ViewGene
Locus Name:
GeneID:
Location:

GeneID:3349
TrivialName: PA14_42270
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_42270
PAO1 Ortholog Locus:PA1723
Sequence Length:747
Protein Length:248
Start: 3770665
Stop: 3769919
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05112.1|AE004598_11 (AE004598) type III export protein PscJ [Pseudomonas aeruginosa PAO1]
Identities = 248/248 (100%), Positives = 248/248 (100%)
Sequence:
ATGAGGCGAACGGTGAAAGGTCTGTCGCGCATGGCGCTCCTGGCGCTGGTCCTGGCGTTGGGCGGGTGCAAG
GTCGAACTCTATACCGGGATCAGCCAGAAGGAAGGCAACGAGATGCTGGCGCTGTTGCGTTCGGAGGGCGTT
TCCGCGGACAAGCAGGCGGACAAGGATGGCACCGTGCGCCTGCTGGTGGAGGAGTCGGATATCGCCGAGGCG
GTGGAGGTGCTCAAGCGCAAGGGCTATCCGCGGGAGAACTTCTCCACGCTGAAGGATGTGTTTCCCAAGGAC
GGGCTGATTTCCTCGCCCATCGAAGAGCGCGCGCGGCTCAACTACGCCAAGGCCCAGGAGATTTCCCATACC
CTTTCCGAGATCGATGGCGTGCTGGTGGCGCGGGTCCATGTGGTGCTGCCCGAGGAGCGCGACGGACTGGGC
AGGAAGTCCTCGCCGGCATCGGCCTCGGTGTTCATCAAGCACGCCGCCGACGTGCAACTGGATGCCTACGTG
CCGCAGATCAAGCAACTGGTGAACAACGGCATCGAAGGACTCAGCTACGACCGCATCAGCGTGGTGCTGGTG
CCGTCGGCCGGGGTCCGCCAGGTCCCGCTGGCGCCACGCTTCGAGAGTGTCTTCTCGATCCAGGTGGCGGAG
CACTCGCGCGGACGCCTGCTCGGTCTGTTCGGCCTGCTGCTGGCGTTGCTGCTGGCGAGCAACCTGGCGCAG
TTCTTCTGGCACCGGCAACGGGGCTGA
Translation:
MRRTVKGLSRMALLALVLALGGCKVELYTGISQKEGNEMLALLRSEGVSADKQADKDGTVRLLVEESDIAEA
VEVLKRKGYPRENFSTLKDVFPKDGLISSPIEERARLNYAKAQEISHTLSEIDGVLVARVHVVLPEERDGLG
RKSSPASASVFIKHAADVQLDAYVPQIKQLVNNGIEGLSYDRISVVLVPSAGVRQVPLAPRFESVFSIQVAE
HSRGRLLGLFGLLLALLLASNLAQFFWHRQRG*
AnnotationID:3357GeneID:3349
AnnotatorUID: diggins
Modification Date/Time: 2006-01-22 11:11:04
Gene Name:pscJ
Confidence Code:1
GeneProduct:pscJ type III export protein
Cell Localization:()
Synonyms:PA1723
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(23) Secreted Factors (toxins, enzymes, alginate)
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam01514, COG4669
Secondary Functional Category(ies):Intracellular trafficking and secretion
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO18027.1| (AY144116) LscJ [Photorhabdus luminescens]
          Length = 245

 Score =  378 bits (971), Expect = e-104
 Identities = 189/237 (79%), Positives = 214/237 (90%)

>gb|AAN37545.2| (AY150843) YscJ [Yersinia enterocolitica]
          Length = 244

 Score =  342 bits (877), Expect = 1e-93
 Identities = 172/243 (70%), Positives = 206/243 (84%)

>gb|AAC69775.1| (AF074612) type III secretion protein [Yersinia pestis KIM]
          Length = 244

 Score =  340 bits (872), Expect = 4e-93
 Identities = 171/243 (70%), Positives = 205/243 (84%)






 
Structural Features:
pfam01514, YscJ_FliF, Secretory protein of YscJ/FliF family. 
CD-Length = 203 residues,  93.6% aligned
Score =  220 bits (562), Expect = 1e-58
Identities = 106/192 (55%), Positives = 131/192 (68%), Gaps = 4/192 (2%)

COG4669, EscJ, Type III secretory pathway, lipoprotein EscJ
[Intracellular trafficking and secretion]
Length = 246
Score =  302 bits (776), Expect = 3e-83
Identities = 134/240 (55%), Positives = 171/240 (71%), Gaps = 2/240 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6701




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23843




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38261




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3349_cdd.html


    		




 
URL:

















    ReferenceID:38262




  
Author/Investigator(s):
        Yahr TL, Goranson J, Frank DW.





  
Title:
        Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway.




  
PubMed:
        8971719


MedLine:
        




    
Source:
Mol Microbiol. 1996 Dec;22(5):991-1003.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=97126825&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:3349
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5368
                           PA1723_tr
                           translation of PA1723
                           248
                           99.99
                           99.99
                           0
                           1222.0
     

     
     
150236
                           gb|AAG05112.1|AE004598_11
                           (AE004598) type III export protein PscJ [Pseudomonas aeruginosa PAO1]
                           248
                           99.99
                           99.99
                           0
                           1222.0
     

     
     
150237
                           gb|AAC44781.1|
                           (U56077) PscJ [Pseudomonas aeruginosa]
                           248
                           99.59
                           99.59
                           0
                           1214.0
     

     
     
150238
                           gb|AAO18027.1|
                           (AY144116) LscJ [Photorhabdus luminescens]
                           249
                           76.30
                           86.74
                           5
                           964.0
     

     
     
150239
                           emb|CAE16157.1|
                           (BX571871) Type III secretion component protein SctJ [Photorhabdus luminescens subsp. laumondii TTO1]
                           249
                           75.90
                           86.34
                           5
                           955.0
     

     






      

          
GC ORFID: 46351How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1723Glimmer Score: 

          
Start: 1183244Stop: 1183990

          
Length: 747


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1723 translation of PA1723Homolog Bit Score 475.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4485 (ORF: BLASTX 153.0), t_PA1723 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGGCGAACGGTGAAAGGTCTGTCGCGCATGGCGCTCCTGGCGCTGGTCCTGGCGTTGGGCGGGTGCAAG 
GTCGAACTCTATACCGGGATCAGCCAGAAGGAAGGCAACGAGATGCTGGCGCTGTTGCGTTCGGAGGGCGTT 
TCCGCGGACAAGCAGGCGGACAAGGATGGCACCGTGCGCCTGCTGGTGGAGGAGTCGGATATCGCCGAGGCG 
GTGGAGGTGCTCAAGCGCAAGGGCTATCCGCGGGAGAACTTCTCCACGCTGAAGGATGTGTTTCCCAAGGAC 
GGGCTGATTTCCTCGCCCATCGAAGAGCGCGCGCGGCTCAACTACGCCAAGGCCCAGGAGATTTCCCATACC 
CTTTCCGAGATCGATGGCGTGCTGGTGGCGCGGGTCCATGTGGTGCTGCCCGAGGAGCGCGACGGACTGGGC 
AGGAAGTCCTCGCCGGCATCGGCCTCGGTGTTCATCAAGCACGCCGCCGACGTGCAACTGGATGCCTACGTG 
CCGCAGATCAAGCAACTGGTGAACAACGGCATCGAAGGACTCAGCTACGACCGCATCAGCGTGGTGCTGGTG 
CCGTCGGCCGGGGTCCGCCAGGTCCCGCTGGCGCCACGCTTCGAGAGTGTCTTCTCGATCCAGGTGGCGGAG 
CACTCGCGCGGACGCCTGCTCGGTCTGTTCGGCCTGCTGCTGGCGTTGCTGCTGGCGAGCAACCTGGCGCAG 
TTCTTCTGGCACCGGCAACGGGGCTGA