ViewGene

Locus Name:
GeneID:
Location:

GeneID: 339

TrivialName: PA14_51530

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_51530

PAO1 Ortholog Locus:

Sequence Length: 2064

Protein Length: 687

Start: 4583020

Stop: 4580957

Strand: -

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAP82959.1| (AY273870) type III effector protein [Pseudomonas aeruginosa] Identities = 687/687 (100%), Positives = 687/687 (100%)

Sequence:

ATGCATATCCAATCGTTGGGGGCTACTGCCTCCTCGCTGAATCAGGAGCCTGTCGAAACCCCGTCGCAGGCA
GCGCATAAGTCCGCCAGCTTGCGTCAGGAACCTTCAGGGCAAGGTCTCGGGGTTGCCCTAAAGAGCACGCCG
GGAATACTTTCCGGGAAGTTGCCGGAAAGCGTTAGCGACGTGCGTTTCAGCAGTCCCCAAGGGCAAGGGGAG
TCCCGTACTCTGACTGACTCGGCAGGGCCGCGGCAGATCACTCTGCGCCAGTTTGAGAACGGAGTCACCGAG
CTACAGCTCAGTCGGCCACCATTGACCAGTCTGGTCCTAAGCGGCGGTGGTGCCAAAGGTGCGGCATACCCG
GGAGCAATGCTGGCGCTAGAAGAGAAAGGCATGCTCGATGGCATCCGCAGCATGTCCGGTTCGTCCGCTGGC
GGCATCACCGCCGCCCTTTTGGCCTCAGGTATGAGCCCGGCGGCGTTCAAGACCCTTTCCGACAAGATGGAT
CTTATTTCGCTGCTCGACAGCTCGAACAAGAAGCTGAAGCTGTTCCAACACATTAGCAGCGAGATCGGCGCA
TCGCTGAAAAAGGGCTTGGGCAACAAGATCGGCGGCTTCTCTGAGTTGCTGCTCAATGTACTCCCACGCATA
GATTCGCGGGCTGAGCCCCTAGAACGCCTATTGCGCGACGAGACACGCAAGGCCGTGCTCGGACAGATCGCT
ACGCATCCAGAGGTTGCACGCCAGCCGACCGTTGCCGCCATCGCCAGCAGATTGCAGTCCGGCTCCGGAGTC
ACCTTTGGCGATCTAGATCGGTTGAGTGCTTACATTCCCCAGATTAAGACGCTGAACATCACAGGTACGGCC
ATGTTCGAGGGGCGTCCGCAATTAGTGGTGTTCAATGCCAGCCACACACCGGATCTGGAGGTCGCCCAGGCG
GCACATATCTCCGGTTCCTTCCCAGGAGTGTTCCAGAAGGTCAGCTTGAGTGATCAGCCGTACCAGGCCGGC
GTAGAGTGGACAGAATTCCAGGATGGCGGGGTGATGATTAACGTGCCGGTCCCTGAGATGATCGACAAGAAT
TTTGACAGCGGGCCACTGCGGCGCAACGACAACCTGATCCTTGAGTTCGAGGGCGAAGCTGGGGAGGTAGCG
CCCGACCGAGGTACTAGGGGCGGCGCGCTCAAGGGCTGGGTCGTCGGGGTGCCTGCCCTGCAGGCGCGCGAA
ATGCTGCAGCTCGAGGGCCTGGAGGAATTGCGCGAGCAAACCGTTGTGGTGCCGTTGAAGAGCGAGCGCGGT
GATTTCAGTGGCATGCTCGGTGGCACCTTGAACTTCACCATGCCGGACGAGATCAAGGCGCATCTTCAGGAG
CGCCTCCAGGAGCGAGTCGGTGAACATCTGGAGAAACGTCTTCAGGCTTCAGAGCGTCATACCTTCGCTTCT
CTCGACGAGGCGCTGCTGGCACTTGATGACAGTATGCTCACCAGTGTTGCTCAACAGAACCCGGAGATCACA
GACGGGGCGGTGGCTTTTCGCCAGAAGGCGCGGGATGCGTTCACCGAGCTGACTGTCGCTATCGTTAGCGCC
AATGGCTTGGCGGGTAGGCTCAAGTTGGACGAGGCTATGCGCTCCGCTCTTCAGCGACTCGATGCGCTGGCA
GATACTCCGGAACGCCTAGCATGGTTGGCAGCTGAGTTGAACCATGCTGATAACGTTGATCATCAGCAGTTA
CTCGATGCCATGCGCGGGCAGACGGTGCAGTCGCCGGTGCTCGCCGCTGCGTTAGCAGAGGCGCAGCGCCGC
AAAGTGGCGGTTATTGCCGAGAACATTCGTAAGGAAGTTATCTTCCCCTCTCTGTATCGCCCTGGCCAGCCG
GATTCCAACGTAGCTCTGTTACGTCGGGCGGAGGAGCAGCTACGGCATGCCACCAGTCCGGCGGAAATCAAT
CAAGCGCTGAACGATATCGTCGACAACTACTCGGCACGAGGCTTCCTGCGTTTCGGCAAACCCTTGAGTTCG
ACTACCGTTGAGATGGCTAAGGCTTGGCGGAATAAGGAGTTCACATGA

Translation:

MHIQSLGATASSLNQEPVETPSQAAHKSASLRQEPSGQGLGVALKSTPGILSGKLPESVSDVRFSSPQGQGE
SRTLTDSAGPRQITLRQFENGVTELQLSRPPLTSLVLSGGGAKGAAYPGAMLALEEKGMLDGIRSMSGSSAG
GITAALLASGMSPAAFKTLSDKMDLISLLDSSNKKLKLFQHISSEIGASLKKGLGNKIGGFSELLLNVLPRI
DSRAEPLERLLRDETRKAVLGQIATHPEVARQPTVAAIASRLQSGSGVTFGDLDRLSAYIPQIKTLNITGTA
MFEGRPQLVVFNASHTPDLEVAQAAHISGSFPGVFQKVSLSDQPYQAGVEWTEFQDGGVMINVPVPEMIDKN
FDSGPLRRNDNLILEFEGEAGEVAPDRGTRGGALKGWVVGVPALQAREMLQLEGLEELREQTVVVPLKSERG
DFSGMLGGTLNFTMPDEIKAHLQERLQERVGEHLEKRLQASERHTFASLDEALLALDDSMLTSVAQQNPEIT
DGAVAFRQKARDAFTELTVAIVSANGLAGRLKLDEAMRSALQRLDALADTPERLAWLAAELNHADNVDHQQL
LDAMRGQTVQSPVLAAALAEAQRRKVAVIAENIRKEVIFPSLYRPGQPDSNVALLRRAEEQLRHATSPAEIN
QALNDIVDNYSARGFLRFGKPLSSTTVEMAKAWRNKEFT*

AnnotationID:11 GeneID:339

AnnotatorUID: danlee

Modification Date/Time: 2004-05-12 15:03:02

Gene Name: exoU

Confidence Code: 1

GeneProduct: ExoU

Cell Localization: (9) Secreted

Synonyms: pepA

Cell Localization Confidence Code: 5

MolecularFunction: Cytotoxin, pathogenicity related

Functional Category: (23) Secreted Factors (toxins, enzymes, alginate)

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs:

Secondary Functional Category(ies):

EC Number:

Status: ACTIVE

Pathway:

Homology:

Structural Features:

pfam01734, Patatin, Patatin-like phospholipase. Score: 87.3, 6e-18
COG1752, RssA, Predicted esterase of the alpha-beta hydrolase superfamily. Score: 61.6, 3e-10

Genomic Context:

Comment:

ReferenceID:681

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:17823

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:32078

Author/Investigator(s): Finck-Barbancon V, Goranson J, Zhu L, Sawa T, Wiener-Kronish JP, Fleiszig SM, Wu C, Mende-Mueller L, Frank DW.

Title: ExoU expression by Pseudomonas aeruginosa correlates with acute cytotoxicity and epithelial injury.

PubMed: 9302017

MedLine:

Source:

Mol Microbiol. 1997 Aug;25(3):547-57.

Reference Type: Journal

Data:

URL:

Homologs By Global Alignment

Gene ID:339

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

27198 gb|AAP82959.1| (AY273870) type III effector protein [Pseudomonas aeruginosa] 687 99.99 99.99 0 3425.0

27199 gb|AAC38269.1| (AF027291) PepA [Pseudomonas aeruginosa] 687 99.99 99.99 0 3425.0

27200 gb|AAC16023.1| (U97065) ExoU [Pseudomonas aeruginosa] 687 99.99 99.99 0 3425.0

GC ORFID: `43341`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_RS14`	Glimmer Score:
Start: `370887`	Stop: `372950`
Length: `2064`
Start Codon: `ATG`	Truncated Start:
Stop Codon: `TGA`	Truncated Stop:
Homolog: `t_RS14 ExoU (pepA) 687 Homolog: ExoU / Cytotoxin; pathogenicity-related / P. aeruginosa / PA103 (AAC38269) translation of RS14`	Homolog Bit Score `1323.0`
Other Homologs: `t_RS14 (ORF: GLIMMER)`
GC ORF Sequence
ATGCATATCCAATCGTTGGGGGCTACTGCCTCCTCGCTGAATCAGGAGCCTGTCGAAACCCCGTCGCAGGCA GCGCATAAGTCCGCCAGCTTGCGTCAGGAACCTTCAGGGCAAGGTCTCGGGGTTGCCCTAAAGAGCACGCCG GGAATACTTTCCGGGAAGTTGCCGGAAAGCGTTAGCGACGTGCGTTTCAGCAGTCCCCAAGGGCAAGGGGAG TCCCGTACTCTGACTGACTCGGCAGGGCCGCGGCAGATCACTCTGCGCCAGTTTGAGAACGGAGTCACCGAG CTACAGCTCAGTCGGCCACCATTGACCAGTCTGGTCCTAAGCGGCGGTGGTGCCAAAGGTGCGGCATACCCG GGAGCAATGCTGGCGCTAGAAGAGAAAGGCATGCTCGATGGCATCCGCAGCATGTCCGGTTCGTCCGCTGGC GGCATCACCGCCGCCCTTTTGGCCTCAGGTATGAGCCCGGCGGCGTTCAAGACCCTTTCCGACAAGATGGAT CTTATTTCGCTGCTCGACAGCTCGAACAAGAAGCTGAAGCTGTTCCAACACATTAGCAGCGAGATCGGCGCA TCGCTGAAAAAGGGCTTGGGCAACAAGATCGGCGGCTTCTCTGAGTTGCTGCTCAATGTACTCCCACGCATA GATTCGCGGGCTGAGCCCCTAGAACGCCTATTGCGCGACGAGACACGCAAGGCCGTGCTCGGACAGATCGCT ACGCATCCAGAGGTTGCACGCCAGCCGACCGTTGCCGCCATCGCCAGCAGATTGCAGTCCGGCTCCGGAGTC ACCTTTGGCGATCTAGATCGGTTGAGTGCTTACATTCCCCAGATTAAGACGCTGAACATCACAGGTACGGCC ATGTTCGAGGGGCGTCCGCAATTAGTGGTGTTCAATGCCAGCCACACACCGGATCTGGAGGTCGCCCAGGCG GCACATATCTCCGGTTCCTTCCCAGGAGTGTTCCAGAAGGTCAGCTTGAGTGATCAGCCGTACCAGGCCGGC GTAGAGTGGACAGAATTCCAGGATGGCGGGGTGATGATTAACGTGCCGGTCCCTGAGATGATCGACAAGAAT TTTGACAGCGGGCCACTGCGGCGCAACGACAACCTGATCCTTGAGTTCGAGGGCGAAGCTGGGGAGGTAGCG CCCGACCGAGGTACTAGGGGCGGCGCGCTCAAGGGCTGGGTCGTCGGGGTGCCTGCCCTGCAGGCGCGCGAA ATGCTGCAGCTCGAGGGCCTGGAGGAATTGCGCGAGCAAACCGTTGTGGTGCCGTTGAAGAGCGAGCGCGGT GATTTCAGTGGCATGCTCGGTGGCACCTTGAACTTCACCATGCCGGACGAGATCAAGGCGCATCTTCAGGAG CGCCTCCAGGAGCGAGTCGGTGAACATCTGGAGAAACGTCTTCAGGCTTCAGAGCGTCATACCTTCGCTTCT CTCGACGAGGCGCTGCTGGCACTTGATGACAGTATGCTCACCAGTGTTGCTCAACAGAACCCGGAGATCACA GACGGGGCGGTGGCTTTTCGCCAGAAGGCGCGGGATGCGTTCACCGAGCTGACTGTCGCTATCGTTAGCGCC AATGGCTTGGCGGGTAGGCTCAAGTTGGACGAGGCTATGCGCTCCGCTCTTCAGCGACTCGATGCGCTGGCA GATACTCCGGAACGCCTAGCATGGTTGGCAGCTGAGTTGAACCATGCTGATAACGTTGATCATCAGCAGTTA CTCGATGCCATGCGCGGGCAGACGGTGCAGTCGCCGGTGCTCGCCGCTGCGTTAGCAGAGGCGCAGCGCCGC AAAGTGGCGGTTATTGCCGAGAACATTCGTAAGGAAGTTATCTTCCCCTCTCTGTATCGCCCTGGCCAGCCG GATTCCAACGTAGCTCTGTTACGTCGGGCGGAGGAGCAGCTACGGCATGCCACCAGTCCGGCGGAAATCAAT CAAGCGCTGAACGATATCGTCGACAACTACTCGGCACGAGGCTTCCTGCGTTTCGGCAAACCCTTGAGTTCG ACTACCGTTGAGATGGCTAAGGCTTGGCGGAATAAGGAGTTCACATGA