ViewGene
Locus Name:
GeneID:
Location:

GeneID:3282
TrivialName: PA14_43180
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_43180
PAO1 Ortholog Locus:PA1649
Sequence Length:762
Protein Length:253
Start: 3847340
Stop: 3846579
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05038.1|AE004592_9 (AE004592) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 250/253 (98%), Positives = 251/253 (99%)
Sequence:
ATGAGCAAACTTCTTTCCGGCCAGGTCGCACTGGTCACTGGCGGTGCGGCGGGCATCGGCCGCGCTACCGCG
CTGGcctTCGCCGCCGCCGGGGTCAAGGTGGTGGTCGCCGACCTGGACAGCGCCGGCGGCGAGGGCACGGTC
GAAGCGATCCGCCAGGCGGGAGGCGAAGCCCTCTTCATTCGCTGCGACGTCACCCGCGACGCCGAGGTCAAG
GCGCTGGTAGAGGGTTGCGCGGCGGCCTACGGCCATCTCGACTACGCCTTCAACAACGCCGGCATCGAGATC
GAGCAGGGCAAGCTGGCCGACGGCAACGAAGCCGAGTTCGACGCCATCATGGCTGTCAACGTGAAGGGCGTC
TGGTTGTGCATGAAGCACCAGATCCCGCTGATGCTGGCCCAGGGCGGCGGCGCGATCGTCAACACCGCCTCG
GTCGCCGGGCTCGGCGCGGCGCCGAAGATGAGCATCTACGCTGCTTCCAAGCACGCGGTGATCGGCCTGACC
AAATCGGCGGCGATCGAGTACGCGAAGAAGGGCATCCGCGTCAACGCCGTGTGTCCGGCGGTGATCGACACC
GACATGTTCCGCCGCGCCTACGAGGCCGATCCGCGCAAGGCCGAGTTCGCCGCCGCGATGCATCCGCTGGGT
CGGGTCGGACGGGTCGAGGAAATCGCCGCCGCGGTGCTCTACCTGTGCAGCGACCACGCGGGCTTCACCACC
GGTATCGCCTTGCCAGTGGACGGCGGGGCGACGGCGATCTGA
Translation:
MSKLLSGQVALVTGGAAGIGRATALAFAAAGVKVVVADLDSAGGEGTVEAIRQAGGEALFIRCDVTRDAEVK
ALVEGCAAAYGHLDYAFNNAGIEIEQGKLADGNEAEFDAIMAVNVKGVWLCMKHQIPLMLAQGGGAIVNTAS
VAGLGAAPKMSIYAASKHAVIGLTKSAAIEYAKKGIRVNAVCPAVIDTDMFRRAYEADPRKAEFAAAMHPLG
RVGRVEEIAAAVLYLCSDHAGFTTGIALPVDGGATAI*
AnnotationID:3290GeneID:3282
AnnotatorUID: diggins
Modification Date/Time: 2005-02-27 16:04:01
Gene Name:
Confidence Code:3
GeneProduct:putative short-chain dehydrogenase
Cell Localization:()
Synonyms:PA1649
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00106,COG1028,COG4221
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67436.1|AE016371_2 (AE016780) oxidoreductase, short-chain dehydrogenase/reductase
           family [Pseudomonas putida KT2440]
          Length = 253

 Score =  410 bits (1054), Expect = e-114
 Identities = 211/253 (83%), Positives = 225/253 (88%)

>gb|AAM03863.1| (AE010702) 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase
           [Methanosarcina acetivorans str. C2A] [Methanosarcina
           acetivorans C2A]
          Length = 256

 Score =  240 bits (613), Expect = 5e-63
 Identities = 129/251 (51%), Positives = 164/251 (65%), Gaps = 1/251 (0%)

>gb|AAF84535.1|AE003996_4 (AE003996) 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase
           [Xylella fastidiosa 9a5c]
          Length = 255

 Score =  225 bits (574), Expect = 2e-58
 Identities = 120/243 (49%), Positives = 149/243 (61%)








 
Structural Features:
pfam00106, adh_short, short chain dehydrogenase.
CD-Length = 247 residues,  99.6% aligned
Score =  195 bits (496), Expect = 5e-51
Identities = 107/247 (43%), Positives = 139/247 (56%), Gaps = 8/247 (3%)

COG1028, FabG, Dehydrogenases with different specificities 
(related to short-chain alcohol dehydrogenases) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 251 residues,  99.2% aligned
Score =  171 bits (433), Expect = 8e-44
Identities = 117/252 (46%), Positives = 150/252 (59%), Gaps = 8/252 (3%)

COG4221, COG4221, Short-chain alcohol dehydrogenase of unknown specificity
CD-Length = 246 residues,  89.8% aligned
Score =  133 bits (337), Expect = 2e-32
Identities = 80/230 (34%), Positives = 118/230 (51%), Gaps = 13/230 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6567




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23709




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37289




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3282_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3282
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5274
                           PA1649_tr
                           translation of PA1649
                           253
                           98.81
                           99.20
                           0
                           1248.0
     

     
     
147905
                           gb|AAG05038.1|AE004592_9
                           (AE004592) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           253
                           98.81
                           99.20
                           0
                           1248.0
     

     
     
147906
                           gb|AAN67436.1|AE016371_2
                           (AE016780) oxidoreductase, short-chain dehydrogenase/reductase family [Pseudomonas putida KT2440]
                           253
                           83.39
                           88.93
                           0
                           1054.0
     

     
     
147907
                           dbj|BAC90779.1|
                           (AP006578) gll2838 [Gloeobacter violaceus PCC 7421]
                           253
                           51.77
                           69.16
                           4
                           651.0
     

     
     
147910
                           dbj|BAC75998.1|
                           (AB095015) short-chain dehydrogenase/redutase [Terrabacter sp. DBF63]
                           254
                           50.78
                           61.81
                           3
                           578.0
     

     






      

          
GC ORFID: 46284How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1649Glimmer Score: 

          
Start: 1106569Stop: 1107330

          
Length: 762


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1649 translation of PA1649Homolog Bit Score 485.0

          
Other Homologs:  t_PA4148 (ORF: BLASTX 178.0), t_PA1649 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAAACTTCTTTCCGGCCAGGTCGCACTGGTCACTGGCGGTGCGGCGGGCATCGGCCGCGCTACCGCG 
CTGGcctTCGCCGCCGCCGGGGTCAAGGTGGTGGTCGCCGACCTGGACAGCGCCGGCGGCGAGGGCACGGTC 
GAAGCGATCCGCCAGGCGGGAGGCGAAGCCCTCTTCATTCGCTGCGACGTCACCCGCGACGCCGAGGTCAAG 
GCGCTGGTAGAGGGTTGCGCGGCGGCCTACGGCCATCTCGACTACGCCTTCAACAACGCCGGCATCGAGATC 
GAGCAGGGCAAGCTGGCCGACGGCAACGAAGCCGAGTTCGACGCCATCATGGCTGTCAACGTGAAGGGCGTC 
TGGTTGTGCATGAAGCACCAGATCCCGCTGATGCTGGCCCAGGGCGGCGGCGCGATCGTCAACACCGCCTCG 
GTCGCCGGGCTCGGCGCGGCGCCGAAGATGAGCATCTACGCTGCTTCCAAGCACGCGGTGATCGGCCTGACC 
AAATCGGCGGCGATCGAGTACGCGAAGAAGGGCATCCGCGTCAACGCCGTGTGTCCGGCGGTGATCGACACC 
GACATGTTCCGCCGCGCCTACGAGGCCGATCCGCGCAAGGCCGAGTTCGCCGCCGCGATGCATCCGCTGGGT 
CGGGTCGGACGGGTCGAGGAAATCGCCGCCGCGGTGCTCTACCTGTGCAGCGACCACGCGGGCTTCACCACC 
GGTATCGCCTTGCCAGTGGACGGCGGGGCGACGGCGATCTGA