ViewGene
Locus Name:
GeneID:
Location:

GeneID:3275
TrivialName: PA14_11090
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_11090
PAO1 Ortholog Locus:PA4083
Sequence Length:741
Protein Length:246
Start: 961011
Stop: 961751
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07470.1|AE004824_5 (AE004824) chaperone CupB4 [Pseudomonas aeruginosa PAO1]
Identities = 243/246 (98%), Positives = 245/246 (99%)
Sequence:
GTGACCGGCGTCGGCCTGGGACGATGCGGCACGGCCTGGGTCCTGCTGGTGGCGCTGGTGGCCGATGCCTCC
GCCTCGCTGTCCGTGATCGGGACCCGTTTCATCTATCCGGCGGGCGTTTCCGCGCTGACGATTCGCGTCGGT
AACGTCGGGACGCGCCCGGCCCTGGTCCAGGCCTGGCTCGATCGCGGCGACGAGACGGCGGACCCGAGCGCT
GTCACGGTGCCGTTCATCCTGTCGCCGCCGCTGTTGCGCATGGACCCGCAGGAGACCCAGGCGCTCCAGCTT
CGCCATACCGGCGAGCCGCTGCCGGACGACCGGGAATCGCTGTTCTGGGTCAACCTGCTGGAGGTGCCGGGG
CGGGAGGACGGCAGCGGCAACCTGCTGCTGGTGAGCTACCGCCTGCGGATGAAGCTCCTGTTCCGTCCGCAG
GGTCTGGCCGGCGATCCGCGAGCCGCCGCGCGCCAGGTCGTCTGGCGCCTGCGTCCGGCGGTACGGCCGGGG
CAGCGGGCGCTGCTGGAGGCGGACAACCGCAGTCCCTATCACGTTTCCCTGGTGCGCCTGGAGCTGGGCGAG
GGCGACCTCGCCATGTCCCTCGGCAGCCTCACCCTGCCGCCTTTCGCCCTTACCCCGTTGAGTCTGCCGGCC
GTGCCCGGCGAGGCGGCGCGGGTCCATTTCGATGCGGTGGGGGATGACGGCCAGATCGAGCGCGGCGACGCG
CCGGCAGAGAGATACCCGTAG
Translation:
MTGVGLGRCGTAWVLLVALVADASASLSVIGTRFIYPAGVSALTIRVGNVGTRPALVQAWLDRGDETADPSA
VTVPFILSPPLLRMDPQETQALQLRHTGEPLPDDRESLFWVNLLEVPGREDGSGNLLLVSYRLRMKLLFRPQ
GLAGDPRAAARQVVWRLRPAVRPGQRALLEADNRSPYHVSLVRLELGEGDLAMSLGSLTLPPFALTPLSLPA
VPGEAARVHFDAVGDDGQIERGDAPAERYP*
AnnotationID:3283GeneID:3275
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 08:08:04
Gene Name:cupB4
Confidence Code:2
GeneProduct:chaperone CupB4
Cell Localization:()
Synonyms:fimB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3121,pfam00345
Secondary Functional Category(ies):chaperones and heat shock proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAG54444.1|AE005190_6 (AE005190) putative fimbrial chaperone protein [Escherichia coli
           O157:H7 EDL933]
          Length = 243

 Score =  157 bits (398), Expect = 4e-38
 Identities = 83/219 (37%), Positives = 130/219 (59%), Gaps = 13/219 (5%)

>emb|CAA47265.1| (X66729) filamentous hemagglutinin [Bordetella pertussis]
          Length = 244

 Score =  156 bits (394), Expect = 1e-37
 Identities = 98/240 (40%), Positives = 142/240 (59%), Gaps = 22/240 (9%)

>emb|CAE33483.1| (BX640446) chaperone protein [Bordetella bronchiseptica]
          Length = 274

 Score =  152 bits (384), Expect = 2e-36
 Identities = 97/240 (40%), Positives = 140/240 (58%), Gaps = 22/240 (9%)






 
Structural Features:
COG3121, FimC, P pilus assembly protein, chaperone PapD 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 235 residues,  96.2% aligned
Score =  175 bits (444), Expect = 6e-45
Identities = 86/237 (36%), Positives = 131/237 (55%), Gaps = 13/237 (5%)

pfam00345, Pili_assembly_N, Gram-negative pili assembly chaperone, N-terminal domain. 
CD-Length = 122 residues, 100.0% aligned
Score =  118 bits (298), Expect = 5e-28
Identities = 50/126 (39%), Positives = 74/126 (58%), Gaps = 8/126 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14787




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23695




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36830




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3275_cdd.html


    		




 
URL:

















    ReferenceID:36937




  
Author/Investigator(s):
        Vallet I, Olson JW, Lory S, Lazdunski A, Filloux A.





  
Title:
        The chaperone/usher pathways of Pseudomonas aeruginosa: identification of fimbrial gene clusters (cup) and their involvement in biofilm formation.




  
PubMed:
        11381121


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6911-6. Epub 2001 May 29.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=11381121

















    ReferenceID:36939




  
Author/Investigator(s):
        Klemm P, Jorgensen BJ, van Die I, de Ree H, Bergmans H.





  
Title:
        The fim genes responsible for synthesis of type 1 fimbriae in Escherichia coli, cloning and genetic organization.




  
PubMed:
        2863734


MedLine:
        




    
Source:
Mol Gen Genet. 1985;199(3):410-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2863734









           
Homologs By Global Alignment 

           

       



     
Gene ID:3275
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5256
                           PA4083_tr
                           translation of PA4083
                           246
                           98.78
                           99.59
                           0
                           1251.0
     

     
     
146841
                           gb|AAG07470.1|AE004824_5
                           (AE004824) chaperone CupB4 [Pseudomonas aeruginosa PAO1]
                           246
                           98.37
                           99.59
                           0
                           1248.0
     

     
     
146842
                           emb|CAE42164.1|
                           (BX640416) chaperone protein [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           265
                           38.11
                           55.09
                           40
                           406.0
     

     
     
146843
                           emb|CAA47265.1|
                           (X66729) filamentous hemagglutinin [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           265
                           38.11
                           55.09
                           40
                           406.0
     

     
     
146844
                           emb|CAA46089.1|
                           (X64876) FimB chaperone-like protein precursor [Bordetella pertussis]
                           265
                           37.73
                           54.71
                           40
                           400.0
     

     






      

          
GC ORFID: 46277How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4083Glimmer Score: 

          
Start: 3992926Stop: 3992186

          
Length: 741


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4083 translation of PA4083Homolog Bit Score 486.0

          
Other Homologs:  t_PA4083 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGACCGGCGTCGGCCTGGGACGATGCGGCACGGCCTGGGTCCTGCTGGTGGCGCTGGTGGCCGATGCCTCC 
GCCTCGCTGTCCGTGATCGGGACCCGTTTCATCTATCCGGCGGGCGTTTCCGCGCTGACGATTCGCGTCGGT 
AACGTCGGGACGCGCCCGGCCCTGGTCCAGGCCTGGCTCGATCGCGGCGACGAGACGGCGGACCCGAGCGCT 
GTCACGGTGCCGTTCATCCTGTCGCCGCCGCTGTTGCGCATGGACCCGCAGGAGACCCAGGCGCTCCAGCTT 
CGCCATACCGGCGAGCCGCTGCCGGACGACCGGGAATCGCTGTTCTGGGTCAACCTGCTGGAGGTGCCGGGG 
CGGGAGGACGGCAGCGGCAACCTGCTGCTGGTGAGCTACCGCCTGCGGATGAAGCTCCTGTTCCGTCCGCAG 
GGTCTGGCCGGCGATCCGCGAGCCGCCGCGCGCCAGGTCGTCTGGCGCCTGCGTCCGGCGGTACGGCCGGGG 
CAGCGGGCGCTGCTGGAGGCGGACAACCGCAGTCCCTATCACGTTTCCCTGGTGCGCCTGGAGCTGGGCGAG 
GGCGACCTCGCCATGTCCCTCGGCAGCCTCACCCTGCCGCCTTTCGCCCTTACCCCGTTGAGTCTGCCGGCC 
GTGCCCGGCGAGGCGGCGCGGGTCCATTTCGATGCGGTGGGGGATGACGGCCAGATCGAGCGCGGCGACGCG 
CCGGCAGAGAGATACCCGTAG