ViewGene
Locus Name:
GeneID:
Location:

GeneID:3287
TrivialName: PA14_61670
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_61670
PAO1 Ortholog Locus:PA4663
Sequence Length:759
Protein Length:252
Start: 5503602
Stop: 5502844
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08050.1|AE004880_6 (AE004880) molybdopterin biosynthesis MoeB protein [Pseudomonas aeruginosa PAO1]
Identities = 250/252 (99%), Positives = 250/252 (99%)
Sequence:
ATGCTGAGCGACGAGGAACTGCTGCGCTACAGTCGCCAGATCCTGCTGGCGCAGGTCGACGTCGAGGGCCAG
CTGCGCCTGAAGCGCAGCCGCGCACTGATCGTCGGTCTTGGCGGCCTGGGTTCGCCGGTGGCGCTGTACCTG
GCCGCGGCCGGGGTCGGCGAGCTGCACCTGGCGGATTTCGATACGGTCGACCTGACCAACCTGCAACGGCAG
GTCATCCACGATGGCGCCAGCGTGGGTATCGGCAAGGTGGAGTCGGCGATGCGCCGGCTGGGCGCACTGAAT
CCGCAGATCCGCCTGGTCAGCCATAGCGCCGCGCTGGACGAGGACTCCCTCGATGCCGCCGTGGCGGCGGTC
GACCTGGTGCTCGACTGCACCGACAACTTCGCCACCCGCGAGGCGGTCAACGTCGCCTGCGTGCGCGCCGGC
AAGCCGCTGGTCAGTGGCGCGGCGATCCGTCTTGAGGGCCAGCTTTCGGTATTCGATACCCGCTGCGACGCA
AGCCCCTGCTACCACTGCCTCTACGGGCACGGCAGCGAGGCCGAGCTGACCTGCAGCGAGGCCGGCGTGATC
GGTCCGCTGGTGGGGCTGGTCGGTAGCCTGCAGGCGCTGGAGGCGCTCAAGCTGCTGGCCGGTTTCGGCGAA
CCGCTGGTGGGCCGCCTGCTGCTGGTGGACGCCCTCGGCACGCGCTTCCGCGAATTGCGGGTGCGTCGCGAT
CCTTCCTGTGCCGTCTGCGGGCAACGCCATGGCGGTTGA
Translation:
MLSDEELLRYSRQILLAQVDVEGQLRLKRSRALIVGLGGLGSPVALYLAAAGVGELHLADFDTVDLTNLQRQ
VIHDGASVGIGKVESAMRRLGALNPQIRLVSHSAALDEDSLDAAVAAVDLVLDCTDNFATREAVNVACVRAG
KPLVSGAAIRLEGQLSVFDTRCDASPCYHCLYGHGSEAELTCSEAGVIGPLVGLVGSLQALEALKLLAGFGE
PLVGRLLLVDALGTRFRELRVRRDPSCAVCGQRHGG*
AnnotationID:3295GeneID:3287
AnnotatorUID: diggins
Modification Date/Time: 2005-03-08 06:06:50
Gene Name:moeB
Confidence Code:2
GeneProduct:molybdopterin biosynthesis MoeB protein
Cell Localization:()
Synonyms:chlN
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00757,COG0476,pfam00899
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66360.1|AE016264_4 (AE016776) molybdopterin biosynthesis MoeB protein, putative
           [Pseudomonas putida KT2440]
          Length = 251

 Score =  427 bits (1099), Expect = e-119
 Identities = 212/251 (84%), Positives = 236/251 (94%)

>gb|AAO54640.1| (AE016859) molybdopterin biosynthesis protein MoeB [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 259

 Score =  412 bits (1058), Expect = e-114
 Identities = 206/251 (82%), Positives = 228/251 (90%)

>gb|AAN79384.1|AE016757_288 (AE016757) Molybdopterin biosynthesis protein moeB [Escherichia
           coli CFT073]
          Length = 249

 Score =  271 bits (694), Expect = 2e-72
 Identities = 139/247 (56%), Positives = 177/247 (71%), Gaps = 1/247 (0%)








 
Structural Features:
cd00757, ThiF_MoeB_HesA_family, ThiF_MoeB_HesA.
CD-Length = 228 residues, 100.0% aligned
Score =  295 bits (757), Expect = 3e-81

COG0476, ThiF, Dinucleotide-utilizing enzymes involved in molybdopterin 
and thiamine biosynthesis family 2 [Coenzyme metabolism]
CD-Length = 254 residues,  99.6% aligned
Score =  253 bits (648), Expect = 1e-68
Identities = 145/254 (57%), Positives = 176/254 (69%), Gaps = 7/254 (2%)

pfam00899, ThiF, ThiF family.
CD-Length = 136 residues, 100.0% aligned
Score =  133 bits (335), Expect = 3e-32
Identities = 56/136 (41%), Positives = 78/136 (57%), Gaps = 1/136 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6577




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23719




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37595




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3287_cdd.html


    		




 
URL:

















    ReferenceID:37769




  
Author/Investigator(s):
        Solomon PS, Shaw AL, Lane I, Hanson GR, Palmer T, McEwan AG.





  
Title:
        Characterization of a molybdenum cofactor biosynthetic gene cluster in Rhodobacter capsulatus which is specific for the biogenesis of dimethylsulfoxide reductase.




  
PubMed:
        10411269


MedLine:
        




    
Source:
Microbiology. 1999 Jun;145 ( Pt 6):1421-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10411269

















    ReferenceID:37770




  
Author/Investigator(s):
        Nohno T, Kasai Y, Saito T.





  
Title:
        Cloning and sequencing of the Escherichia coli chlEN operon involved in molybdopterin biosynthesis.




  
PubMed:
        3045084


MedLine:
        




    
Source:
J Bacteriol. 1988 Sep;170(9):4097-102.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3045084









           
Homologs By Global Alignment 

           

       



     
Gene ID:3287
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5282
                           PA4663_tr
                           translation of PA4663
                           252
                           99.20
                           99.20
                           0
                           1248.0
     

     
     
148103
                           gb|AAG08050.1|AE004880_6
                           (AE004880) molybdopterin biosynthesis MoeB protein [Pseudomonas aeruginosa PAO1]
                           252
                           99.20
                           99.20
                           0
                           1248.0
     

     
     
148104
                           gb|AAN66360.1|AE016264_4
                           (AE016776) molybdopterin biosynthesis MoeB protein, putative [Pseudomonas putida KT2440]
                           252
                           84.12
                           93.65
                           1
                           1099.0
     

     
     
148105
                           gb|AAO54640.1|
                           (AE016859) molybdopterin biosynthesis protein MoeB [Pseudomonas syringae pv. tomato str. DC3000]
                           259
                           79.53
                           88.03
                           7
                           1056.0
     

     
     
148106
                           gb|AAQ61918.1|
                           (AE016925) molybdopterin biosynthesis MoeB protein [Chromobacterium violaceum ATCC 12472]
                           257
                           57.58
                           69.64
                           9
                           704.0
     

     






      

          
GC ORFID: 46289How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4663Glimmer Score: 

          
Start: 4767282Stop: 4766524

          
Length: 759


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4663 translation of PA4663Homolog Bit Score 485.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4298 (ORF: BLASTX 408.0), t_PA4663 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTGAGCGACGAGGAACTGCTGCGCTACAGTCGCCAGATCCTGCTGGCGCAGGTCGACGTCGAGGGCCAG 
CTGCGCCTGAAGCGCAGCCGCGCACTGATCGTCGGTCTTGGCGGCCTGGGTTCGCCGGTGGCGCTGTACCTG 
GCCGCGGCCGGGGTCGGCGAGCTGCACCTGGCGGATTTCGATACGGTCGACCTGACCAACCTGCAACGGCAG 
GTCATCCACGATGGCGCCAGCGTGGGTATCGGCAAGGTGGAGTCGGCGATGCGCCGGCTGGGCGCACTGAAT 
CCGCAGATCCGCCTGGTCAGCCATAGCGCCGCGCTGGACGAGGACTCCCTCGATGCCGCCGTGGCGGCGGTC 
GACCTGGTGCTCGACTGCACCGACAACTTCGCCACCCGCGAGGCGGTCAACGTCGCCTGCGTGCGCGCCGGC 
AAGCCGCTGGTCAGTGGCGCGGCGATCCGTCTTGAGGGCCAGCTTTCGGTATTCGATACCCGCTGCGACGCA 
AGCCCCTGCTACCACTGCCTCTACGGGCACGGCAGCGAGGCCGAGCTGACCTGCAGCGAGGCCGGCGTGATC 
GGTCCGCTGGTGGGGCTGGTCGGTAGCCTGCAGGCGCTGGAGGCGCTCAAGCTGCTGGCCGGTTTCGGCGAA 
CCGCTGGTGGGCCGCCTGCTGCTGGTGGACGCCCTCGGCACGCGCTTCCGCGAATTGCGGGTGCGTCGCGAT 
CCTTCCTGTGCCGTCTGCGGGCAACGCCATGGCGGTTGA