ViewGene
Locus Name:
GeneID:
Location:

GeneID:3390
TrivialName: PA14_02300
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02300
PAO1 Ortholog Locus:PA0182
Sequence Length:753
Protein Length:250
Start: 205932
Stop: 206684
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03572.1|AE004456_3 (AE004456) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 249/250 (99%), Positives = 249/250 (99%)
Sequence:
ATGTCCGTATCCTCTGCATCCCTCGCCGGCAAAGTCGCCTTCGTCCAAGGCGGTTCCCGCGGCATCGGCGCC
GCCATCGTTCGCCGGCTGGCCGCCGAAGGCGCCAGCGTCGCCTTCACCTACGTCAGCTCGGCGGACAAGTCC
CGGGCCCTGGCGGCCGAACTCGAAGGCGCCGGAGCCAGCATCCTGGCCCTGCATGCCGATAGCGCCGACGCG
GCGGCGCTCGTCGCCGCCGTCGACGAAGCGGCGGCTCGCTTCGGACGCATCGACATCCTGGTCAACAACGCC
GCTGTCCTCGCCCTCGGTTCGGTGGAAGAGCTGCCGCTGGCCGACTTCGAGCGGACCCTGGCGATCAACGTG
CGCAGCGTCTTCGTCGCCACCCAGGCGGCGGTCAAGCACATGGGCGACGGCGGGCGGGTGATCAGCATCGGC
AGCACCAATGCCGAACGCATGCCGTTCGCCGGCGGCGCCACCTACGCGATGAGCAAGTCGGCGCTGGTCGGC
CTGACCAAGGGCCTGGCCCGCGACCTCGGCCCGCGCGGGATCACGGTGAACAACGTGCAGCCGGGTCCGGTG
GATACCGACATGAACCCGGACGACGGCGACTTCGCCGAGACCCTCAAGGGCCTGATGGCGCTGCCGCGCTAC
GCCCGCAGCGAGGAAATCGCCAGCTTCGTCGCCTACCTGGCCGGGCCGGAAGCGGCCTACATCACCGGGGCC
AGCCTGACCATCGATGGCGGTTTCTCGGCCTGA
Translation:
MSVSSASLAGKVAFVQGGSRGIGAAIVRRLAAEGASVAFTYVSSADKSRALAAELEGAGASILALHADSADA
AALVAAVDEAAARFGRIDILVNNAAVLALGSVEELPLADFERTLAINVRSVFVATQAAVKHMGDGGRVISIG
STNAERMPFAGGATYAMSKSALVGLTKGLARDLGPRGITVNNVQPGPVDTDMNPDDGDFAETLKGLMALPRY
ARSEEIASFVAYLAGPEAAYITGASLTIDGGFSA*
AnnotationID:3398GeneID:3390
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 09:09:07
Gene Name:
Confidence Code:3
GeneProduct:putative short-chain dehydrogenase
Cell Localization:()
Synonyms:PA0182
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00106,COG1028,COG4221
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67471.1|AE016374_3 (AE016780) oxidoreductase, short-chain dehydrogenase/reductase
           family [Pseudomonas putida KT2440]
          Length = 249

 Score =  344 bits (882), Expect = 3e-94
 Identities = 179/244 (73%), Positives = 201/244 (82%)

>gb|AAO55615.1| (AE016863) oxidoreductase, short chain dehydrogenase/reductase
           family [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 249

 Score =  343 bits (879), Expect = 7e-94
 Identities = 174/248 (70%), Positives = 201/248 (81%)

>gb|AAN55833.1|AE015720_5 (AE015720) oxidoreductase, short chain dehydrogenase/reductase
           family [Shewanella oneidensis MR-1]
          Length = 254

 Score =  318 bits (814), Expect = 2e-86
 Identities = 163/247 (65%), Positives = 194/247 (78%)








 
Structural Features:
pfam00106, adh_short, short chain dehydrogenase. 
CD-Length = 247 residues, 100.0% aligned
Score =  174 bits (442), Expect = 1e-44
Identities = 102/249 (40%), Positives = 134/249 (53%), Gaps = 15/249 (6%)

COG1028, FabG, Dehydrogenases with different specificities 
(related to short-chain alcohol dehydrogenases) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 251 residues,  99.6% aligned
Score =  149 bits (377), Expect = 2e-37
Identities = 105/252 (41%), Positives = 138/252 (54%), Gaps = 11/252 (4%)

COG4221, COG4221, Short-chain alcohol dehydrogenase of unknown specificity
CD-Length = 246 residues,  91.9% aligned
Score =  129 bits (325), Expect = 4e-31
Identities = 82/231 (35%), Positives = 116/231 (50%), Gaps = 10/231 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6783




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23925




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33545




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3390_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3390
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5422
                           PA0182_tr
                           translation of PA0182
                           250
                           99.60
                           99.60
                           0
                           1208.0
     

     
     
151376
                           gb|AAG03572.1|AE004456_3
                           (AE004456) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           250
                           99.60
                           99.60
                           0
                           1208.0
     

     
     
151377
                           gb|AAN67471.1|AE016374_3
                           (AE016780) oxidoreductase, short-chain dehydrogenase/reductase family [Pseudomonas putida KT2440]
                           250
                           72.40
                           81.20
                           1
                           880.0
     

     
     
151378
                           gb|AAO55615.1|
                           (AE016863) oxidoreductase, short chain dehydrogenase/reductase family [Pseudomonas syringae pv. tomato str. DC3000]
                           250
                           69.60
                           80.40
                           1
                           878.0
     

     
     
151379
                           gb|AAN55833.1|AE015720_5
                           (AE015720) oxidoreductase, short chain dehydrogenase/reductase family [Shewanella oneidensis MR-1]
                           254
                           64.17
                           76.37
                           4
                           810.0
     

     






      

          
GC ORFID: 46392How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0182Glimmer Score: 

          
Start: 6007271Stop: 6008023

          
Length: 753


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0182 translation of PA0182Homolog Bit Score 469.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene527 (ORF: BLASTX 343.0), t_PA0182 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCGTATCCTCTGCATCCCTCGCCGGCAAAGTCGCCTTCGTCCAAGGCGGTTCCCGCGGCATCGGCGCC 
GCCATCGTTCGCCGGCTGGCCGCCGAAGGCGCCAGCGTCGCCTTCACCTACGTCAGCTCGGCGGACAAGTCC 
CGGGCCCTGGCGGCCGAACTCGAAGGCGCCGGAGCCAGCATCCTGGCCCTGCATGCCGATAGCGCCGACGCG 
GCGGCGCTCGTCGCCGCCGTCGACGAAGCGGCGGCTCGCTTCGGACGCATCGACATCCTGGTCAACAACGCC 
GCTGTCCTCGCCCTCGGTTCGGTGGAAGAGCTGCCGCTGGCCGACTTCGAGCGGACCCTGGCGATCAACGTG 
CGCAGCGTCTTCGTCGCCACCCAGGCGGCGGTCAAGCACATGGGCGACGGCGGGCGGGTGATCAGCATCGGC 
AGCACCAATGCCGAACGCATGCCGTTCGCCGGCGGCGCCACCTACGCGATGAGCAAGTCGGCGCTGGTCGGC 
CTGACCAAGGGCCTGGCCCGCGACCTCGGCCCGCGCGGGATCACGGTGAACAACGTGCAGCCGGGTCCGGTG 
GATACCGACATGAACCCGGACGACGGCGACTTCGCCGAGACCCTCAAGGGCCTGATGGCGCTGCCGCGCTAC 
GCCCGCAGCGAGGAAATCGCCAGCTTCGTCGCCTACCTGGCCGGGCCGGAAGCGGCCTACATCACCGGGGCC 
AGCCTGACCATCGATGGCGGTTTCTCGGCCTGA