ViewGene
Locus Name:
GeneID:
Location:

GeneID:3276
TrivialName: PA14_62830
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62830
PAO1 Ortholog Locus:PA4748
Sequence Length:756
Protein Length:251
Start: 5605430
Stop: 5604675
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08134.1|AE004888_9 (AE004888) triosephosphate isomerase [Pseudomonas aeruginosa PAO1]
Identities = 251/251 (100%), Positives = 251/251 (100%)
Sequence:
ATGCGTCGACCCTTGGTGGCCGGTAACTGGAAAATGCACGGTACGCATTCCAGTGTGGCCGAGTTGATCAAA
GGCTTGCGTCAGCTGGCGTTGCCGAGCGGAGTCGATGTAGCTGTGATGCCGCCTTGCTTGTTCATCAGCCAG
GTCATCCAGGGCCTGGCCGGCAAGGCGATCGATGTAGGTGCGCAGAACAGCGCCGTCGAGCCGATGCAAGGC
GCGCTGACCGGTGAGACTGCTCCCAGTCAGTTGGCGGATGTCGGTTGTAGCATGGTCCTTGTGGGCCACTCG
GAGCGTCGCCTGATTCTCGGCGAGAGTGACGAGGTTGTGAGTCGCAAGTTTGCCGCGGCTCAGTCGTGCGGC
CTGGTGCCGGTGCTGTGTGTCGGGGAAACCCGGGCGGAGCGCGAGGCGGGCAAGACGCTGGAGGTTGTCGCA
AGGCAGCTGGGAAGCGTGATCGACGAGTTGGGTGTTGGAGCTTTTGCTCGCGCAGTCGTGGCTTACGAGCCG
GTCTGGGCGATCGGGACCGGGTTGACCGCGTCCCCCGCGCAAGCCCAGGAAGTGCACGCGGCGATCCGCGCG
CAACTGGCAGCGGAAAATGCCGAGGTCGCAAAAGGTGTGCGACTCCTTTACGGCGGCAGTGTAAAGGCGGCA
AGTGCAGCCGAGTTGTTCGGCATGCCGGATATCGATGGGGGGCTGGTAGGTGGAGCCTCCCTCAATGCGGAT
GAGTTCGGCGCGATCTGTCGTGCCGCGGGAAGCTGA
Translation:
MRRPLVAGNWKMHGTHSSVAELIKGLRQLALPSGVDVAVMPPCLFISQVIQGLAGKAIDVGAQNSAVEPMQG
ALTGETAPSQLADVGCSMVLVGHSERRLILGESDEVVSRKFAAAQSCGLVPVLCVGETRAEREAGKTLEVVA
RQLGSVIDELGVGAFARAVVAYEPVWAIGTGLTASPAQAQEVHAAIRAQLAAENAEVAKGVRLLYGGSVKAA
SAAELFGMPDIDGGLVGGASLNADEFGAICRAAGS*
AnnotationID:3284GeneID:3276
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 07:07:34
Gene Name:tpiA
Confidence Code:2
GeneProduct:triosephosphate isomerase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00121,COG0149
Secondary Functional Category(ies):energy production and conversion
EC Number:5.3.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70287.1|AE016669_5 (AE016791) triosephosphate isomerase [Pseudomonas putida KT2440]
          Length = 251

 Score =  392 bits (1007), Expect = e-108
 Identities = 197/251 (78%), Positives = 221/251 (88%)

>gb|AAO57942.1| (AE016872) triosephosphate isomerase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 251

 Score =  374 bits (959), Expect = e-103
 Identities = 187/251 (74%), Positives = 216/251 (86%)

>gb|AAN83299.1|AE016770_99 (AE016770) Triosephosphate isomerase [Escherichia coli CFT073]
          Length = 255

 Score =  250 bits (639), Expect = 5e-66
 Identities = 132/250 (52%), Positives = 171/250 (68%), Gaps = 2/250 (0%)






 
Structural Features:
pfam00121, TIM, Triosephosphate isomerase.
CD-Length = 249 residues,  99.6% aligned
Score =  290 bits (744), Expect = 9e-80
Identities = 133/249 (53%), Positives = 167/249 (67%), Gaps = 2/249 (0%)

COG0149, TpiA, Triosephosphate isomerase 
[Carbohydrate transport and metabolism]
CD-Length = 251 residues,  98.4% aligned
Score =  273 bits (699), Expect = 1e-74
Identities = 133/251 (52%), Positives = 164/251 (65%), Gaps = 6/251 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6555




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23697




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37666




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3276_cdd.html


    		




 
URL:

















    ReferenceID:37724




  
Author/Investigator(s):
        Tavares IM, Leitao JH, Sa-Correia I.





  
Title:
        Chromosomal organization and transcription analysis of genes in the vicinity of Pseudomonas aeruginosa glmM gene encoding phosphoglucosamine mutase.




  
PubMed:
        12604356


MedLine:
        




    
Source:
Biochem Biophys Res Commun. 2003 Mar 7;302(2):363-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12604356









           
Homologs By Global Alignment 

           

       



     
Gene ID:3276
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5257
                           PA4748_tr
                           translation of PA4748
                           251
                           99.99
                           99.99
                           0
                           1250.0
     

     
     
146847
                           gb|AAG08134.1|AE004888_9
                           (AE004888) triosephosphate isomerase [Pseudomonas aeruginosa PAO1]
                           251
                           99.99
                           99.99
                           0
                           1250.0
     

     
     
146848
                           gb|AAN70287.1|AE016669_5
                           (AE016791) triosephosphate isomerase [Pseudomonas putida KT2440]
                           251
                           78.48
                           88.04
                           0
                           1007.0
     

     
     
146849
                           gb|AAO57942.1|
                           (AE016872) triosephosphate isomerase [Pseudomonas syringae pv. tomato str. DC3000]
                           251
                           74.50
                           86.05
                           0
                           959.0
     

     
     
146850
                           gb|AAC45131.1|
                           (U85643) triose phosphate isomerase [Pseudomonas syringae pv. syringae]
                           251
                           73.30
                           84.46
                           0
                           938.0
     

     






      

          
GC ORFID: 46278How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4748Glimmer Score: 

          
Start: 4869110Stop: 4868355

          
Length: 756


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4748 translation of PA4748Homolog Bit Score 486.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5488 (ORF: BLASTX 365.0), t_PA4748 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGTCGACCCTTGGTGGCCGGTAACTGGAAAATGCACGGTACGCATTCCAGTGTGGCCGAGTTGATCAAA 
GGCTTGCGTCAGCTGGCGTTGCCGAGCGGAGTCGATGTAGCTGTGATGCCGCCTTGCTTGTTCATCAGCCAG 
GTCATCCAGGGCCTGGCCGGCAAGGCGATCGATGTAGGTGCGCAGAACAGCGCCGTCGAGCCGATGCAAGGC 
GCGCTGACCGGTGAGACTGCTCCCAGTCAGTTGGCGGATGTCGGTTGTAGCATGGTCCTTGTGGGCCACTCG 
GAGCGTCGCCTGATTCTCGGCGAGAGTGACGAGGTTGTGAGTCGCAAGTTTGCCGCGGCTCAGTCGTGCGGC 
CTGGTGCCGGTGCTGTGTGTCGGGGAAACCCGGGCGGAGCGCGAGGCGGGCAAGACGCTGGAGGTTGTCGCA 
AGGCAGCTGGGAAGCGTGATCGACGAGTTGGGTGTTGGAGCTTTTGCTCGCGCAGTCGTGGCTTACGAGCCG 
GTCTGGGCGATCGGGACCGGGTTGACCGCGTCCCCCGCGCAAGCCCAGGAAGTGCACGCGGCGATCCGCGCG 
CAACTGGCAGCGGAAAATGCCGAGGTCGCAAAAGGTGTGCGACTCCTTTACGGCGGCAGTGTAAAGGCGGCA 
AGTGCAGCCGAGTTGTTCGGCATGCCGGATATCGATGGGGGGCTGGTAGGTGGAGCCTCCCTCAATGCGGAT 
GAGTTCGGCGCGATCTGTCGTGCCGCGGGAAGCTGA