ViewGene
Locus Name:
GeneID:
Location:

GeneID:3333
TrivialName: PA14_11020
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_11020
PAO1 Ortholog Locus:PA4089
Sequence Length:762
Protein Length:253
Start: 954126
Stop: 953365
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07476.1|AE004825_5 (AE004825) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 252/253 (99%), Positives = 253/253 (100%)
Sequence:
GTGAGGCGCTTGGAGAACCGCGTCGCACTGGTCACCGGCGCCGGCCAGGGCATCGGCCGGGCCATCGCCGAA
TGCTTCGCCGCCGAAGGCGCCAGGGTTCTGCTGGCGACGCGTACCGCCAGCCGCGGCCAGGCCGTCCTGGAG
GCGATCCGCCTGGGCGGAGGCACGGCCGAACTGCTGGCCGTCGACCTGAGCGAGCACGACGCCGCGAGACGA
GCGGTGAGCGCGACCCTGGAGCGCTTCGGCCAGTTGGACATCCTCCTGCACAACGCCGCTGCCTTTCCCCAG
TGCGCCCTCGCGGAACTGGACGAAGGCACCCTGGAACGGACCCTGGCGGTGAACCTGAAAAGCTGCTTCAGG
CTGACCCAGGCCGCCCTTCCCGCCCTGCGCCGCAGCGTGGCCGGGCGCGTCCTGGTGACCTCCTCGGTGACC
GGTCCGCGCACGGCGATCCCTGGGCTCTCGCACTATGCGGCGAGCAAGGCCGGGGTCAACGGTTTCATCCGC
GCCGCCGCGCTGGAGCTGGCCGGCGACGGCATCACCGTCAACGGCGTCGAGCCCGGCCTGGTCGCGACTCCG
GCGCTGGGCAGCCTCGGCGACGCCGCCGCGCTGGCCGCGCACATCCCCCTCGGGCGTGTCGGCCAGCCGCTG
GACATCGCCCACGCCATGCTCTTCCTCGCCTCCGACGAGGCGAGCTACATCACCGGGCAGACCCTGGTTGTG
GATGGCGGCGCGTTGCTGCCGGAAAACGGCGGGCTGGCCTGA
Translation:
MRRLENRVALVTGAGQGIGRAIAECFAAEGARVLLATRTASRGQAVLEAIRLGGGTAELLAVDLSEHDAARR
AVSATLERFGQLDILLHNAAAFPQCALAELDEGTLERTLAVNLKSCFRLTQAALPALRRSVAGRVLVTSSVT
GPRTAIPGLSHYAASKAGVNGFIRAAALELAGDGITVNGVEPGLVATPALGSLGDAAALAAHIPLGRVGQPL
DIAHAMLFLASDEASYITGQTLVVDGGALLPENGGLA*
AnnotationID:3341GeneID:3333
AnnotatorUID: diggins
Modification Date/Time: 2005-06-07 13:01:22
Gene Name:
Confidence Code:3
GeneProduct:putative short-chain dehydrogenase
Cell Localization:()
Synonyms:fabG
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:putative 3-oxoacyl-(acyl-carrier protein) reductase
Functional Category Confidence Code:5
COGs:pfam00106,COG1028,COG4221
Secondary Functional Category(ies):fatty acid and phospholipid metabolism
EC Number:1.1.1.100
Status:ACTIVE
Pathway:
Fatty acid biosynthesis pathway; first reduction step.
Homology:
>dbj|BAB54421.1| (AP003014) 3-ketoacyl-acyl carrier protein reductase [Mesorhizobium
           loti]
          Length = 256

 Score =  218 bits (556), Expect = 2e-56
 Identities = 119/249 (47%), Positives = 163/249 (65%), Gaps = 8/249 (3%)

>gb|AAL45939.1| (AE008947) 3-oxoacyl-(acyl-carrier protein) reductase
           [Agrobacterium tumefaciens str. C58 (U. Washington)]
          Length = 257

>dbj|BAB52956.1| (AP003010) 3-ketoacyl-CoA reductase [Mesorhizobium loti]
          Length = 254

 Score =  189 bits (479), Expect = 2e-47
 Identities = 105/248 (42%), Positives = 148/248 (59%), Gaps = 5/248 (2%)
Structural Features:
pfam00106, adh_short, short chain dehydrogenase.
CD-Length = 247 residues,  99.6% aligned
Score =  179 bits (456), Expect = 3e-46
Identities = 110/247 (44%), Positives = 144/247 (58%), Gaps = 13/247 (5%)

COG1028, FabG, Dehydrogenases with different specificities 
(related to short-chain alcohol dehydrogenases) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 251 residues,  98.8% aligned
Score =  154 bits (390), Expect = 8e-39
Identities = 118/252 (46%), Positives = 149/252 (59%), Gaps = 15/252 (5%)

COG4221, COG4221, Short-chain alcohol dehydrogenase of unknown specificity
CD-Length = 246 residues,  92.3% aligned
Score =  136 bits (345), Expect = 2e-33
Identities = 85/230 (36%), Positives = 118/230 (51%), Gaps = 6/230 (2%)
Genomic Context:






   
Comment:


















    ReferenceID:14799




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23811




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36835




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3333_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3333
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5347
                           PA4089_tr
                           translation of PA4089
                           253
                           99.60
                           99.99
                           0
                           1232.0
     

     
     
150011
                           gb|AAG07476.1|AE004825_5
                           (AE004825) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           253
                           99.20
                           99.99
                           0
                           1229.0
     

     
     
150012
                           dbj|BAB54421.1|
                           (AP003014) 3-ketoacyl-acyl carrier protein reductase [Mesorhizobium loti]
                           261
                           46.74
                           62.45
                           13
                           559.0
     

     
     
150013
                           gb|AAL45939.1|
                           (AE008947) 3-oxoacyl-(acyl-carrier protein) reductase [Agrobacterium tumefaciens str. C58 (U. Washington)]
                           260
                           42.30
                           58.46
                           10
                           515.0
     

     
     
150014
                           gb|AAK90624.1|
                           (AE007895) AGR_pAT_355p [Agrobacterium tumefaciens str. C58 (Cereon)]
                           260
                           42.30
                           58.46
                           10
                           515.0
     

     






      

          
GC ORFID: 46335How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4089Glimmer Score: 

          
Start: 3999811Stop: 4000572

          
Length: 762


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4089 translation of PA4089Homolog Bit Score 479.0

          
Other Homologs:  t_PA4089 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAGGCGCTTGGAGAACCGCGTCGCACTGGTCACCGGCGCCGGCCAGGGCATCGGCCGGGCCATCGCCGAA 
TGCTTCGCCGCCGAAGGCGCCAGGGTTCTGCTGGCGACGCGTACCGCCAGCCGCGGCCAGGCCGTCCTGGAG 
GCGATCCGCCTGGGCGGAGGCACGGCCGAACTGCTGGCCGTCGACCTGAGCGAGCACGACGCCGCGAGACGA 
GCGGTGAGCGCGACCCTGGAGCGCTTCGGCCAGTTGGACATCCTCCTGCACAACGCCGCTGCCTTTCCCCAG 
TGCGCCCTCGCGGAACTGGACGAAGGCACCCTGGAACGGACCCTGGCGGTGAACCTGAAAAGCTGCTTCAGG 
CTGACCCAGGCCGCCCTTCCCGCCCTGCGCCGCAGCGTGGCCGGGCGCGTCCTGGTGACCTCCTCGGTGACC 
GGTCCGCGCACGGCGATCCCTGGGCTCTCGCACTATGCGGCGAGCAAGGCCGGGGTCAACGGTTTCATCCGC 
GCCGCCGCGCTGGAGCTGGCCGGCGACGGCATCACCGTCAACGGCGTCGAGCCCGGCCTGGTCGCGACTCCG 
GCGCTGGGCAGCCTCGGCGACGCCGCCGCGCTGGCCGCGCACATCCCCCTCGGGCGTGTCGGCCAGCCGCTG 
GACATCGCCCACGCCATGCTCTTCCTCGCCTCCGACGAGGCGAGCTACATCACCGGGCAGACCCTGGTTGTG 
GATGGCGGCGCGTTGCTGCCGGAAAACGGCGGGCTGGCCTGA