ViewGene
Locus Name:
GeneID:
Location:

GeneID:3332
TrivialName: PA14_16050
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_16050
PAO1 Ortholog Locus:PA3737
Sequence Length:729
Protein Length:242
Start: 1367515
Stop: 1368243
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07124.1|AE004793_1 (AE004793) thiol:disulfide interchange protein DsbC [Pseudomonas aeruginosa PAO1]
Identities = 242/242 (100%), Positives = 242/242 (100%)
Sequence:
ATGCGCGTGACCCGTTTTCTGGCTGCTGCCGCCCTCGGCCTGATGAGTACCCTGGCCCTGGCAGACAATGCC
GATCAGAACATCCGCAAGACCCTGCAGGCCCTGCAACCGGACCTGCCCATCGACTCCATCGCCAGCAGCCCG
CTGCAGGGGCTCTACCAGGTGCAGCTGAAGGGCGGCCGGGTGCTCTACGCCAGCGCCGACGGCCAGTTCGTC
ATGCAGGGCTACCTCTACCAGGTGAAGGACGGCAAGCCGGTCAACCTGACCGAGAAGGCCGAGAGCCAGGCG
ATCGCCAAGGCGATCAACGGCGTGCCCGCCAGCGAGATGGTGGTGTATCCGGCCAAGGGCCAGGCCAAGGCG
CACATCACGGTGTTCACCGATACCACCTGCCCGTACTGCCAGAAGCTGCATGCCGAGGTGCCGGACCTGACC
GAGCAGGGGATCGAGGTGCGCTACATGGCGTTCCCGCGGCAGGGGCCGCAGTCGGCGGGCGACAAGCAACTG
CAGGCGGTCTGGTGTGCGAAGGAGCCGACCAAGGCGATGGACGCCATGATGAACGGCAAGGAGATCAAGTCT
TCCGAGTGCAAGAACCCGGTCGACAAGCAGTTCCAGATGGGCCAGATGGTCGGGGTGCAGGGAACGCCGGCG
ATCGTCCTCGCCAACGGCCAGTTGCTCCCCGGCTACCAGCCGGCCAAGCAGTTGGCCAAGCTGGCGCTGGAG
GCCAAATAG
Translation:
MRVTRFLAAAALGLMSTLALADNADQNIRKTLQALQPDLPIDSIASSPLQGLYQVQLKGGRVLYASADGQFV
MQGYLYQVKDGKPVNLTEKAESQAIAKAINGVPASEMVVYPAKGQAKAHITVFTDTTCPYCQKLHAEVPDLT
EQGIEVRYMAFPRQGPQSAGDKQLQAVWCAKEPTKAMDAMMNGKEIKSSECKNPVDKQFQMGQMVGVQGTPA
IVLANGQLLPGYQPAKQLAKLALEAK*
AnnotationID:3340GeneID:3332
AnnotatorUID: diggins
Modification Date/Time: 2005-04-22 08:08:49
Gene Name:dsbC
Confidence Code:2
GeneProduct:thiol:disulfide interchange protein DsbC
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:protein disulfide isomerase II
Functional Category Confidence Code:5
COGs:COG1651
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67091.1|AE016338_1 (AE016779) thiol:disulfide interchange protein DsbC [Pseudomonas
           putida KT2440]
          Length = 260

 Score =  348 bits (894), Expect = 1e-95
 Identities = 169/245 (68%), Positives = 205/245 (83%), Gaps = 6/245 (2%)

>gb|AAM86825.1|AE013928_10 (AE013928) protein disulfide isomerase II [Yersinia pestis KIM]
          Length = 238

 Score =  178 bits (451), Expect = 3e-44
 Identities = 97/225 (43%), Positives = 142/225 (63%), Gaps = 10/225 (4%)








 
Structural Features:
COG1651, DsbG, Protein-disulfide isomerase 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 244 residues,  98.4% aligned
Score =  104 bits (259), Expect = 2e-23
Identities = 60/250 (24%), Positives = 102/250 (40%), Gaps = 18/250 (7%)








   
Genomic Context:






   
Comment:


















    ReferenceID:6667




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23809




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31820




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3332_cdd.html


    		




 
URL:

















    ReferenceID:34257




  
Author/Investigator(s):
        Missiakas D, Georgopoulos C, Raina S.





  
Title:
        The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation.




  
PubMed:
        8168498


MedLine:
        




    
Source:
EMBO J. 1994 Apr 15;13(8):2013-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8168498

















    ReferenceID:34258




  
Author/Investigator(s):
        Zapun A, Missiakas D, Raina S, Creighton TE.





  
Title:
        Structural and functional characterization of DsbC, a protein involved in disulfide bond formation in Escherichia coli.




  
PubMed:
        7536035


MedLine:
        




    
Source:
Biochemistry. 1995 Apr 18;34(15):5075-89.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7536035

















    ReferenceID:34259




  
Author/Investigator(s):
        Shevchik VE, Condemine G, Robert-Baudouy J.





  
Title:
        Characterization of DsbC, a periplasmic protein of Erwinia chrysanthemi and Escherichia coli with disulfide isomerase activity.




  
PubMed:
        8168497


MedLine:
        




    
Source:
EMBO J. 1994 Apr 15;13(8):2007-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8168497









           
Homologs By Global Alignment 

           

       



     
Gene ID:3332
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5346
                           PA3737_tr
                           translation of PA3737
                           242
                           99.99
                           99.99
                           0
                           1234.0
     

     
     
149990
                           gb|AAG07124.1|AE004793_1
                           (AE004793) thiol:disulfide interchange protein DsbC [Pseudomonas aeruginosa PAO1]
                           242
                           99.99
                           99.99
                           0
                           1234.0
     

     
     
149991
                           gb|AAC16483.1|
                           (AF057031) putative thiol:disulfide interchange protein precursor; DsbC [Pseudomonas aeruginosa]
                           242
                           99.99
                           99.99
                           0
                           1234.0
     

     
     
149993
                           gb|AAO55000.1|
                           (AE016861) thiol:disulfide interchange protein DsbC [Pseudomonas syringae pv. tomato str. DC3000]
                           246
                           66.26
                           82.52
                           4
                           848.0
     

     
     
149992
                           gb|AAN67091.1|AE016338_1
                           (AE016779) thiol:disulfide interchange protein DsbC [Pseudomonas putida KT2440]
                           261
                           65.51
                           79.31
                           20
                           897.0
     

     






      

          
GC ORFID: 46334How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3737Glimmer Score: 

          
Start: 3586422Stop: 3585694

          
Length: 729


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA3737 translation of PA3737Homolog Bit Score 479.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4567 (ORF: BLASTX 331.0), t_PA3737 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCGTGACCCGTTTTCTGGCTGCTGCCGCCCTCGGCCTGATGAGTACCCTGGCCCTGGCAGACAATGCC 
GATCAGAACATCCGCAAGACCCTGCAGGCCCTGCAACCGGACCTGCCCATCGACTCCATCGCCAGCAGCCCG 
CTGCAGGGGCTCTACCAGGTGCAGCTGAAGGGCGGCCGGGTGCTCTACGCCAGCGCCGACGGCCAGTTCGTC 
ATGCAGGGCTACCTCTACCAGGTGAAGGACGGCAAGCCGGTCAACCTGACCGAGAAGGCCGAGAGCCAGGCG 
ATCGCCAAGGCGATCAACGGCGTGCCCGCCAGCGAGATGGTGGTGTATCCGGCCAAGGGCCAGGCCAAGGCG 
CACATCACGGTGTTCACCGATACCACCTGCCCGTACTGCCAGAAGCTGCATGCCGAGGTGCCGGACCTGACC 
GAGCAGGGGATCGAGGTGCGCTACATGGCGTTCCCGCGGCAGGGGCCGCAGTCGGCGGGCGACAAGCAACTG 
CAGGCGGTCTGGTGTGCGAAGGAGCCGACCAAGGCGATGGACGCCATGATGAACGGCAAGGAGATCAAGTCT 
TCCGAGTGCAAGAACCCGGTCGACAAGCAGTTCCAGATGGGCCAGATGGTCGGGGTGCAGGGAACGCCGGCG 
ATCGTCCTCGCCAACGGCCAGTTGCTCCCCGGCTACCAGCCGGCCAAGCAGTTGGCCAAGCTGGCGCTGGAG 
GCCAAATAG