ViewGene
Locus Name:
GeneID:
Location:

GeneID:340
TrivialName: PA14_69850
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_69850
PAO1 Ortholog Locus:PA5291
Sequence Length:1986
Protein Length:661
Start: 6228394
Stop: 6230379
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08676.1|AE004941_9 (AE004941) probable choline transporter [Pseudomonas aeruginosa PAO1]
Identities = 661/661 (100%), Positives = 661/661 (100%)
Sequence:
ATGGAGACACCCACTACCCCCCGGAGCACCCTGAATCCTCCGGTCTTCTATACCTCCGCCGCCCTGATCCTG
GCCCTGGTGCTGTTCACCGTGCTCCTCCAGGAAGAAGCGCAATCGCTGTTCAACCACGTCCAGCAATGGATC
ATCACCAACGCCAGCTGGTTCTACGTACTGGCCGTGGCGCTGATCCTGATCAGCGTGGTGTTCCTCGCCGTC
AGCCGCTACGGCGACATCAAGCTCGGCCCCGACCACAGTCGTCCCGACTACCGCAACACCACCTGGTTCGCC
ATGCTGTTCTCCGCCGGCATGGGCATCGGTCTGATGTTCTTCGGCGTTGCCGAACCGGTGATGCACTTCACC
ACCCCACCGGTAGGCGAGCCGAACACCGTGCAGGCGGCGAAGGAAGCGATGAAGCTGACCTTCTTCCACTGG
GGCCTGCATGCCTGGGCGATCTACGCCATCGTCGCGCTGATCCTGGCCTTCTTCAGCTATCGCCACGGCCTG
CCGCTGACCCTGCGCTCGGCGCTGTATCCGCTGATCGGCGAACGTATCTACGGCCCCATCGGCCACGCGGTG
GATATCTTCGCGATCATCGGCACCGTGTTCGGCGTGGCGACTTCGCTCGGCTACGGCGTGCTGCAGATCAAC
AGCGGCCTGCACCACCTGTTCGGCTGGCCGGTGAACCAGACCGTGCAGATCGCCCTGATCGCCGCCACCTGC
GGCCTCGCCACGCTCTCGGTGGCCAGCGGACTGGACCGCGGCATCCGCATCCTGTCCGAGCTGAACCTGAGC
CTGGCGGTGATCCTGCTGCTCTTCGTGCTGGTCCTCGGGCCGACCGTGTTCCTCCTGCAAACCTACGTGCAG
AACACCGGGGCCTACCTGTCCGACATCGTCAACAAGACCTTCAACCTGTACGCCTACGAGCCCACCGACTGG
ATCGGCGGCTGGACCCTGCTGTACTGGGGCTGGTGGCTGTCCTGGTCGCCCTTCGTCGGCCTGTTCATCGCC
CGTATCTCGCGCGGCCGCACGATCCGCGAGTTCGTCTGCGGCGTGCTGTTCGTCCCGGCCGGCTTCACCCTG
CTGTGGATGACGGTGTTCGGCGATACCGCCATCCACATGGTCCTGCAGGAGGGCTTCACCCAGTTGGCCGAA
GTGGTCAACCAGGACAGTTCGGTGGCGCTGTTCGCCTTCCTCGAGCACTTCCCGCTGGGCAGCGTGATTTCG
CTGGTGGCGGTGCTGATGGTGGTGGTGTTCTTCGTCACCTCGGCCGACTCCGGCGCGCTGGTGGTGGACATG
CTGGCCTCCAGCGGCAAGGACCACTCGCCGCTCTGGCAGCGGATATTTTGGTCGGTGCTGATGGGCGTGGTG
GCCATCGCCCTGCTCCTCGCCGATGGCCTCAAGGCGCTGCAAACCGCGACCATCGCCAGCGCGCTGCCATTC
TCCATCGTGCTCCTGGCGTCGATCTGGGGCCTGTTCAAGGCGCTGCACCTGGATGCCACCAAGCGCGGCATC
CGCTACCAGGCGCTGACCTTCTCCCGCCCCACCACGCGCAGCGGCGCAAGCGAGGGCGACGGTTGGCAGCGC
CGCTTGCGCAACATCGCCATGTTCCCGAGGCGCAGCCACGTCAACCGCTTCATCACCGATGTGGCGCGACCG
GCCTGCGAGGACGTCGCCGCCGAACTGCGCAAGCAGGGCTACGAAGTCCAGGTGCAGGACGGCGAGGACGGC
CGGGTTCGGCTGGAAGTCAGCCACGGCGGCGAGGTCGATTTCATCTACGAGGTGCGTCCGCGCGCCTTCACC
ACGCCGAGCTTCGTGATGCGCGACACCGAGGACAGCAGCGAGTCGCGCAAGTATTTCCGCGCCGAGGTCCAT
CTCCAGGAAGGCGGCCAGGACTACGACATCATGGGCTGGAGCCGCGACGGCGTGATCGGCGACATCCTCGAT
CAGTACGAGCGGCACATGCACTTCCTGCACGTGGTGCGCTGA
Translation:
METPTTPRSTLNPPVFYTSAALILALVLFTVLLQEEAQSLFNHVQQWIITNASWFYVLAVALILISVVFLAV
SRYGDIKLGPDHSRPDYRNTTWFAMLFSAGMGIGLMFFGVAEPVMHFTTPPVGEPNTVQAAKEAMKLTFFHW
GLHAWAIYAIVALILAFFSYRHGLPLTLRSALYPLIGERIYGPIGHAVDIFAIIGTVFGVATSLGYGVLQIN
SGLHHLFGWPVNQTVQIALIAATCGLATLSVASGLDRGIRILSELNLSLAVILLLFVLVLGPTVFLLQTYVQ
NTGAYLSDIVNKTFNLYAYEPTDWIGGWTLLYWGWWLSWSPFVGLFIARISRGRTIREFVCGVLFVPAGFTL
LWMTVFGDTAIHMVLQEGFTQLAEVVNQDSSVALFAFLEHFPLGSVISLVAVLMVVVFFVTSADSGALVVDM
LASSGKDHSPLWQRIFWSVLMGVVAIALLLADGLKALQTATIASALPFSIVLLASIWGLFKALHLDATKRGI
RYQALTFSRPTTRSGASEGDGWQRRLRNIAMFPRRSHVNRFITDVARPACEDVAAELRKQGYEVQVQDGEDG
RVRLEVSHGGEVDFIYEVRPRAFTTPSFVMRDTEDSSESRKYFRAEVHLQEGGQDYDIMGWSRDGVIGDILD
QYERHMHFLHVVR*
AnnotationID:349GeneID:340
AnnotatorUID: diggins
Modification Date/Time: 2005-03-16 09:09:13
Gene Name:
Confidence Code:3
GeneProduct:putative holine-glycine betaine transporter
Cell Localization:()
Synonyms:betT
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1292,pfam02028
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69551.1|AE016588_8 (AE016788) choline/carnitine/betaine transporter family protein
           [Pseudomonas putida KT2440]
          Length = 657

 Score = 1106 bits (2861), Expect = 0.0
 Identities = 542/660 (82%), Positives = 592/660 (89%), Gaps = 4/660 (0%)

>emb|CAE35824.1| (BX640448) high-affinity choline transport protein [Bordetella
           bronchiseptica]
          Length = 652

 Score =  987 bits (2551), Expect = 0.0
 Identities = 488/653 (74%), Positives = 546/653 (83%), Gaps = 7/653 (1%)

>emb|CAE41134.1| (BX640413) high-affinity choline transport protein [Bordetella
           pertussis]
          Length = 652

 Score =  987 bits (2551), Expect = 0.0
 Identities = 488/653 (74%), Positives = 546/653 (83%), Gaps = 7/653 (1%)








 
Structural Features:
COG1292, BetT, Choline-glycine betaine transporter 
[Cell envelope biogenesis, outer membrane]
CD-Length = 537 residues, 100.0% aligned
Score =  637 bits (1645), Expect = 0.0
Identities = 292/561 (52%), Positives = 382/561 (68%), Gaps = 25/561 (4%)

pfam02028, BCCT, BCCT family transporter.
CD-Length = 487 residues, 100.0% aligned
Score =  522 bits (1345), Expect = 6e-149
Identities = 244/488 (50%), Positives = 340/488 (69%), Gaps = 5/488 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:683




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17825




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34789




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:340_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:340
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
559
                           PA5291_tr
                           translation of PA5291
                           661
                           99.99
                           99.99
                           0
                           3422.0
     

     
     
27201
                           gb|AAG08676.1|AE004941_9
                           (AE004941) probable choline transporter [Pseudomonas aeruginosa PAO1]
                           661
                           99.99
                           99.99
                           0
                           3422.0
     

     
     
27202
                           gb|AAN69551.1|AE016588_8
                           (AE016788) choline/carnitine/betaine transporter family protein [Pseudomonas putida KT2440]
                           661
                           81.99
                           89.56
                           4
                           2862.0
     

     
     
27205
                           emb|CAE38685.1|
                           (BX640433) high-affinity choline transport protein [Bordetella parapertussis]
                           661
                           74.28
                           82.90
                           9
                           2554.0
     

     
     
27203
                           emb|CAE35824.1|
                           (BX640448) high-affinity choline transport protein [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           661
                           74.13
                           82.90
                           9
                           2557.0
     

     






      

          
GC ORFID: 43342How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5291Glimmer Score: 

          
Start: 5492082Stop: 5494067

          
Length: 1986


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5291 translation of PA5291Homolog Bit Score 1322.0

          
Other Homologs:  t_PA3933 (ORF: BLASTX 638.0), psyr_15may02_Scaffold6_revised_gene6540 (ORF: BLASTX 621.0), t_PA5291 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGAGACACCCACTACCCCCCGGAGCACCCTGAATCCTCCGGTCTTCTATACCTCCGCCGCCCTGATCCTG 
GCCCTGGTGCTGTTCACCGTGCTCCTCCAGGAAGAAGCGCAATCGCTGTTCAACCACGTCCAGCAATGGATC 
ATCACCAACGCCAGCTGGTTCTACGTACTGGCCGTGGCGCTGATCCTGATCAGCGTGGTGTTCCTCGCCGTC 
AGCCGCTACGGCGACATCAAGCTCGGCCCCGACCACAGTCGTCCCGACTACCGCAACACCACCTGGTTCGCC 
ATGCTGTTCTCCGCCGGCATGGGCATCGGTCTGATGTTCTTCGGCGTTGCCGAACCGGTGATGCACTTCACC 
ACCCCACCGGTAGGCGAGCCGAACACCGTGCAGGCGGCGAAGGAAGCGATGAAGCTGACCTTCTTCCACTGG 
GGCCTGCATGCCTGGGCGATCTACGCCATCGTCGCGCTGATCCTGGCCTTCTTCAGCTATCGCCACGGCCTG 
CCGCTGACCCTGCGCTCGGCGCTGTATCCGCTGATCGGCGAACGTATCTACGGCCCCATCGGCCACGCGGTG 
GATATCTTCGCGATCATCGGCACCGTGTTCGGCGTGGCGACTTCGCTCGGCTACGGCGTGCTGCAGATCAAC 
AGCGGCCTGCACCACCTGTTCGGCTGGCCGGTGAACCAGACCGTGCAGATCGCCCTGATCGCCGCCACCTGC 
GGCCTCGCCACGCTCTCGGTGGCCAGCGGACTGGACCGCGGCATCCGCATCCTGTCCGAGCTGAACCTGAGC 
CTGGCGGTGATCCTGCTGCTCTTCGTGCTGGTCCTCGGGCCGACCGTGTTCCTCCTGCAAACCTACGTGCAG 
AACACCGGGGCCTACCTGTCCGACATCGTCAACAAGACCTTCAACCTGTACGCCTACGAGCCCACCGACTGG 
ATCGGCGGCTGGACCCTGCTGTACTGGGGCTGGTGGCTGTCCTGGTCGCCCTTCGTCGGCCTGTTCATCGCC 
CGTATCTCGCGCGGCCGCACGATCCGCGAGTTCGTCTGCGGCGTGCTGTTCGTCCCGGCCGGCTTCACCCTG 
CTGTGGATGACGGTGTTCGGCGATACCGCCATCCACATGGTCCTGCAGGAGGGCTTCACCCAGTTGGCCGAA 
GTGGTCAACCAGGACAGTTCGGTGGCGCTGTTCGCCTTCCTCGAGCACTTCCCGCTGGGCAGCGTGATTTCG 
CTGGTGGCGGTGCTGATGGTGGTGGTGTTCTTCGTCACCTCGGCCGACTCCGGCGCGCTGGTGGTGGACATG 
CTGGCCTCCAGCGGCAAGGACCACTCGCCGCTCTGGCAGCGGATATTTTGGTCGGTGCTGATGGGCGTGGTG 
GCCATCGCCCTGCTCCTCGCCGATGGCCTCAAGGCGCTGCAAACCGCGACCATCGCCAGCGCGCTGCCATTC 
TCCATCGTGCTCCTGGCGTCGATCTGGGGCCTGTTCAAGGCGCTGCACCTGGATGCCACCAAGCGCGGCATC 
CGCTACCAGGCGCTGACCTTCTCCCGCCCCACCACGCGCAGCGGCGCAAGCGAGGGCGACGGTTGGCAGCGC 
CGCTTGCGCAACATCGCCATGTTCCCGAGGCGCAGCCACGTCAACCGCTTCATCACCGATGTGGCGCGACCG 
GCCTGCGAGGACGTCGCCGCCGAACTGCGCAAGCAGGGCTACGAAGTCCAGGTGCAGGACGGCGAGGACGGC 
CGGGTTCGGCTGGAAGTCAGCCACGGCGGCGAGGTCGATTTCATCTACGAGGTGCGTCCGCGCGCCTTCACC 
ACGCCGAGCTTCGTGATGCGCGACACCGAGGACAGCAGCGAGTCGCGCAAGTATTTCCGCGCCGAGGTCCAT 
CTCCAGGAAGGCGGCCAGGACTACGACATCATGGGCTGGAGCCGCGACGGCGTGATCGGCGACATCCTCGAT 
CAGTACGAGCGGCACATGCACTTCCTGCACGTGGTGCGCTGA