ViewGene
Locus Name:
GeneID:
Location:

GeneID:3405
TrivialName: PA14_25860
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25860
PAO1 Ortholog Locus:PA2952
Sequence Length:750
Protein Length:249
Start: 2256891
Stop: 2257640
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06340.1|AE004721_8 (AE004721) electron transfer flavoprotein beta-subunit [Pseudomonas aeruginosa PAO1]
Identities = 249/249 (100%), Positives = 249/249 (100%)
Sequence:
ATGAAGGTTCTTGTAGCAGTCAAACGAGTGGTCGACTACAACGTCAAGGTCCGCGTCAAGGCGGACAACTCC
GGCGTCGACCTCGCCAACGTCAAGATGTCCATGAACCCCTTCTGCGAAATCGCCGTGGAAGAAGCCGTACGC
CTGAAAGAGAAGGGCGTGGCTACCGAGATCGTCGCTGTCTCCGTCGGCCCGACCGCCGCCCAGGAGCAACTG
CGCACCGCGCTGGCGCTGGGCGCTGACCGTGCGATCCTGGTGGAGAGCAACGACGAGCTGAACTCCCTGGCC
GTGGCCAAGCTGCTCAAGGCCGTGGTCGACAAGGAGCAGCCGCAGCTGGTGATCCTCGGCAAGCAGGCCATC
GACAGCGACAACAACCAGACCGGCCAGATGCTCGCCGCGCTGACCGGCTACGCCCAGGGCACCTTCGCCTCC
AAGGTCGAGGTCGCTGGCGACAAGGTCAACGTGACCCGCGAGATCGATGGCGGTCTGCAGACCGTCGCGCTG
AACCTGCCGGCCATCGTCACCACCGACCTGCGCCTGAACGAGCCGCGCTACGCGTCGCTGCCGAACATCATG
AAAGCCAAGAAGAAGCCGCTGGACGTGGTGACCCCGGACGCCCTGGGCGTTTCCACCGCTTCCACCGTGAAG
ACCCTGAAGGTCGAGGCGCCGGCTGCGCGCTCCGCTGGCATCAAGGTCAAGTCCGTGGCTGAACTGGTAGAG
AAACTGAAGAACGAGGCGAAGGTAATCTAA
Translation:
MKVLVAVKRVVDYNVKVRVKADNSGVDLANVKMSMNPFCEIAVEEAVRLKEKGVATEIVAVSVGPTAAQEQL
RTALALGADRAILVESNDELNSLAVAKLLKAVVDKEQPQLVILGKQAIDSDNNQTGQMLAALTGYAQGTFAS
KVEVAGDKVNVTREIDGGLQTVALNLPAIVTTDLRLNEPRYASLPNIMKAKKKPLDVVTPDALGVSTASTVK
TLKVEAPAARSAGIKVKSVAELVEKLKNEAKVI*
AnnotationID:3413GeneID:3405
AnnotatorUID: diggins
Modification Date/Time: 2005-04-08 17:05:55
Gene Name:etfB
Confidence Code:2
GeneProduct:electron transfer flavoprotein beta-subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2086,pfam01012
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69783.1|AE016614_9 (AE016789) electron transfer flavoprotein, beta subunit
           [Pseudomonas putida KT2440]
          Length = 249

 Score =  444 bits (1141), Expect = e-124
 Identities = 237/249 (95%), Positives = 242/249 (97%)

>gb|AAO55701.1| (AE016863) electron transfer flavoprotein, beta subunit
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 249

 Score =  434 bits (1117), Expect = e-121
 Identities = 231/249 (92%), Positives = 240/249 (96%)

>emb|CAE31120.1| (BX640438) electron transfer flavoprotein beta-subunit [Bordetella
           bronchiseptica]
          Length = 251

 Score =  357 bits (917), Expect = 3e-98
 Identities = 186/249 (74%), Positives = 212/249 (85%)








 
Structural Features:
COG2086, FixA, Electron transfer flavoprotein, beta subunit 
[Energy production and conversion]
CD-Length = 260 residues, 100.0% aligned
Score =  251 bits (642), Expect = 7e-68
Identities = 131/260 (50%), Positives = 167/260 (64%), Gaps = 11/260 (4%)

pfam01012, ETF_beta, Electron transfer flavoprotein beta subunit. 
CD-Length = 255 residues, 100.0% aligned
Score =  244 bits (623), Expect = 1e-65
Identities = 122/255 (47%), Positives = 157/255 (61%), Gaps = 6/255 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:6813




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23955




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36098




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3405_cdd.html


    		




 
URL:

















    ReferenceID:36429




  
Author/Investigator(s):
        Boynton ZL, Bennet GN, Rudolph FB.





  
Title:
        Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824.




  
PubMed:
        8655474


MedLine:
        




    
Source:
J Bacteriol. 1996 Jun;178(11):3015-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8655474









           
Homologs By Global Alignment 

           

       



     
Gene ID:3405
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5440
                           PA2952_tr
                           translation of PA2952
                           249
                           99.99
                           99.99
                           0
                           1196.0
     

     
     
151656
                           gb|AAG06340.1|AE004721_8
                           (AE004721) electron transfer flavoprotein beta-subunit [Pseudomonas aeruginosa PAO1]
                           249
                           99.99
                           99.99
                           0
                           1196.0
     

     
     
151657
                           gb|AAN69783.1|AE016614_9
                           (AE016789) electron transfer flavoprotein, beta subunit [Pseudomonas putida KT2440]
                           249
                           95.18
                           97.18
                           0
                           1141.0
     

     
     
151658
                           gb|AAO55701.1|
                           (AE016863) electron transfer flavoprotein, beta subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           249
                           92.77
                           96.38
                           0
                           1117.0
     

     
     
151667
                           gb|AAQ61480.1|
                           (AE016923) probable electron transfer flavoprotein, beta subunit [Chromobacterium violaceum ATCC 12472]
                           249
                           74.69
                           82.73
                           0
                           886.0
     

     






      

          
GC ORFID: 46407How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2952Glimmer Score: 

          
Start: 2697034Stop: 2696285

          
Length: 750


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2952 translation of PA2952Homolog Bit Score 465.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene980 (ORF: BLASTX 442.0), psyr_15may02_Scaffold1_revised_gene294 (ORF: BLASTX 429.0), t_PA2952 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGGTTCTTGTAGCAGTCAAACGAGTGGTCGACTACAACGTCAAGGTCCGCGTCAAGGCGGACAACTCC 
GGCGTCGACCTCGCCAACGTCAAGATGTCCATGAACCCCTTCTGCGAAATCGCCGTGGAAGAAGCCGTACGC 
CTGAAAGAGAAGGGCGTGGCTACCGAGATCGTCGCTGTCTCCGTCGGCCCGACCGCCGCCCAGGAGCAACTG 
CGCACCGCGCTGGCGCTGGGCGCTGACCGTGCGATCCTGGTGGAGAGCAACGACGAGCTGAACTCCCTGGCC 
GTGGCCAAGCTGCTCAAGGCCGTGGTCGACAAGGAGCAGCCGCAGCTGGTGATCCTCGGCAAGCAGGCCATC 
GACAGCGACAACAACCAGACCGGCCAGATGCTCGCCGCGCTGACCGGCTACGCCCAGGGCACCTTCGCCTCC 
AAGGTCGAGGTCGCTGGCGACAAGGTCAACGTGACCCGCGAGATCGATGGCGGTCTGCAGACCGTCGCGCTG 
AACCTGCCGGCCATCGTCACCACCGACCTGCGCCTGAACGAGCCGCGCTACGCGTCGCTGCCGAACATCATG 
AAAGCCAAGAAGAAGCCGCTGGACGTGGTGACCCCGGACGCCCTGGGCGTTTCCACCGCTTCCACCGTGAAG 
ACCCTGAAGGTCGAGGCGCCGGCTGCGCGCTCCGCTGGCATCAAGGTCAAGTCCGTGGCTGAACTGGTAGAG 
AAACTGAAGAACGAGGCGAAGGTAATCTAA