ViewGene
Locus Name:
GeneID:
Location:

GeneID:3265
TrivialName: PA14_13810
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_13810
PAO1 Ortholog Locus:PA3873
Sequence Length:741
Protein Length:246
Start: 1188951
Stop: 1189691
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07260.1|AE004804_8 (AE004804) respiratory nitrate reductase delta chain [Pseudomonas aeruginosa PAO1]
Identities = 246/246 (100%), Positives = 246/246 (100%)
Sequence:
ATGAACGATCACAGCCAACTGTTCCGCCTGCTCGCCCTGCTGCTCGACTATCCACGCGCCGAGCTGCGCGAG
GAGAGCCTCGGCCTGCATGCGCTGATCCGCACCTGCGAACTGCCGGAAGCGCTGCGCGACGGCCTCGCGGCG
CTGCTCAACGAGCTCTGCCAGGGCGACCTGCTGGACGTCCAGGCGCGCTACGACGGTCTCTTCGAGCGCGGC
CGCTCGGTCTCGCTGCTGCTCTTCGAGCACGTCCACGGCGAGAGCCGCGACCGTGGCCAGGCGATGGTCGAC
CTGCTCGACCGCTATACCGGGGCCGGCCTGCAGATCGACGTACCGGAGCTGCCGGACTACCTGCCGCTGTAC
CTCGAATACCTGTCGCTGCTGCCGTTCGCGGCGGCCAGCGAAGGGCTCGCCGAAGTGGCGCACATCCTCGGC
CTGCTGGCGCTGCGCCTGGAGGAACGCGGCAGCGCCTACGCGGCGATTTTCGAGGCGTTGCTGGAACTCGGC
GGCGAGCGCCCGGACCTCGGCGCGTTGCGTCGCGACCAGGCCCAGGAACAGCGCGACGACAGCCTGGAGGCC
ATCGACCGGGCCTGGGAGGAAACCCCGGTGAGCTTCACCGACCCTGCCGGCGGTTGCCCGTCGAGCAGCGGC
CGCCGTCCGACGGCGTCCACCGAACAACCATTGCAATGGGTCGCCCAGCCGGTACCGCAGATGCAGTACCGC
GCGGCCCGCGAAGGAGTCTGA
Translation:
MNDHSQLFRLLALLLDYPRAELREESLGLHALIRTCELPEALRDGLAALLNELCQGDLLDVQARYDGLFERG
RSVSLLLFEHVHGESRDRGQAMVDLLDRYTGAGLQIDVPELPDYLPLYLEYLSLLPFAAASEGLAEVAHILG
LLALRLEERGSAYAAIFEALLELGGERPDLGALRRDQAQEQRDDSLEAIDRAWEETPVSFTDPAGGCPSSSG
RRPTASTEQPLQWVAQPVPQMQYRAAREGV*
AnnotationID:3273GeneID:3265
AnnotatorUID: diggins
Modification Date/Time: 2005-04-25 09:09:03
Gene Name:narJ
Confidence Code:2
GeneProduct:respiratory nitrate reductase delta chain
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2180,pfam02613
Secondary Functional Category(ies):
EC Number:1.7.99.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAG34375.1|AF197465_7 (AF197465) putative chaperone protein; NarJ [Pseudomonas
           fluorescens]
          Length = 237

 Score =  201 bits (510), Expect = 4e-51
 Identities = 117/235 (49%), Positives = 147/235 (62%), Gaps = 13/235 (5%)

>gb|AAO69296.1| (AE016839) respiratory nitrate reductase 1 delta chain [Salmonella
           enterica subsp. enterica serovar Typhi Ty2]
          Length = 236

 Score =  174 bits (442), Expect = 3e-43
 Identities = 96/196 (48%), Positives = 127/196 (64%), Gaps = 1/196 (0%)






 
Structural Features:
COG2180, NarJ, Nitrate reductase delta subunit 
[Energy production and conversion]
CD-Length = 179 residues, 100.0% aligned
Score =  176 bits (448), Expect = 4e-45
Identities = 89/182 (48%), Positives = 111/182 (60%), Gaps = 3/182 (1%)

pfam02613, Nitrate_red_del, Nitrate reductase delta subunit.
CD-Length = 159 residues,  99.4% aligned
Score =  122 bits (306), Expect = 1e-28
Identities = 77/162 (47%), Positives = 92/162 (56%), Gaps = 4/162 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6533




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23675




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31691




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3265_cdd.html


    		




 
URL:

















    ReferenceID:34328




  
Author/Investigator(s):
        Berks BC, Page MD, Richardson DJ, Reilly A, Cavill A, Outen F, Ferguson SJ.





  
Title:
        Sequence analysis of subunits of the membrane-bound nitrate reductase from a denitrifying bacterium: the integral membrane subunit provides a prototype for the dihaem electron-carrying arm of a redox loop.




  
PubMed:
        7746153


MedLine:
        




    
Source:
Mol Microbiol. 1995 Jan;15(2):319-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7746153

















    ReferenceID:34329




  
Author/Investigator(s):
        Blasco F, Iobbi C, Giordano G, Chippaux M, Bonnefoy V.





  
Title:
        Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer.




  
PubMed:
        2674654


MedLine:
        




    
Source:
Mol Gen Genet. 1989 Aug;218(2):249-56.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2674654

















    ReferenceID:34330




  
Author/Investigator(s):
        Sodergren EJ, Hsu PY, DeMoss JA.





  
Title:
        Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli.




  
PubMed:
        3053688


MedLine:
        




    
Source:
J Biol Chem. 1988 Nov 5;263(31):16156-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3053688









           
Homologs By Global Alignment 

           

       



     
Gene ID:3265
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5243
                           PA3873_tr
                           translation of PA3873
                           246
                           99.99
                           99.99
                           0
                           1255.0
     

     
     
146594
                           gb|AAG07260.1|AE004804_8
                           (AE004804) respiratory nitrate reductase delta chain [Pseudomonas aeruginosa PAO1]
                           246
                           99.99
                           99.99
                           0
                           1255.0
     

     
     
146595
                           emb|CAA75542.1|
                           (Y15252) putative chaperone [Pseudomonas aeruginosa]
                           246
                           99.99
                           99.99
                           0
                           1255.0
     

     
     
146596
                           gb|AAG34375.1|AF197465_7
                           (AF197465) putative chaperone protein; NarJ [Pseudomonas fluorescens]
                           260
                           45.00
                           56.53
                           37
                           519.0
     

     
     
146607
                           gb|AAL20677.1|
                           (AE008778) nitrate reductase 1, delta subunit [Salmonella typhimurium LT2]
                           253
                           42.29
                           54.15
                           24
                           429.0
     

     






      

          
GC ORFID: 46267How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3873Glimmer Score: 

          
Start: 3764987Stop: 3764247

          
Length: 741


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3873 translation of PA3873Homolog Bit Score 488.0

          
Other Homologs:  t_PA3873 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGATCACAGCCAACTGTTCCGCCTGCTCGCCCTGCTGCTCGACTATCCACGCGCCGAGCTGCGCGAG 
GAGAGCCTCGGCCTGCATGCGCTGATCCGCACCTGCGAACTGCCGGAAGCGCTGCGCGACGGCCTCGCGGCG 
CTGCTCAACGAGCTCTGCCAGGGCGACCTGCTGGACGTCCAGGCGCGCTACGACGGTCTCTTCGAGCGCGGC 
CGCTCGGTCTCGCTGCTGCTCTTCGAGCACGTCCACGGCGAGAGCCGCGACCGTGGCCAGGCGATGGTCGAC 
CTGCTCGACCGCTATACCGGGGCCGGCCTGCAGATCGACGTACCGGAGCTGCCGGACTACCTGCCGCTGTAC 
CTCGAATACCTGTCGCTGCTGCCGTTCGCGGCGGCCAGCGAAGGGCTCGCCGAAGTGGCGCACATCCTCGGC 
CTGCTGGCGCTGCGCCTGGAGGAACGCGGCAGCGCCTACGCGGCGATTTTCGAGGCGTTGCTGGAACTCGGC 
GGCGAGCGCCCGGACCTCGGCGCGTTGCGTCGCGACCAGGCCCAGGAACAGCGCGACGACAGCCTGGAGGCC 
ATCGACCGGGCCTGGGAGGAAACCCCGGTGAGCTTCACCGACCCTGCCGGCGGTTGCCCGTCGAGCAGCGGC 
CGCCGTCCGACGGCGTCCACCGAACAACCATTGCAATGGGTCGCCCAGCCGGTACCGCAGATGCAGTACCGC 
GCGGCCCGCGAAGGAGTCTGA