ViewGene
Locus Name:
GeneID:
Location:

GeneID:3387
TrivialName: PA14_23220
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23220
PAO1 Ortholog Locus:PA3171
Sequence Length:699
Protein Length:232
Start: 2011846
Stop: 2011148
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06559.1|AE004741_4 (AE004741) 3-demethylubiquinone-9 3-methyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 230/232 (99%), Positives = 232/232 (100%)
Sequence:
ATGAGCAACGTCGACCACGCCGAGATCGCCAAATTCGAGGCACTGGCCCATCGCTGGTGGGACCGCGAGAGC
GAGTTCAAGCCGCTCCACGACATCAACCCGCTGCGCGTCAACTGGATCGACGAGCGCGCCGGCCTGGCCGGC
AAGAAGGTGCTCGACATCGGTTGCGGCGGCGGCAtCCTcaGCGAAGCcatggCCCAGCGCGGCGCCAACGTC
AccgGcAtCGacatgggCGaaGCGccgctCGcggtggcGCgcctgcACCAgCTGGAATCCGGCGTCGCCGTC
GACTACCGGCAGATCACCGCCGAGCAGATGGCCGagGAAATGCCCGGGCAATTCGACGTGGTCACCTGCCTG
GAGATGCTCGAACACGTGCCGGACCCGGCCTCGGTGATCCGCGCCTGCCACCGCCTGGTCAAGCCGGGCGGA
CAGGTTTTCCTCTCGACCATCAACCGCAATCCCAAGGCCTACCTGTTTGCGGTGATCGGCGCGGAGTACATC
CTCCAGTTGCTGCCGCGCGGGACCCACGATTTCCGCAAGTTCATCCGTCCGTCGGAACTCGGCGCCTGGTCG
CGCGAGGCCGGTCTCGAGGTCAAGGACATCATCGGCCTGACCTACAACCCGCTGACCAAGCACTACAAGCTG
GCCAACGACGTCGACGTCAACTACATGGTCCAGACCCAGCGGGAGGCGTGA
Translation:
MSNVDHAEIAKFEALAHRWWDRESEFKPLHDINPLRVNWIDERAGLAGKKVLDIGCGGGILSEAMAQRGANV
TGIDMGEAPLAVARLHQLESGVAVDYRQITAEQMAEEMPGQFDVVTCLEMLEHVPDPASVIRACHRLVKPGG
QVFLSTINRNPKAYLFAVIGAEYILQLLPRGTHDFRKFIRPSELGAWSREAGLEVKDIIGLTYNPLTKHYKL
ANDVDVNYMVQTQREA*
AnnotationID:3395GeneID:3387
AnnotatorUID: diggins
Modification Date/Time: 2005-04-13 13:01:09
Gene Name:ubiG
Confidence Code:2
GeneProduct:3-demethylubiquinone-9 3-methyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:2-octaprenyl-6-hydroxy phenol methylase
Functional Category Confidence Code:5
COGs:COG2227
Secondary Functional Category(ies):energy metabolism
EC Number:2.1.1.64
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55262.1| (AE016862) 3-demethylubiquinone-9 3-methyltransferase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 239

 Score =  437 bits (1123), Expect = e-122
 Identities = 210/232 (90%), Positives = 223/232 (96%)

>emb|CAE33967.1| (BX640447) 3-demethylubiquinone-9 3-methyltransferase [Bordetella
           bronchiseptica]
          Length = 257

 Score =  317 bits (811), Expect = 5e-86
 Identities = 149/229 (65%), Positives = 184/229 (80%), Gaps = 1/229 (0%)

>emb|CAC90053.1| (AJ414147) 3-demethylubiquinone-9 3-methyltransferase [Yersinia
           pestis CO92]
          Length = 242

 Score =  312 bits (799), Expect = 1e-84
 Identities = 150/231 (64%), Positives = 186/231 (80%), Gaps = 1/231 (0%)








 
Structural Features:
COG2227, UbiG, 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase 
[Coenzyme metabolism]
CD-Length = 243 residues,  96.7% aligned
Score =  339 bits (870), Expect = 2e-94
Identities = 150/236 (63%), Positives = 182/236 (77%), Gaps = 5/236 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:6777




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23919




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32371




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3387_cdd.html


    		




 
URL:

















    ReferenceID:33953




  
Author/Investigator(s):
        Wu G, Williams HD, Zamanian M, Gibson F, Poole RK.





  
Title:
        Isolation and characterization of Escherichia coli mutants affected in aerobic respiration: the cloning and nucleotide sequence of ubiG. Identification of an S-adenosylmethionine-binding motif in protein, RNA, and small-molecule methyltransferases.




  
PubMed:
        1479344


MedLine:
        




    
Source:
J Gen Microbiol. 1992 Oct;138 ( Pt 10):2101-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1479344

















    ReferenceID:33954




  
Author/Investigator(s):
        Gibert I, Llagostera M, Barbe J.





  
Title:
        Regulation of ubiG gene expression in Escherichia coli.




  
PubMed:
        2830238


MedLine:
        




    
Source:
J Bacteriol. 1988 Mar;170(3):1346-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2830238









           
Homologs By Global Alignment 

           

       



     
Gene ID:3387
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5419
                           PA3171_tr
                           translation of PA3171
                           232
                           99.13
                           99.99
                           0
                           1206.0
     

     
     
151318
                           gb|AAG06559.1|AE004741_4
                           (AE004741) 3-demethylubiquinone-9 3-methyltransferase [Pseudomonas aeruginosa PAO1]
                           232
                           99.13
                           99.99
                           0
                           1206.0
     

     
     
151319
                           gb|AAO55262.1|
                           (AE016862) 3-demethylubiquinone-9 3-methyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           239
                           87.86
                           93.30
                           7
                           1123.0
     

     
     
151320
                           gb|AAN67385.1|AE016364_10
                           (AE016780) 3-demethylubiquinone-9 3-methyltransferase [Pseudomonas putida KT2440]
                           232
                           87.06
                           94.39
                           0
                           1087.0
     

     
     
151321
                           gb|AAQ58706.1|
                           (AE016913) 3-demethylubiquinone-9 3-O-methyltransferase [Chromobacterium violaceum ATCC 12472]
                           232
                           62.93
                           79.74
                           0
                           813.0
     

     






      

          
GC ORFID: 46389How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3171Glimmer Score: 

          
Start: 2942085Stop: 2942783

          
Length: 699


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3171 translation of PA3171Homolog Bit Score 469.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7689 (ORF: BLASTX 431.0), t_PA3171 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAACGTCGACCACGCCGAGATCGCCAAATTCGAGGCACTGGCCCATCGCTGGTGGGACCGCGAGAGC 
GAGTTCAAGCCGCTCCACGACATCAACCCGCTGCGCGTCAACTGGATCGACGAGCGCGCCGGCCTGGCCGGC 
AAGAAGGTGCTCGACATCGGTTGCGGCGGCGGCAtCCTcaGCGAAGCcatggCCCAGCGCGGCGCCAACGTC 
AccgGcAtCGacatgggCGaaGCGccgctCGcggtggcGCgcctgcACCAgCTGGAATCCGGCGTCGCCGTC 
GACTACCGGCAGATCACCGCCGAGCAGATGGCCGagGAAATGCCCGGGCAATTCGACGTGGTCACCTGCCTG 
GAGATGCTCGAACACGTGCCGGACCCGGCCTCGGTGATCCGCGCCTGCCACCGCCTGGTCAAGCCGGGCGGA 
CAGGTTTTCCTCTCGACCATCAACCGCAATCCCAAGGCCTACCTGTTTGCGGTGATCGGCGCGGAGTACATC 
CTCCAGTTGCTGCCGCGCGGGACCCACGATTTCCGCAAGTTCATCCGTCCGTCGGAACTCGGCGCCTGGTCG 
CGCGAGGCCGGTCTCGAGGTCAAGGACATCATCGGCCTGACCTACAACCCGCTGACCAAGCACTACAAGCTG 
GCCAACGACGTCGACGTCAACTACATGGTCCAGACCCAGCGGGAGGCGTGA