ViewGene
Locus Name:
GeneID:
Location:

GeneID:3305
TrivialName: PA14_68040
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_68040
PAO1 Ortholog Locus:PA5150
Sequence Length:738
Protein Length:245
Start: 6071600
Stop: 6070863
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08535.1|AE004928_1 (AE004928) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 241/245 (98%), Positives = 242/245 (98%)
Sequence:
ATGGCGGAGAGAAAGACCCTGTTGCTGACCGGGGCGAGCCGCGGTATCGGGCATGCCACGGTGAAGTACTTC
AATGCCGCGGGTTGGCGGGTGTTCACCGCGTCGCGGCAGAGCTGGGCCAGCGAATGTCCCTGGGCGGACGGC
GAGGAAAACCACATCCACCTGGACCTGGAGGACATTCCCGGCGTCGAGGGCAGCCTGGAACTGATCCGCGAG
AAGCTCGGCGACGGTCGGCTCCACGCACTGGTGGACAATGCCGGCATCTCGCCCAAGGGCGAAGACGGCGCG
CGCCTCGGTGTGCTGGAAACCGACTACGCCACCTGGCTGCGGGTGTTCAACGTCAACCTGTTCTCCACCGCG
CTGCTGGCGCGCGGCCTGTTCGCCGAGCTGAAGGCGGCGCAGGGAACGGTGATAAACGTGACCTCCATCGCC
GGCTCGAAGGTGCATCCGTTCGCCGGGGTGGCCTACGCCACTTCCAAGGCGGCGTTGTCCGCGCTGACCCGC
GAGATGGCCCACGACTTCGGCCCGCATGGGGTGCGGGTGAACGCCATCGCGCCGGGGGAGATCGACACCGCG
ATCCTGTCGCCGGGCACCGAGGAGATCGTCGAGCGCAGCATCCCGCTGCACCGCCTCGGCCGGCCGGAGGAG
ATCGCTTCGCTGATCTACTTCCTCTGCACCAGCGGCGCATCCTACGTGAATGGCGCGGAAATCCACGTCAAC
GGCGGGCAGCACGTCTAG
Translation:
MAERKTLLLTGASRGIGHATVKYFNAAGWRVFTASRQSWASECPWADGEENHIHLDLEDIPGVEGSLELIRE
KLGDGRLHALVDNAGISPKGEDGARLGVLETDYATWLRVFNVNLFSTALLARGLFAELKAAQGTVINVTSIA
GSKVHPFAGVAYATSKAALSALTREMAHDFGPHGVRVNAIAPGEIDTAILSPGTEEIVERSIPLHRLGRPEE
IASLIYFLCTSGASYVNGAEIHVNGGQHV*
AnnotationID:3313GeneID:3305
AnnotatorUID: diggins
Modification Date/Time: 2005-03-15 13:01:36
Gene Name:
Confidence Code:3
GeneProduct:putative short-chain alcohol dehydrogenase
Cell Localization:()
Synonyms:fixR
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00106,COG1028,COG4221,COG0300
Secondary Functional Category(ies):Secondary metabolites biosynthesis, transport, and catabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57539.1| (AE016870) oxidoreductase, short chain dehydrogenase/reductase
           family [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 244

 Score =  405 bits (1041), Expect = e-112
 Identities = 197/245 (80%), Positives = 223/245 (91%), Gaps = 1/245 (0%)

>dbj|BAC47888.1| (AP005944) short-chain alcohol dehydrogenase [Bradyrhizobium
           japonicum USDA 110]
          Length = 250

 Score =  330 bits (845), Expect = 6e-90
 Identities = 161/244 (65%), Positives = 195/244 (79%)

>gb|AAL53013.1| (AE009616) FIXR PROTEIN [Brucella melitensis 16M]
          Length = 279

 Score =  321 bits (822), Expect = 3e-87
 Identities = 159/245 (64%), Positives = 193/245 (78%), Gaps = 3/245 (1%)








 
Structural Features:
pfam00106, adh_short, short chain dehydrogenase.
CD-Length = 247 residues,  99.6% aligned
Score =  159 bits (404), Expect = 3e-40
Identities = 95/255 (37%), Positives = 130/255 (50%), Gaps = 28/255 (10%)

COG1028, FabG, Dehydrogenases with different specificities 
(related to short-chain alcohol dehydrogenases) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 251 residues,  99.2% aligned
Score =  141 bits (355), Expect = 9e-35
Identities = 88/259 (33%), Positives = 123/259 (47%), Gaps = 26/259 (10%)

COG4221, COG4221, Short-chain alcohol dehydrogenase of unknown specificity 
CD-Length = 246 residues,  96.3% aligned
Score =  106 bits (265), Expect = 3e-24
Identities = 71/247 (28%), Positives = 113/247 (45%), Gaps = 18/247 (7%)

COG0300, DltE, Short-chain dehydrogenases of various substrate specificities
CD-Length = 265 residues,  86.8% aligned
Score = 92.6 bits (230), Expect = 4e-20
Identities = 56/240 (23%), Positives = 94/240 (39%), Gaps = 17/240 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6613




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23755




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34886




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3305_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3305
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5303
                           PA5150_tr
                           translation of PA5150
                           245
                           98.36
                           98.77
                           0
                           1242.0
     

     
     
148443
                           gb|AAG08535.1|AE004928_1
                           (AE004928) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           245
                           98.36
                           98.77
                           0
                           1242.0
     

     
     
148444
                           gb|AAO57539.1|
                           (AE016870) oxidoreductase, short chain dehydrogenase/reductase family [Pseudomonas syringae pv. tomato str. DC3000]
                           245
                           80.40
                           91.02
                           1
                           1043.0
     

     
     
148445
                           dbj|BAC47888.1|
                           (AP005944) short-chain alcohol dehydrogenase [Bradyrhizobium japonicum USDA 110]
                           250
                           64.40
                           78.00
                           5
                           843.0
     

     
     
148452
                           dbj|BAB50175.1|
                           (AP003001) short-chain dehydrogenase [Mesorhizobium loti]
                           251
                           63.74
                           78.48
                           7
                           803.0
     

     






      

          
GC ORFID: 46307How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5150Glimmer Score: 

          
Start: 5335286Stop: 5334549

          
Length: 738


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA5150 translation of PA5150Homolog Bit Score 483.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7639 (ORF: BLASTX 404.0), t_PA5150 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGGAGAGAAAGACCCTGTTGCTGACCGGGGCGAGCCGCGGTATCGGGCATGCCACGGTGAAGTACTTC 
AATGCCGCGGGTTGGCGGGTGTTCACCGCGTCGCGGCAGAGCTGGGCCAGCGAATGTCCCTGGGCGGACGGC 
GAGGAAAACCACATCCACCTGGACCTGGAGGACATTCCCGGCGTCGAGGGCAGCCTGGAACTGATCCGCGAG 
AAGCTCGGCGACGGTCGGCTCCACGCACTGGTGGACAATGCCGGCATCTCGCCCAAGGGCGAAGACGGCGCG 
CGCCTCGGTGTGCTGGAAACCGACTACGCCACCTGGCTGCGGGTGTTCAACGTCAACCTGTTCTCCACCGCG 
CTGCTGGCGCGCGGCCTGTTCGCCGAGCTGAAGGCGGCGCAGGGAACGGTGATAAACGTGACCTCCATCGCC 
GGCTCGAAGGTGCATCCGTTCGCCGGGGTGGCCTACGCCACTTCCAAGGCGGCGTTGTCCGCGCTGACCCGC 
GAGATGGCCCACGACTTCGGCCCGCATGGGGTGCGGGTGAACGCCATCGCGCCGGGGGAGATCGACACCGCG 
ATCCTGTCGCCGGGCACCGAGGAGATCGTCGAGCGCAGCATCCCGCTGCACCGCCTCGGCCGGCCGGAGGAG 
ATCGCTTCGCTGATCTACTTCCTCTGCACCAGCGGCGCATCCTACGTGAATGGCGCGGAAATCCACGTCAAC 
GGCGGGCAGCACGTCTAG