ViewGene
Locus Name:
GeneID:
Location:

GeneID:329
TrivialName: PA14_63640
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_63640
PAO1 Ortholog Locus:PA4814
Sequence Length:2046
Protein Length:681
Start: 5673945
Stop: 5675990
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08199.1|AE004894_5 (AE004894) 2,4-dienoyl-CoA reductase FadH2 [Pseudomonas aeruginosa PAO1]
Identities = 671/681 (98%), Positives = 674/681 (98%)
Sequence:
ATGCGCAACCCCTACCCGCCGCTCGCCGCCGCTCCCTATCCGCACCTGCTGGCCCCGCTGGACCTGGGCTTC
ACCCGTCTGCGCAACCGCGTGGTGATGGGTTCGATGCACACCGGGCTGGAGGATCGTCTGTGGGACTTCGGC
AAGCTCGCTGCCTACTACCGCGAGCGCGCCCGGGGCGGCGCCGGGCTGATCGTCACCGGCGGCTTCGCGCCC
TGCCGCGAAGGTTGGCTGCTGCCCTTCGGCAGCAGCCTCACCAGCGCCCTGGAGGTGCCCGCCCATCGTCGC
CTGACCCAGGCGGTGCATGCCGAGGGCGGCAAGATCCTGCTGCAGATCCTCCACGCCGGGCGCTATGGCTAC
CACCCGATGGCGGTGTCCGCCTCGCCGTTGAAGTCGCCGATCAGTTGGTTCACCCCGCGCGAACTCTCCGAG
CGTGGCATCCGCAAGACCATCCGCGCCTTCGTCCGTTGCGCGCGGCTGGCCAAGGCCGCCGCCTACGACGGC
GTGGAAGTGATGGGCAGCGAAGGCTACCTGCTCAACCAGTTCCTCTGCCCGCGCACCAACCGCCGCGACGAC
CAGTGGGGCGGCCCGCTGGAAAACCGCATGCGCCTGGCGCTGGAGATCGTCCGCGGCATCCGGCGGATCGTC
GGCGAACGCTTCATCCTCTGCTATCGCCTGTCGCTGCTCGACCTGGTCGAGGGCGGCAATACCTGGGACGAA
GTACGGCGCATCGCCCTGGCCCTGGAGGAAGCCGGCATCACCCTGCTCAACACCGGTATCGGCTGGCACGAG
TCGCGCGTGCCGACCATCGTCACCTCGGTGCCGCGCGCGGCCTTCGCCGAGGTCAGCGCACGGCTGCGCCAC
GAACTGAAGGTGCCGGTGATCGCCAGCAACCGGATCAACACCCCGGAGGTCGCCGAATCCCTGCTCGCCGCC
GGCAAGGCCGACCTGGTATCGATGGCCCGGCCGTTGCTGGCCGACCCGCAGTTCGTGGCCAAGGCCGGTGCC
GGCCGGGCCGAGGAGATCAACACCTGCATCGCCTGCAACCAGGCCTGCCTCGATCATGCCTTCGCCAACCGC
CGCGCCACCTGCCTGGTCAACCCGCGGGCGGCCTTCGAGACCGAGTTGCGCTACCGCAAGGCGCGCACGCAG
AAGCGCATCGCGGTGATCGGCGCCGGCCCCGCCGGGCTCTCCGCCGCCTGCGTGGCCGCCGAGCGCGGGCAT
CGGGTCAGCCTGTTCGAGGCGAGCGGGGAAATCGGCGGGCAGTTCAACCTGGCCAAGCGGATTCCCGGCAAG
GAAGAGTTCCGCGAAACCCTGCGCTACTTCCGCGTGCGCCTGGAACGCCTCGGCGTCGACCTGCGCCTCGGC
CACCGGGTGCGCCAGGGCGAGCTGGACGGTCAGTTCGACGACGTGGTGGTGGCCACCGGCATCCAGCCACGC
CGGCCAAGGATCGACGGCATCGGCGGACCGACCGTACTCAGCTACGTGGACGTGCTGCGCGGCGCGCCGGTG
GGCGCGCGGGTCGCCATCGTCGGCGCCGGCGGCATCGGCTTCGACGTCGCCGCCTTCCTCGTCGCCGCACCC
AGCGACGGCCAGCCGCGGGCGCTCGGCGAATGGCTCGCGGAATGGGGCGTGGACCTCGACAACAGCCAGCCC
GGCGGCCTCCGCGAACCCGCTCCGACGCAGCCGGCGCGCCAGGTCTGGCTGCTCCAGCGCAAGCCCGGCGCC
CCCGGCGCGCAGCTCGGCAAGACCAGCGGCTGGGTGCACCGCGCGCACCTCAGGCACAACGCCGTGCGCATG
CTCGGCGGGGTGGAGTACCTGAAGATCGACGAACGCGGCTTGTTGATCCGCGTCGACGGCGAGGAACGCTGG
CTGGAGGTGGAGAACGTGGTGATCTGCGCCGGCCAGGAGCCGCTGCGCGAGTTGCAGATCAGCCAGGCGGCG
GAAAGCCTGCGCTTCCACCTGATCGGCGGGGCGCGGGTGGCGGGGGAACTGGATGCCAAGCGGGCAATTCGC
GAGGGGGCCATGCTGGCGGCGCGCTTGTAG
Translation:
MRNPYPPLAAAPYPHLLAPLDLGFTRLRNRVVMGSMHTGLEDRLWDFGKLAAYYRERARGGAGLIVTGGFAP
CREGWLLPFGSSLTSALEVPAHRRLTQAVHAEGGKILLQILHAGRYGYHPMAVSASPLKSPISWFTPRELSE
RGIRKTIRAFVRCARLAKAAAYDGVEVMGSEGYLLNQFLCPRTNRRDDQWGGPLENRMRLALEIVRGIRRIV
GERFILCYRLSLLDLVEGGNTWDEVRRIALALEEAGITLLNTGIGWHESRVPTIVTSVPRAAFAEVSARLRH
ELKVPVIASNRINTPEVAESLLAAGKADLVSMARPLLADPQFVAKAGAGRAEEINTCIACNQACLDHAFANR
RATCLVNPRAAFETELRYRKARTQKRIAVIGAGPAGLSAACVAAERGHRVSLFEASGEIGGQFNLAKRIPGK
EEFRETLRYFRVRLERLGVDLRLGHRVRQGELDGQFDDVVVATGIQPRRPRIDGIGGPTVLSYVDVLRGAPV
GARVAIVGAGGIGFDVAAFLVAAPSDGQPRALGEWLAEWGVDLDNSQPGGLREPAPTQPARQVWLLQRKPGA
PGAQLGKTSGWVHRAHLRHNAVRMLGGVEYLKIDERGLLIRVDGEERWLEVENVVICAGQEPLRELQISQAA
ESLRFHLIGGARVAGELDAKRAIREGAMLAARL*
AnnotationID:339GeneID:329
AnnotatorUID: diggins
Modification Date/Time: 2005-04-25 20:08:39
Gene Name:fadH2
Confidence Code:2
GeneProduct:2,4-dienoyl-CoA reductase FadH2
Cell Localization:()
Synonyms:fadH
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1902,pfam00724
Secondary Functional Category(ies):energy metabolism
EC Number:1.3.1.34
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM40243.1| (AE012191) 2,4-dienoyl-CoA reductase [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 679

 Score =  820 bits (2119), Expect = 0.0
 Identities = 422/683 (61%), Positives = 511/683 (74%), Gaps = 9/683 (1%)

>gb|AAM35893.1| (AE011730) 2,4-dienoyl-CoA reductase [Xanthomonas axonopodis pv.
           citri str. 306]
          Length = 679

 Score =  814 bits (2103), Expect = 0.0
 Identities = 419/676 (61%), Positives = 503/676 (74%), Gaps = 7/676 (1%)

>gb|AAN67622.1|AE016392_3 (AE016781) 2,4-dienoyl-CoA reductase FadH [Pseudomonas putida
           KT2440]
          Length = 693

 Score =  794 bits (2050), Expect = 0.0
 Identities = 416/680 (61%), Positives = 503/680 (73%), Gaps = 8/680 (1%)






 
Structural Features:
COG1902, NemA, NADH:flavin oxidoreductases, Old Yellow Enzyme family 
[Energy production and conversion]
CD-Length = 363 residues, 100.0% aligned
Score =  371 bits (954), Expect = 1e-103
Identities = 161/364 (44%), Positives = 201/364 (55%), Gaps = 9/364 (2%)

pfam00724, Oxidored_FMN, NADH:flavin oxidoreductase / NADH oxidase family. 
CD-Length = 335 residues,  99.7% aligned
Score =  272 bits (696), Expect = 1e-73
Identities = 127/336 (37%), Positives = 183/336 (54%), Gaps = 12/336 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:661




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17803




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35235




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:329_cdd.html


    		




 
URL:

















    ReferenceID:35986




  
Author/Investigator(s):
        He XY, Yang SY, Schulz H.





  
Title:
        Cloning and expression of the fadH gene and characterization of the gene product 2,4-dienoyl coenzyme A reductase from Escherichia coli.




  
PubMed:
        9346310


MedLine:
        




    
Source:
Eur J Biochem. 1997 Sep 1;248(2):516-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9346310









           
Homologs By Global Alignment 

           

       



     
Gene ID:329
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
545
                           PA4814_tr
                           translation of PA4814
                           681
                           98.53
                           98.97
                           0
                           3469.0
     

     
     
26883
                           gb|AAG08199.1|AE004894_5
                           (AE004894) 2,4-dienoyl-CoA reductase FadH2 [Pseudomonas aeruginosa PAO1]
                           681
                           98.53
                           98.97
                           0
                           3469.0
     

     
     
26884
                           gb|AAM40243.1|
                           (AE012191) 2,4-dienoyl-CoA reductase [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           685
                           61.60
                           74.59
                           10
                           2128.0
     

     
     
26885
                           gb|AAM35893.1|
                           (AE011730) 2,4-dienoyl-CoA reductase [Xanthomonas axonopodis pv. citri str. 306]
                           684
                           61.40
                           73.83
                           8
                           2111.0
     

     
     
26886
                           gb|AAN67622.1|AE016392_3
                           (AE016781) 2,4-dienoyl-CoA reductase FadH [Pseudomonas putida KT2440]
                           695
                           60.43
                           72.94
                           16
                           2064.0
     

     






      

          
GC ORFID: 43331How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4814Glimmer Score: 

          
Start: 4937625Stop: 4939670

          
Length: 2046


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4814 translation of PA4814Homolog Bit Score 1340.0

          
Other Homologs:  t_PA3092 (ORF: BLASTX 788.0), t_PA4814 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCAACCCCTACCCGCCGCTCGCCGCCGCTCCCTATCCGCACCTGCTGGCCCCGCTGGACCTGGGCTTC 
ACCCGTCTGCGCAACCGCGTGGTGATGGGTTCGATGCACACCGGGCTGGAGGATCGTCTGTGGGACTTCGGC 
AAGCTCGCTGCCTACTACCGCGAGCGCGCCCGGGGCGGCGCCGGGCTGATCGTCACCGGCGGCTTCGCGCCC 
TGCCGCGAAGGTTGGCTGCTGCCCTTCGGCAGCAGCCTCACCAGCGCCCTGGAGGTGCCCGCCCATCGTCGC 
CTGACCCAGGCGGTGCATGCCGAGGGCGGCAAGATCCTGCTGCAGATCCTCCACGCCGGGCGCTATGGCTAC 
CACCCGATGGCGGTGTCCGCCTCGCCGTTGAAGTCGCCGATCAGTTGGTTCACCCCGCGCGAACTCTCCGAG 
CGTGGCATCCGCAAGACCATCCGCGCCTTCGTCCGTTGCGCGCGGCTGGCCAAGGCCGCCGCCTACGACGGC 
GTGGAAGTGATGGGCAGCGAAGGCTACCTGCTCAACCAGTTCCTCTGCCCGCGCACCAACCGCCGCGACGAC 
CAGTGGGGCGGCCCGCTGGAAAACCGCATGCGCCTGGCGCTGGAGATCGTCCGCGGCATCCGGCGGATCGTC 
GGCGAACGCTTCATCCTCTGCTATCGCCTGTCGCTGCTCGACCTGGTCGAGGGCGGCAATACCTGGGACGAA 
GTACGGCGCATCGCCCTGGCCCTGGAGGAAGCCGGCATCACCCTGCTCAACACCGGTATCGGCTGGCACGAG 
TCGCGCGTGCCGACCATCGTCACCTCGGTGCCGCGCGCGGCCTTCGCCGAGGTCAGCGCACGGCTGCGCCAC 
GAACTGAAGGTGCCGGTGATCGCCAGCAACCGGATCAACACCCCGGAGGTCGCCGAATCCCTGCTCGCCGCC 
GGCAAGGCCGACCTGGTATCGATGGCCCGGCCGTTGCTGGCCGACCCGCAGTTCGTGGCCAAGGCCGGTGCC 
GGCCGGGCCGAGGAGATCAACACCTGCATCGCCTGCAACCAGGCCTGCCTCGATCATGCCTTCGCCAACCGC 
CGCGCCACCTGCCTGGTCAACCCGCGGGCGGCCTTCGAGACCGAGTTGCGCTACCGCAAGGCGCGCACGCAG 
AAGCGCATCGCGGTGATCGGCGCCGGCCCCGCCGGGCTCTCCGCCGCCTGCGTGGCCGCCGAGCGCGGGCAT 
CGGGTCAGCCTGTTCGAGGCGAGCGGGGAAATCGGCGGGCAGTTCAACCTGGCCAAGCGGATTCCCGGCAAG 
GAAGAGTTCCGCGAAACCCTGCGCTACTTCCGCGTGCGCCTGGAACGCCTCGGCGTCGACCTGCGCCTCGGC 
CACCGGGTGCGCCAGGGCGAGCTGGACGGTCAGTTCGACGACGTGGTGGTGGCCACCGGCATCCAGCCACGC 
CGGCCAAGGATCGACGGCATCGGCGGACCGACCGTACTCAGCTACGTGGACGTGCTGCGCGGCGCGCCGGTG 
GGCGCGCGGGTCGCCATCGTCGGCGCCGGCGGCATCGGCTTCGACGTCGCCGCCTTCCTCGTCGCCGCACCC 
AGCGACGGCCAGCCGCGGGCGCTCGGCGAATGGCTCGCGGAATGGGGCGTGGACCTCGACAACAGCCAGCCC 
GGCGGCCTCCGCGAACCCGCTCCGACGCAGCCGGCGCGCCAGGTCTGGCTGCTCCAGCGCAAGCCCGGCGCC 
CCCGGCGCGCAGCTCGGCAAGACCAGCGGCTGGGTGCACCGCGCGCACCTCAGGCACAACGCCGTGCGCATG 
CTCGGCGGGGTGGAGTACCTGAAGATCGACGAACGCGGCTTGTTGATCCGCGTCGACGGCGAGGAACGCTGG 
CTGGAGGTGGAGAACGTGGTGATCTGCGCCGGCCAGGAGCCGCTGCGCGAGTTGCAGATCAGCCAGGCGGCG 
GAAAGCCTGCGCTTCCACCTGATCGGCGGGGCGCGGGTGGCGGGGGAACTGGATGCCAAGCGGGCAATTCGC 
GAGGGGGCCATGCTGGCGGCGCGCTTGTAG