ViewGene
Locus Name:
GeneID:
Location:

GeneID:3319
TrivialName: PA14_26220
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_26220
PAO1 Ortholog Locus:PA2925
Sequence Length:714
Protein Length:237
Start: 2289190
Stop: 2288477
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06313.1|AE004718_10 (AE004718) histidine transport system permease HisM [Pseudomonas aeruginosa PAO1]
Identities = 237/237 (100%), Positives = 237/237 (100%)
Sequence:
ATGAACGCGGTACTCGACGAATACTGGCAGGCCTACCTGTGGACCGACGGCCTGCAACTGACCGGACTGGCG
ATGACCCTGTGGCTGCTGATCCTCGCCGTGGCGCTTGGCTTCCTGCTGGCCATCCCGCTGGCGGTGGCGCGA
ACCTGCGGCAACCCGCTGCTGCGCTACCCGGTCTGGCTCTACACCTACGTGTTCCGCGGCACCCCGCTGTAC
ATCCAGTTGCTGTTCTTCTACACCGGCGTCTACAGCCTGAATGCCATACGCGCCCAGGACTTTCTCAACGCG
TTCTTCCGCGACGGCTTCAACTGCACGGTACTGGCCTTCGTCCTCAACACCTGCGCCTACATGACGGAAATC
TTCGCCGGCGCGATCAGGAACACGCCCCACGGCGAAGTGGAAGCGGCGCGCGCCTACGGCATGGGCGGCTTC
ACCCTCTACCGGCGCATCGTCCTGCCCTCGGCGCTGCGCCGGGCACTGCCGTACTTCAGCAACGAAGTGATC
CTGATGCTGCATTCCACCTCGGTGGCGTTCACCGCCACCGTGCCGGACCTGCTCAAGGTGGCCCGCGACGTC
AATTCCGCCACCTATGCGCCGTTCCTGTCCTTCGGCATCGCCGGCGCGCTCTACGCCGGCACCGCCTTCCTG
CTGATCTGGCTGTTCCGCCGCGGCGAGCTGCGCTGGCTGGCCTACCTCAGACCGCAAACCCACTGA
Translation:
MNAVLDEYWQAYLWTDGLQLTGLAMTLWLLILAVALGFLLAIPLAVARTCGNPLLRYPVWLYTYVFRGTPLY
IQLLFFYTGVYSLNAIRAQDFLNAFFRDGFNCTVLAFVLNTCAYMTEIFAGAIRNTPHGEVEAARAYGMGGF
TLYRRIVLPSALRRALPYFSNEVILMLHSTSVAFTATVPDLLKVARDVNSATYAPFLSFGIAGALYAGTAFL
LIWLFRRGELRWLAYLRPQTH*
AnnotationID:3327GeneID:3319
AnnotatorUID: diggins
Modification Date/Time: 2005-04-08 10:10:15
Gene Name:hisM
Confidence Code:2
GeneProduct:histidine ABC transporter, inner membrane permease
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG4160,COG0765
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM85178.1|AE013764_2 (AE013764) histidine ABC transporter, inner membrane permease
           [Yersinia pestis KIM]
          Length = 238

 Score =  325 bits (834), Expect = 1e-88
 Identities = 153/235 (65%), Positives = 188/235 (80%)

>gb|AAP17714.1| (AE016986) histidine transport, membrane protein M [Shigella
           flexneri 2a str. 2457T]
          Length = 238

 Score =  322 bits (826), Expect = 9e-88
 Identities = 149/236 (63%), Positives = 190/236 (80%)

>gb|AAN81303.1|AE016763_262 (AE016763) Histidine transport system permease protein hisM
           [Escherichia coli CFT073]
          Length = 238

 Score =  322 bits (826), Expect = 9e-88
 Identities = 149/236 (63%), Positives = 190/236 (80%)






 
Structural Features:
COG4160, ArtM, ABC-type arginine/histidine transport system, permease component 
[Amino acid transport and metabolism]
CD-Length = 228 residues, 100.0% aligned
Score =  282 bits (724), Expect = 2e-77
Identities = 125/228 (54%), Positives = 151/228 (66%)

COG0765, HisM, ABC-type amino acid transport system, permease component 
[Amino acid transport and metabolism]
CD-Length = 222 residues,  91.4% aligned
Score =  155 bits (394), Expect = 4e-39
Identities = 77/212 (36%), Positives = 111/212 (52%), Gaps = 9/212 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6641




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23783




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36119




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3319_cdd.html


    		




 
URL:

















    ReferenceID:36418




  
Author/Investigator(s):
        Higgins CF, Haag PD, Nikaido K, Ardeshir F, Garcia G, Ames GF.





  
Title:
        Complete nucleotide sequence and identification of membrane components of the histidine transport operon of S. typhimurium.




  
PubMed:
        7050725


MedLine:
        




    
Source:
Nature. 1982 Aug 19;298(5876):723-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7050725









           
Homologs By Global Alignment 

           

       



     
Gene ID:3319
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5319
                           PA2925_tr
                           translation of PA2925
                           237
                           99.99
                           99.99
                           0
                           1239.0
     

     
     
148866
                           gb|AAG06313.1|AE004718_10
                           (AE004718) histidine transport system permease HisM [Pseudomonas aeruginosa PAO1]
                           237
                           99.99
                           99.99
                           0
                           1239.0
     

     
     
148867
                           gb|AAQ58529.1|
                           (AE016912) histidine transport system membrane protein M [Chromobacterium violaceum ATCC 12472]
                           237
                           64.97
                           81.43
                           0
                           852.0
     

     
     
148868
                           gb|AAM85178.1|AE013764_2
                           (AE013764) histidine ABC transporter, inner membrane permease [Yersinia pestis KIM]
                           238
                           64.70
                           79.83
                           1
                           833.0
     

     
     
148869
                           emb|CAC93015.1|
                           (AJ414153) histidine transport system permease protein HisM [Yersinia pestis CO92]
                           238
                           64.70
                           79.83
                           1
                           833.0
     

     






      

          
GC ORFID: 46321How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2925Glimmer Score: 

          
Start: 2664735Stop: 2665448

          
Length: 714


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2925 translation of PA2925Homolog Bit Score 481.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6101 (ORF: BLASTX 317.0), t_PA0890 (ORF: BLASTX 209.0), psyr_15may02_Scaffold8_revised_gene7810 (ORF: BLASTX 205.0), t_PA5155 (ORF: BLASTX 158.0), psyr_15may02_Scaffold6_revised_gene6953 (ORF: BLASTX 146.0), t_PA2925 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGCGGTACTCGACGAATACTGGCAGGCCTACCTGTGGACCGACGGCCTGCAACTGACCGGACTGGCG 
ATGACCCTGTGGCTGCTGATCCTCGCCGTGGCGCTTGGCTTCCTGCTGGCCATCCCGCTGGCGGTGGCGCGA 
ACCTGCGGCAACCCGCTGCTGCGCTACCCGGTCTGGCTCTACACCTACGTGTTCCGCGGCACCCCGCTGTAC 
ATCCAGTTGCTGTTCTTCTACACCGGCGTCTACAGCCTGAATGCCATACGCGCCCAGGACTTTCTCAACGCG 
TTCTTCCGCGACGGCTTCAACTGCACGGTACTGGCCTTCGTCCTCAACACCTGCGCCTACATGACGGAAATC 
TTCGCCGGCGCGATCAGGAACACGCCCCACGGCGAAGTGGAAGCGGCGCGCGCCTACGGCATGGGCGGCTTC 
ACCCTCTACCGGCGCATCGTCCTGCCCTCGGCGCTGCGCCGGGCACTGCCGTACTTCAGCAACGAAGTGATC 
CTGATGCTGCATTCCACCTCGGTGGCGTTCACCGCCACCGTGCCGGACCTGCTCAAGGTGGCCCGCGACGTC 
AATTCCGCCACCTATGCGCCGTTCCTGTCCTTCGGCATCGCCGGCGCGCTCTACGCCGGCACCGCCTTCCTG 
CTGATCTGGCTGTTCCGCCGCGGCGAGCTGCGCTGGCTGGCCTACCTCAGACCGCAAACCCACTGA