAGCTG 
AAGAGCGACCTCTGCCTGTCGTCGATCTCCGGCGGCACCGACATCGTTTCCTGCTTCGCCATCGGCAACCCG 
GTGCTGCCGGTGTGGCGCGGCGAACTGCAATGCAAGGCGCTGGGCATGGCCGTGGAAATCTGGGACGACGAC 
GGCCGCCGCCTCGCCAGCGGCAAGGGCGAGCTGGTCTGCACCCGGCATTTCCCGTCGATCCCGCTGGGCTTC 
TGGAACGACCCGGATGGCACCAGGTTCCACGACGCCTACTTCGCCAGTTTTCCCGGCGTCTGGGCCCATGGC 
GACTACGCCGAGGAAACCGCCCACGGCGGGCTGGTCATCCACGGCCGCTCCGACGCGGTGCTCAATCCCGGC 
GGCGTGCGCATCGGCACCGCGGAAATCTACCGCCAGGTGGAAAAGGTCGAGGAAGTCCTCGAGTCCATCGCC 
ATCGGCCAGGACTGGCAGGGCGATGTGCGTGTGCTGCTGTTCGTCCGCCTGCGCGACGGCGTGCGACTCGAC 
GAGCCGCTGCGTGAACGCATCCGCCAGACCATCCGCGCCAACGCCACGCCGCGCCACGTACCGGCGAAGATC 
ATCCAGGTCGCCGATATCCCGCGCACCCTGAGCGGCAAGATCGTCGAGCTTGCGGTGCGCAACGTGATCCAC 
GGCCGCCCGGTGAAGAACACCGACGCCCTGGCCAACCCGCAGGCGCTGGAGCTTTATCGCGACCTCGCCGAA 
CTGCGCGACTGA