ViewGene
Locus Name:
GeneID:
Location:

GeneID:328
TrivialName: PA14_53340
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_53340
PAO1 Ortholog Locus:PA0845
Sequence Length:2013
Protein Length:670
Start: 4727802
Stop: 4729814
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: dbj|BAA88409.1| (AB028646) alkaline ceramidase [Pseudomonas aeruginosa]
Identities = 664/670 (99%), Positives = 668/670 (99%)
Sequence:
ATGTCACGTTCCGCATTCACCGCGCTCTTGCTGTCCTGCGTCCTGCTGGCGCTCTCCATGCCTGCCAGGGCC
GACGACCTGCCCTACCGCTTCGGCCTGGGCAAGGCGGACATCACCGGCGAAGCCGCCGAAGTCGGCATGATG
GGTTACTCCTCCCTCGAACAGAAGACCGCCGGCATCCACATGCGCCAGTGGGCGCGTGCCTTCGTGATCGAG
GAAGCGGCCAGCGGACGTCGCCTGGTCTACGTCAACACCGACCTGGGGATGATCTTCCAGGCCGTGCACCTG
AAGGTCCTGGCCCGGCTCAAGGCGAAGTACCCCGGTGTCTACGACGAGAACAACGTGATGCTCGCCGCCACC
CACACCCACTCCGGTCCGGGCGGCTTCTCCCACTACGCGATGTACAACCTGTCGGTGCTCGGCTTCCAGGAA
AAGACCTTCAATGCCATCGTCGACGGCATCGTCCGCTCCATCGAGCGGGCCCAGGCCAGGTTGCAGCCCGGC
CGCCTGTTCTACGGCAGCGGCGAGCTGCGCAACGCCAGCCGCAACCGTTCGCTGCTGTCGCACCTGAAGAAT
CCGGACATCGCCGGCTACGAGGATGGCATCGACCCGCAGATGAGCGTGCTCAGCTTCGTCGACGCCAACGGC
GAGCTGGCCGGCGCGATCAGTTGGTTCCCGGTGCACAGCACCTCGATGACCAACGCCAACCATCTGATCTCG
CCGGACAACAAGGGCTACGCCTCCTATCACTGGGAGCACGACGTCAGCCGCAGGGCCGGTTTCGTCGCCGCC
TTCGCCCAGACCAATGCCGGCAACCTGTCGCCCAACCTGAACCTGAAGCCCGGCTCCGGTCCCTTCGACAAC
GAGTTCGACAACACCCGCGAGATCGGTCTGCGCCAATTCGCCAAGGCCTACGAGATCGCCGGCCAGGCCCAG
GAGGAAGTGCTCGGCGAACTGGATTCGCGCTTCCGTTTCGTCGACTTCACCCGCCTGCCGATCCGCCCGGAG
TTCACCGACGGCCAGCCGCGCCAGTTGTGCACCGCGGCCATCGGCACCAGTCTGGCCGCCGGTAGCACCGAA
GACGGTCCAGGCCCGCTGGGGCTGGATGAAGGCAACAATCCGTTCCTCTCGGCCCTTGGCGGGTTGCTCACC
GGCGTGCCGCCGCAGGAACTGGTGCAATGCCAGGCGGAAAAGACCATCCTCGCCGACACCGGCAACAAGAAA
CCCTACCCCTGGACGCCGACGGTGCTGCCGATCCAGATGTTCCGCATCGGCCAGTTGGAACTGCTCGGCGCC
CCCGCCGAGTTCACCGTGATGGCCGGGGTGCGGATCCGCCGCGCGGTGCAGGCGGCCAGCGAAGCGGCCGGT
ATCCGCCATGTGATCTTCAATGGCTACGCGAATGCCTATGCCAGCTACGTCACCACCCGCGAGGAATACGCC
GCCCAGGAATACGAAGGCGGCTCGACCCTCTACGGCCCCTGGACCCAGGCCGCCTACCAGCAGTTGTTCGTC
GACATGGCGGTGGCGCTGCGCGAACGCCTGCCGGTGGAAACCTCGGCGATAGCGCCGGACCTGTCCTGCTGC
CAGATGAACTTCCAGACCGGAGTAGTGGCCGATGATCCCTATATCGGCAAGTCCTTCGGCGACGTGTTGCAA
CAACCCAGGGAAAGTTATCGCATCGGCGACAAGGTGACCGTCGCTTTCGTGACCGGACATCCGAAGAATGAC
TTGCGCACCGAGAAGACTTTCCTGGAAGTGGTGAATATCGGCAAGGATGGCAAACAGACGCCCGTGACCGTT
GCCACCGATAATGACTGGTATACCCAATACCGCTGGGAGAGAGTGGGTATATCTGCCTCGAAAGCGACTATC
AGCTGGTCCATTCCACCAGGGACCGAGCCCGGCCATTACTACATCAGGCACTATGGCAACGCGAAGAACTTC
TGGACCCAGAAGATCAGCGAAATCGGCGGCTCGACCCGCTCCTTCGAGGTGCTCGGCACCACTCCCTAG
Translation:
MSRSAFTALLLSCVLLALSMPARADDLPYRFGLGKADITGEAAEVGMMGYSSLEQKTAGIHMRQWARAFVIE
EAASGRRLVYVNTDLGMIFQAVHLKVLARLKAKYPGVYDENNVMLAATHTHSGPGGFSHYAMYNLSVLGFQE
KTFNAIVDGIVRSIERAQARLQPGRLFYGSGELRNASRNRSLLSHLKNPDIAGYEDGIDPQMSVLSFVDANG
ELAGAISWFPVHSTSMTNANHLISPDNKGYASYHWEHDVSRRAGFVAAFAQTNAGNLSPNLNLKPGSGPFDN
EFDNTREIGLRQFAKAYEIAGQAQEEVLGELDSRFRFVDFTRLPIRPEFTDGQPRQLCTAAIGTSLAAGSTE
DGPGPLGLDEGNNPFLSALGGLLTGVPPQELVQCQAEKTILADTGNKKPYPWTPTVLPIQMFRIGQLELLGA
PAEFTVMAGVRIRRAVQAASEAAGIRHVIFNGYANAYASYVTTREEYAAQEYEGGSTLYGPWTQAAYQQLFV
DMAVALRERLPVETSAIAPDLSCCQMNFQTGVVADDPYIGKSFGDVLQQPRESYRIGDKVTVAFVTGHPKND
LRTEKTFLEVVNIGKDGKQTPVTVATDNDWYTQYRWERVGISASKATISWSIPPGTEPGHYYIRHYGNAKNF
WTQKISEIGGSTRSFEVLGTTP*
AnnotationID:338GeneID:328
AnnotatorUID: danlee
Modification Date/Time: 2004-04-30 16:04:28
Gene Name:
Confidence Code:3
GeneProduct:alkaline ceramidase
Cell Localization:()
Synonyms:PA0845
Cell Localization Confidence Code:5
MolecularFunction:Catalyzes the hydrolysis of the N-acyl linkage of ceramide to generate free fatty acid and sphingosine base.
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam04734, KOG2232
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
99% identical to alkaline ceramidase from Pseudomonas aeruginosa strain AN17.






 
Structural Features:
pfam04734, Ceramidase_alk, Neutral/alkaline non-lysosomal ceramidase. Score: 925, 0.0
KOG2232, Ceramidases [Signal transduction mechanisms].  Score: 522, 1e-148






   
Genomic Context:






   
Comment:


















    ReferenceID:659




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17801




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31979




  
Author/Investigator(s):
        Okino N, Ichinose S, Omori A, Imayama S, Nakamura T, Ito M.






  
Title:
        Molecular cloning, sequencing, and expression of the gene encoding alkaline ceramidase from Pseudomonas aeruginosa. Cloning of a ceramidase homologue from Mycobacterium tuberculosis.




  
PubMed:
        10593963


MedLine:
        20062886




    
Source:
J Biol Chem. 1999 Dec 17;274(51):36616-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:328
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
26878
                           dbj|BAA88409.1|
                           (AB028646) alkaline ceramidase [Pseudomonas aeruginosa]
                           670
                           99.10
                           99.70
                           0
                           3475.0
     

     
     
544
                           PA0845_tr
                           translation of PA0845
                           670
                           98.80
                           99.55
                           0
                           3467.0
     

     
     
26879
                           gb|AAG04234.1|AE004519_5
                           (AE004519) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           670
                           98.80
                           99.55
                           0
                           3467.0
     

     
     
26880
                           emb|CAC67511.1|
                           (AJ315932) ceramide-hydrolysing enzyme [Pseudomonas aeruginosa]
                           670
                           98.80
                           99.55
                           0
                           3467.0
     

     
     
26881
                           emb|CAB09388.1|
                           (BX842574) POSSIBLE HYDROLASE [Mycobacterium tuberculosis H37Rv]
                           686
                           39.06
                           54.81
                           65
                           1142.0
     

     






      

          
GC ORFID: 43330How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0845Glimmer Score: 

          
Start: 226105Stop: 224093

          
Length: 2013


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA0845 translation of PA0845Homolog Bit Score 1340.0

          
Other Homologs:  t_PA0845 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCACGTTCCGCATTCACCGCGCTCTTGCTGTCCTGCGTCCTGCTGGCGCTCTCCATGCCTGCCAGGGCC 
GACGACCTGCCCTACCGCTTCGGCCTGGGCAAGGCGGACATCACCGGCGAAGCCGCCGAAGTCGGCATGATG 
GGTTACTCCTCCCTCGAACAGAAGACCGCCGGCATCCACATGCGCCAGTGGGCGCGTGCCTTCGTGATCGAG 
GAAGCGGCCAGCGGACGTCGCCTGGTCTACGTCAACACCGACCTGGGGATGATCTTCCAGGCCGTGCACCTG 
AAGGTCCTGGCCCGGCTCAAGGCGAAGTACCCCGGTGTCTACGACGAGAACAACGTGATGCTCGCCGCCACC 
CACACCCACTCCGGTCCGGGCGGCTTCTCCCACTACGCGATGTACAACCTGTCGGTGCTCGGCTTCCAGGAA 
AAGACCTTCAATGCCATCGTCGACGGCATCGTCCGCTCCATCGAGCGGGCCCAGGCCAGGTTGCAGCCCGGC 
CGCCTGTTCTACGGCAGCGGCGAGCTGCGCAACGCCAGCCGCAACCGTTCGCTGCTGTCGCACCTGAAGAAT 
CCGGACATCGCCGGCTACGAGGATGGCATCGACCCGCAGATGAGCGTGCTCAGCTTCGTCGACGCCAACGGC 
GAGCTGGCCGGCGCGATCAGTTGGTTCCCGGTGCACAGCACCTCGATGACCAACGCCAACCATCTGATCTCG 
CCGGACAACAAGGGCTACGCCTCCTATCACTGGGAGCACGACGTCAGCCGCAGGGCCGGTTTCGTCGCCGCC 
TTCGCCCAGACCAATGCCGGCAACCTGTCGCCCAACCTGAACCTGAAGCCCGGCTCCGGTCCCTTCGACAAC 
GAGTTCGACAACACCCGCGAGATCGGTCTGCGCCAATTCGCCAAGGCCTACGAGATCGCCGGCCAGGCCCAG 
GAGGAAGTGCTCGGCGAACTGGATTCGCGCTTCCGTTTCGTCGACTTCACCCGCCTGCCGATCCGCCCGGAG 
TTCACCGACGGCCAGCCGCGCCAGTTGTGCACCGCGGCCATCGGCACCAGTCTGGCCGCCGGTAGCACCGAA 
GACGGTCCAGGCCCGCTGGGGCTGGATGAAGGCAACAATCCGTTCCTCTCGGCCCTTGGCGGGTTGCTCACC 
GGCGTGCCGCCGCAGGAACTGGTGCAATGCCAGGCGGAAAAGACCATCCTCGCCGACACCGGCAACAAGAAA 
CCCTACCCCTGGACGCCGACGGTGCTGCCGATCCAGATGTTCCGCATCGGCCAGTTGGAACTGCTCGGCGCC 
CCCGCCGAGTTCACCGTGATGGCCGGGGTGCGGATCCGCCGCGCGGTGCAGGCGGCCAGCGAAGCGGCCGGT 
ATCCGCCATGTGATCTTCAATGGCTACGCGAATGCCTATGCCAGCTACGTCACCACCCGCGAGGAATACGCC 
GCCCAGGAATACGAAGGCGGCTCGACCCTCTACGGCCCCTGGACCCAGGCCGCCTACCAGCAGTTGTTCGTC 
GACATGGCGGTGGCGCTGCGCGAACGCCTGCCGGTGGAAACCTCGGCGATAGCGCCGGACCTGTCCTGCTGC 
CAGATGAACTTCCAGACCGGAGTAGTGGCCGATGATCCCTATATCGGCAAGTCCTTCGGCGACGTGTTGCAA 
CAACCCAGGGAAAGTTATCGCATCGGCGACAAGGTGACCGTCGCTTTCGTGACCGGACATCCGAAGAATGAC 
TTGCGCACCGAGAAGACTTTCCTGGAAGTGGTGAATATCGGCAAGGATGGCAAACAGACGCCCGTGACCGTT 
GCCACCGATAATGACTGGTATACCCAATACCGCTGGGAGAGAGTGGGTATATCTGCCTCGAAAGCGACTATC 
AGCTGGTCCATTCCACCAGGGACCGAGCCCGGCCATTACTACATCAGGCACTATGGCAACGCGAAGAACTTC 
TGGACCCAGAAGATCAGCGAAATCGGCGGCTCGACCCGCTCCTTCGAGGTGCTCGGCACCACTCCCTAG