ViewGene
Locus Name:
GeneID:
Location:

GeneID:3432
TrivialName: PA14_69720
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_69720
PAO1 Ortholog Locus:PA5281
Sequence Length:699
Protein Length:232
Start: 6218435
Stop: 6219133
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08666.1|AE004940_10 (AE004940) probable hydrolase [Pseudomonas aeruginosa PAO1]
Identities = 228/232 (98%), Positives = 232/232 (100%)
Sequence:
ATGAGCAGCCTGCGACTGATCACCTTCGACCTCGACGACACCCTGTGGGACGTCGCCCCGGTGATGAACAAC
GCCGAGGCCCTGCTCCGCGAATGGCTGGCGAGCAACGCGGCGCGCCTGGGTCCGGTGCCCATCGAGCACCTG
TGGGCGATCCGCACCCGCCTGCTGGATCGCGAACCGATGCTGCGCCACCGTCTCAGCGAGCTGCGCCGGCGC
ATCCTGTTCCATGCCCTGCTCGACGCCGGTTATCCACAGGCGGAGGCGGAAAGCCTGGCCGAGGCCGGTTTC
CAGGTATTCCTCGAAGCGCGCCACCGGGTCACCCTGTTTCCCGAAGTCCACCCGACCCTGGAGATCCTCGCC
GACCGCTTCACCCTCGGCGTGCTCACCAACGGTAACGCCGACGTGCGCCGCCTGGGCCTGGCCGACTACTTC
CGGTTCGCCCTCTGCGCCGAGGAACTGGGCGTCGGCAAGCCCGACCCGACGCCATTCCGCGAAGCCCTGAAG
CGCGCCGGCGTCGAGGCCTCGGCAGCGGTACACATCGGCGACCACCCCAACGACGACATCGCCGGAGCGCGC
CGCGCCGGCATGCGTGCCATCTGGTTCAACCCAAACGGCAAGCCCTGGGCCGGGGAGGAAGAGCCGAGCGCG
GAGATCCGCAGCCTCGCCGAGCTGCCGGCCCTGCTCGCCCGCCTGCAATGA
Translation:
MSSLRLITFDLDDTLWDVAPVMNNAEALLREWLASNAARLGPVPIEHLWAIRTRLLDREPMLRHRLSELRRR
ILFHALLDAGYPQAEAESLAEAGFQVFLEARHRVTLFPEVHPTLEILADRFTLGVLTNGNADVRRLGLADYF
RFALCAEELGVGKPDPTPFREALKRAGVEASAAVHIGDHPNDDIAGARRAGMRAIWFNPNGKPWAGEEEPSA
EIRSLAELPALLARLQ*
AnnotationID:3440GeneID:3432
AnnotatorUID: diggins
Modification Date/Time: 2005-03-16 08:08:43
Gene Name:
Confidence Code:3
GeneProduct:putative hydrolase
Cell Localization:()
Synonyms:PA5281
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1011,pfam00702,COG0546,COG0637
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53767.1| (AE016856) HAD-superfamily hydrolase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 230

 Score =  337 bits (863), Expect = 4e-92
 Identities = 156/227 (68%), Positives = 188/227 (82%)

>gb|AAN70796.1|AE016723_8 (AE016793) hydrolase, haloacid dehalogenase-like family
           [Pseudomonas putida KT2440]
          Length = 231

 Score =  326 bits (836), Expect = 6e-89
 Identities = 152/228 (66%), Positives = 187/228 (82%)

>gb|AAL83558.1|AF414717_8 (AF414717) putative hydrolase [Pseudomonas stutzeri]
          Length = 159

 Score =  213 bits (542), Expect = 7e-55
 Identities = 101/153 (66%), Positives = 120/153 (78%)








 
Structural Features:
COG1011, COG1011, Predicted hydrolase (HAD superfamily)
CD-Length = 229 residues, 100.0% aligned
Score =  129 bits (324), Expect = 4e-31
Identities = 74/237 (31%), Positives = 102/237 (43%), Gaps = 14/237 (5%)

pfam00702, Hydrolase, haloacid dehalogenase-like hydrolase.
CD-Length = 197 residues,  99.0% aligned
Score = 84.6 bits (208), Expect = 9e-18
Identities = 53/204 (25%), Positives = 77/204 (37%), Gaps = 15/204 (7%)

COG0546, Gph, Predicted phosphatases 
CD-Length = 220 residues,  99.1% aligned
Score = 84.4 bits (208), Expect = 1e-17
Identities = 69/239 (28%), Positives = 99/239 (41%), Gaps = 31/239 (12%)

COG0637, COG0637, Predicted phosphatase/phosphohexomutase
CD-Length = 221 residues,  97.3% aligned
Score = 72.1 bits (176), Expect = 6e-14
Identities = 56/236 (23%), Positives = 84/236 (35%), Gaps = 27/236 (11%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6867




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24009




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34786




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4520_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3432
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5484
                           PA5281_tr
                           translation of PA5281
                           232
                           98.27
                           99.99
                           0
                           1187.0
     

     
     
153233
                           gb|AAG08666.1|AE004940_10
                           (AE004940) probable hydrolase [Pseudomonas aeruginosa PAO1]
                           232
                           98.27
                           99.99
                           0
                           1187.0
     

     
     
153234
                           gb|AAO53767.1|
                           (AE016856) HAD-superfamily hydrolase [Pseudomonas syringae pv. tomato str. DC3000]
                           232
                           67.24
                           81.03
                           2
                           859.0
     

     
     
153235
                           gb|AAN70796.1|AE016723_8
                           (AE016793) hydrolase, haloacid dehalogenase-like family [Pseudomonas putida KT2440]
                           232
                           65.51
                           81.03
                           1
                           834.0
     

     
     
153236
                           gb|AAL83558.1|AF414717_8
                           (AF414717) putative hydrolase [Pseudomonas stutzeri]
                           234
                           43.16
                           51.70
                           77
                           539.0
     

     






      

          
GC ORFID: 46434How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5281Glimmer Score: 

          
Start: 5482122Stop: 5482820

          
Length: 699


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5281 translation of PA5281Homolog Bit Score 461.0

          
Other Homologs:  t_PA5281 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAGCCTGCGACTGATCACCTTCGACCTCGACGACACCCTGTGGGACGTCGCCCCGGTGATGAACAAC 
GCCGAGGCCCTGCTCCGCGAATGGCTGGCGAGCAACGCGGCGCGCCTGGGTCCGGTGCCCATCGAGCACCTG 
TGGGCGATCCGCACCCGCCTGCTGGATCGCGAACCGATGCTGCGCCACCGTCTCAGCGAGCTGCGCCGGCGC 
ATCCTGTTCCATGCCCTGCTCGACGCCGGTTATCCACAGGCGGAGGCGGAAAGCCTGGCCGAGGCCGGTTTC 
CAGGTATTCCTCGAAGCGCGCCACCGGGTCACCCTGTTTCCCGAAGTCCACCCGACCCTGGAGATCCTCGCC 
GACCGCTTCACCCTCGGCGTGCTCACCAACGGTAACGCCGACGTGCGCCGCCTGGGCCTGGCCGACTACTTC 
CGGTTCGCCCTCTGCGCCGAGGAACTGGGCGTCGGCAAGCCCGACCCGACGCCATTCCGCGAAGCCCTGAAG 
CGCGCCGGCGTCGAGGCCTCGGCAGCGGTACACATCGGCGACCACCCCAACGACGACATCGCCGGAGCGCGC 
CGCGCCGGCATGCGTGCCATCTGGTTCAACCCAAACGGCAAGCCCTGGGCCGGGGAGGAAGAGCCGAGCGCG 
GAGATCCGCAGCCTCGCCGAGCTGCCGGCCCTGCTCGCCCGCCTGCAATGA