ViewGene
Locus Name:
GeneID:
Location:

GeneID:330
TrivialName: PA14_18350
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18350
PAO1 Ortholog Locus:PA3554
Sequence Length:1989
Protein Length:662
Start: 1576108
Stop: 1574120
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06942.1|AE004776_5 (AE004776) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 659/662 (99%), Positives = 660/662 (99%)
Sequence:
ATGACCTCGAAAGCCGTCGTCTTCGCCTACCACGACATCGGTTGCACCGGTATCGAAGCCCTGCTCAATGCC
GGCTACGAGATCGCCGCCGTCTTCACCCATGCCGACGACCCACGGGAAAACACCTTCTACGCCTCGGTCGCA
CGCCTCTGTGCCGAGCGCGGCATTCCGCTGCACGCGCCCGAGGACGTGAACCATCCGCTGTGGCTGGAGCGC
ATCCGCCAACTGCGCCCGGACTTCCTGTTCTCCTTCTACTACCGCCGCCTGCTCGGCGCCGAGCTGCTCGCC
TGCGCCGCACGCGGCGCCTACAACCTGCACGGTTCGCTGCTGCCGCGCTACCGCGGACGCGCCCCGGCGAAC
TGGGTGCTGGTCAACGGCGAAACGCAGACCGGGGTGACCCTGCATCGCATGATCGAGCGCGCCGACGCCGGG
CCGATCCTCGCCCAGCAGGCCGTCGCCATCGACCCCGAGGACACCGCCCTGAGCCTGCACGGCAAGCTGCGC
AAGGCCGCCGGCGCCCTGCTGCGCGACAGCCTGCCGCTGCTCGCCCTCGGCGTGCTGCCGGAAGTCGAGCAG
GACGAGAGCCAGGCCAGCCACTTCGGCCGGCGCACCCCGGCGGACGGCCTGCTCGACTGGCACAGGCCGGCA
CGGCAGTTGTACGACCTGGTGCGCGCGGTGACCCAGCCCTACCCTGGCGCCTTCTGCCAGGTCGGCGAACAG
AAGCTGATCGTCTGGAGCGCCGAGGTGGTCGCCGGCAACCACGGTCGCGAGCCGGGCAGCGTACTGTCCTGC
GACCCGCTGCGGATCGCCTGCGGCGAGGACTCGCTGGTGCTGCGCTTCGGCCAGCGCGGCGAGCGCGGCCTG
TACCTGGCCGGCACGCAACTGGCCACCGAGCTGGGCCTGGTCGAGGGCGCGCGCCTGCGTGGCGCGGCGTCC
GGCCCGCAGCGCCGCACGCGGGTGCTGATCCTCGGGGTCAACGGCTTCATCGGCAACCACCTGTCCGAACGC
CTGCTGCGCGACGGGCGCTACGAGGTCCACGGCATGGACATCGGCTCCGACGCCATCGAACGGCTCAAGGCC
GACCCGCATTTCCACTTCGTCGAAGGCGACATCGGCATCCATTCGGAGTGGCTCGAATACCATGTGAAGAAA
TGCGACGTGGTCCTGCCGCTGGTGGCCATCGCCACGCCCATCGAGTACACGCGCAACCCGCTGCGGGTGTTC
GAACTGGACTTCGAGGAAAACCTGCGGATCGTCCGCTACTGCGTGAAATACGGCAAACGCGTGGTGTTCCCT
TCCACCTCCGAGGTCTACGGCATGTGCCAGGACCCGGACTTCGACGAAGACCGCTCGAATCTGGTGGTCGGG
CCGATCAACAAGCAGCGCTGGATCTACTCGGTGTCCAAGCAGTTGCTCGACCGGGTGATCTGGGCCTACGGC
CAGCAGGGCCTGCGCTTCACCCTGTTCCGTCCGTTCAACTGGATGGGCCCGCGCCTGGACCGCCTGGATTCG
GCGCGGATCGGCAGCTCGCGGGCGATCACCCAGCTCATCCTGCACCTGGTCGAAGGCACGCCGATCCGCCTG
GTCGACGGCGGCGCGCAGAAGCGCTGCTTCACCGACGTCGACGACGGCATCGAGGCCCTCGCGCGGATCATC
GACAACCGCGACGGCCGCTGCGACGGGCAGATCGTCAACATCGGCAACCCGGACAACGAGGCGAGCATCCGC
CAGCTCGGCGAGGAACTGCTGCGCCAGTTCGAGGCCCACCCGCTGCGCGCGCAGTTCCCGCCCTTCGCCGGC
TTCCGCGAGGTGGAGAGCCGCAGCTTCTACGGCGACGGCTACCAGGACGTGGCCCACCGCAAGCCGAGCATC
GACAACGCCCGGCGCCTGCTCGACTGGCAGCCCACCATCGAACTGCGCGAGACCATCGGCAAGACCCTCGAC
TTCTTCCTCCACGAAGCGCTCCGCGAGCGCGAGGCACAGGCGTGA
Translation:
MTSKAVVFAYHDIGCTGIEALLNAGYEIAAVFTHADDPRENTFYASVARLCAERGIPLHAPEDVNHPLWLER
IRQLRPDFLFSFYYRRLLGAELLACAARGAYNLHGSLLPRYRGRAPANWVLVNGETQTGVTLHRMIERADAG
PILAQQAVAIDPEDTALSLHGKLRKAAGALLRDSLPLLALGVLPEVEQDESQASHFGRRTPADGLLDWHRPA
RQLYDLVRAVTQPYPGAFCQVGEQKLIVWSAEVVAGNHGREPGSVLSCDPLRIACGEDSLVLRFGQRGERGL
YLAGTQLATELGLVEGARLRGAASGPQRRTRVLILGVNGFIGNHLSERLLRDGRYEVHGMDIGSDAIERLKA
DPHFHFVEGDIGIHSEWLEYHVKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLRIVRYCVKYGKRVVFP
STSEVYGMCQDPDFDEDRSNLVVGPINKQRWIYSVSKQLLDRVIWAYGQQGLRFTLFRPFNWMGPRLDRLDS
ARIGSSRAITQLILHLVEGTPIRLVDGGAQKRCFTDVDDGIEALARIIDNRDGRCDGQIVNIGNPDNEASIR
QLGEELLRQFEAHPLRAQFPPFAGFREVESRSFYGDGYQDVAHRKPSIDNARRLLDWQPTIELRETIGKTLD
FFLHEALREREAQA*
AnnotationID:340GeneID:330
AnnotatorUID: diggins
Modification Date/Time: 2005-04-20 05:05:49
Gene Name:
Confidence Code:3
GeneProduct:putative transformylase
Cell Localization:()
Synonyms:fmt
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0223,COG0451
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL23678.1| (AY057445) UDP-D-glucuronate dehydrogenase [Escherichia coli]
          Length = 660

 Score =  928 bits (2398), Expect = 0.0
 Identities = 453/650 (69%), Positives = 532/650 (81%), Gaps = 3/650 (0%)

>dbj|BAA16082.1| (D90857) METHIONYL-TRNA FORMYLTRANSFERASE (EC 2.1.2.9).
           [Escherichia coli]
          Length = 660

 Score =  928 bits (2398), Expect = 0.0
 Identities = 453/650 (69%), Positives = 532/650 (81%), Gaps = 3/650 (0%)

>gb|AAL21200.1| (AE008803) putative transformylase [Salmonella typhimurium LT2]
          Length = 660

 Score =  919 bits (2375), Expect = 0.0
 Identities = 446/650 (68%), Positives = 531/650 (81%), Gaps = 3/650 (0%)






 
Structural Features:
COG0223, Fmt, Methionyl-tRNA formyltransferase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 307 residues,  99.3% aligned
Score =  325 bits (834), Expect = 1e-89
Identities = 123/307 (40%), Positives = 176/307 (57%), Gaps = 7/307 (2%)

COG0451, WcaG, Nucleoside-diphosphate-sugar epimerases 
[Cell envelope biogenesis, outer membrane / 
Carbohydrate transport and metabolism]
CD-Length = 314 residues,  99.7% aligned
Score =  158 bits (401), Expect = 1e-39
Identities = 99/343 (28%), Positives = 150/343 (43%), Gaps = 35/343 (10%)






   
Genomic Context:






   
Comment:


















    ReferenceID:663




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17805




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32015




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:330_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:330
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
547
                           PA3554_tr
                           translation of PA3554
                           662
                           99.54
                           99.69
                           0
                           3465.0
     

     
     
26917
                           gb|AAG06942.1|AE004776_5
                           (AE004776) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           662
                           99.54
                           99.69
                           0
                           3465.0
     

     
     
26918
                           gb|AAN81251.1|AE016763_210
                           (AE016763) Hypothetical protein yfbG [Escherichia coli CFT073]
                           665
                           68.42
                           80.75
                           8
                           2401.0
     

     
     
26922
                           dbj|BAA16078.1|
                           (D90856) METHIONYL-TRNA FORMYLTRANSFERASE (EC 2.1.2.9). [Escherichia coli]
                           665
                           68.27
                           80.60
                           8
                           2399.0
     

     
     
26921
                           dbj|BAA16082.1|
                           (D90857) METHIONYL-TRNA FORMYLTRANSFERASE (EC 2.1.2.9). [Escherichia coli]
                           665
                           68.27
                           80.60
                           8
                           2399.0
     

     






      

          
GC ORFID: 43332How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3554Glimmer Score: 

          
Start: 3377827Stop: 3379815

          
Length: 1989


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3554 translation of PA3554Homolog Bit Score 1339.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6699 (ORF: BLASTX 1051.0), psyr_15may02_Scaffold6_revised_gene6700 (ORF: BLASTX 1051.0), t_PA3554 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCTCGAAAGCCGTCGTCTTCGCCTACCACGACATCGGTTGCACCGGTATCGAAGCCCTGCTCAATGCC 
GGCTACGAGATCGCCGCCGTCTTCACCCATGCCGACGACCCACGGGAAAACACCTTCTACGCCTCGGTCGCA 
CGCCTCTGTGCCGAGCGCGGCATTCCGCTGCACGCGCCCGAGGACGTGAACCATCCGCTGTGGCTGGAGCGC 
ATCCGCCAACTGCGCCCGGACTTCCTGTTCTCCTTCTACTACCGCCGCCTGCTCGGCGCCGAGCTGCTCGCC 
TGCGCCGCACGCGGCGCCTACAACCTGCACGGTTCGCTGCTGCCGCGCTACCGCGGACGCGCCCCGGCGAAC 
TGGGTGCTGGTCAACGGCGAAACGCAGACCGGGGTGACCCTGCATCGCATGATCGAGCGCGCCGACGCCGGG 
CCGATCCTCGCCCAGCAGGCCGTCGCCATCGACCCCGAGGACACCGCCCTGAGCCTGCACGGCAAGCTGCGC 
AAGGCCGCCGGCGCCCTGCTGCGCGACAGCCTGCCGCTGCTCGCCCTCGGCGTGCTGCCGGAAGTCGAGCAG 
GACGAGAGCCAGGCCAGCCACTTCGGCCGGCGCACCCCGGCGGACGGCCTGCTCGACTGGCACAGGCCGGCA 
CGGCAGTTGTACGACCTGGTGCGCGCGGTGACCCAGCCCTACCCTGGCGCCTTCTGCCAGGTCGGCGAACAG 
AAGCTGATCGTCTGGAGCGCCGAGGTGGTCGCCGGCAACCACGGTCGCGAGCCGGGCAGCGTACTGTCCTGC 
GACCCGCTGCGGATCGCCTGCGGCGAGGACTCGCTGGTGCTGCGCTTCGGCCAGCGCGGCGAGCGCGGCCTG 
TACCTGGCCGGCACGCAACTGGCCACCGAGCTGGGCCTGGTCGAGGGCGCGCGCCTGCGTGGCGCGGCGTCC 
GGCCCGCAGCGCCGCACGCGGGTGCTGATCCTCGGGGTCAACGGCTTCATCGGCAACCACCTGTCCGAACGC 
CTGCTGCGCGACGGGCGCTACGAGGTCCACGGCATGGACATCGGCTCCGACGCCATCGAACGGCTCAAGGCC 
GACCCGCATTTCCACTTCGTCGAAGGCGACATCGGCATCCATTCGGAGTGGCTCGAATACCATGTGAAGAAA 
TGCGACGTGGTCCTGCCGCTGGTGGCCATCGCCACGCCCATCGAGTACACGCGCAACCCGCTGCGGGTGTTC 
GAACTGGACTTCGAGGAAAACCTGCGGATCGTCCGCTACTGCGTGAAATACGGCAAACGCGTGGTGTTCCCT 
TCCACCTCCGAGGTCTACGGCATGTGCCAGGACCCGGACTTCGACGAAGACCGCTCGAATCTGGTGGTCGGG 
CCGATCAACAAGCAGCGCTGGATCTACTCGGTGTCCAAGCAGTTGCTCGACCGGGTGATCTGGGCCTACGGC 
CAGCAGGGCCTGCGCTTCACCCTGTTCCGTCCGTTCAACTGGATGGGCCCGCGCCTGGACCGCCTGGATTCG 
GCGCGGATCGGCAGCTCGCGGGCGATCACCCAGCTCATCCTGCACCTGGTCGAAGGCACGCCGATCCGCCTG 
GTCGACGGCGGCGCGCAGAAGCGCTGCTTCACCGACGTCGACGACGGCATCGAGGCCCTCGCGCGGATCATC 
GACAACCGCGACGGCCGCTGCGACGGGCAGATCGTCAACATCGGCAACCCGGACAACGAGGCGAGCATCCGC 
CAGCTCGGCGAGGAACTGCTGCGCCAGTTCGAGGCCCACCCGCTGCGCGCGCAGTTCCCGCCCTTCGCCGGC 
TTCCGCGAGGTGGAGAGCCGCAGCTTCTACGGCGACGGCTACCAGGACGTGGCCCACCGCAAGCCGAGCATC 
GACAACGCCCGGCGCCTGCTCGACTGGCAGCCCACCATCGAACTGCGCGAGACCATCGGCAAGACCCTCGAC 
TTCTTCCTCCACGAAGCGCTCCGCGAGCGCGAGGCACAGGCGTGA