ViewGene
Locus Name:
GeneID:
Location:

GeneID:3322
TrivialName: PA14_17080
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17080
PAO1 Ortholog Locus:PA3654
Sequence Length:738
Protein Length:245
Start: 1465051
Stop: 1465788
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07042.1|AE004785_6 (AE004785) uridylate kinase [Pseudomonas aeruginosa PAO1]
Identities = 245/245 (100%), Positives = 245/245 (100%)
Sequence:
ATGGCTCAGCAACTGAGCGCTCGTCAACCTCGCTATAAACGCATTCTTCTAAAGTTGAGCGGCGAAGCCCTG
ATGGGCTCGGAGGAGTTCGGCATCGATCCCAAGGTGCTGGACCGCATGGCGCTGGAAATCGGCCAGTTGGTC
GGGATCGGCGTGCAGGTCGGCCTGGTCATCGGCGGCGGCAACCTGTTCCGCGGAGCGGCCCTGTCCGCGGCC
GGCATGGACCGGGTGACCGGCGACCACATGGGGATGCTGGCCACCGTGATGAACGGCCTGGCGATGCGCGAT
GCGCTGGAGCGCTCGAACATCCCCGCGCTGGTGATGTCGGCGATCTCCATGGTCGGCGTGACCGACCACTAC
GACCGCCGCAAGGCCATGCGCCACCTCGGCGGTGGCGAGGTGGTGATCTTCTCCGCCGGTACCGGCAACCCG
TTCTTCACCACCGACTCGGCGGCTTGCCTGCGCGCCATCGAGATCGACGCCGACGTGGTCCTTAAGGCTACC
AAGGTCGATGGCGTGTACACTGCCGACCCGTTCAAGGACCCGAATGCCGAGAAGTTCGAGCGCCTGACCTAT
GATGAAGTGCTCGATCGCAAGCTCGGCGTGATGGACCTGACCGCCATCTGCCTGTGCCGTGACCAGAACATG
CCGCTGCGGGTGTTCAACATGAACAAGCCGGGCGCATTGCTGAATATTGTTGTTGGTGGTGCCGAAGGCACC
CTGATCGAGGAGGGTTGA
Translation:
MAQQLSARQPRYKRILLKLSGEALMGSEEFGIDPKVLDRMALEIGQLVGIGVQVGLVIGGGNLFRGAALSAA
GMDRVTGDHMGMLATVMNGLAMRDALERSNIPALVMSAISMVGVTDHYDRRKAMRHLGGGEVVIFSAGTGNP
FFTTDSAACLRAIEIDADVVLKATKVDGVYTADPFKDPNAEKFERLTYDEVLDRKLGVMDLTAICLCRDQNM
PLRVFNMNKPGALLNIVVGGAEGTLIEEG*
AnnotationID:3330GeneID:3322
AnnotatorUID: diggins
Modification Date/Time: 2005-04-21 05:05:10
Gene Name:pyrH
Confidence Code:2
GeneProduct:uridylate kinase
Cell Localization:()
Synonyms:smbA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0528,pfam00696
Secondary Functional Category(ies):
EC Number:2.7.4.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67214.1|AE016348_6 (AE016779) uridylate kinase [Pseudomonas putida KT2440]
          Length = 247

 Score =  460 bits (1183), Expect = e-129
 Identities = 232/245 (94%), Positives = 239/245 (97%)

>gb|AAO10264.1|AE016803_51 (AE016803) Uridylate kinase [Vibrio vulnificus CMCP6]
          Length = 241

 Score =  323 bits (828), Expect = 6e-88
 Identities = 165/234 (70%), Positives = 194/234 (82%)

>gb|AAG54473.1|AE005193_3 (AE005193) uridylate kinase [Escherichia coli O157:H7 EDL933]
          Length = 241

 Score =  317 bits (811), Expect = 5e-86
 Identities = 163/236 (69%), Positives = 189/236 (80%)






 
Structural Features:
COG0528, PyrH, Uridylate kinase [Nucleotide transport and metabolism]
CD-Length = 238 residues,  99.6% aligned
Score =  328 bits (842), Expect = 8e-91
Identities = 147/237 (62%), Positives = 180/237 (75%), Gaps = 1/237 (0%)

pfam00696, AA_kinase, Amino acid kinase family.
CD-Length = 236 residues, 100.0% aligned
Score =  131 bits (331), Expect = 1e-31
Identities = 71/241 (29%), Positives = 107/241 (44%), Gaps = 36/241 (14%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6647




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23789




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31889




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3322_cdd.html


    		




 
URL:

















    ReferenceID:34221




  
Author/Investigator(s):
        Wadskov-Hansen SL, Martinussen J, Hammer K.





  
Title:
        The pyrH gene of Lactococcus lactis subsp. cremoris encoding UMP kinase is transcribed as part of an operon including the frr1 gene encoding ribosomal recycling factor 1.




  
PubMed:
        10607910


MedLine:
        




    
Source:
Gene. 2000 Jan 4;241(1):157-66.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10607910

















    ReferenceID:34222




  
Author/Investigator(s):
        Serina L, Blondin C, Krin E, Sismeiro O, Danchin A, Sakamoto H, Gilles AM, Barzu O.





  
Title:
        Escherichia coli UMP-kinase, a member of the aspartokinase family, is a hexamer regulated by guanine nucleotides and UTP.




  
PubMed:
        7711027


MedLine:
        




    
Source:
Biochemistry. 1995 Apr 18;34(15):5066-74.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7711027

















    ReferenceID:34223




  
Author/Investigator(s):
        Ohnishi M, Janosi L, Shuda M, Matsumoto H, Hayashi T, Terawaki Y, Kaji A.





  
Title:
        Molecular cloning, sequencing, purification, and characterization of Pseudomonas aeruginosa ribosome recycling factor.




  
PubMed:
        9973356


MedLine:
        




    
Source:
J Bacteriol. 1999 Feb;181(4):1281-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9973356









           
Homologs By Global Alignment 

           

       



     
Gene ID:3322
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5324
                           PA3654_tr
                           translation of PA3654
                           245
                           99.99
                           99.99
                           0
                           1237.0
     

     
     
148963
                           gb|AAG07042.1|AE004785_6
                           (AE004785) uridylate kinase [Pseudomonas aeruginosa PAO1]
                           245
                           99.99
                           99.99
                           0
                           1237.0
     

     
     
148964
                           gb|AAN67214.1|AE016348_6
                           (AE016779) uridylate kinase [Pseudomonas putida KT2440]
                           247
                           93.92
                           96.76
                           2
                           1183.0
     

     
     
148966
                           dbj|BAA32344.1|
                           (AB010087) UMP kinase [Pseudomonas aeruginosa]
                           246
                           92.68
                           93.90
                           2
                           1115.0
     

     
     
148965
                           gb|AAO55056.1|
                           (AE016861) uridylate kinase [Pseudomonas syringae pv. tomato str. DC3000]
                           247
                           91.09
                           93.52
                           2
                           1132.0
     

     






      

          
GC ORFID: 46324How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3654Glimmer Score: 

          
Start: 3488886Stop: 3488149

          
Length: 738


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3654 translation of PA3654Homolog Bit Score 481.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4621 (ORF: BLASTX 441.0), t_PA3654 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTCAGCAACTGAGCGCTCGTCAACCTCGCTATAAACGCATTCTTCTAAAGTTGAGCGGCGAAGCCCTG 
ATGGGCTCGGAGGAGTTCGGCATCGATCCCAAGGTGCTGGACCGCATGGCGCTGGAAATCGGCCAGTTGGTC 
GGGATCGGCGTGCAGGTCGGCCTGGTCATCGGCGGCGGCAACCTGTTCCGCGGAGCGGCCCTGTCCGCGGCC 
GGCATGGACCGGGTGACCGGCGACCACATGGGGATGCTGGCCACCGTGATGAACGGCCTGGCGATGCGCGAT 
GCGCTGGAGCGCTCGAACATCCCCGCGCTGGTGATGTCGGCGATCTCCATGGTCGGCGTGACCGACCACTAC 
GACCGCCGCAAGGCCATGCGCCACCTCGGCGGTGGCGAGGTGGTGATCTTCTCCGCCGGTACCGGCAACCCG 
TTCTTCACCACCGACTCGGCGGCTTGCCTGCGCGCCATCGAGATCGACGCCGACGTGGTCCTTAAGGCTACC 
AAGGTCGATGGCGTGTACACTGCCGACCCGTTCAAGGACCCGAATGCCGAGAAGTTCGAGCGCCTGACCTAT 
GATGAAGTGCTCGATCGCAAGCTCGGCGTGATGGACCTGACCGCCATCTGCCTGTGCCGTGACCAGAACATG 
CCGCTGCGGGTGTTCAACATGAACAAGCCGGGCGCATTGCTGAATATTGTTGTTGGTGGTGCCGAAGGCACC 
CTGATCGAGGAGGGTTGA