ViewGene
Locus Name:
GeneID:
Location:

GeneID:3308
TrivialName: PA14_06530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_06530
PAO1 Ortholog Locus:PA0502
Sequence Length:723
Protein Length:240
Start: 571979
Stop: 572701
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03891.1|AE004487_5 (AE004487) probable biotin biosynthesis protein bioH [Pseudomonas aeruginosa PAO1]
Identities = 238/240 (99%), Positives = 239/240 (99%)
Sequence:
ATGCGTGACCACCTGATCCTGTTGCCGGGCTGGGGCCTGGGCAGCGCACCGCTGGAACCGCTGCGCGACGCG
CTGCACGAGCGCGAGCCGCACCTGAACGTGTTGATCGAGCCGCTGCCGTCGCTGGACGATGCCGCCGACTGG
CTCGACGAACTCGACGACAACCTGCCGCGCGATAGCTGGCTGGCCGGCTGGTCGCTGGGTGGCATGCTCGCC
GGCGAACTGGCGGCGCGCCGCGGCGACGATTGCCGGGGCCTGCTGACCCTGGCCAGCAATCCGTGCTTCCGT
GTGCGCGAGGACTGGCCGAACGCGATGCCGGCGGAAACCTTCGAGGACTTCTTCGAGGCTTTCCTGCTCGAA
CCGCACCTGACCCGCAAGCGTTTCACCCTACTGGTCAGCCAGGGTGCGCGCGACCCTCGGACCCTGGCGCGG
CAACTGCAGGTGGCGCTGCCGCAGCTGGAGCGCGAGGCGCTGGTCGCCGGCCTGCAGTTGCTCGGCCAACTG
GATACCCGGGCCGCCCTGGAAAACTTCCGCGGGCCGCAATTGCATCTGTTCGCCGAAGCCGATGCGCTGGTG
CCGGTGGCCGCCGCCGAGGCCCTGCTCGAGTGGCTGCCGGACGTCGAGGTCTCGACCCTGGCGGCCAGCCAC
GGCCTGCCGCTGGAATGCCCGGACGAGGTGGCCAGCGCAATCCTGAGATTCCTTCGCGAGGGTGACGATGCC
TGA
Translation:
MRDHLILLPGWGLGSAPLEPLRDALHEREPHLNVLIEPLPSLDDAADWLDELDDNLPRDSWLAGWSLGGMLA
GELAARRGDDCRGLLTLASNPCFRVREDWPNAMPAETFEDFFEAFLLEPHLTRKRFTLLVSQGARDPRTLAR
QLQVALPQLEREALVAGLQLLGQLDTRAALENFRGPQLHLFAEADALVPVAAAEALLEWLPDVEVSTLAASH
GLPLECPDEVASAILRFLREGDDA*
AnnotationID:3316GeneID:3308
AnnotatorUID: diggins
Modification Date/Time: 2005-03-28 04:04:47
Gene Name:
Confidence Code:3
GeneProduct:putative biotin biosynthesis protein bioH
Cell Localization:()
Synonyms:bioH
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:putative hydrolase
Functional Category Confidence Code:5
COGs:COG0596
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54040.1| (AE016857) bioH protein [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 243

 Score =  274 bits (700), Expect = 4e-73
 Identities = 147/243 (60%), Positives = 171/243 (70%), Gaps = 4/243 (1%)

>gb|AAN65995.1|AE016228_8 (AE016775) biotin biosynthesis protein BioH [Pseudomonas putida
           KT2440]
          Length = 243

 Score =  242 bits (617), Expect = 2e-63
 Identities = 131/243 (53%), Positives = 158/243 (65%), Gaps = 4/243 (1%)








 
Structural Features:
COG0596, MhpC, Predicted hydrolases or acyltransferases 
(alpha/beta hydrolase superfamily)
CD-Length = 282 residues,  91.1% aligned
Score = 61.6 bits (147), Expect = 7e-11
Identities = 69/257 (26%), Positives = 87/257 (33%), Gaps = 26/257 (10%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6619




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23761




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33813




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3308_cdd.html


    		




 
URL:

















    ReferenceID:35322




  
Author/Investigator(s):
        O'Regan M, Gloeckler R, Bernard S, Ledoux C, Ohsawa I, Lemoine Y.





  
Title:
        Nucleotide sequence of the bioH gene of Escherichia coli.




  
PubMed:
        2678009


MedLine:
        




    
Source:
Nucleic Acids Res. 1989 Oct 11;17(19):8004.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2678009

















    ReferenceID:35323




  
Author/Investigator(s):
        Akatsuka H, Kawai E, Sakurai N, Omori K.





  
Title:
        The Serratia marcescens bioH gene encodes an esterase.




  
PubMed:
        12527210


MedLine:
        




    
Source:
Gene. 2003 Jan 2;302(1-2):185-92.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12527210

















    ReferenceID:35324




  
Author/Investigator(s):
        Kiyasu T, Nagahashi Y, Hoshino T.





  
Title:
        Cloning and characterization of biotin biosynthetic genes of Kurthia sp.




  
PubMed:
        11255013


MedLine:
        




    
Source:
Gene. 2001 Mar 7;265(1-2):103-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11255013









           
Homologs By Global Alignment 

           

       



     
Gene ID:3308
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5306
                           PA0502_tr
                           translation of PA0502
                           240
                           99.16
                           99.58
                           0
                           1244.0
     

     
     
148473
                           gb|AAG03891.1|AE004487_5
                           (AE004487) probable biotin biosynthesis protein bioH [Pseudomonas aeruginosa PAO1]
                           240
                           99.16
                           99.58
                           0
                           1244.0
     

     
     
148474
                           gb|AAO54040.1|
                           (AE016857) bioH protein [Pseudomonas syringae pv. tomato str. DC3000]
                           244
                           60.24
                           70.08
                           5
                           706.0
     

     
     
148475
                           gb|AAN65995.1|AE016228_8
                           (AE016775) biotin biosynthesis protein BioH [Pseudomonas putida KT2440]
                           244
                           53.68
                           64.75
                           5
                           623.0
     

     






      

          
GC ORFID: 46310How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0502Glimmer Score: 

          
Start: 6373321Stop: 6374043

          
Length: 723


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0502 translation of PA0502Homolog Bit Score 483.0

          
Other Homologs:  t_PA0502 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGTGACCACCTGATCCTGTTGCCGGGCTGGGGCCTGGGCAGCGCACCGCTGGAACCGCTGCGCGACGCG 
CTGCACGAGCGCGAGCCGCACCTGAACGTGTTGATCGAGCCGCTGCCGTCGCTGGACGATGCCGCCGACTGG 
CTCGACGAACTCGACGACAACCTGCCGCGCGATAGCTGGCTGGCCGGCTGGTCGCTGGGTGGCATGCTCGCC 
GGCGAACTGGCGGCGCGCCGCGGCGACGATTGCCGGGGCCTGCTGACCCTGGCCAGCAATCCGTGCTTCCGT 
GTGCGCGAGGACTGGCCGAACGCGATGCCGGCGGAAACCTTCGAGGACTTCTTCGAGGCTTTCCTGCTCGAA 
CCGCACCTGACCCGCAAGCGTTTCACCCTACTGGTCAGCCAGGGTGCGCGCGACCCTCGGACCCTGGCGCGG 
CAACTGCAGGTGGCGCTGCCGCAGCTGGAGCGCGAGGCGCTGGTCGCCGGCCTGCAGTTGCTCGGCCAACTG 
GATACCCGGGCCGCCCTGGAAAACTTCCGCGGGCCGCAATTGCATCTGTTCGCCGAAGCCGATGCGCTGGTG 
CCGGTGGCCGCCGCCGAGGCCCTGCTCGAGTGGCTGCCGGACGTCGAGGTCTCGACCCTGGCGGCCAGCCAC 
GGCCTGCCGCTGGAATGCCCGGACGAGGTGGCCAGCGCAATCCTGAGATTCCTTCGCGAGGGTGACGATGCC 
TGA