ViewGene
Locus Name:
GeneID:
Location:

GeneID:3373
TrivialName: PA14_37000
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_37000
PAO1 Ortholog Locus:PA2132
Sequence Length:714
Protein Length:237
Start: 3295567
Stop: 3294854
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05520.1|AE004640_9 (AE004640) chaperone CupA5 [Pseudomonas aeruginosa PAO1]
Identities = 237/237 (100%), Positives = 237/237 (100%)
Sequence:
GTGTCCAGTAGGCGCGCGCTCGCGCTGCTGGCCATGCTTTGCCTCGCGCCGACGAGCGAGGCCGGAGTGACC
GCGGAGCGCACCCGCCTGGTGTTCGTGGAGGGCCAGCGCGAGGCCTCGTTGCTGCTGGTCAACCAGAATCCC
TACCCGGTGCTGGTGCAGGCGTGGGTCGATGACGGCGCCCTCGACGGCGAACCGGATACCGCGCTGGCTCCG
TTCCTGCCGCTGCCGCCCGTATTCCGCCTGGAGCCGGGACGCCAGCGCAGCCTGCGCCTGCTGGCTACCGGC
CAGGCCCAGGCACGGGATCGCGAATCGCTGTACTGGCTGAACATCTACGAGATCCCCCCGCAAGCCACCGCC
GAGGCCGCTGGCCGGCCCCGACTCACGGTCACCCTGCGAACCCAGATGAAGGTGCTGTACCGCCCGCACGGC
CTGCATCCGCACGCTGAGGACGCGGCCGCCGAACTGGGCTTCGCGCTGCGCGACGAGACCCTGCGGGTGGAC
AACCCGACGCCGTACTTCGTGTCCCTGGCGGGGCTGGCGCTGGAGATAGGGGAGACGCGCCTGGATCTGCCC
GGAACGCTGCTGGCTCCGCGCTCGCAACGACGCCTCGGCCTGCCGCGGCGGGTGGACGGCGGGACGCGCGGC
GAGGTGGCGTTCGACTGGATCGACGACAACGGCGTCAGCCGCCAGGAAAGGAAAGCTCTACAGTGA
Translation:
MSSRRALALLAMLCLAPTSEAGVTAERTRLVFVEGQREASLLLVNQNPYPVLVQAWVDDGALDGEPDTALAP
FLPLPPVFRLEPGRQRSLRLLATGQAQARDRESLYWLNIYEIPPQATAEAAGRPRLTVTLRTQMKVLYRPHG
LHPHAEDAAAELGFALRDETLRVDNPTPYFVSLAGLALEIGETRLDLPGTLLAPRSQRRLGLPRRVDGGTRG
EVAFDWIDDNGVSRQERKALQ*
AnnotationID:3381GeneID:3373
AnnotatorUID: diggins
Modification Date/Time: 2005-03-03 10:10:46
Gene Name:cupA5
Confidence Code:1
GeneProduct:chaperone CupA5
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:pilus assembly chaperone
Functional Category Confidence Code:5
COGs:COG3121,pfam00345
Secondary Functional Category(ies):motility and attachment
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM83938.1|AE013635_3 (AE013635) putative pilus chaperone, PapD family [Yersinia pestis
           KIM]
          Length = 272

 Score =  175 bits (444), Expect = 2e-43
 Identities = 105/226 (46%), Positives = 133/226 (58%), Gaps = 10/226 (4%)

>emb|CAC93348.1| (AJ414159) putative chaperone protein [Yersinia pestis CO92]
          Length = 248

 Score =  175 bits (444), Expect = 2e-43
 Identities = 105/226 (46%), Positives = 133/226 (58%), Gaps = 10/226 (4%)

>gb|AAM85950.1|AE013842_5 (AE013842) putative pilin chaperone [Yersinia pestis KIM]
          Length = 246

 Score =  149 bits (375), Expect = 2e-35
 Identities = 74/184 (40%), Positives = 117/184 (63%), Gaps = 5/184 (2%)








 
Structural Features:
COG3121, FimC, P pilus assembly protein, chaperone PapD 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 235 residues,  97.4% aligned
Score =  209 bits (533), Expect = 3e-55
Identities = 95/236 (40%), Positives = 134/236 (56%), Gaps = 7/236 (2%)

pfam00345, Pili_assembly_N, Gram-negative pili assembly chaperone, N-terminal domain.
CD-Length = 122 residues,  99.2% aligned
Score =  120 bits (303), Expect = 1e-28
Identities = 50/125 (40%), Positives = 79/125 (63%), Gaps = 5/125 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14807




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23891




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32592




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3373_cdd.html


    		




 
URL:

















    ReferenceID:34422




  
Author/Investigator(s):
        Vallet I, Olson JW, Lory S, Lazdunski A, Filloux A.





  
Title:
        The chaperone/usher pathways of Pseudomonas aeruginosa: identification of fimbrial gene clusters (cup) and their involvement in biofilm formation.




  
PubMed:
        11381121


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6911-6. Epub 2001 May 29.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=11381121









           
Homologs By Global Alignment 

           

       



     
Gene ID:3373
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5403
                           PA2132_tr
                           translation of PA2132
                           237
                           99.99
                           99.99
                           0
                           1211.0
     

     
     
151131
                           gb|AAG05520.1|AE004640_9
                           (AE004640) chaperone CupA5 [Pseudomonas aeruginosa PAO1]
                           237
                           99.57
                           99.99
                           0
                           1208.0
     

     
     
151132
                           gb|AAP84167.1|
                           (AY273869) putative pili assembly chaperone [Pseudomonas aeruginosa]
                           241
                           59.75
                           70.12
                           7
                           648.0
     

     
     
151134
                           emb|CAC93348.1|
                           (AJ414159) putative chaperone protein [Yersinia pestis CO92]
                           258
                           44.18
                           55.42
                           31
                           449.0
     

     
     
151133
                           gb|AAM83938.1|AE013635_3
                           (AE013635) putative pilus chaperone, PapD family [Yersinia pestis KIM]
                           282
                           40.42
                           50.70
                           55
                           449.0
     

     






      

          
GC ORFID: 46375How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2132Glimmer Score: 

          
Start: 1658347Stop: 1659060

          
Length: 714


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2132 translation of PA2132Homolog Bit Score 471.0

          
Other Homologs:  t_RL040 (ORF: BLASTX 251.0), t_PA2132 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGTCCAGTAGGCGCGCGCTCGCGCTGCTGGCCATGCTTTGCCTCGCGCCGACGAGCGAGGCCGGAGTGACC 
GCGGAGCGCACCCGCCTGGTGTTCGTGGAGGGCCAGCGCGAGGCCTCGTTGCTGCTGGTCAACCAGAATCCC 
TACCCGGTGCTGGTGCAGGCGTGGGTCGATGACGGCGCCCTCGACGGCGAACCGGATACCGCGCTGGCTCCG 
TTCCTGCCGCTGCCGCCCGTATTCCGCCTGGAGCCGGGACGCCAGCGCAGCCTGCGCCTGCTGGCTACCGGC 
CAGGCCCAGGCACGGGATCGCGAATCGCTGTACTGGCTGAACATCTACGAGATCCCCCCGCAAGCCACCGCC 
GAGGCCGCTGGCCGGCCCCGACTCACGGTCACCCTGCGAACCCAGATGAAGGTGCTGTACCGCCCGCACGGC 
CTGCATCCGCACGCTGAGGACGCGGCCGCCGAACTGGGCTTCGCGCTGCGCGACGAGACCCTGCGGGTGGAC 
AACCCGACGCCGTACTTCGTGTCCCTGGCGGGGCTGGCGCTGGAGATAGGGGAGACGCGCCTGGATCTGCCC 
GGAACGCTGCTGGCTCCGCGCTCGCAACGACGCCTCGGCCTGCCGCGGCGGGTGGACGGCGGGACGCGCGGC 
GAGGTGGCGTTCGACTGGATCGACGACAACGGCGTCAGCCGCCAGGAAAGGAAAGCTCTACAGTGA