ViewGene
Locus Name:
GeneID:
Location:

GeneID:3284
TrivialName: PA14_31510
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_31510
PAO1 Ortholog Locus:PA2554
Sequence Length:768
Protein Length:255
Start: 2740053
Stop: 2740820
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05942.1|AE004683_6 (AE004683) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 254/255 (99%), Positives = 254/255 (99%)
Sequence:
GTGCGTATCGAAAATCGAGTGTTCCTGGTCACCGGCGGTAGCTCCGGGCTCGGCGCCGCCACCGCGAAGATG
CTGGTGGAGCAGGGCGGCAAGGTGGTGCTGGCCGACATCAACGCCGAGGCCGGCGCGGCCAAGGCCGCCGAG
CTTGGCGCCCAGGCGCGCTTCGTCAGGGCCGACATCGCCAGCGAGGCCGACGGCCGCCAGGCCGTGGCCACT
GCACTGGAGGCCTTCGGCGGCCTCCATGGCCTGGCCAATTGCGCGGGCGTGGCGCCGGCCGAGAAGGTCCTC
GGACGCAATGGCATCCATGCCCTGGAGAGTTTCCGCCGGGTCATCGACATCAACCTGGTGGGCAGCTTCAAC
ATGCTGCGCCTGGCCGCCGAGGCCATGTCCCAGGGCCAGCCGGACGAGGGCGGCGAGCGCGGGGTGATCGTC
AATACCGCCTCGGTGGCGGCGTTCGACGGGCAGATCGGCCAGGCCGCCTATTCCGCCTCGAAGAGCGGGGTG
GTCGGCATGACCCTGCCCATCGCCCGCGAGCTGGCGCGCTTCGGCATCCGAGTGATGACCATCGCCCCGGGC
ATCTTCGAGACACCGATGATGGCCGGCATGCCCCAGGAGGTGCGCGACTCCCTCGGCGCCAGCGTGCCGTTC
CCGCCGCGCCTGGGGCGTCCGGACGAATACGCCGCGCTGGTCCGGCAGATCATCGAGAACAGCATGCTCAAC
GGCGAAGTGATTCGCCTCGACGGCGCCATCCGCATGGCCGCCCGCTAA
Translation:
MRIENRVFLVTGGSSGLGAATAKMLVEQGGKVVLADINAEAGAAKAAELGAQARFVRADIASEADGRQAVAT
ALEAFGGLHGLANCAGVAPAEKVLGRNGIHALESFRRVIDINLVGSFNMLRLAAEAMSQGQPDEGGERGVIV
NTASVAAFDGQIGQAAYSASKSGVVGMTLPIARELARFGIRVMTIAPGIFETPMMAGMPQEVRDSLGASVPF
PPRLGRPDEYAALVRQIIENSMLNGEVIRLDGAIRMAAR*
AnnotationID:3292GeneID:3284
AnnotatorUID: diggins
Modification Date/Time: 2005-03-06 22:10:42
Gene Name:
Confidence Code:3
GeneProduct:putative short-chain dehydrogenase
Cell Localization:()
Synonyms:PA2554
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:putative 3-hydroxyacyl-CoA dehydrogenase
Functional Category Confidence Code:5
COGs:pfam00106,COG1028
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN33643.1|AE014543_7 (AE014543) 3-hydroxyacyl-CoA dehydrogenase, putative [Brucella suis
           1330]
          Length = 255

 Score =  380 bits (977), Expect = e-105
 Identities = 191/255 (74%), Positives = 216/255 (84%)

>gb|AAN67827.1|AE016414_2 (AE016782) 3-hydroxyacyl-CoA dehydrogenase FadB2x [Pseudomonas
           putida KT2440]
          Length = 255

 Score =  353 bits (905), Expect = 7e-97
 Identities = 179/255 (70%), Positives = 207/255 (81%)

>emb|CAD16241.1| (AL646070) PROBABLE 3-HYDROXYACYL-COA DEHYDROGENASE TYPE II
           OXIDOREDUCTASE PROTEIN [Ralstonia solanacearum]
          Length = 252

 Score =  332 bits (852), Expect = 1e-90
 Identities = 171/252 (67%), Positives = 202/252 (80%), Gaps = 3/252 (1%)






 
Structural Features:
pfam00106, adh_short, short chain dehydrogenase.
CD-Length = 247 residues,  99.6% aligned
Score =  159 bits (404), Expect = 3e-40
Identities = 96/256 (37%), Positives = 128/256 (50%), Gaps = 24/256 (9%)

COG1028, FabG, Dehydrogenases with different specificities 
(related to short-chain alcohol dehydrogenases) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 251 residues, 100.0% aligned
Score =  134 bits (338), Expect = 9e-33
Identities = 96/263 (36%), Positives = 135/263 (51%), Gaps = 24/263 (9%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14789




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23713




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33131




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3284_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3284
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5279
                           PA2554_tr
                           translation of PA2554
                           255
                           99.60
                           99.60
                           0
                           1248.0
     

     
     
148067
                           gb|AAG05942.1|AE004683_6
                           (AE004683) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           255
                           99.21
                           99.60
                           0
                           1245.0
     

     
     
148068
                           gb|AAN33643.1|AE014543_7
                           (AE014543) 3-hydroxyacyl-CoA dehydrogenase, putative [Brucella suis 1330]
                           255
                           74.50
                           84.70
                           0
                           973.0
     

     
     
148069
                           gb|AAL54058.1|
                           (AE009715) 3-OXOACYL-(ACYL-CARRIER PROTEIN) REDUCTASE [Brucella melitensis 16M]
                           255
                           74.50
                           84.70
                           0
                           972.0
     

     
     
148070
                           emb|CAE27744.1|
                           (BX572600) putative 3-hydroxyacyl-CoA dehydrogenase [Rhodopseudomonas palustris CGA009]
                           255
                           72.15
                           80.78
                           0
                           908.0
     

     






      

          
GC ORFID: 46286How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2554Glimmer Score: 

          
Start: 2213873Stop: 2213106

          
Length: 768


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2554 translation of PA2554Homolog Bit Score 485.0

          
Other Homologs:  t_PA2554 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGCGTATCGAAAATCGAGTGTTCCTGGTCACCGGCGGTAGCTCCGGGCTCGGCGCCGCCACCGCGAAGATG 
CTGGTGGAGCAGGGCGGCAAGGTGGTGCTGGCCGACATCAACGCCGAGGCCGGCGCGGCCAAGGCCGCCGAG 
CTTGGCGCCCAGGCGCGCTTCGTCAGGGCCGACATCGCCAGCGAGGCCGACGGCCGCCAGGCCGTGGCCACT 
GCACTGGAGGCCTTCGGCGGCCTCCATGGCCTGGCCAATTGCGCGGGCGTGGCGCCGGCCGAGAAGGTCCTC 
GGACGCAATGGCATCCATGCCCTGGAGAGTTTCCGCCGGGTCATCGACATCAACCTGGTGGGCAGCTTCAAC 
ATGCTGCGCCTGGCCGCCGAGGCCATGTCCCAGGGCCAGCCGGACGAGGGCGGCGAGCGCGGGGTGATCGTC 
AATACCGCCTCGGTGGCGGCGTTCGACGGGCAGATCGGCCAGGCCGCCTATTCCGCCTCGAAGAGCGGGGTG 
GTCGGCATGACCCTGCCCATCGCCCGCGAGCTGGCGCGCTTCGGCATCCGAGTGATGACCATCGCCCCGGGC 
ATCTTCGAGACACCGATGATGGCCGGCATGCCCCAGGAGGTGCGCGACTCCCTCGGCGCCAGCGTGCCGTTC 
CCGCCGCGCCTGGGGCGTCCGGACGAATACGCCGCGCTGGTCCGGCAGATCATCGAGAACAGCATGCTCAAC 
GGCGAAGTGATTCGCCTCGACGGCGCCATCCGCATGGCCGCCCGCTAA