ViewGene
Locus Name:
GeneID:
Location:

GeneID:3392
TrivialName: PA14_47650
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_47650
PAO1 Ortholog Locus:PA1281
Sequence Length:738
Protein Length:245
Start: 4242732
Stop: 4241995
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04670.1|AE004557_11 (AE004557) cobalamin (5'-phosphate) synthase [Pseudomonas aeruginosa PAO1]
Identities = 244/245 (99%), Positives = 244/245 (99%)
Sequence:
ATGCGCGAAGCCTTGCGCTCACTGCTGGTCGCCTTGCAGTTCCTCACCCGCCTGCCGGTGCGCCTGTCGGCG
ATGCCGACGCCGGAACAGTTCGGCCGCGCGGTGCTCTGCTATCCACTGGTCGGGGTGCTGATCGGCGTTGTG
CTGTACGCCGCGGCGCGGTCGCTCGACGGTACGCCGCCGCTGTTGCAGGCAGCCTTGCTGCTGAGCCTGTGG
GTGGCGCTGAGCGGCGCGCTGCACCTGGACGGCCTGGCCGACATGGCGGACGCCTGGGTCGGCGGGCTGGGC
GACCGCGAGCGGACCCTGGCGATCATGAAGGACCCGCGCAGCGGCCCGGTGGCGGTGGTGGTCCTGGTACTG
GTCCTGTTGTTGAAGTTCTCCGCCCTGGCGGCGCTGCTCGGCCAGGGAGAGGCCGGGTTGTTGCCGCTGGCC
CCGTGGCTGGCGCGCAGCAGCCTGCCGCTGCTGTTCCTGACCACGCCCTACGCGCGCCCCGGCGGCCTCGGC
CAGGCCATCGCCGAGCACCTGCCGGCGCGCAGCCTGCCGTGGGTGCTGGGGGGGAGTTTCGGCCTGGCCCTG
GCGTTCGGCCTGGCCGGCCTGCTGGCGTTGCTGGTGACGCTGATGCTCTTCGCCTGGCTGCGCAGCCGTTTC
CTCGCGCGCCTCGGTGGCACCACCGGGGACACCGCCGGCGCGCTGGTCGAACTTACCGAGTGTGCGGTGCTG
GTGGCGCTGGCTCTGTAG
Translation:
MREALRSLLVALQFLTRLPVRLSAMPTPEQFGRAVLCYPLVGVLIGVVLYAAARSLDGTPPLLQAALLLSLW
VALSGALHLDGLADMADAWVGGLGDRERTLAIMKDPRSGPVAVVVLVLVLLLKFSALAALLGQGEAGLLPLA
PWLARSSLPLLFLTTPYARPGGLGQAIAEHLPARSLPWVLGGSFGLALAFGLAGLLALLVTLMLFAWLRSRF
LARLGGTTGDTAGALVELTECAVLVALAL*
AnnotationID:3400GeneID:3392
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 08:08:07
Gene Name:cobV
Confidence Code:2
GeneProduct:cobalamin (5'-phosphate) synthase
Cell Localization:()
Synonyms:cobS
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0368
Secondary Functional Category(ies):coenzyme metabolism
EC Number:2.-.-.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67302.1|AE016356_10 (AE016780) cobalamin (5'-phosphate) synthase [Pseudomonas putida
           KT2440]
          Length = 251

 Score =  274 bits (700), Expect = 4e-73
 Identities = 146/236 (61%), Positives = 174/236 (73%), Gaps = 3/236 (1%)

>emb|CAD44477.1| (AJ297529) cobalamin (5' phosphate) synthase [Pseudomonas stutzeri]
          Length = 245

 Score =  274 bits (700), Expect = 4e-73
 Identities = 150/238 (63%), Positives = 173/238 (72%)

>gb|AAO55237.1| (AE016861) cobalamin (5'-phosphate) synthase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 243

 Score =  256 bits (653), Expect = 1e-67
 Identities = 140/238 (58%), Positives = 170/238 (71%), Gaps = 3/238 (1%)






 
Structural Features:
COG0368, CobS, Cobalamin-5-phosphate synthase [Coenzyme metabolism]
CD-Length = 246 residues,  97.6% aligned
Score =  138 bits (348), Expect = 8e-34
Identities = 112/244 (45%), Positives = 140/244 (57%), Gaps = 7/244 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6787




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23929




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37501




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3392_cdd.html


    		




 
URL:

















    ReferenceID:38021




  
Author/Investigator(s):
        Cameron B, Blanche F, Rouyez MC, Bisch D, Famechon A, Couder M, Cauchois L, Thibaut D, Debussche L, Crouzet J.





  
Title:
        Genetic analysis, nucleotide sequence, and products of two Pseudomonas denitrificans cob genes encoding nicotinate-nucleotide: dimethylbenzimidazole phosphoribosyltransferase and cobalamin (5'-phosphate) synthase.




  
PubMed:
        1917841


MedLine:
        




    
Source:
J Bacteriol. 1991 Oct;173(19):6066-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1917841

















    ReferenceID:38024




  
Author/Investigator(s):
        Cameron B, Guilhot C, Blanche F, Cauchois L, Rouyez MC, Rigault S, Levy-Schil S, Crouzet J.





  
Title:
        Genetic and sequence analyses of a Pseudomonas denitrificans DNA fragment containing two cob genes.




  
PubMed:
        1917840


MedLine:
        




    
Source:
J Bacteriol. 1991 Oct;173(19):6058-65.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1917840









           
Homologs By Global Alignment 

           

       



     
Gene ID:3392
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5424
                           PA1281_tr
                           translation of PA1281
                           245
                           99.59
                           99.59
                           0
                           1203.0
     

     
     
151445
                           gb|AAG04670.1|AE004557_11
                           (AE004557) cobalamin (5'-phosphate) synthase [Pseudomonas aeruginosa PAO1]
                           245
                           99.59
                           99.59
                           0
                           1203.0
     

     
     
151447
                           emb|CAD44477.1|
                           (AJ297529) cobalamin (5' phosphate) synthase [Pseudomonas stutzeri]
                           249
                           60.24
                           69.87
                           8
                           699.0
     

     
     
151446
                           gb|AAN67302.1|AE016356_10
                           (AE016780) cobalamin (5'-phosphate) synthase [Pseudomonas putida KT2440]
                           255
                           58.03
                           70.19
                           14
                           703.0
     

     
     
151448
                           gb|AAO55237.1|
                           (AE016861) cobalamin (5'-phosphate) synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           249
                           57.02
                           69.07
                           10
                           657.0
     

     






      

          
GC ORFID: 46394How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1281Glimmer Score: 

          
Start: 711177Stop: 711914

          
Length: 738


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA1281 translation of PA1281Homolog Bit Score 468.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1367 (ORF: BLASTX 253.0), t_PA1281 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCGAAGCCTTGCGCTCACTGCTGGTCGCCTTGCAGTTCCTCACCCGCCTGCCGGTGCGCCTGTCGGCG 
ATGCCGACGCCGGAACAGTTCGGCCGCGCGGTGCTCTGCTATCCACTGGTCGGGGTGCTGATCGGCGTTGTG 
CTGTACGCCGCGGCGCGGTCGCTCGACGGTACGCCGCCGCTGTTGCAGGCAGCCTTGCTGCTGAGCCTGTGG 
GTGGCGCTGAGCGGCGCGCTGCACCTGGACGGCCTGGCCGACATGGCGGACGCCTGGGTCGGCGGGCTGGGC 
GACCGCGAGCGGACCCTGGCGATCATGAAGGACCCGCGCAGCGGCCCGGTGGCGGTGGTGGTCCTGGTACTG 
GTCCTGTTGTTGAAGTTCTCCGCCCTGGCGGCGCTGCTCGGCCAGGGAGAGGCCGGGTTGTTGCCGCTGGCC 
CCGTGGCTGGCGCGCAGCAGCCTGCCGCTGCTGTTCCTGACCACGCCCTACGCGCGCCCCGGCGGCCTCGGC 
CAGGCCATCGCCGAGCACCTGCCGGCGCGCAGCCTGCCGTGGGTGCTGGGGGGGAGTTTCGGCCTGGCCCTG 
GCGTTCGGCCTGGCCGGCCTGCTGGCGTTGCTGGTGACGCTGATGCTCTTCGCCTGGCTGCGCAGCCGTTTC 
CTCGCGCGCCTCGGTGGCACCACCGGGGACACCGCCGGCGCGCTGGTCGAACTTACCGAGTGTGCGGTGCTG 
GTGGCGCTGGCTCTGTAG