ViewGene
Locus Name:
GeneID:
Location:

GeneID:3346
TrivialName: PA14_11270
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_11270
PAO1 Ortholog Locus:PA4067
Sequence Length:699
Protein Length:232
Start: 981869
Stop: 981171
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07454.1|AE004822_14 (AE004822) Outer membrane protein OprG precursor [Pseudomonas aeruginosa PAO1]
Identities = 232/232 (100%), Positives = 232/232 (100%)
Sequence:
ATGCGTAAGTCCTGGCTTACCGCTTCCCTGCTGGCCCTCACCGTCGCCTCCCCGTTCGCCGCTGCGGATATT
CAAGGACACAAGGCCGGTGACTTCATCATCCGTGGCGGCTTCGCCACCGTCGATCCCGACGACAGCAGTTCC
GACATCAAGCTGGACGGCGCCAAGCAGCGCGGTACCAAGGCGACCGTCGACAGCGACACCCAGCTCGGCCTG
ACCTTCACCTACATGTTCGCCGACAAGTGGGGCGTGGAACTGGTCGCCGCCACTCCGTTCAACCACCAGGTC
GACGTCAAGGGCCTCGGCCCGGGCCTGGACGGCAAGCTGGCCGACATCAAGCAACTGCCGCCGACCCTGCTG
CTCCAGTACTACCCCATGGGCGGTACCAACAGCGCCTTCCAGCCCTACGGCGGCCTGGGCGTGAACTACACC
ACCTTCTTCGACGAAGACCTCGCCAGCAACCGCAAGGCGCAGGGTTTCAGCAGCATGAAGCTGCAGGACTCC
TGGGGCCTGGCCGGCGAACTGGGCTTCGACTACATGCTCAACGAGCACGCGCTGTTCAACATGGCCGTCTGG
TACATGGACATCGACACCAAGGCCAGCATCAACGGCCCGAGCGCCCTGGGCGTGAACAAGACCAAGGTCGAC
GTGGACGTCGATCCGTGGGTCTACATGATCGGTTTCGGCTACAAGTTCTGA
Translation:
MRKSWLTASLLALTVASPFAAADIQGHKAGDFIIRGGFATVDPDDSSSDIKLDGAKQRGTKATVDSDTQLGL
TFTYMFADKWGVELVAATPFNHQVDVKGLGPGLDGKLADIKQLPPTLLLQYYPMGGTNSAFQPYGGLGVNYT
TFFDEDLASNRKAQGFSSMKLQDSWGLAGELGFDYMLNEHALFNMAVWYMDIDTKASINGPSALGVNKTKVD
VDVDPWVYMIGFGYKF*
AnnotationID:3354GeneID:3346
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 06:06:12
Gene Name:oprG
Confidence Code:1
GeneProduct:outer membrane protein OprG precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(15) Membrane proteins
Alternate Gene Product Name:outer membrane protein OmpW
Functional Category Confidence Code:5
COGs:COG3047,pfam03922
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66132.1|AE016241_9 (AE016775) outer membrane protein OprG [Pseudomonas putida KT2440]
          Length = 227

 Score =  316 bits (809), Expect = 8e-86
 Identities = 158/232 (68%), Positives = 185/232 (79%), Gaps = 5/232 (2%)

>gb|AAO54221.1| (AE016858) outer membrane protein OmpW [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 228

 Score =  304 bits (779), Expect = 2e-82
 Identities = 145/232 (62%), Positives = 183/232 (78%), Gaps = 4/232 (1%)






 
Structural Features:
COG3047, OmpW, Outer membrane protein W 
[Cell envelope biogenesis, outer membrane]
CD-Length = 213 residues, 100.0% aligned
Score =  233 bits (595), Expect = 2e-62
Identities = 112/227 (49%), Positives = 136/227 (59%), Gaps = 14/227 (6%)

pfam03922, OmpW, OmpW family.
CD-Length = 189 residues, 100.0% aligned
Score =  222 bits (566), Expect = 3e-59
Identities = 100/205 (48%), Positives = 122/205 (59%), Gaps = 16/205 (7%)







   
Genomic Context:






   
Comment:


















    ReferenceID:6695




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23837




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36818




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3346_cdd.html


    		




 
URL:

















    ReferenceID:36934




  
Author/Investigator(s):
        Gensberg K, Smith AW, Brinkman FS, Hancock RE.





  
Title:
        Identification of oprG, a gene encoding a major outer membrane protein of Pseudomonas aeruginosa.




  
PubMed:
        10350397


MedLine:
        




    
Source:
J Antimicrob Chemother. 1999 Apr;43(4):607-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10350397









           
Homologs By Global Alignment 

           

       



     
Gene ID:3346
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5365
                           PA4067_tr
                           translation of PA4067
                           232
                           99.99
                           99.99
                           0
                           1225.0
     

     
     
150129
                           gb|AAG07454.1|AE004822_14
                           (AE004822) Outer membrane protein OprG precursor [Pseudomonas aeruginosa PAO1]
                           232
                           99.99
                           99.99
                           0
                           1225.0
     

     
     
150130
                           gb|AAN66132.1|AE016241_9
                           (AE016775) outer membrane protein OprG [Pseudomonas putida KT2440]
                           232
                           68.10
                           79.74
                           5
                           814.0
     

     
     
150131
                           gb|AAO54221.1|
                           (AE016858) outer membrane protein OmpW [Pseudomonas syringae pv. tomato str. DC3000]
                           232
                           62.50
                           78.87
                           4
                           784.0
     

     
     
150148
                           gb|AAL20650.1|
                           (AE008777) outer membrane protein W [Salmonella typhimurium LT2]
                           237
                           44.30
                           58.22
                           30
                           470.0
     

     






      

          
GC ORFID: 46348How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4067Glimmer Score: 

          
Start: 3972068Stop: 3972766

          
Length: 699


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4067 translation of PA4067Homolog Bit Score 476.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1335 (ORF: BLASTX 310.0), t_PA4067 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGTAAGTCCTGGCTTACCGCTTCCCTGCTGGCCCTCACCGTCGCCTCCCCGTTCGCCGCTGCGGATATT 
CAAGGACACAAGGCCGGTGACTTCATCATCCGTGGCGGCTTCGCCACCGTCGATCCCGACGACAGCAGTTCC 
GACATCAAGCTGGACGGCGCCAAGCAGCGCGGTACCAAGGCGACCGTCGACAGCGACACCCAGCTCGGCCTG 
ACCTTCACCTACATGTTCGCCGACAAGTGGGGCGTGGAACTGGTCGCCGCCACTCCGTTCAACCACCAGGTC 
GACGTCAAGGGCCTCGGCCCGGGCCTGGACGGCAAGCTGGCCGACATCAAGCAACTGCCGCCGACCCTGCTG 
CTCCAGTACTACCCCATGGGCGGTACCAACAGCGCCTTCCAGCCCTACGGCGGCCTGGGCGTGAACTACACC 
ACCTTCTTCGACGAAGACCTCGCCAGCAACCGCAAGGCGCAGGGTTTCAGCAGCATGAAGCTGCAGGACTCC 
TGGGGCCTGGCCGGCGAACTGGGCTTCGACTACATGCTCAACGAGCACGCGCTGTTCAACATGGCCGTCTGG 
TACATGGACATCGACACCAAGGCCAGCATCAACGGCCCGAGCGCCCTGGGCGTGAACAAGACCAAGGTCGAC 
GTGGACGTCGATCCGTGGGTCTACATGATCGGTTTCGGCTACAAGTTCTGA