ViewGene
Locus Name:
GeneID:
Location:

GeneID:3262
TrivialName: PA14_44020
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_44020
PAO1 Ortholog Locus:PA1584
Sequence Length:708
Protein Length:235
Start: 3920229
Stop: 3919522
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04973.1|AE004586_11 (AE004586) succinate dehydrogenase (B subunit) [Pseudomonas aeruginosa PAO1]
Identities = 235/235 (100%), Positives = 235/235 (100%)
Sequence:
ATGTTGCAAGTGAGTGTTTATCGTTACAACCCCGACAAAGATGCTGCGCCGTACATGCAGGATTTCCAGGTC
GACACCAACGGCAAGGACGTGATGGTCCTCGACGTGCTGGCGCTGATCAAGGAACAGGACGAGGGCTTCTCC
TACCGTCGTTCCTGCCGTGAAGGCGTCTGCGGCTCCGATGGCATGAACATCAACGGCAAGAACGGCCTGGCC
TGCATCACCCCGCTGTCGGCCGCCGGCCTGAAGGGCGGCAAGCTGGTGATCCGTCCGCTGCCCGGCCTGCCG
GTCATTCGTGACCTGGTCGTGGACATGAGCATCTTCTACAAGCAATACGAGAAGGTGAAGCCGTTCCTGCAG
AACGATACGCCGGCCCCGGCCATTGAGCGTCTGCAGACCCCGGAAGAGCGCGAGAAGCTGGACGGTCTGTAC
GAGTGCATCCTGTGCGCCTGCTGCTCGACCTCCTGCCCGTCCTTCTGGTGGAACCCCGACAAGTTCCTCGGT
CCGGCCGCACTGCTGCAAGCCTATCGTTTCCTGGCCGACAGCCGTGACACCAAGACCGAAGAGCGCCTGGCT
TCGCTGGACGACCCGTTCAGTGTGTTCCGCTGCCGCGGAATCATGAACTGCGTGAACGTTTGCCCGAAGGGT
CTGAACCCGACCAAGGCGATCGGCCACGTGCGTAATATGCTGCTGCAGAGCGGAACCTGA
Translation:
MLQVSVYRYNPDKDAAPYMQDFQVDTNGKDVMVLDVLALIKEQDEGFSYRRSCREGVCGSDGMNINGKNGLA
CITPLSAAGLKGGKLVIRPLPGLPVIRDLVVDMSIFYKQYEKVKPFLQNDTPAPAIERLQTPEEREKLDGLY
ECILCACCSTSCPSFWWNPDKFLGPAALLQAYRFLADSRDTKTEERLASLDDPFSVFRCRGIMNCVNVCPKG
LNPTKAIGHVRNMLLQSGT*
AnnotationID:3270GeneID:3262
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 06:06:01
Gene Name:sdhB
Confidence Code:2
GeneProduct:succinate dehydrogenase (B subunit)
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:succinate dehydrogenase, iron-sulfur protein
Functional Category Confidence Code:5
COGs:COG0479
Secondary Functional Category(ies):
EC Number:1.3.99.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55714.1| (AE016863) succinate dehydrogenase, iron-sulfur protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 234

 Score =  458 bits (1179), Expect = e-128
 Identities = 220/234 (94%), Positives = 227/234 (97%), Gaps = 1/234 (0%)

>gb|AAN69771.1|AE016613_6 (AE016789) succinate dehydrogenase, iron-sulfur protein
           [Pseudomonas putida KT2440]
          Length = 234

 Score =  451 bits (1161), Expect = e-126
 Identities = 215/235 (91%), Positives = 227/235 (96%), Gaps = 1/235 (0%)

>emb|CAA74088.1| (Y13760) succinate dehydrogenase putative iron sulphur subunit
           [Shewanella frigidimarina]
          Length = 235

 Score =  357 bits (915), Expect = 4e-98
 Identities = 164/232 (70%), Positives = 196/232 (84%), Gaps = 3/232 (1%)








 
Structural Features:
COG0479, FrdB, Succinate dehydrogenase/fumarate reductase, Fe-S protein subunit 
[Energy production and conversion]
CD-Length = 234 residues,  98.3% aligned
Score =  304 bits (781), Expect = 4e-84
Identities = 134/235 (57%), Positives = 159/235 (67%), Gaps = 6/235 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6527




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23669




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36507




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3262_cdd.html


    		




 
URL:

















    ReferenceID:36573




  
Author/Investigator(s):
        Darlison MG, Guest JR.





  
Title:
        Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli.




  
PubMed:
        6388571


MedLine:
        




    
Source:
Biochem J. 1984 Oct 15;223(2):507-17.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6388571

















    ReferenceID:36575




  
Author/Investigator(s):
        Phillips MK, Hederstedt L, Hasnain S, Rutberg L, Guest JR.





  
Title:
        Nucleotide sequence encoding the flavoprotein and iron-sulfur protein subunits of the Bacillus subtilis PY79 succinate dehydrogenase complex.




  
PubMed:
        3027051


MedLine:
        




    
Source:
J Bacteriol. 1987 Feb;169(2):864-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3027051









           
Homologs By Global Alignment 

           

       



     
Gene ID:3262
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5240
                           PA1584_tr
                           translation of PA1584
                           235
                           99.99
                           99.99
                           0
                           1257.0
     

     
     
146504
                           gb|AAG04973.1|AE004586_11
                           (AE004586) succinate dehydrogenase (B subunit) [Pseudomonas aeruginosa PAO1]
                           235
                           99.99
                           99.99
                           0
                           1257.0
     

     
     
146505
                           gb|AAO55714.1|
                           (AE016863) succinate dehydrogenase, iron-sulfur protein [Pseudomonas syringae pv. tomato str. DC3000]
                           235
                           93.61
                           96.59
                           1
                           1180.0
     

     
     
146506
                           gb|AAN69771.1|AE016613_6
                           (AE016789) succinate dehydrogenase, iron-sulfur protein [Pseudomonas putida KT2440]
                           235
                           91.48
                           96.59
                           1
                           1163.0
     

     
     
146510
                           emb|CAC89955.1|
                           (AJ414146) succinate dehydrogenase iron-sulfur protein [Yersinia pestis CO92]
                           239
                           70.29
                           81.17
                           5
                           911.0
     

     






      

          
GC ORFID: 46264How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1584Glimmer Score: 

          
Start: 1033680Stop: 1034387

          
Length: 708


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1584 translation of PA1584Homolog Bit Score 488.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene993 (ORF: BLASTX 457.0), t_PA1584 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTGCAAGTGAGTGTTTATCGTTACAACCCCGACAAAGATGCTGCGCCGTACATGCAGGATTTCCAGGTC 
GACACCAACGGCAAGGACGTGATGGTCCTCGACGTGCTGGCGCTGATCAAGGAACAGGACGAGGGCTTCTCC 
TACCGTCGTTCCTGCCGTGAAGGCGTCTGCGGCTCCGATGGCATGAACATCAACGGCAAGAACGGCCTGGCC 
TGCATCACCCCGCTGTCGGCCGCCGGCCTGAAGGGCGGCAAGCTGGTGATCCGTCCGCTGCCCGGCCTGCCG 
GTCATTCGTGACCTGGTCGTGGACATGAGCATCTTCTACAAGCAATACGAGAAGGTGAAGCCGTTCCTGCAG 
AACGATACGCCGGCCCCGGCCATTGAGCGTCTGCAGACCCCGGAAGAGCGCGAGAAGCTGGACGGTCTGTAC 
GAGTGCATCCTGTGCGCCTGCTGCTCGACCTCCTGCCCGTCCTTCTGGTGGAACCCCGACAAGTTCCTCGGT 
CCGGCCGCACTGCTGCAAGCCTATCGTTTCCTGGCCGACAGCCGTGACACCAAGACCGAAGAGCGCCTGGCT 
TCGCTGGACGACCCGTTCAGTGTGTTCCGCTGCCGCGGAATCATGAACTGCGTGAACGTTTGCCCGAAGGGT 
CTGAACCCGACCAAGGCGATCGGCCACGTGCGTAATATGCTGCTGCAGAGCGGAACCTGA