ViewGene
Locus Name:
GeneID:
Location:

GeneID:333
TrivialName: PA14_07530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_07530
PAO1 Ortholog Locus:PA0577
Sequence Length:1995
Protein Length:664
Start: 651118
Stop: 649124
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03966.1|AE004494_2 (AE004494) DNA primase [Pseudomonas aeruginosa PAO1]
Identities = 663/664 (99%), Positives = 663/664 (99%)
Sequence:
ATGGCCGGCCTGATACCGCAAAGCTTCATCGATGACCTGCTGAACCGCACCGACATCGTCGAGGTGGTGAGT
TCGCGCATCCAGCTGAAGAAAACCGGCAAGAACTACAGCGCCTGCTGCCCGTTCCACAAGGAAAAGACTCCC
TCGTTCACCGTCAGCCCGGACAAGCAGTTCTACTACTGCTTCGGCTGCGGCGCCGGCGGCAACGCGCTCGGC
TTCGTCATGGACCACGACCAGTTGGAGTTCCCCCAGGCGGTCGAGGAACTGGCCAAGCGCGCCGGCATGGAC
GTGCCGCGCGAGGAACGCGGCGGACGCGGGCACACGCCGCGCCAGCCGACCGACTCGCCGCTCTACCCGCTG
CTCAGCGCCGCCGCCGAGTTCTACAAGCAGGCGCTGAAAAGCCACCCGGCGCGCAAGGCCGCGGTGAACTAC
CTGAAGGGCCGCGGCCTGACCGGCGAGATCGCCCGCGACTTCGGCCTCGGCTTCGCCCCGCCTGGCTGGGAC
AACCTGCTCAAGCACCTGGGTGGCGACAACCTGCAACTCAAGGCCATGCTCGACGCCGGCCTGCTGGTGGAG
AACAGCGACACCGGCAAGCGCTACGACCGCTTCCGCGATCGCGTGATGTTCCCGATCCGCGACAGCCGCGGG
CGGATCATCGCCTTCGGCGGCCGGGTGCTCGGCGACGACAAGCCGAAGTACCTGAACTCCCCGGAGACTCCG
GTCTTCCACAAGGGCCAGGAACTCTACGGACTCTACGAAGCCCGGCAGAAGAATCGCGACCTCGACGAGATC
ATGGTGGTCGAGGGCTACATGGATGTCATCGCCCTGGCCCAGCAAGGCATCCGCAACGCGGTGGCGACCCTC
GGCACGGCGACCAGCGAGGAACACATCAAGCGCCTGTTCCGCCTGGTACCGAGCATCCTGTTCTGTTTCGAC
GGCGACCAGGCCGGCCGCAAGGCGGCCTGGCGCGCCCTGGAGTCGGTGCTGCCGAACCTGCAGGACGGCAAG
CGCGTGCGCTTCCTGTTCCTCCCCGAGGGGGAGGACCCGGACAGCCTGGTACGCGCCGAGGGCGAGGATGCC
TTCCGCGCACGCATCACCCAGCAGGCCCAGCCGCTGGCGGAGTACTTCTTCCAGCAACTGATGCTGGAGGCC
GACCCCGCCACCCTGGAAGGCAAGGCCCACCTGGCGACCCTCGCCGCGCCGCTGCTGGAGAAGATTCCCGGG
AACAACCTGCGCCTGCTGATGCGGCAACGTCTGTCGGAAATCACCGGCCTGTCCGGCGAAAACATCGGACAA
CTGGCGCACCACAGCCCGCCACCGTCGTCCATGGACCACGGAGCGAGCGGCGTCTTGGACGGCGACGACTAT
TTCGCCGCCAGCGCCTACTACGAGAACGAGCCCTCCCATGCGCCGTTCGACGCTGCGCCAGGCTATGGCGAG
GCGCAACCGCGCAAGAGCTGGAACAAGGACAAGAAGCCCTGGGACGGCAAGAAGTGGGATGGCAAGAAGAAG
TGGGACAAAGGCGGCCGCGGCGACTTCAAGGCTCCGCAACGAACGCCGGTCAGCGTGGAGTCCACCACCCTC
AACGCCCTGCGCACCTTGCTGCATCATCCGCAGTTGGCCTTGAAGGTCGACGATGCGGGCACCCTCGCCAGG
GAGCAGGATACCTACGCCCAGTTGCTGGTGTCCCTGCTGGAAGCCCTGCAGAAGAACCCGCGGCAGAGCTCG
ATGCAACTCATCGCGCGCTGGCACGGCACGCCCCAGGGCCGCCTGCTGCAGGCCCTGGGCGAGAAGGAGTGG
CTGATCGTCCAGGAGAACCTTGAAAAGCAGTTCTTCGACACCATTACTAAACTCTCCGAGAGCCAGCGTTTC
GGCGAGCGGGAGGAGCGTTTACGCAGCGTCATGCAGAAGAGCTACAGTGAACTGACCGATGAAGAGAAAGCC
TTGCTCAGAGAGCACTACAGTGTTGCCGCTTCATCGCCTTCCCAGAGCTGA
Translation:
MAGLIPQSFIDDLLNRTDIVEVVSSRIQLKKTGKNYSACCPFHKEKTPSFTVSPDKQFYYCFGCGAGGNALG
FVMDHDQLEFPQAVEELAKRAGMDVPREERGGRGHTPRQPTDSPLYPLLSAAAEFYKQALKSHPARKAAVNY
LKGRGLTGEIARDFGLGFAPPGWDNLLKHLGGDNLQLKAMLDAGLLVENSDTGKRYDRFRDRVMFPIRDSRG
RIIAFGGRVLGDDKPKYLNSPETPVFHKGQELYGLYEARQKNRDLDEIMVVEGYMDVIALAQQGIRNAVATL
GTATSEEHIKRLFRLVPSILFCFDGDQAGRKAAWRALESVLPNLQDGKRVRFLFLPEGEDPDSLVRAEGEDA
FRARITQQAQPLAEYFFQQLMLEADPATLEGKAHLATLAAPLLEKIPGNNLRLLMRQRLSEITGLSGENIGQ
LAHHSPPPSSMDHGASGVLDGDDYFAASAYYENEPSHAPFDAAPGYGEAQPRKSWNKDKKPWDGKKWDGKKK
WDKGGRGDFKAPQRTPVSVESTTLNALRTLLHHPQLALKVDDAGTLAREQDTYAQLLVSLLEALQKNPRQSS
MQLIARWHGTPQGRLLQALGEKEWLIVQENLEKQFFDTITKLSESQRFGEREERLRSVMQKSYSELTDEEKA
LLREHYSVAASSPSQS*
AnnotationID:343GeneID:333
AnnotatorUID: diggins
Modification Date/Time: 2005-03-29 08:08:13
Gene Name:dnaG
Confidence Code:2
GeneProduct:putative DNA primase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam01807
Secondary Functional Category(ies):
EC Number:2.7.7.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66019.1|AE016231_2 (AE016775) DNA primase [Pseudomonas putida KT2440]
          Length = 660

 Score =  943 bits (2437), Expect = 0.0
 Identities = 487/669 (72%), Positives = 543/669 (81%), Gaps = 16/669 (2%)

>gb|AAO54080.1| (AE016857) DNA primase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 652

 Score =  912 bits (2358), Expect = 0.0
 Identities = 471/672 (70%), Positives = 544/672 (80%), Gaps = 30/672 (4%)

>gb|AAM87084.1|AE013956_9 (AE013956) DNA primase [Yersinia pestis KIM]
          Length = 582

 Score =  498 bits (1283), Expect = e-140
 Identities = 245/425 (57%), Positives = 324/425 (76%), Gaps = 4/425 (0%)






 
Structural Features:
pfam01807, zf-CHC2, CHC2 zinc finger. 
CD-Length = 98 residues,  99.0% aligned
Score =  176 bits (447), Expect = 8e-45
Identities = 57/97 (58%), Positives = 78/97 (80%)






   
Genomic Context:






   
Comment:


















    ReferenceID:669




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17811




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33869




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:333_cdd.html


    		




 
URL:

















    ReferenceID:35277




  
Author/Investigator(s):
        Szafranski P, Smith CL, Cantor CR.





  
Title:
        Cloning and analysis of the dnaG gene encoding Pseudomonas putida DNA primase.




  
PubMed:
        9224947


MedLine:
        




    
Source:
Biochim Biophys Acta. 1997 Jun 26;1352(3):243-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9224947

















    ReferenceID:35278




  
Author/Investigator(s):
        Versalovic J, Lupski JR.





  
Title:
        The Haemophilus influenzae dnaG sequence and conserved bacterial primase motifs.




  
PubMed:
        8294018


MedLine:
        




    
Source:
Gene. 1993 Dec 22;136(1-2):281-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8294018

















    ReferenceID:35279




  
Author/Investigator(s):
        Almond N, Yajnik V, Svec P, Godson GN.





  
Title:
        An Escherichia coli cis-acting antiterminator sequence: the dnaG nut site.




  
PubMed:
        2664448


MedLine:
        




    
Source:
Mol Gen Genet. 1989 Apr;216(2-3):195-203.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2664448

















    ReferenceID:35280




  
Author/Investigator(s):
        Erickson BD, Burton ZF, Watanabe KK, Burgess RR.





  
Title:
        Nucleotide sequence of the rpsU-dnaG-rpoD operon from Salmonella typhimurium and a comparison of this sequence with the homologous operon of Escherichia coli.




  
PubMed:
        3005129


MedLine:
        




    
Source:
Gene. 1985;40(1):67-78.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3005129

















    ReferenceID:35281




  
Author/Investigator(s):
        Smiley BL, Lupski JR, Svec PS, McMacken R, Godson GN.





  
Title:
        Sequences of the Escherichia coli dnaG primase gene and regulation of its expression.




  
PubMed:
        6750604


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4550-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6750604









           
Homologs By Global Alignment 

           

       



     
Gene ID:333
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
552
                           PA0577_tr
                           translation of PA0577
                           664
                           99.84
                           99.84
                           0
                           3463.0
     

     
     
27041
                           gb|AAG03966.1|AE004494_2
                           (AE004494) DNA primase [Pseudomonas aeruginosa PAO1]
                           664
                           99.84
                           99.84
                           0
                           3463.0
     

     
     
27042
                           gb|AAN66019.1|AE016231_2
                           (AE016775) DNA primase [Pseudomonas putida KT2440]
                           679
                           72.45
                           81.29
                           34
                           2458.0
     

     
     
27043
                           gb|AAC45508.1|
                           (U85774) DNA primase [Pseudomonas putida]
                           679
                           72.45
                           81.29
                           34
                           2458.0
     

     
     
27044
                           gb|AAC38072.1|
                           (AF014397) DNA primase [Pseudomonas putida]
                           679
                           72.45
                           81.29
                           34
                           2458.0
     

     






      

          
GC ORFID: 43335How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0577Glimmer Score: 

          
Start: 6452461Stop: 6450467

          
Length: 1995


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0577 translation of PA0577Homolog Bit Score 1338.0

          
Other Homologs:  t_PA0577 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCGGCCTGATACCGCAAAGCTTCATCGATGACCTGCTGAACCGCACCGACATCGTCGAGGTGGTGAGT 
TCGCGCATCCAGCTGAAGAAAACCGGCAAGAACTACAGCGCCTGCTGCCCGTTCCACAAGGAAAAGACTCCC 
TCGTTCACCGTCAGCCCGGACAAGCAGTTCTACTACTGCTTCGGCTGCGGCGCCGGCGGCAACGCGCTCGGC 
TTCGTCATGGACCACGACCAGTTGGAGTTCCCCCAGGCGGTCGAGGAACTGGCCAAGCGCGCCGGCATGGAC 
GTGCCGCGCGAGGAACGCGGCGGACGCGGGCACACGCCGCGCCAGCCGACCGACTCGCCGCTCTACCCGCTG 
CTCAGCGCCGCCGCCGAGTTCTACAAGCAGGCGCTGAAAAGCCACCCGGCGCGCAAGGCCGCGGTGAACTAC 
CTGAAGGGCCGCGGCCTGACCGGCGAGATCGCCCGCGACTTCGGCCTCGGCTTCGCCCCGCCTGGCTGGGAC 
AACCTGCTCAAGCACCTGGGTGGCGACAACCTGCAACTCAAGGCCATGCTCGACGCCGGCCTGCTGGTGGAG 
AACAGCGACACCGGCAAGCGCTACGACCGCTTCCGCGATCGCGTGATGTTCCCGATCCGCGACAGCCGCGGG 
CGGATCATCGCCTTCGGCGGCCGGGTGCTCGGCGACGACAAGCCGAAGTACCTGAACTCCCCGGAGACTCCG 
GTCTTCCACAAGGGCCAGGAACTCTACGGACTCTACGAAGCCCGGCAGAAGAATCGCGACCTCGACGAGATC 
ATGGTGGTCGAGGGCTACATGGATGTCATCGCCCTGGCCCAGCAAGGCATCCGCAACGCGGTGGCGACCCTC 
GGCACGGCGACCAGCGAGGAACACATCAAGCGCCTGTTCCGCCTGGTACCGAGCATCCTGTTCTGTTTCGAC 
GGCGACCAGGCCGGCCGCAAGGCGGCCTGGCGCGCCCTGGAGTCGGTGCTGCCGAACCTGCAGGACGGCAAG 
CGCGTGCGCTTCCTGTTCCTCCCCGAGGGGGAGGACCCGGACAGCCTGGTACGCGCCGAGGGCGAGGATGCC 
TTCCGCGCACGCATCACCCAGCAGGCCCAGCCGCTGGCGGAGTACTTCTTCCAGCAACTGATGCTGGAGGCC 
GACCCCGCCACCCTGGAAGGCAAGGCCCACCTGGCGACCCTCGCCGCGCCGCTGCTGGAGAAGATTCCCGGG 
AACAACCTGCGCCTGCTGATGCGGCAACGTCTGTCGGAAATCACCGGCCTGTCCGGCGAAAACATCGGACAA 
CTGGCGCACCACAGCCCGCCACCGTCGTCCATGGACCACGGAGCGAGCGGCGTCTTGGACGGCGACGACTAT 
TTCGCCGCCAGCGCCTACTACGAGAACGAGCCCTCCCATGCGCCGTTCGACGCTGCGCCAGGCTATGGCGAG 
GCGCAACCGCGCAAGAGCTGGAACAAGGACAAGAAGCCCTGGGACGGCAAGAAGTGGGATGGCAAGAAGAAG 
TGGGACAAAGGCGGCCGCGGCGACTTCAAGGCTCCGCAACGAACGCCGGTCAGCGTGGAGTCCACCACCCTC 
AACGCCCTGCGCACCTTGCTGCATCATCCGCAGTTGGCCTTGAAGGTCGACGATGCGGGCACCCTCGCCAGG 
GAGCAGGATACCTACGCCCAGTTGCTGGTGTCCCTGCTGGAAGCCCTGCAGAAGAACCCGCGGCAGAGCTCG 
ATGCAACTCATCGCGCGCTGGCACGGCACGCCCCAGGGCCGCCTGCTGCAGGCCCTGGGCGAGAAGGAGTGG 
CTGATCGTCCAGGAGAACCTTGAAAAGCAGTTCTTCGACACCATTACTAAACTCTCCGAGAGCCAGCGTTTC 
GGCGAGCGGGAGGAGCGTTTACGCAGCGTCATGCAGAAGAGCTACAGTGAACTGACCGATGAAGAGAAAGCC 
TTGCTCAGAGAGCACTACAGTGTTGCCGCTTCATCGCCTTCCCAGAGCTGA